Survey of Total Tetrahydrophthalimide in Baby Foods Using Both Enzyme-Linked Immunosorbent Assay and Gas Chromatography/Mass Spectrometry: A Comparative Study

1993 ◽  
Vol 76 (6) ◽  
pp. 1225-1229 ◽  
Author(s):  
Jupiter M Yeung ◽  
W Harvey Newsome
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Baby Food ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay ◽  
Elisa Method ◽  
Baby Foods ◽  
Fruit Samples ◽  
Chromatographic Mass

Abstract An enzyme-linked immunosorbent assay (ELISA) method was compared with a gas chromatographic/ mass spectrometric (GC/MS) method for determining the concentration (in parts per million) of the combination of captan and its degradation product tetrahydrophthalimide (THPI) in 13 fruit samples and in a survey of baby foods. Ninety baby foods (49 fruits, 28 juices, and 13 vegetables) from 2 different suppliers were sampled. All captan in the samples was converted to THPI before each analysis. None of the samples contained a concentration of combined captan and THPI that violated the maximum residue limit of 5.0 ppm. Eight samples of baby food tested positive for THPI at levels ranging from 0.019-0.041 ppm by the GC/MS method, whereas 20 samples tested positive in the ELISA assay. All samples that tested positive with the GC/MS method also tested positive with the ELISA method. Thirteen percent of the baby food samples tested false positive with the ELISA method. The ELISA assay also gave higher values than the GC/MS method. The ELISA method can be effectively used as a primary screening tool to select samples testing positive for THPI. The concentration of THPI in these samples can then be verified using the GC/MS method.

Comparative Assessment of Fumonisin in Grain-Based Foods by ELISA, GC-MS, and HPLC

1994 ◽  
Vol 57 (2) ◽  
pp. 169-172 ◽  
Author(s):  
JAMES J. PESTKA ◽  
JUAN I. AZCONA-OLIVERA ◽  
RONALD D. PLATTNER ◽  
FIORENZA MINERVINI ◽  
M. BRUNO DOKO ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibody ◽  
Food Samples ◽  
Comparative Assessment ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cereal Samples ◽  
Related Compounds ◽  
Positive Results

Seventy-one (71) food samples were analyzed for the mycotoxin fumonisin by a monoclonal antibody based competitive enzyme-linked immunosorbent assay (ELISA). Fumonisins were detected primarily in corn-based products with 7/12, 2/2 and 1/3 and 1/7 yellow cornmeal, blue cornmeal, corn muffin mix, and mixed grain cereal samples yielding positive results, respectively. When the positive samples and randomly selected negative samples were assessed by other methods, correlations (r values) between ELISA and gas chromatography-mass spectrometry (GC-MS), ELISA and high-pressure liquid chromatography (HPLC) and GC-MS and HPLC were 0.478 (p < 0.05), 0.512 (p < 0.05), and 0.946 (p < 0.01), respectively. The results suggested that although the immunoassay could be used for screening of fumonisin in food samples, higher estimates were attained by ELISA than by the other two methods particularly in the more contaminated samples. These observations may result from differences in sample preparation among the methods or because of the presence of structurally related compounds in extracts that are detectable by ELISA but not the other two methods.

Quantification of human apolipoprotein A-IV by "sandwich"-type enzyme-linked immunosorbent assay.

1988 ◽  
Vol 34 (4) ◽  
pp. 739-743 ◽  
Author(s):  
M Rosseneu ◽  
G Michiels ◽  
W De Keersgieter ◽  
J Bury ◽  
J P De Slypere ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay ◽  
Chromatographic Fractionation ◽  
Human Apolipoprotein ◽  
Apolipoprotein A ◽  
Sandwich Type ◽  
Antibody Conjugate ◽  
The Mean

Abstract A specific and sensitive "sandwich"-type enzyme-linked immunosorbent assay (ELISA) has been developed for quantifying human apo A-IV. Using apo A-IV immunosorbent columns, we isolated monospecific anti-apo A-IV antibodies for coating the ELISA plates and for preparing peroxidase-antibody conjugate. The assay can detect as little as 0.20 ng of apo A-IV, with mean intra- and interassay CVs of 3.6% and 8.2%, respectively. The apoA-IV concentrations in normolipemic and hyperlipemic plasma were unaffected by either delipidation or treatment with detergents or urea. To validate the ELISA assay we compared it with an immunoelectrophoretic technique. ApoA-IV concentrations in plasma from normo- and dyslipemic subjects compared well by the two assays (r = 0.89). The mean apo A-IV concentration, measured by ELISA in plasma from 50 normolipemic subjects, was 143 (SD 52) mg/L; values for dyslipemic subjects were not significantly different. We also used this new assay to monitor apo A-IV profiles of normolipemic and hypertriglyceridemic plasma after chromatographic fractionation.

Evaluation of Performance of a Commercial Monoclonal Antibody–Based Fenitrothion Immunoassay and Application to Residual Analysis in Fruit Samples

2006 ◽  
Vol 69 (1) ◽  
pp. 191-198 ◽  
Author(s):  
EIKI WATANABE ◽  
KOJI BABA ◽  
HEESOO EUN ◽  
TOMOHITO ARAO ◽  
YASUO ISHII ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Close Correlation ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Recovery ◽  
Elisa Kit ◽  
Fruit Samples ◽  
Standard Solutions

The performance of a commercially available enzyme-linked immunosorbent assay (ELISA) kit containing highly sensitive monoclonal antibodies against fenitrothion was assessed. The experimentally estimated dynamic range (0.087 to 2 ng/g) agreed with that established by the kit manufacturer (0.075 to 1 ng/g). The linearity of the standard curve produced for the kit-assembled standard solutions (slope = −0.3829) agreed with that of the curve produced for the self-made standard solutions (slope =−0.3619). The sensitivity (I50 value) and the limit of detection for the kit were 0.23 and 0.033 ng/g, respectively. Selectivity of the ELISA indicates that the monoclonal antibody can readily distinguish the target pesticide from other structurally related analogs and some metabolites (oxon forms), with the exception of ethyl O-(p-nitrophenyl) phenyl phosphonothionate (EPN), parathion-methyl, and parathion. Methanol was the best organic solvent for the kit, with optimal sensitivity observed at a final concentration in the well of 10% (vol/vol) or less. Matrix interferences were minimized by direct dilution with water (60-fold) of the methanolic extracts from apple and peach samples. To extract fenitrothion from these two agricultural products as simply and rapidly as possible, three extraction methods were used. The extraction method that involved shaking by hand for 3 min was the best among the three methods. High recovery percentages (116.6% for apple and 110.8% for peach) were obtained. Validation of the ELISA method was carried out using the gas chromatography–mass spectrometry method, resulting in high recovery and close correlation of results (r > 0.95). These findings strongly suggest that the ELISA kit may be routinely employed for on-site fenitrothion screening of fruit samples.

Development of a new monoclonal antibody based direct competitive enzyme-linked immunosorbent assay for detection of brevetoxins in food samples

2010 ◽  
Vol 118 (2) ◽  
pp. 467-471 ◽  
Author(s):  
Yu Zhou ◽  
Yan-Song Li ◽  
Feng-Guang Pan ◽  
Yuan-Yuan Zhang ◽  
Shi-Ying Lu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay

Hapten Synthesis and Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay for Acrylamide in Food Samples

2014 ◽  
Vol 62 (29) ◽  
pp. 7078-7084 ◽  
Author(s):  
Jing Wu ◽  
Yu-Dong Shen ◽  
Hong-Tao Lei ◽  
Yuan-Ming Sun ◽  
Jin-Yi Yang ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Food Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hapten Synthesis

An enzyme-linked immunosorbent assay for the detection of apple mosaic virus in yellow birch

1980 ◽  
Vol 10 (3) ◽  
pp. 278-283 ◽  
Author(s):  
T. Hardcastle ◽  
A. R. Gotlieb
Keyword(s):  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Leaf Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Method ◽  
Yellow Birch ◽  
Field Samples

An enzyme-linked immunosorbent assay (ELISA) method was developed to detect apple mosaic virus (ApMV) in Betulaalleghaniensis Britton. Tests for virus using bark and bud tissues of dormant trees were successful. ApMV was also detectable in old leaf tissue in August and September, as well as in newly emerging leaf tissue forced in a greenhouse in March. Whole crude antiserum used to coat ELISA plates in tests with bud tissues was a reliable substitute for purified immunoglobulin without loss of sensitivity or specificity. An attempt was made to use ELISA for quantifying virus concentrations in field samples of ApMV-infected birch leaves.

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