Comparative Assessment of Fumonisin in Grain-Based Foods by ELISA, GC-MS, and HPLC

1994 ◽  
Vol 57 (2) ◽  
pp. 169-172 ◽  
Author(s):  
JAMES J. PESTKA ◽  
JUAN I. AZCONA-OLIVERA ◽  
RONALD D. PLATTNER ◽  
FIORENZA MINERVINI ◽  
M. BRUNO DOKO ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibody ◽  
Food Samples ◽  
Comparative Assessment ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cereal Samples ◽  
Related Compounds ◽  
Positive Results

Seventy-one (71) food samples were analyzed for the mycotoxin fumonisin by a monoclonal antibody based competitive enzyme-linked immunosorbent assay (ELISA). Fumonisins were detected primarily in corn-based products with 7/12, 2/2 and 1/3 and 1/7 yellow cornmeal, blue cornmeal, corn muffin mix, and mixed grain cereal samples yielding positive results, respectively. When the positive samples and randomly selected negative samples were assessed by other methods, correlations (r values) between ELISA and gas chromatography-mass spectrometry (GC-MS), ELISA and high-pressure liquid chromatography (HPLC) and GC-MS and HPLC were 0.478 (p < 0.05), 0.512 (p < 0.05), and 0.946 (p < 0.01), respectively. The results suggested that although the immunoassay could be used for screening of fumonisin in food samples, higher estimates were attained by ELISA than by the other two methods particularly in the more contaminated samples. These observations may result from differences in sample preparation among the methods or because of the presence of structurally related compounds in extracts that are detectable by ELISA but not the other two methods.

Gold Nanoparticles-Amplified Indirect Competitive Enzyme-Linked Immunosorbent Assay of BDE-121 Using a Monoclonal Antibody

2021 ◽  
Vol 21 (10) ◽  
pp. 5036-5043
Author(s):  
Yan Qiao ◽  
Qing-Yun Cai
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibody ◽  
Gold Nanoparticles ◽  
Immunosorbent Assay ◽  
Inhibitory Concentration ◽  
High Sensitivity ◽  
High Specificity ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzyme Linked Immunosorbent Assay ◽  
Good Recovery

In this study, we developed a monoclonal antibody against 2,3’,4,5’,6-pentabromodiphenylether (BDE-121) using a synthesized hapten, and established an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), using gold nanoparticles, to amplify the signal. The monoclonal antibody showed high specificity, with a half inhibitory concentration (IC50) value of 2.78 ng/mL, towards BDE-121. The developed IC-ELISA exhibited high sensitivity and stability as well as good recovery. The intra-assay deviation is below 6.8% and the inter-assay deviations range from 6.5% to 8.7%. The assay of the actual samples was found to be consistent with those of gas chromatography/mass spectrometry (GC/MS).

scholarly journals GC-MS analysis of Adulterants in Captagon Tablet

2020 ◽  
Vol 1 (1) ◽  
pp. 14-21
Author(s):  
Ali Zaid Alshehri ◽  
Mohammed saeed Al Qahtani ◽  
Mohammed Aedh Al Qahtani ◽  
Abdulhadi M Faeq ◽  
Jawad Aljohani ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Middle East ◽  
General Purpose ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Active Compounds ◽  
Other Substances ◽  
Positive Results ◽  
Dimethyl Polysiloxane

In secret laboratories various synthetic or semi-synthetic amphetamine-type stimulants (ATS) are produced and processed. Stimulants of the form of amphetamine (ATS) are the second most common illegal drugs used worldwide, following cannabis. ATS production has increased worldwide, particularly from the Middle East. Fenethylline (CaptagonTM), a derivative of amphetamine, is commonly marketed as a street drug. Objective: this research aimed at applying gas chromatography / mass spectrometry (GC / MS) for fenethylline profiling of confiscated samples from the Saudi market. Methodology: The study of GC / MS was conducted on a general-purpose column (30 mm 0.25 mm i.d) filled with 0.25 mm cross bond, 5% diphenyl dimethyl polysiloxane (Rtx-5MS). The mass was worked in the process of impacting the electron. Results and discussion: Analyzed samples showed positive results for amphetamine in concentrations ranging from 0.07 to 43.02 percent of the substance in 60 percent of the samples. Caffeine was also present in 60 per cent of the samples at levels between 0.61 and 60.31 per cent. In the MS of the other active compounds were recorded in different proportions. Lastly, the presence of other substances in fenethylline samples may contribute to intoxication.

scholarly journals Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit

2006 ◽  
Vol 13 (3) ◽  
pp. 420-422 ◽  
Author(s):  
S. E. Burastero ◽  
C. Paolucci ◽  
D. Breda ◽  
G. Monasterolo ◽  
R. E. Rossi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
False Positive ◽  
Positive Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Results

ABSTRACT The Bühlmann CAST 2000 enzyme-linked immunosorbent assay is a potentially useful assay for measuring sulfidoleukotrienes released in vitro by allergen-challenged basophils. However, we observed that the positive-control reagent yielded positive signals in cell-free systems. These false-positive results depended on using a mouse anti-FcεRI monoclonal antibody and were prevented by degranulation-inducing reagents other than mouse monoclonal antibodies.

Catalytic Pyrolysis of Waste Achyranthes Root Over Al-MCM-41

2021 ◽  
Vol 21 (7) ◽  
pp. 4081-4084
Author(s):  
Seul-Bee Lee ◽  
Young-Min Kim ◽  
Ji-Hui Park ◽  
Young-Kwon Park
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Aromatic Hydrocarbons ◽  
Catalytic Pyrolysis ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Acidic Properties ◽  
Achyranthes Root ◽  
Formation Efficiency ◽  
Mcm 41

This study examined the thermal and catalytic pyrolysis of waste Achyranthes Root (AR) using pyrolyzer-gas chromatography/mass spectrometry (Py-GC/MS). The non-catalytic pyrolysis of waste AR produced various kinds of oxygenates, such as acetic acid, hydroxy propanone, furfural, phenol, cresol, guaiacols, syringols, and so on. By applying nanoporous Al-MCM-41 with acidic properties and mesopores to the pyrolysis of waste AR, the levels of furan and aromatic hydrocarbons production increased with a concomitant decrease in the other oxygenates. The formation efficiency of furans was improved further by increasing the amount of Al-MCM-41 applied to the catalytic pyrolysis of waste AR.

Extraction and Characterization of Gibberellins from Hordeum vulgare L. Seedlings

1974 ◽  
Vol 1 (2) ◽  
pp. 183 ◽  
Author(s):  
KF Faull ◽  
BG Coombe ◽  
LG Paleg
Keyword(s):  
Mass Spectrometry ◽  
Dry Weight ◽  
Paper Electrophoresis ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Gas Liquid Chromatography ◽  
Fresh Weight ◽  
Hordeum Vulgare L ◽  
Layer Chromatography

Two gibberellins, one GA1-like, the other GA3-like, were identified in the extracts of roots and tops of 8-,11- and 15-day-old barley seedlings by paper chromatography, paper electrophoresis, thin-layer chromatography, gas-liquid chromatography and bioassay procedures, followed by combined gas chromatography-mass spectrometry. The amounts of gibberellins in the seedlings ranged from 7 to 11 ng per plant. The concentrations of gibberellins in the seedlings were 32-320 ng/g dry weight and 5-28 ng/g fresh weight; concentrations in the roots were higher than those in the shoots.

Determination of Polychlorodibenzo-p-dioxins and Related Compounds in Commercial Chlorophenols

1972 ◽  
Vol 55 (1) ◽  
pp. 85-92 ◽  
Author(s):  
David Firestone ◽  
John Ress ◽  
N L Brown ◽  
R P Barron ◽  
J N Damico
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Electron Capture ◽  
Gas Chromatography Mass Spectrometry ◽  
Alumina Column ◽  
Spectrometric Data ◽  
Chromatography Mass Spectrometry ◽  
Related Compounds ◽  
Chromatographic Mass

Abstract Twenty-one commercial chlorophenols were examined for the presence of polychlorodibenzo- p-dioxins (chlorodioxins) and related compounds. The chlorophenols were dissolved in aqueous alkali, extracted with petroleum ether, and fractionated on an alumina column. Alumina fractions were examined by electron capture gas chromatography and combined gas chromatography-mass spectrometry. Chlorodioxin content was estimated by electron capture gas chromatography. The presence of chlorodioxins, polychlorodibenzofurans (chlorofurans), and polychlorodiphenyl ethers (chloroethers) was confirmed by combined gas chromatography-mass spectrometry. The 2,3,-7,8-tetrachlorodioxin was found in 3 of 6 samples of 2,4,5-trichlorophenol but was not detected in any of the 11 samples of tetra- and pentachlorophenol that were examined. Hexachlorodioxin was present at levels ranging from 0.17 to 39 ppm in all 8 pentachlorophenols examined. Hexa-, hepta-, and octachlorodioxins as well as a wide variety of chlorofurans and chloroethers of varying chlorine content were present in most of the tetra- and pentachlorophenols. In addition, the gas chromatographic- mass spectrometric data suggested that some of the chlorophenols contained methoxy- and dimethoxypolychlorofurans and methoxypolychloroethers as well as polychlorohy droxybiphenyl.

scholarly journals Essential Oil Constituents of Eclipta Prostrata (L.) L. and Vernonia amygdalina Delile

2009 ◽  
Vol 4 (3) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Akinola O. Ogunbinu ◽  
Guido Flamini ◽  
Pier L. Cioni ◽  
Isiaka A. Ogunwande ◽  
Sunday O. Okeniyi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Composition ◽  
Stem Bark ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Vernonia Amygdalina ◽  
Straight Chain ◽  
Aerial Parts ◽  
Eclipta Prostrata

The chemical composition of the essential oils from the leaves and stem bark of Eclipta prostrata (L.) L. and the aerial parts of Vernonia amygdalina Delile (Asteraceae) have been analyzed by capillary gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). The investigation led to the identification of 33 and 30 compounds in the oils of the leaves and stem of E. prostrate, respectively, and 40 compounds in the oil of V. amygdalina. While the oil of the leaves of E. prostrata was highly dominated by sesquiterpenoids (89.3%), the stem bark was comprised of sesquiteprenoids (47.7%), straight chain hydrocarbons (25.6%) and monoterpenoids (11.1%). The main constituents of both oils were β-caryophyllene (47.7% and 15.9%) and α-humulene (31.8 and 12.9%) in the leaves and stem, respectively. In addition, ( E)-β-farnesene (10.0%) was also identified in significant amount in the stem bark. On the other hand, the major component of V. amygdalina oil was α-muurolol (45.7%).

Phenolic Composition of Bud Exudates of Populus deltoides

1990 ◽  
Vol 45 (6) ◽  
pp. 587-593 ◽  
Author(s):  
W. Greenaway ◽  
S. English ◽  
F. R. Whatley
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Methyl Ether ◽  
Principal Components ◽  
North American ◽  
Populus Deltoides ◽  
Gas Chromatography Mass Spectrometry ◽  
Phenolic Composition ◽  
American States ◽  
Related Compounds

Abstract Bud exudate of Populus deltoides clones originating from six central and eastern American states was examined by gas chromatography/mass spectrometry and the components were identified. The principal components of the bud exudate were the flavone galangin, the flavanone pinocembrin and the flavanonol pinobanksin, together with the related compounds pinocembrin chalcone, pinobanksin methyl ether and pinobanksin-3-acetate. The bud exudate composition was very different from that seen in a North American poplar of the section Tacamahaca, P. balsamifera. The bud exudates from all P. deltoides specimens were similar excepting that from Vermont.

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