scholarly journals UV Spectrophotometry and Nonaqueous Determination of Terbinafine Hydrochloride in Dosage Forms

1999 ◽  
Vol 82 (4) ◽  
pp. 830-833 ◽  
Author(s):  
Simone Gonçalves Cardoso ◽  
Elfrides E S Schapoval
Keyword(s):  
High Performance ◽  
Raw Materials ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Volumetric Method ◽  
Uv Spectrophotometry ◽  
Terbinafine Hydrochloride ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract An ultraviolet spectrophotometric and a nonaqueous volumetric method for determining terbinafine hydrochloride (TH) in pharmaceutical formulations are presented. The UV spectrophotometric procedure was developed for assay of TH in raw materials, tablets, and creams. The method was tested for linearity (0.8–2.8 μg/mL, r = 0.9997), recovery (102.00% for creams and 99.90% for tablets) and precision (101.3%, CV = 0.96%, n = 9, for creams; 100.25%, CV = 1.08%, n = 9, for tablets). The volumetric method involves titration of TH with 0.05M perchloric acid with crystal violet as indicator. This method was used for quantitative determination of TH in raw materials and tablets. Mean recovery and precision were, respectively, 100.41 and 101.18% (CV = 1.64%, n = 9) for TH in tablets. There were no significant differences between the proposed methods and a previously described high-performance liquid chromatographic method. The UV spectrophotometric and titrimetric methods are potentially useful for a routine laboratory because of their simplicity, rapidity, and accuracy.

Method Development and Validation of Propofol by Reverse Phase HPLC and its Estimation in Commercial Formulation

2021 ◽  
pp. 3139-3142
Author(s):  
Kuntal Mukherjee ◽  
S. T. Narenderan ◽  
B. Babu ◽  
Survi Mishra ◽  
S. N. Meyyanathan
Keyword(s):  
High Performance ◽  
Method Development ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Reverse Phase Hplc ◽  
Liquid Chromatographic Method ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Liquid Chromatographic

A simple, sensitive and rapid high performance liquid chromatographic method has been developed for the determination of Propofol. The main focus of the method was to determine Propofol in solution form as well as in marketed formulation. Chromatographic separation was achieved on Inertsil ODS-3V column (250mm x 4.6mm; 5µm) with a mobile phase consisting of methanol: water (85:15), with a flow rate of 1.0ml/min (UV detection at 270nm). Linearity was observed over the concentration range of 10-110µg/ml with a regression equation y=88048x + 44524 and having a regression value (R2) of 0.999. The LOD and LOQ values found to be 10ng and 100ng, respectively. No changes found in ruggedness and robustness studies. The percentage of recovery was found to be 95.25% to 101.81%. Validation studies revealed that the method was specific, accurate, precise, reliable, robust, reproducible and suitable for the quantitative analysis in its pharmaceutical formulations.

Development and Validation of Midostaurin Assay by RP-HPLC Method

2018 ◽  
Vol 11 (6) ◽  
pp. 4318-4322
Author(s):  
Abrar Ahmed ◽  
Tayyaba Mahtab ◽  
Sumaiyya Saleem
Keyword(s):  
Myeloid Leukemia ◽  
High Performance ◽  
Kinase Inhibitor ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Liquid Chromatographic Method ◽  
Development And Validation ◽  
Liquid Chromatographic

Midostaurin is a multi-targeted protein kinase inhibitor that has been used for the treatment of acute myeloid leukemia.  Here, a rapid and precise reverse phase high-performance liquid chromatographic method has been developed for the validation of midostaurin, in its API form as well as in capsule dosage form. Chromatography was carried out on a X-Bridge C18 (4.6 x 250 mm, 5 µm) column using a mixture of methanol: water (75:25% v/v) as the mobile phase at a flow rate of 1.0 mL/min, the detection was carried out at 243nm and the retention time of the midostaurin was found to be 3.155. The method produce linear responses in the concentration range of 10-50 µg/mL of midostaurin. The method precision for the determination of assay was below 2.0 % RSD. The LOD and LOQ values obtained were 1.2 µg/mL and 3.8 µg/mL respectively. There were no significant changes observed upon changing chromatographic conditions indicating the method to be robust. Therefore this validated method can be useful in the quality control of bulk and pharmaceutical formulations of midostaurin. 

scholarly journals A Reversed-Phase High-Performance Liquid Chromatographic Method for the Determination of Zafirlukast in Pharmaceutical Formulations and Human Plasma

2006 ◽  
Vol 89 (6) ◽  
pp. 1557-1564 ◽  
Author(s):  
İncilay SÜsÜl ◽  
Sacide AltinÖz
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Chromatographic Method ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Liquid Chromatographic Method ◽  
Spiked Plasma ◽  
Liquid Chromatographic

Abstract Zafirlukast (ZAF) is a leukotriene receptor antagonist used in the treatment of chronic asthma. In this study, a simple and sensitive reversed-phase, high-performance liquid chromatographic method was developed for the determination of ZAF in pharmaceutical formulations and human plasma. Piribedil was used as an internal standard. Analysis was carried out on a Nucleosil C18 100 Å(150 × mm 4.6 mm id, 5 ωm) column with acetonitrilepH 3.0 acetate buffer (70 30, v/v) as the mobile phase at a flow rate of 0.8 mL/min. The peak was detected by an ultraviolet detector set at a wavelength of 240 nm. The retention times were about 3.9 min for piribedil and 5.8 min for ZAF. The developed method was applied to the determination of ZAF in its pharmaceutical formulation and spiked human plasma. For quantification of ZAF in spiked plasma, proteins were precipitated with ethanol before chromatographic analysis. The calibration range was linear from 49.69437.50 ng/mL in spiked plasma. The absolute recovery from spiked plasma was 98.73 ± 0.42% at a concentration of 254.78 ng/mL of ZAF. No endogenous substances from plasma were found to interfere.

scholarly journals Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation

2010 ◽  
Vol 7 (1) ◽  
pp. 117-122 ◽  
Author(s):  
S. K. Patro ◽  
S. R. Swain ◽  
V. J. Patro ◽  
N. S. K. Choudhury
Keyword(s):  
High Performance ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Liquid Chromatographic Method ◽  
Tablet Formulations ◽  
Development And Validation ◽  
Liquid Chromatographic

A new, simple, specific, accurate and precise RP-HPLC method was developed for determination of lamivudine in pure and tablet formulations. A Thermo BDS C18 column in isocratic mode, with a mobile phase consisting of 0.01 M ammonium dihydrogen orthophosphate buffer adjusted to pH 2.48 by using formic acid and methanol in the ratio of 50:50 was used. The flow rate was set at 0.6 mL/min and UV detection was carried out at 264 nm. The retention time of lamivudine and nevirapine were 2.825 min and 4.958 min respectively. The method was validated for linearity, precision, robustness and recovery. Linearity for lamivudine was found in the range of 50-175 μg/mL. Hence, it can be applied for routine quality control of lamivudine in bulk and pharmaceutical formulations.

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