50 3D Printing of Dermal ECM Hyfrogel Enhances the Therapeutic Effects of Split Thickness Skin Grafting in Full-thickness Skin Wound Repair

Nanofibrillar cellulose (NFC)-derived dressings such as films, hydrogels, and aerogels are one of the favorable materials for wound healing due to their proper mechanical properties and water holding ability. However, the therapeutic differences between native and anionic NFC materials are rarely studied. In this report, we compared the differences and addressed the regenerative potential of native and anionic wood-derived NFC hydrogels for wound treatment. In vitro characteristics of the hydrogels were detected using scanning electron microscopy, rheological measurements, and swelling and hemolytic activity assays. Skin regeneration at an early stage after hydrogel treatment was analyzed using an in vivo splinted excisional full-thickness skin wound model in C57BL/6 mice. Both native NFC and anionic NFC (ANFC) hydrogel with differing mechanical and surface properties were shown to be biocompatible. Surprisingly, wounds treated with NFC and ANFC hydrogel did not show any statistical difference compared with control wounds and progressed through normal wound closure, inflammatory response, re-epithelialization, vascularization, and tissue maturation with no signs of fibrosis. The data show here for the first time the therapeutic performance of native and anionic NFC hydrogel in a wound mimicking human wound healing mechanisms. The mechanical properties of native and anionic NFC hydrogels such as the capability to modify material stiffness may also prove to be valuable in the management of wounds in the future.

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Prevascularized Stem Cell Sheet for Full-Thickness Skin Wound Repair

Recent Clinical Techniques, Results, and Research in Wounds - Vascular Surgery, Neurosurgery, Lower Extremity Ulcers, Antimicrobials, Wound Assessment, Care, Measurement and Repair ◽

10.1007/15695_2017_86 ◽

2018 ◽

pp. 167-172 ◽

Cited By ~ 1

Author(s):

Daniel Radke ◽

Lei Chen ◽

Shaohai Qi ◽

Feng Zhao

Keyword(s):

Stem Cell ◽

Wound Repair ◽

Cell Sheet ◽

Skin Wound ◽

Full Thickness ◽

Full Thickness Skin ◽

Thickness Skin

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One-step approach for full-thickness skin defect reconstruction in rats using minced split-thickness skin grafts with Pelnac overlay

Burns & Trauma ◽

10.1186/s41038-019-0157-0 ◽

2019 ◽

Vol 7 ◽

Cited By ~ 2

Author(s):

Tong Liu ◽

Chao Qiu ◽

Chi Ben ◽

Haihang Li ◽

Shihui Zhu

Keyword(s):

Wound Healing ◽

Healing Rate ◽

Skin Grafting ◽

Skin Defect ◽

Skin Grafts ◽

Full Thickness ◽

Full Thickness Skin ◽

Thickness Skin ◽

Split Thickness Skin Grafting

Abstract Background Split-thickness skin grafting is the current gold standard for the treatment of traumatic skin loss. However, for patients with extensive burns, split-thickness skin grafting is limited by donor skin availability. Grafting split-thickness skin minced into micrografts increases the expansion ratio but may reduce wound repair quality. Dermal substitutes such as Pelnac can enhance the healing of full-thickness skin wounds, but their application currently requires two surgeries. The present study investigated whether it is possible to repair full-thickness skin defects and improve wound healing quality in a single surgery using Pelnac as an overlay of minced split-thickness skin grafts in a rat model. Methods A full-thickness skin defect model was established using male Sprague-Dawley rats of 10 weeks old. The animals were randomly divided into control and experimental groups in which Vaseline gauze and Pelnac, respectively, were overlaid on minced split-thickness skin grafts to repair the defects. Wound healing rate and quality were compared between the two groups. For better illustration of the quality of wound healing, some results were compared with those obtained for normal skin of rats. Results We found that using Pelnac as an overlay for minced split-thickness skin grafts accelerated wound closure and stimulated cell proliferation and tissue angiogenesis. In addition, this approach enhanced collagen synthesis and increased the formation of basement membrane and dermis as well as the expression of growth factors related to wound healing while reducing scar formation. Conclusions Using minced split-thickness skin grafts overlaid with Pelnac enables the reconstruction of full-thickness skin defects in a single step and can increase the healing rate while improving the quality of wound healing.

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Bubaline Cholecyst Derived Extracellular Matrix for Reconstruction of Full Thickness Skin Wounds in Rats

Scientifica ◽

10.1155/2016/2638371 ◽

2016 ◽

Vol 2016 ◽

pp. 1-13 ◽

Cited By ~ 2

Author(s):

Poonam Shakya ◽

A. K. Sharma ◽

Naveen Kumar ◽

Remya Vellachi ◽

Dayamon D. Mathew ◽

...

Keyword(s):

Extracellular Matrix ◽

Wound Repair ◽

Healing Process ◽

Skin Wound ◽

Skin Wounds ◽

Full Thickness ◽

Open Wound ◽

Full Thickness Skin ◽

Group I ◽

Thickness Skin

An acellular cholecyst derived extracellular matrix (b-CEM) of bubaline origin was prepared using anionic biological detergent. Healing potential of b-CEM was compared with commercially available collagen sheet (b-CS) and open wound (C) in full thickness skin wounds in rats. Thirty-six clinically healthy adult Sprague Dawley rats of either sex were randomly divided into three equal groups. Under general anesthesia, a full thickness skin wound (20 × 20 mm2) was created on the dorsum of each rat. The defect in group I was kept as open wound and was taken as control. In group II, the defect was repaired with commercially available collagen sheet (b-CS). In group III, the defect was repaired with cholecyst derived extracellular matrix of bovine origin (b-CEM). Planimetry, wound contracture, and immunological and histological observations were carried out to evaluate healing process. Significantly (P<0.05) increased wound contraction was observed in b-CEM (III) as compared to control (I) and b-CS (II) on day 21. Histologically, improved epithelization, neovascularization, fibroplasia, and best arranged collagen fibers were observed in b-CEM (III) as early as on postimplantation day 21. These findings indicate that b-CEM have potential for biomedical applications for full thickness skin wound repair in rats.

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Injectable Self-Healing Ceria-based Nanocomposite Hydrogel with ROS-scavenging Activity for Skin Wound Repair

Regenerative Biomaterials ◽

10.1093/rb/rbab074 ◽

2021 ◽

Author(s):

Xueyun Gong ◽

Meng Luo ◽

Min Wang ◽

Wen Niu ◽

Yidan Wang ◽

...

Keyword(s):

Wound Healing ◽

Wound Repair ◽

Skin Wound ◽

Scavenging Activity ◽

Self Healing ◽

Nanocomposite Hydrogel ◽

Full Thickness ◽

Ros Scavenging ◽

Full Thickness Skin ◽

Thickness Skin

Abstract Excessive reactive oxygen species (ROS) in the injured skin may impede the wound repair and skin regeneration. Herein, we develop an injectable self-healing ceria-based nanocomposite hydrogel with ROS-scavenging activity to accelerate wound healing. The nanocomposite hydrogels were successfully prepared by coating cerium oxide nanorods with polyethylenimine (PEI) and crosslinked with benzaldehyde-terminated F127 (F127-CHO) through the dynamic Schiff-base reaction (FVEC hydrogel). The results showed that the FVEC hydrogel possessed the good thermosensitivity, injectability, self-healing ability and ROS scavenging activity. The subcutaneous implantation experiments in mice confirmed that FVEC hydrogels are biocompatible and biodegradable in vivo. The full-thickness skin wound studies showed that FVEC hydrogel could significantly enhance the wound healing and epithelium regeneration with the formation of hair follicle and adipocyte tissue. This work provides a new strategy for the development of multifunctional Ce-based nanocomposite hydrogel for full-thickness skin wound healing and regeneration.

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First experiences with a new surgical approach in adult full-thickness burns: single step reconstruction of epidermal, dermal and subcutaneous defects by use of split-thickness skin grafting, a dermal collagen matrix and autologous fat-transfer

European Surgery ◽

10.1007/s10353-013-0211-2 ◽

2013 ◽

Vol 45 (5) ◽

pp. 263-269 ◽

Cited By ~ 5

Author(s):

M. Keck ◽

H. F . Selig ◽

J. Kober ◽

D. B . Lumenta ◽

H. Schachner ◽

...

Keyword(s):

Skin Grafting ◽

Collagen Matrix ◽

Single Step ◽

Full Thickness ◽

Fat Transfer ◽

Autologous Fat ◽

Autologous Fat Transfer ◽

Thickness Skin ◽

Split Thickness Skin Grafting ◽

Dermal Collagen

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Early wound repair versus later scar repair in children with treadmill hand friction burns

Journal of Burn Care & Research ◽

10.1093/jbcr/irab083 ◽

2021 ◽

Author(s):

Rong Zhou ◽

Lin Qiu ◽

Jun Xiao ◽

Xiaobo Mao ◽

Xingang Yuan

Keyword(s):

Skin Graft ◽

Wound Repair ◽

Full Thickness ◽

Full Thickness Skin Graft ◽

Vacuum Sealing ◽

Full Thickness Skin ◽

Thickness Skin ◽

Vacuum Sealing Drainage ◽

Repair Group ◽

Hand Friction

Abstract The incidence of pediatric treadmill hand friction burns has been increasing every year. The injuries are deeper than thermal hand burns, the optimal treatment remains unclear. This was a retrospective study of children who received surgery for treadmill hand friction burns from January 1, 2015, to December 31, 2019, in a single burn center. A total of 22 children were surveyed. The patients were naturally divided into two groups: the wound repair group (13 patients), which was admitted early to the hospital after injury and received debridement and vacuum sealing drainage initially, and a full-thickness skin graft later; and the scar repair group (9 patients), in which a scar contracture developed as a result of wound healing and received scar release and skin grafting later. The Modified Michigan Hand Questionnaire score in the wound repair group was 116.31 ± 10.55, and the corresponding score in the scar repair group was 117.56 ± 8.85 (P>0.05), no statistically significant difference. The Vancouver Scar Scale score in the wound repair group was 4.15 ± 1.21, and the corresponding score in the scar repair group was 7.22 ± 1.09 (P<0.05). Parents were satisfied with the postoperative appearance and function of the hand. None in the two groups required secondary surgery. If the burns are deep second degree, third degree, or infected, early debridement, vacuum sealing drainage initially, and a full-thickness skin graft can obviously relieve pediatric pain, shorten the course of the disease, and restore the function of the hand as soon as possible.

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