Modifiable Washing-Flotation Method for Separation of Insect Eggs and Larvae from Soil1

1979 ◽  
Vol 72 (1) ◽  
pp. 67-69 ◽  
Author(s):  
M. E. Montgomery ◽  
G. J. Musick ◽  
J. B. Polivka ◽  
D. G. Nielsen
Keyword(s):  
Insect Eggs ◽  
Flotation Method ◽  
Eggs And Larvae

Versatile Method for Staining Insect Eggs and Larvae in Leaves

1988 ◽  
Vol 81 (1) ◽  
pp. 403-405 ◽  
Author(s):  
Krishna P. N. Moorthy ◽  
M. P. Alexander ◽  
G. C. Tewari
Keyword(s):  
Insect Eggs ◽  
Eggs And Larvae

scholarly journals Biología del Picudo de la guayaba Conotrachelus psidii (Marshall) (Coleoptera: Curculionidae)

2007 ◽  
Vol 7 (2) ◽  
pp. 73 ◽  
Author(s):  
Rafael Augusto Monroy ◽  
Orlando Ildefonso Insuasty
Keyword(s):  
Morphological Changes ◽  
Design Strategies ◽  
Insect Eggs ◽  
Guava Fruit ◽  
Larval Stages ◽  
Light Yellow ◽  
Yellow Color ◽  
Rain Season ◽  
Light Yellow Color ◽  
Eggs And Larvae

<p>El Picudo, <em>Conotrachelus psidii </em>Marshall, desarrolla su estado larvario en el fruto de la guayaba alimentándose de la semilla; el insecto petrifica y madura prematuramente la fruta confiriéndole un aspecto desagradable que causa rechazo en el mercado. En la actualidad causa pérdidas significativas en la agroindustria de la Hoya de Río Suárez. Conocer la duración de los estadios biológicos y sus preferencias de oviposición, permitirá diseñar estrategias de manejo integrado. Los huevos y las larvas se obtuvieron semanalmente de frutos infestados artificialmente para conocer los cambios morfológicos de la cabeza de la larva y la duración del estadio. Cuando las larvas abandonaron el fruto se introdujeron en dispositivos plásticos tubulares saturados con tierra, los cuales se disectaron mensualmente para registrar los estadios de larva, pupa y adulto en el suelo. Se observó que las hembras ovipositaron frutos de 30 a 90 días de edad; los huevos son blanquecinos y con longitud promedio de 1 mm. La larva es ápoda y de color amarillo, con longitudes entre 1,2 y 1,5 mm y entre 10 y 12 mm en las semanas primera y sexta, respectivamente. La pupa es exarata, amarilla clara y de 7,5 mm de longitud. El adulto es café oscuro y tiene 6 mm de largo. El ciclo total duró 199 días distribuido así: huevo, 4 a 7 días; larva en el fruto, 42 a 56 días; larva en suelo, 90 días; pupa, 30 a 60 días. Los adultos emergieron entre 20 y 30 días en la época lluviosa y en laboratorio sobrevivieron hasta 435 días después de su emergencia.</p><p> </p><p><strong>Biology of the guaba weevil <em>Conotrachelus psidii </em>(Marshall) (Coleoptera: Curculionidae)</strong></p><p>The weevil <em>Conotrachelus psidii </em>(Marshall) develops its larval stages inside the guava fruit feeding on its seeds, and petrifying and prematurely ripening the fruit, developing an unpleasant appearance, rejected by the market. To design strategies for biological control, its necessary to determine the length of the different biological phases of the insect. Eggs and larvae were obtained every week from semi-artificially infected fruits to determine larva’s head morphological changes. Once larvae abandoned the fruit, they were transferred to 6 “ diameter plastic tubes filled with soil and dissected monthly to register the different life stages of larvae, pupae and adults. Oviposition preferences were observed in 30 to 90 days old guavas. Eggs are whitish and measured on average 1mm of length. Larvae are apoda and yellow, ranging in length from 1.2 to 1.5 mm and from 10 to 12 mm in the first and sixth week respectively. Pupae have exharate shape, of light yellow color with an average of 7.5 mm length. Adults are dark brown and 6 mm in length. The total cycle lasted 199 days distributed as follow: egg, 4 to 7 days; larva in the fruit, 42 to 56 days; larva in soil, 90 days and pupa stage from 30 to 60 days. Adults emerged from 20 to 30 days during the rain season and survive up to 435 days under laboratory conditions.</p><p> </p>

scholarly journals The Use of Saturated MgSO4 Compared to Saturated Sucrose in the Flotation Method to Identify Eggs and Larvae of Soil-Transmitted Helminths from Plantation Soil

2021 ◽  
Vol 7 (2) ◽  
pp. 65
Author(s):  
Nidya Husna Kholidah ◽  
Yunita Armiyanti ◽  
Dwita Aryadina Rachmawati ◽  
Bagus Hermansyah ◽  
Yudha Nurdian
Keyword(s):  
Sucrose Solution ◽  
Soil Samples ◽  
Wilcoxon Test ◽  
Residential Areas ◽  
Soil Transmitted Helminths ◽  
Three Samples ◽  
Soil Media ◽  
Flotation Method ◽  
The Many ◽  
Eggs And Larvae

Worms infection is still common in Indonesia with a prevalence that varies around 40% -60% at all ages. This disease can be caused by infection of Soil-Transmitted Helminths (STH) that require soil media to infect humans. The many plantation lands in Jember are a suitable environment for the development of STH and STH identification in soil can be done by flotation method using MgSO4 solution or sucrose. The comparison of the effectiveness of the MgSO4 solution with the sucrose solution has not been widely studied. Therefore, the aim of this study was to compare the efficacy of using saturated MgSO4 solution with saturated sucrose to identify eggs and larvae of STH in Sumber Wadung plantation soil, Hargomulyo Village, Silo District, Jember Regency using the flotation method. Soil samples were taken as many as 35 samples in the garden area, 35 samples around the river, and 35 samples in residential areas,. The results of identification of STH eggs and larvae obtained Ascarid eggs (1.9%) and Hookworm larvae (0.01%). The number of positive soil samples containing STH eggs and larvae using different solutions is the same as three samples (0.02%).The Wilcoxon test results showed that there was no difference in the results of using the MgSO4 solution with the sucrose solution in the flotation method (p> 0.05) so that the MgSO4 solution and sucrose solution could be used to detect eggs and larvae of STH worms and had the same effectiveness. Keyword: Soil-transmitted helminths, saturated MgSO4,  saturated sucrose, flotation

Recent demonstration of helminth eggs and larvae from vegetable cultivating soil

1982 ◽  
Vol 20 (2) ◽  
pp. 83 ◽  
Author(s):  
Dong Wik Choi ◽  
Mee Sun Ock ◽  
Jong Woo Suh
Keyword(s):  
Helminth Eggs ◽  
Eggs And Larvae ◽  
Recent Demonstration

CURRENT STATE AND DEVELOPMENT PERSPECTIVES OF THE CASPIAN SPRAT FISHERIES

2018 ◽  
pp. 69-75
Author(s):  
Tatiana Vasilievna Pomogaeva ◽  
Aliya Ahmetovna Aseinova ◽  
Yuriy Aleksandrovich Paritskiy ◽  
Vjacheslav Petrovich Razinkov
Keyword(s):  
Food Item ◽  
Growth Potential ◽  
North West ◽  
Marine Fishery ◽  
The North ◽  
Current State ◽  
West Part ◽  
Eggs And Larvae ◽  
Intensive Growth

The article presents annual statistical data of the Caspian Research Institute of Fishery. There has been kept track of the long term dynamics of the stocks of three species of Caspian sprat (anchovy, big-eyed kilka, sprat) and investigated a process of substituting a food item of sprats Eurytemora grimmi to a small-celled copepod species Acartia tonsa Dana. According to the research results, there has been determined growth potential of stocks of each species. Ctenophoran-Mnemiopsis has an adverse effect on sprat population by eating fish eggs and larvae. Ctenophoram - Mnemiopsis is a nutritional competitor to the full-grown fishes. The article gives recommendations on reclamation of stocks of the most perspective species - common sprat, whose biological characteristics helped not to suffer during Ctenophoram outburst and to increase its population during change of the main food item. Hydroacoustic survey data prove the intensive growth of common sprat biomass in the north-west part of the Middle Caspian. According to the results of the research it may be concluded that to realize the volumes of recommended sprat catch it is necessary to organize the marine fishery of common sprat at the Russian Middle Caspian shelf.

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