Insecticide Resistance of Diamondback Moth (Lepidoptera: Plutellidae) in North America

During studies of the parasite complex of the diamondback moth, Plutella maculipennis Curt., at Ottawa, Ontario, July to October, 1952, and April, 1953, 1,234 host cocoons were collected from a heavily infested field of Penn State Ballhead cabbage. On October 31, eight adults (2 ♂ and 6 ♀) of Tetrastichus sokolowskii Kurdj., emerged from a single cocoon collected 16 days previously and held under laboratory conditions in a gelatin capsule. On May 10, nine adults (1 ♂ and 8 ♀) and on May 15, eight adults (1 ♂ and 7 ♀) of the same species emerged from two overwintered cocoons collected 14 and 16 days previously. In each case, the lack of parasitic remnants indicated that they were primary parasites.

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The role of non-specific esterases in insecticide resistance to malathion in the diamondback moth Plutella xylostella

Comparative Biochemistry and Physiology Part C Comparative Pharmacology ◽

10.1016/0742-8413(89)90014-5 ◽

1989 ◽

Vol 93 (1) ◽

pp. 81-85 ◽

Cited By ~ 7

Author(s):

K. Doichuanngam ◽

R.A. Thornhill ◽

Mark S. Myers

Keyword(s):

Insecticide Resistance ◽

Plutella Xylostella ◽

Diamondback Moth

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Genome-wide identification of long non-coding RNA genes and their association with insecticide resistance and metamorphosis in diamondback moth, Plutella xylostella

Scientific Reports ◽

10.1038/s41598-017-16057-2 ◽

2017 ◽

Vol 7 (1) ◽

Cited By ~ 10

Author(s):

Feiling Liu ◽

Dianhao Guo ◽

Zhuting Yuan ◽

Chen Chen ◽

Huamei Xiao

Keyword(s):

Insecticide Resistance ◽

Plutella Xylostella ◽

Diamondback Moth ◽

Non Coding Rna ◽

Genome Wide ◽

Long Non Coding Rna ◽

Rna Genes

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DNA Sequencing Reveals the Midgut Microbiota of Diamondback Moth, Plutella xylostella (L.) and a Possible Relationship with Insecticide Resistance

PLoS ONE ◽

10.1371/journal.pone.0068852 ◽

2013 ◽

Vol 8 (7) ◽

pp. e68852 ◽

Cited By ~ 62

Author(s):

Xiaofeng Xia ◽

Dandan Zheng ◽

Huanzi Zhong ◽

Bingcai Qin ◽

Geoff M. Gurr ◽

...

Keyword(s):

Dna Sequencing ◽

Insecticide Resistance ◽

Plutella Xylostella ◽

Diamondback Moth

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Genome-wide SNP discovery in field and laboratory colonies of Australian Plutella species

10.1101/141606 ◽

2017 ◽

Cited By ~ 6

Author(s):

K.D. Perry ◽

S.M. Pederson ◽

S.W. Baxter

Keyword(s):

Population Structure ◽

Insecticide Resistance ◽

Population Genetic ◽

Diamondback Moth ◽

Snp Markers ◽

Pest Species ◽

Genetic Studies ◽

High Dispersal ◽

Wild Host ◽

Wide Range

ABSTRACTUnderstanding dispersal and gene flow is an important focus of evolutionary biology, conservation biology and pest management. The diamondback moth, Plutella xylostella, is a worldwide pest of Brassica vegetable and oilseed cropping systems. This insect has high dispersal ability, which has important consequences for population dynamics and the potential spread of insecticide resistance genes. Population genetic studies of the diamondback moth have found little evidence of population structure, suggesting that frequent intermixing occurs within regions, however the patterns of local and regional dispersal remain to be identified. For this and many other pest species, understanding dispersal is crucial for developing integrated management tactics such as forecasting systems and insecticide resistance management plans. In recent years, next generation sequencing (NGS) methods have provided previously unparalleled resolution for population genetic studies in a wide range of species. Here, we assessed the potential of NGS-derived molecular markers to provide new insights about population structure in the diamondback moth. We use restriction-site-associated DNA sequencing (RAD-Seq) to discover hundreds to thousands of single nucleotide polymorphism (SNP) markers in nine field and laboratory-reared populations collected from Australia. Genotypic data from RAD-Seq markers identified a cryptic species, P. australiana, among individuals collected from a wild host, Diplotaxis sp., indicating strong divergence in the nuclear genomes of two Australian Plutella lineages. Significant genetic differentiation was detected among populations of P. xylostella used in our study, however this could be explained by reduced heterozogosity and genetic drift in laboratory-reared populations founded by relatively few individuals. This study demonstrates that RAD-Seq is a powerful method for generating SNP markers for population genetic studies in this species.

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