scholarly journals Horizontal Transmission of the Entomopathogenic Fungal Isolate INRS-242 of Beauveria bassiana (Hypocreales: Cordycipitaceae) in Emerald Ash Borer, Agrilus planipennis (Coleoptera: Buprestidae)

2019 ◽  
Author(s):  
Narin Srei ◽  
Robert Lavallée ◽  
Claude Guertin
Keyword(s):  
Beauveria Bassiana ◽  
Fungal Pathogen ◽  
Biological Control Agent ◽  
Horizontal Transmission ◽  
Emerald Ash Borer ◽  
Control Agent ◽  
Agrilus Planipennis ◽  
Fungal Isolate ◽  
Ash Trees ◽  
Fungus Beauveria Bassiana

Abstract Emerald ash borer (EAB), Agrilus planipennis Fairmaire, is an invasive and destructive beetle that causes extensive damage to ash trees in North America. The entomopathogenic fungus Beauveria bassiana Vuillemin is considered as an effective biological control agent for EAB adult populations. Using an autodissemination device with a fungal isolate of B. bassiana, our research aims to investigate the possibility of horizontal transmission of the fungal disease from infected to uninfected EAB adults during mating. Results show that the efficiency of fungal transmission is significantly related to the sex of EAB carrying the fungal pathogen. EAB males are the promising vector to transmit mycosis to their partners during mating. Results strengthen the potential of the fungal autodissemination device as a powerful biological strategy to control EAB populations.

scholarly journals Horizontal transmission of the entomopathogenic fungal isolate INRS-242 of Beauveria bassiana in emerald ash borer, Agrilus planipennis Fairmaire

2019 ◽  
Author(s):  
Narin Srei ◽  
Robert Lavallée ◽  
Claude Guertin
Keyword(s):  
Biological Control ◽  
North America ◽  
Beauveria Bassiana ◽  
Biological Control Agent ◽  
Horizontal Transmission ◽  
Emerald Ash Borer ◽  
Control Agent ◽  
Agrilus Planipennis ◽  
Fungal Isolate ◽  
Ash Trees

AbstractEmerald ash borer (EAB), Agrilus planipennis Fairmaire, is an invasive and destructive beetle that causes extensive damages to ash trees in North America. The entomopathogenic fungus Beauveria bassiana is considered as an effective biological control agent for EAB adult populations. Using an assisted autodissemination with the fungal isolate INRS-242 of B. bassiana, our research aims to investigate the possibility of horizontal transmission of the fungal disease from infected to uninfected EAB adults during mating. Results show that the efficiency of fungal transmission is significantly related to the sex of EAB carrying the pathogen. EAB males are the promising vector to transmit INRS-242 isolate of B. bassiana to their partners during mating. Results strengthen the potential of the fungal autodissemination device as a powerful biological strategy to control EAB populations.

scholarly journals Evaluation of the fungus Beauveria bassiana (Deuteromycotina: Hyphomycetes), a potential biological control agent of Lutzomyia longipalpis (Diptera, Psychodidae)

2009 ◽  
Vol 50 (3) ◽  
pp. 329-335 ◽  
Author(s):  
Sthenia Santos Albano Amóra ◽  
Claudia Maria Leal Bevilaqua ◽  
Francisco Marlon Carneiro Feijó ◽  
Mariana Araújo Silva ◽  
Romeika Hermínia Macedo Assunção Pereira ◽  
...  
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Biological Control Agent ◽  
Control Agent ◽  
Lutzomyia Longipalpis ◽  
Fungus Beauveria Bassiana

scholarly journals Taxonomic Resolution of the Nematophagous Fungal Isolate ARF18 via Genome Sequencing

2017 ◽  
Vol 5 (34) ◽  
Author(s):  
Sandeep Sharma ◽  
Alex Z. Zaccaron ◽  
John B. Ridenour ◽  
Amy Bradshaw ◽  
Terry L. Kirkpatrick ◽  
...  
Keyword(s):  
Soybean Cyst Nematode ◽  
Heterodera Glycines ◽  
Novel Species ◽  
Biological Control Agent ◽  
Draft Genome ◽  
Control Agent ◽  
Nematophagous Fungus ◽  
Cyst Nematode ◽  
Fungal Isolate ◽  
Taxonomic Resolution

ABSTRACT The taxonomically uncharacterized nematophagous fungus ARF18, which parasitizes cysts, juveniles, and adults of the soybean cyst nematode (Heterodera glycines), was proposed as a nematode biological control agent in 1991. A 46.3-Mb draft genome sequence of this fungus is presented, and a tentative taxonomic identification as a novel species of Brachyphoris is proposed.

Progress in the use of detection dogs for emerald ash borer monitoring

2020 ◽  
Vol 93 (2) ◽  
pp. 326-330 ◽  
Author(s):  
Ute Hoyer-Tomiczek ◽  
Gernot Hoch
Keyword(s):  
Urban Forest ◽  
Emerald Ash Borer ◽  
Initial Study ◽  
Agrilus Planipennis ◽  
Test Series ◽  
Detection Accuracy ◽  
Entry Points ◽  
Ash Trees ◽  
Scent Detection ◽  
Single Blind

Abstract Early detection of infestation by the emerald ash borer (EAB), Agrilus planipennis is extremely difficult; hence developing additional methods is desirable. We built on the successful use of canine scent detection for the invasive long-horned beetles Anoplophora glabripennis and Anoplophora chinensis and trained six dogs in detection of EAB. A first test series was performed to evaluate detection accuracy of five of these dogs. Seven different experimental settings were tested under single blind conditions: (1) forest nursery, (2) piles of firewood, (3) firewood on the ground, (4) ash logs on the ground, (5) old urban ash trees, (6) urban forest with ash trees and (7) natural forest with ash trees. In total, 214 positive samples were presented to the dogs, out of which 20 remained undetected. The experiments ascertained sensitivity (correct positives of all positives) ranging from 73.3 to 100 percent and specificity (correct negatives of all negatives) from 88.9 to 99.8 percent in the tested settings. This initial study demonstrates that trained dogs are able to detect EAB scent from sources such as larval galleries in bark/wood, frass, living or dead larvae or dead dry beetles. The numbers of tested dogs and test series were limited, and further studies are needed to confirm the initial results. However, the preliminary findings demonstrate the potential of the method particularly for inspection of wood or plants at entry points.

scholarly journals Existence of entomopathogen fungi, Beauveria bassiana as an endophyte in cacao seedlings

2015 ◽  
Vol 31 (3) ◽  
pp. 186-195
Author(s):  
Endang Sulistyowati ◽  
Febrilia Nur AINI
Keyword(s):  
Beauveria Bassiana ◽  
Plant Protection ◽  
Biological Control Agent ◽  
Block Design ◽  
Control Agent ◽  
Post Inoculation ◽  
Pod Borer ◽  
Fungal Entomopathogens ◽  
Randomized Complete Block Design ◽  
Fungal Entomopathogen

Beauveria bassiana is one of the entomopathogen fungi which is known as biological control agent of cocoa pod borer and cocoa mirids (Helopeltis spp.). Because of its effectiveness in the fields is still not consistent, so we conduct a research with the objective to know the possibility of Beauveria bassiana to be established as a endophyte. Various fungal entomopathogens have already been reported as endophytes and the various methods used to inoculate the plants with B. bassiana were partially effective. The research has been conducted in laboratory of Plant Protection, Indonesian Coffee and Cocoa Research Institute by inoculating of cocoa seeds and cocoa nursery with B. bassiana suspension.  The trial was arranged  by randomized complete block design with a factorial arrangement. The factor were spore concentration of B. bassiana (0; 2; and 4 g/ 10 l) and cocoa varieties (family of ICS 60, TSH858, and hybrid). The trial were use  four replications. The results showed that the fungal entomopathogen B. bassiana was established as an endophyte in cocoa seedling, both from cocoa seeds and nursery application. Percentage of existence of B. bassiana colonies as endophytes one month after seeds application were ICS 60 amounted to 93.3 % both on concentration treatments, while the families of TSH 858 by 80 % and 86.67 % respectively in 2 g and 4 g per 10 l of B. bassiana spores concentration treament.. The lowest percentage was in hybrids, which amounted to 66.67% and 50%. B. bassiana colonies was exixtence as an endophyte in culture from root, stem and leaves of cocoa seedling up to 5 months post inoculation. While the application on nursery by soil drenshing, leaf spraying, and stem injection , it was known that B. bassiana colonies were found in the tissues of leaves, stems, and roots until two months after application. Colonies of B. bassiana as endophytes still exsist until six weeks after nursery was planted in the field. 

scholarly journals Evaluation of Xylem Discoloration in Ash Trees Associated with Macroinjections of a Systemic Insecticide

2016 ◽  
Vol 42 (6) ◽  
Author(s):  
Sara Tanis ◽  
Deborah McCullough
Keyword(s):  
Laser Scanning ◽  
Emerald Ash Borer ◽  
Agrilus Planipennis ◽  
High Rate ◽  
Confocal Laser ◽  
Green Ash ◽  
Pathogen Infection ◽  
White Ash ◽  
Ash Trees ◽  
Canadian Provinces

Emerald ash borer (EAB) (Agrilus planipennis), first identified near Detroit, Michigan, U.S., in 2002, has killed millions of ash trees (Fraxinus spp.) in 28 states and two Canadian provinces to date. Trunk injections of insecticide products containing emamectin benzoate (EB) (e.g., TREE-ageR) are often used to protect ash trees in landscapes from EAB, but wounds and potential injury resulting from injections are a concern. Researchers examined 507 injection sites on 61 trees and recorded evidence of secondary wounding (e.g., external bark cracks, internal xylem necrosis and pathogen infection). Researchers assessed 233 injection sites on 22 green ash and 24 white ash trees macro-injected with a low or a medium-high rate of EB in 2008 only, or in both 2008 and 2009. Only 12 of 233 injection sites (5%) were associated with external bark cracks and there was no evidence of pathogen infection. On 39 of the 46 trees (85%), new xylem was growing over injection sites. Researchers assessed 274 injection sites on 15 green ash trees injected annually with EB from 2008 to 2013 or injected in 2008 and again in 2011. Bark cracks were associated with four injection sites on three trees, but no evidence of injury was found on the other 12 trees. All 15 trees had new xylem laid over injection sites. Confocal laser scanning and polarizing digital microscopy were used to assess the integrity of discolored xylem tissue removed from the immediate area surrounding 140 injection sites on 61 trees. Researchers found no evidence of decay associated with discoloration.

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