Previous research has documented low frequencies of interspecific mating in nature between the invasive vectors
Aedes aegypti
and
Aedes albopictus
. It is also known that heterospecific male accessory gland substances transferred during mating sterilize
A. aegypti
but not
A. albopictus
females, leading to satyrization, a form of reproductive interference. This paper demonstrates that satyrization of
A. aegypti
by
A. albopictus
may occur without evidence of successful insemination. Our results show that
A. aegypti
females, previously exposed to
A. albopictus
males, are rendered refractory to subsequent conspecific mating even though their spermathecae contain no heterospecific sperm. Additional experiments demonstrating transfer of labelled semen from
A. albopictus
males to
A. aegypti
females and low production of viable eggs of females housed with conspecific males, following exposure to
A. albopictus
males, confirm higher incidences of satyrization than expected, based on heterospecific insemination rates. We conclude that frequencies of satyrization based on detection of interspecific sperm in spermathecae may underestimate the impact of this form of reproductive interference.