scholarly journals Ultrastructural Comparison of External Morphology of Immature Stages of Lutzomyia (Nyssomyia) intermedia and Lutzomyia (Nyssomyia) whitmani (Diptera: Psychodidae), Vectors of Cutaneous Leishmaniasis, by Scanning Electron Microscopy

2007 ◽  
Vol 44 (6) ◽  
pp. 903-914 ◽  
Author(s):  
Ana C. Bahia ◽  
Nágila F. C. Secundino ◽  
José C. Miranda ◽  
Deboraci B. Prates ◽  
Ana P. A. Souza ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cutaneous Leishmaniasis ◽  
External Morphology ◽  
Immature Stages ◽  
Scanning Electron

Description of immatures of Galeacius martini Schouteden (Hemiptera: Heteroptera: Scutelleridae)

Zootaxa ◽  
2011 ◽  
Vol 3110 (1) ◽  
pp. 37 ◽  
Author(s):  
FILIPE M. BIANCHI ◽  
THEREZA A. GARBELOTTO ◽  
LUIZ A. CAMPOS
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
External Morphology ◽  
Immature Stages ◽  
Efferent System ◽  
Scanning Electron

Scutelleridae occur worldwide, and immature stages have been scarcely studied. Galeacius Distant is a Neotropical genus currently containing four species. Here the external morphology of immatures of Galeacius martini Schouteden is described. Adults and nymphs were maintained in laboratory and fed on branches of Miconia sellowiana with fruits. The egg of G. martini is semi-spherical, reddish brown, with reticulations surrounding smaller granulated sculpturing under scanning electron microscopy (S.E.M). The nymphs from first to fifth instar have a stridulitrum plus plectrum, and 2+2 trichobothria posterior to spiracles on abdominal sternites III–VII. The dorso-abdominal scent efferent system has an evaporatorium with two distinct mycoid sculpturing, and their are two types of peritreme on medial plates II and III in first and fifth instar. The color and chorion morphology may distinguish G. martini from other scutellerid eggs so far described. New descriptions of immatures of Scutelleridae are particularly desirable, mainly with emphasis some structures on S.E.M., as stridulatory devices, dorso-abdominal scent efferent system, and trichobothria, thus improving the knowledge about the taxon.

External morphology of immature of Tynacantha marginata Dallas, 1851 (Hemiptera, Heteroptera, Pentatomidae)

Zootaxa ◽  
2018 ◽  
Vol 4378 (1) ◽  
pp. 121 ◽  
Author(s):  
RICARDO BRUGNERA ◽  
JOCÉLIA GRAZIA
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Early Identification ◽  
Smooth Surface ◽  
External Morphology ◽  
Immature Stages ◽  
First Instar ◽  
Lateral Margins ◽  
Heteroptera Pentatomidae ◽  
Scanning Electron

Immature stages of Neotropical Asopinae have been poorly studied. Here, the external morphology of eggs and nymphs of Tynacantha marginata Dallas are described using light and scanning electron microscopy. The egg is barrel-shaped with smooth surface, and long aero-micropylar processes. In the first instar, the color of abdomen varies along the development from yellow to red, the dorsal plates are pale, and the surface of evaporatorium bears spiked projections. From the second, the evaporatorium surface is reticulated. From third instar, the pronotum is orange with black lateral margins. The eggs and nymphs of T. marginata are unique among Neotropical Asopinae species of which the morphology is known, allowing the early identification of the species. 

scholarly journals External morphology of the immature stages of Neotropical heliconians: X. Heliconius sara apseudes (Lepidoptera, Nymphalidae, Heliconiinae)

2015 ◽  
Vol 105 (4) ◽  
pp. 523-533
Author(s):  
Kim R. Barão ◽  
Denis S. Silva ◽  
Gilson R. P. Moreira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Instar Larva ◽  
External Morphology ◽  
Immature Stages ◽  
First Instar ◽  
Scanning Electron

ABSTRACT The biology and morphology of the immature stages of Heliconius sara apseudes (Hübner, [1813]) are still little known. External features of the egg, larvae and pupa of H. sara apseudes are described and illustrated, based upon light and scanning electron microscopy. Eggs with smooth carina, first instar larva with scaly setae, and body of second to fifth instars covered with scattered pinnacles distinguish H. sara apseudes from other heliconiine species.

The External Morphology of the Eggs of Culex (Culex) saltanensis (Diptera: Culicidae) Under Scanning Electron Microscopy

2020 ◽  
Author(s):  
J R Santos-Mallet ◽  
T D Balthazar ◽  
A A Oliveira ◽  
W A Marques ◽  
A Q Bastos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Optical Microscopy ◽  
Osmium Tetroxide ◽  
Neotropical Region ◽  
External Morphology ◽  
Metal Supports ◽  
Scanning Electron

Abstract The aim of the present study was to describe the morphology of the eggs of Culex (Culex) saltanensis Dyar that occurs in the Neotropical region. Eggs of the Cx. (Cux.) saltanensis were collected at the Mata Atlântica FIOCRUZ campus, fixed in 1% osmium tetroxide, prepared for mounting on metal supports, observed under a scanning electron microscope, and described morphologically. The eggs had a coniform shape with a length of approximately 0.5 mm (505–510 µm) and a width in the median portion of 117 µm (113–123 µm). Upper portion is lined with tubers of irregular shape and varying sizes (0.64–1.31 µm), located on a cross-linked matrix forming bands observed under optical microscopy. The micropyle is encased in a necklace of approximately 6.6-µm plates arranged in a flower-like shape. Comparing Cx. (Cux.) saltanensis eggs with several species of different genera, important divergent characteristics can be observed. However, this study points to the need for new descriptions of eggs of species belonging to the same subgenus in order to analyze if there will be differences between them. Culex (Cux.) saltanensis eggs have particular characteristics not observed in eggs of other Culicidae genera.

Antennal Sensilla of Chrysis shanghalensis (Hymenoptera: Chrysididae), a Larval Ectoparasite of Monema flavescens (Lepidoptera: Limacodidae)

2021 ◽  
Vol 56 (1) ◽  
pp. 1-11
Author(s):  
Shu Z. Yang ◽  
Mei H. Yang ◽  
Yun Xu ◽  
Jin T. Zhang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Natural Enemy ◽  
Tree Species ◽  
External Morphology ◽  
Antennal Sensilla ◽  
Sensilla Basiconica ◽  
Sensilla Trichodea ◽  
Males And Females ◽  
Scanning Electron

Abstract Chrysis shanghalensis Smith (Hymenoptera: Chrysididae) is an ectoparasitoid and important natural enemy of Monema flavescens Walker (Lepidoptera: Limacodidae), a serious defoliator of a number of tree species. The external morphology of the antennal sensilla of this parasitoid was examined by scanning electron microscopy, and types and distribution of sensilla were recorded. The antennae of C. shanghalensis were geniculate in shape and composed of a scape with radicula, a pedicel, and a flagellum divided into 11 flagellomeres in males and females. Cuticular pore and 14 types of sensilla were identified on the antennae of both sexes. These included aporous Böhm's bristles, sensilla trichodea 1, 2, and 3 (nonporous), sensilla trichodea 4 (multiporous), two types of sensilla chaetica (nonporous), four morphological types of sensilla basiconica (multiporous), two morphological types of sensilla coeloconica (multiporous), and one type of sensillum campaniforme (nonporous).

Comparative morphology of myrmecophilous immature stages of European Microdon species (Diptera: Syrphidae): updated identification key and new diagnostic characters

Zootaxa ◽  
2020 ◽  
Vol 4789 (2) ◽  
pp. 348-370 ◽  
Author(s):  
GIULIA SCARPARO ◽  
ROBERT WOLTON ◽  
MARCO MOLFINI ◽  
LUIGI CAO PINNA ◽  
ANDREA DI GIULIO
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sibling Species ◽  
Comparative Morphology ◽  
Immature Stages ◽  
Outward Appearance ◽  
Diagnostic Features ◽  
Diagnostic Characters ◽  
Ant Nests ◽  
Scanning Electron

Hoverflies (Diptera: Syrphidae) of the genus Microdon Meigen have larvae that live in ant nests where they are predatory on ant larvae. Reflecting the exceptional challenges of this very specialized lifestyle, Microdon eggs, larvae and puparia are highly distinctive in their morphology. Detailed descriptions of these immature stages is, however, lacking for all but a very few species, and much of this has been limited through the sole use of light microscopes. Here, using Scanning Electron Microscopy (SEM), we present detailed, comparative descriptions of the immature stages of three European Microdon species: M. analis, M. devius and M. myrmicae. Given that many adult Microdon species are very similar to each other in their outward appearance, we demonstrate that the morphology of their immature stages can improve our understanding of the phylogeny of the genus. We also discuss how particular adaptations of the immature morphology may allow their myrmecophilous life within ant nests. In this paper new diagnostic features are also presented to distinguish M. myrmicae from its sibling species M. mutabilis—the two are morphologically indistinguishable as adults. 

Anatomical characterisation of the foliar colleters in Myrtoideae (Myrtaceae)

2012 ◽  
Vol 60 (8) ◽  
pp. 707 ◽  
Author(s):  
Cleber J. da Silva ◽  
Luiz Cláudio de A. Barbosa ◽  
Ana E. Marques ◽  
Maria Cristina Baracat-Pereira ◽  
Antônio L. Pinheiro ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Phylogenetic Relationships ◽  
External Morphology ◽  
Secretory Structures ◽  
Reproductive Shoot ◽  
Sds Page ◽  
Histochemical Tests ◽  
Five Tribes ◽  
Scanning Electron

Colleters are secretory structures that occur in vegetative or reproductive shoot apices of many botanical families. However, in the order Myrtales, reports of colleters have considered only external morphology. We therefore evaluated apical meristems of 52 species belonging to 17 genera from seven tribes of subfamily Myrtoideae (Myrtaceae), so as to analyse the incidence and morphological types of colleters. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on fresh and methacrylate-embedded material. Proteins of the colleter secretions were analysed by SDS-PAGE. We have classified and described the following three new colleter types: petaloid, conic and euryform. None of the species contained all three colleter types. The petaloid colleters were present in three tribes (Syzygieae, Melaleuceae and Lophostemoneae). The conic colleters were observed in three tribes (Leptospermeae, Myrteae and Melaleuceae) and the euryform type occurred in five tribes (Leptospermeae, Syncarpieae, Myrteae, Syzygieae and Melaleuceae). In the tribe Eucalypteae, we found no evidence of colleters. The presence of mucilaginous secretion that defines colleters was confirmed by histochemical tests, and no proteins were found in the secretion. The colleters in Myrtoideae may help clarify the phylogenetic relationships of the Myrtaceae family.

scholarly journals Morphological aspects of immature stages of Migonemyia migonei (Diptera: Psychodidae, Phlebotominae), an important vector of Leishmaniosis in South America, described by scanning electron microscopy

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242163
Author(s):  
Eric Fabrício Marialva ◽  
Nágila F. Secundino ◽  
Fernando F. Fernandes ◽  
Helena R. C. Araújo ◽  
Claudia M. Ríos-Velásquez ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
South America ◽  
Leishmania Infantum ◽  
Immature Stages ◽  
Vector Species ◽  
Future Studies ◽  
Larval Stages ◽  
Morphological Aspects ◽  
Scanning Electron

We describe the immature stages of Migonemyia migonei, which is the vector of Leishmania (Viannia) braziliensis, the etiological agent of cutaneous leishmaniasis in South America, and a putative vector of Leishmania infantum chagasi. Scanning Electron Microscopy (SEM) was used to refine the description of the structures of the egg, all instar larvae, and the pupa. The eggs have polygonal cells on the egg exochorion, and differences between larval and pupal chaetotaxy have been highlighted. Different sensillary subtypes—trichoidea, basiconica, coelonica and campanoformia—were observed in the larval stages. The results presented herein contribute to the taxonomy of Mg. migonei and may contribute to future studies on the phylogeny of this important vector species.

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