Fine structure of Aldrovanda vesiculosa L: the peculiar lifestyle of an aquatic carnivorous plant elucidated by electron microscopy using cryo-techniques

Microscopy ◽  
2020 ◽  
Vol 69 (4) ◽  
pp. 214-226 ◽  
Author(s):  
Kimie Atsuzawa ◽  
Daiki Kanaizumi ◽  
Mizuki Ajisaka ◽  
Tasuku Kamada ◽  
Kimie Sakamoto ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Culture System ◽  
Digestive Enzymes ◽  
Natural Habitat ◽  
Electron Microscopic Observation ◽  
Carnivorous Plant ◽  
Way Of Life ◽  
Electron Microscopic ◽  
Digestive Glands

Abstract The aquatic carnivorous plant Aldrovanda vesiculosa L. is critically endangered worldwide; its peculiar lifestyle raises many questions and poses problems both intriguing on their own and relevant to conservation. While establishing a culture system for its propagation and restoring its natural habitat in Hozoji pond in Saitama, Japan, we conducted ultrastructural observations to examine the various aspects of Aldrovanda’s way of life. Electron microscopic observation in combination with cryo-techniques produced novel information which could not be obtained by other methods. Some of the results are: phosphorous is stored in petiole cells of turions during winter; mucilaginous guides are provided for pollen tubes in parietal placental ovaries; storage of potassium in the vicinity of the midrib of carnivorous leaves may contribute to the rapid closing of the carnivorous leaves; dynamic sequential changes of the ultrastructure of digestive glands are involved in the synthesis and secretion of digestive enzymes, including protease and acid phosphatase. These results should contribute significantly to our understanding of Aldrovanda and the detailed mechanisms of its life.

Electron Microscopic Observation of the Longitudinal Organization of Poly (p-Phenylene Terephthalamide) Fibers

1982 ◽  
Vol 40 ◽  
pp. 680-681
Author(s):  
Li Li-Sheng ◽  
L.F. Allard ◽  
W.C. Bigelow
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Aromatic Polyamides ◽  
Radial Arrangement ◽  
Scanning Electron ◽  
Better Than

The aromatic polyamides form a class of fibers having mechanical properties which are much better than those of aliphatic polyamides. Currently, the accepted morphology of these fibers as proposed by M.G. Dobb, et al. is a radial arrangement of pleated sheets, with the plane of the pleats parallel to the axis of the fiber. We have recently obtained evidence which supports a different morphology of this type of fiber, using ultramicrotomy and ion-thinning techniques to prepare specimens for transmission and scanning electron microscopy.

PB-06Improved Method for Electron Microscopic Observation of Fine Structure of Yeasts Engulfed by Cultured Macrophages

Microscopy ◽  
2016 ◽  
Vol 65 (suppl 1) ◽  
pp. i26.2-i26
Author(s):  
Masashi Yamaguchi ◽  
Azusa Takahashi ◽  
Yuko Aida ◽  
Hiroji Chibana
Keyword(s):  
Fine Structure ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic

scholarly journals Rhabdias bufonis (Rhabdiasidae) from the lung of the African common toad, Amietophrynus regularis (Bufonidae) in Egypt. New data on the basis of light and scanning electron microscopic study

2018 ◽  
Author(s):  
Kareem Morsy ◽  
Sara Ali ◽  
Fathy Abdel Ghaffar ◽  
Hoda El Fayoumi ◽  
Heba Abdel Haleem
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Natural Habitat ◽  
Mouth Opening ◽  
Tail Length ◽  
Buccal Capsule ◽  
Common Toad ◽  
Electron Microscopic ◽  
Oral Opening ◽  
Scanning Electron

Background and Aims: Rhabdias bufonis (Rhabdiasidae) is a nematode parasite of family Rhabdiasidae infecting the lung of amphibians. The present study provides new morphological details for R. bufonis isolated from the lungs of the African common toad, Amietophrynus regularis based on observations of light and scanning electron microscopy. Methods: Forty specimens were collected during the period from March to August 2017 from its natural habitat; the damp, moist fields and gardens at Giza governorate, Egypt. Nematode worms were isolated from the lungs, fixed and then preserved. They were examined using light and scanning electron microscopy with further line drawings. Results: 14 specimens (35%) were found to harbor a large number of Rhabdias. Body of the gravid females were elongated, slender, measured 3.22–9.86 (5.64±0.03) long and 0.09–0.48 (0.23±0.02) mm wide at mid–length. Buccal capsule was with cylindrical lumen and sclerotised walls 13–31 (18 ± 2) long and 16–27 (24 ± 2) mm wide. Ovaries were almost straight. The uteri were located anterior and posterior to the vulva. Uterus filled with large number of eggs (17-42). Eggs were oval in shape, 0.120–0.132 (0.126±0.002) × 0.039–0.812 (0.517±0.02) mm and some of them were with fully developed larvae inside. The tail was comparatively short, gradually tapered, tail length 0.131–0.435 (0.320±0.02) mm long. SEM showed that worms possessed an oval anterior end with a simple, slit like oral opening. The lipless edges of mouth opening bordered with tiny cuticular elevations, radiated outwards. Two pairs of submedian cephalic papillae were observed around the oral opening as well as two amphids. Conclusion: The current study introduced new morphological details for R. bufonis isolated from the Egyptian frog, which was not reported in the previous studies, of them, the morphology of the slit–like mouth opening, the two pairs of cephalic papillae, two amphids and the three pairs of cuticular elevations supporting the area around mouth opening.

An electron microscopic study of Amoeba proteus

1959 ◽  
Vol 150 (939) ◽  
pp. 216-232 ◽  
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Amoeba Proteus ◽  
Contractile Vacuole ◽  
Electron Microscopic ◽  
Functional Implications ◽  
Food Vacuoles

Methods of fixing, embedding and sectioning of Amoeba proteus for electron microscopy are described. The fine structure of the various organelles: nucleus, mitochondria, food vacuoles; and the contractile vacuole, is discussed in detail. A number of minor unidentified objects has also been found. The functional implications of the structural findings are considered.

scholarly journals THE FINE STRUCTURE OF BOVINE NASAL CARTILAGE

1971 ◽  
Vol 49 (3) ◽  
pp. 650-663 ◽  
Author(s):  
H. Clarke Anderson ◽  
Stanley W. Sajdera
Keyword(s):  
Electron Microscopy ◽  
Fine Structure ◽  
Banding Pattern ◽  
Collagen Fibrils ◽  
Guanidinium Chloride ◽  
Electron Microscopic ◽  
Nasal Cartilage ◽  
Before And After ◽  
Exhaustive Extraction

Bovine nasal cartilage was studied by electron microscopy before and after extraction with 4 M guanidinium chloride or 1.9 M CaCl2. These solvents removed matrix granules, basophilia, and 85% of the proteoglycan complex, measured as hexuronate. Simultaneously, many collagen fibrils were disaggregated into component microfibrils (approximately 40 A thick). In contrast to the above solvents, exhaustive extraction with 0.5 M guanidinium chloride removed 20% of the proteoglycan complex, and matrix granules were reduced in size but not in number. Extraction with 4 M CaCl2 removed only 10% of the proteoglycan complex, did not remove matrix granules, and caused the normal banding pattern of collagen to disappear. The banding was restored by further treatment with trypsin. Trypsin, before or after 4 M CaCl2, removed matrix granules and 90% of the proteoglycan complex. We conclude that matrix granules are an electron microscopic representation of the proteoglycan complex, and consist of more than one proteoglycan macromolecule. It would appear that 4 M guanidinium chloride and 1.9 M CaCl2, in addition to removing most of the proteoglycan complex, also disaggregate some of the collagen fibrils into their component microfibrils.

scholarly journals Rhabdias bufonis (Rhabdiasidae) from the lung of the African common toad, Amietophrynus regularis (Bufonidae) in Egypt. New data on the basis of light and scanning electron microscopic study

2018 ◽  
Author(s):  
Kareem Morsy ◽  
Sara Ali ◽  
Fathy Abdel Ghaffar ◽  
Hoda El Fayoumi ◽  
Heba Abdel Haleem
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Natural Habitat ◽  
Mouth Opening ◽  
Tail Length ◽  
Buccal Capsule ◽  
Common Toad ◽  
Electron Microscopic ◽  
Oral Opening ◽  
Scanning Electron

Background and Aims: Rhabdias bufonis (Rhabdiasidae) is a nematode parasite of family Rhabdiasidae infecting the lung of amphibians. The present study provides new morphological details for R. bufonis isolated from the lungs of the African common toad, Amietophrynus regularis based on observations of light and scanning electron microscopy. Methods: Forty specimens were collected during the period from March to August 2017 from its natural habitat; the damp, moist fields and gardens at Giza governorate, Egypt. Nematode worms were isolated from the lungs, fixed and then preserved. They were examined using light and scanning electron microscopy with further line drawings. Results: 14 specimens (35%) were found to harbor a large number of Rhabdias. Body of the gravid females were elongated, slender, measured 3.22–9.86 (5.64±0.03) long and 0.09–0.48 (0.23±0.02) mm wide at mid–length. Buccal capsule was with cylindrical lumen and sclerotised walls 13–31 (18 ± 2) long and 16–27 (24 ± 2) mm wide. Ovaries were almost straight. The uteri were located anterior and posterior to the vulva. Uterus filled with large number of eggs (17-42). Eggs were oval in shape, 0.120–0.132 (0.126±0.002) × 0.039–0.812 (0.517±0.02) mm and some of them were with fully developed larvae inside. The tail was comparatively short, gradually tapered, tail length 0.131–0.435 (0.320±0.02) mm long. SEM showed that worms possessed an oval anterior end with a simple, slit like oral opening. The lipless edges of mouth opening bordered with tiny cuticular elevations, radiated outwards. Two pairs of submedian cephalic papillae were observed around the oral opening as well as two amphids. Conclusion: The current study introduced new morphological details for R. bufonis isolated from the Egyptian frog, which was not reported in the previous studies, of them, the morphology of the slit–like mouth opening, the two pairs of cephalic papillae, two amphids and the three pairs of cuticular elevations supporting the area around mouth opening.

Electron Microscopic observation on the semen of HIV-positive men

1990 ◽  
Vol 48 (3) ◽  
pp. 348-349
Author(s):  
James K. Koehler ◽  
Robert W. Coombs ◽  
Ann C. Collier ◽  
John N. Krieger ◽  
Lawrence Corey
Keyword(s):  
Electron Microscopy ◽  
Peripheral Blood ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Zidovudine Therapy ◽  
Hiv Positive ◽  
Disease Category ◽  
Electron Microscopic ◽  
Free Plasma ◽  
Culture Positive

Despite well-documented studies showing HIV transmission by and isolation from semen, relatively little information is available concerning the morphology and cellular characteristics of the semen of HIV positive individuals. We have there fore examined the semen of HIV infected patients with electron microscopy in order to determine cellular ultrastructure and possible sites of virus in this material. Patients were assayed for HIV in peripheral blood, and cell free plasma, as well as semen and CD-4-T-cells were counted. Ejaculates were obtained from 9 HIV positive individuals. Five had symptomatic HIV infections: three had AIDS and were on zidovudine therapy, two had ARC (AIDS Related Complex); four were asymptomatic. All were HIV culture positive from blood, and two were HIV culture positive from semen. Table 1 details the disease category and HIV assay data in these patients. Electron microscopy revealed that semen from the symptomatic AIDS group was infiltrated with macrophages and other white cells with extensive phagocytosis of sperm and other debris (Figure 1).

Light and Electron Microscopic Observation of Regenerated Fungiform Taste Buds in Patients with Recovered Taste Function after Severing Chorda Tympani Nerve

2011 ◽  
Vol 120 (11) ◽  
pp. 713-721 ◽  
Author(s):  
Takehisa Saito ◽  
Tetsufumi Ito ◽  
Norihiko Narita ◽  
Takechiyo Yamada ◽  
Yasuhiro Manabe
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Taste Buds ◽  
Chorda Tympani ◽  
Chorda Tympani Nerve ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
The Mean

Objectives: The aim of this study was to evaluate the mean number of regenerated fungiform taste buds per papilla and perform light and electron microscopic observation of taste buds in patients with recovered taste function after severing the chorda tympani nerve during middle ear surgery. Methods: We performed a biopsy on the fungiform papillae (FP) in the midlateral region of the dorsal surface of the tongue from 5 control volunteers (33 total FP) and from 7 and 5 patients with and without taste recovery (34 and 29 FP, respectively) 3 years 6 months to 18 years after surgery. The specimens were observed by light and transmission electron microscopy. The taste function was evaluated by electrogustometry. Results: The mean number of taste buds in the FP of patients with completely recovered taste function was significantly smaller (1.9 ± 1.4 per papilla; p < 0.01) than that of the control subjects (3.8 ± 2.2 per papilla). By transmission electron microscopy, 4 distinct types of cell (type I, II, III, and basal cells) were identified in the regenerated taste buds. Nerve fibers and nerve terminals were also found in the taste buds. Conclusions: It was clarified that taste buds containing taste cells and nerve endings do regenerate in the FP of patients with recovered taste function.

Electron Microscopic Observation of Fine Structure of Cu3Se2 Crystals

1971 ◽  
Vol 42 (5) ◽  
pp. 2143-2145 ◽  
Author(s):  
Hiroshi Morikawa ◽  
Makoto Shiojiri ◽  
Eiji Suito
Keyword(s):  
Fine Structure ◽  
Microscopic Observation ◽  
Electron Microscopic Observation ◽  
Electron Microscopic

Synthesis and Characterization of Hydrotalcite like Compound via a Facile Solid State Method

MRS Advances ◽  
2016 ◽  
Vol 1 (36) ◽  
pp. 2557-2562
Author(s):  
Abbas Fahami ◽  
Gary W. Beall
Keyword(s):  
Electron Microscopy ◽  
Solid State ◽  
Structural Characteristics ◽  
Formation Rate ◽  
Average Particle Size ◽  
Electron Microscopic Observation ◽  
Average Particle ◽  
Electron Microscopic ◽  
Solid State Method ◽  
State Method

ABSTRACTCarbonate intercalated Mg-Al layered double hydroxides (Mg-Al-CO3-LDH) were successfully produced by mechanical alloying process using different starting raw chemicals. Two distinct chemical reactions were activated at different milling times. The samples were characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), and transmission electron microscopy (TEM). Results revealed that the structural characteristics of Mg-Al-CO3-LDH such as lattice parameters, unit cell volume, and the interlayer spacing were affected strongly by milling time. Based on the XRD data, the formation rate of pure Hydrotalcite (HT) was strongly influenced by the chemical composition of raw materials. Electron microscopic observation displayed that the final product had a platelet-like structure with an average particle size of 20-100 nm. Therefore, the synthesis of Mg-Al-CO3-LDH via a cost-effective solid-state method owing to simplicity and reproducibility can be a promising candidate especially for use in biomaterials and catalyst industries.

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