Electron Microscopic observation on the semen of HIV-positive men
Despite well-documented studies showing HIV transmission by and isolation from semen, relatively little information is available concerning the morphology and cellular characteristics of the semen of HIV positive individuals. We have there fore examined the semen of HIV infected patients with electron microscopy in order to determine cellular ultrastructure and possible sites of virus in this material. Patients were assayed for HIV in peripheral blood, and cell free plasma, as well as semen and CD-4-T-cells were counted. Ejaculates were obtained from 9 HIV positive individuals. Five had symptomatic HIV infections: three had AIDS and were on zidovudine therapy, two had ARC (AIDS Related Complex); four were asymptomatic. All were HIV culture positive from blood, and two were HIV culture positive from semen. Table 1 details the disease category and HIV assay data in these patients. Electron microscopy revealed that semen from the symptomatic AIDS group was infiltrated with macrophages and other white cells with extensive phagocytosis of sperm and other debris (Figure 1).