Electron Microscopic observation on the semen of HIV-positive men

Author(s):  
James K. Koehler ◽  
Robert W. Coombs ◽  
Ann C. Collier ◽  
John N. Krieger ◽  
Lawrence Corey

Despite well-documented studies showing HIV transmission by and isolation from semen, relatively little information is available concerning the morphology and cellular characteristics of the semen of HIV positive individuals. We have there fore examined the semen of HIV infected patients with electron microscopy in order to determine cellular ultrastructure and possible sites of virus in this material. Patients were assayed for HIV in peripheral blood, and cell free plasma, as well as semen and CD-4-T-cells were counted. Ejaculates were obtained from 9 HIV positive individuals. Five had symptomatic HIV infections: three had AIDS and were on zidovudine therapy, two had ARC (AIDS Related Complex); four were asymptomatic. All were HIV culture positive from blood, and two were HIV culture positive from semen. Table 1 details the disease category and HIV assay data in these patients. Electron microscopy revealed that semen from the symptomatic AIDS group was infiltrated with macrophages and other white cells with extensive phagocytosis of sperm and other debris (Figure 1).

Author(s):  
Li Li-Sheng ◽  
L.F. Allard ◽  
W.C. Bigelow

The aromatic polyamides form a class of fibers having mechanical properties which are much better than those of aliphatic polyamides. Currently, the accepted morphology of these fibers as proposed by M.G. Dobb, et al. is a radial arrangement of pleated sheets, with the plane of the pleats parallel to the axis of the fiber. We have recently obtained evidence which supports a different morphology of this type of fiber, using ultramicrotomy and ion-thinning techniques to prepare specimens for transmission and scanning electron microscopy.


2011 ◽  
Vol 120 (11) ◽  
pp. 713-721 ◽  
Author(s):  
Takehisa Saito ◽  
Tetsufumi Ito ◽  
Norihiko Narita ◽  
Takechiyo Yamada ◽  
Yasuhiro Manabe

Objectives: The aim of this study was to evaluate the mean number of regenerated fungiform taste buds per papilla and perform light and electron microscopic observation of taste buds in patients with recovered taste function after severing the chorda tympani nerve during middle ear surgery. Methods: We performed a biopsy on the fungiform papillae (FP) in the midlateral region of the dorsal surface of the tongue from 5 control volunteers (33 total FP) and from 7 and 5 patients with and without taste recovery (34 and 29 FP, respectively) 3 years 6 months to 18 years after surgery. The specimens were observed by light and transmission electron microscopy. The taste function was evaluated by electrogustometry. Results: The mean number of taste buds in the FP of patients with completely recovered taste function was significantly smaller (1.9 ± 1.4 per papilla; p < 0.01) than that of the control subjects (3.8 ± 2.2 per papilla). By transmission electron microscopy, 4 distinct types of cell (type I, II, III, and basal cells) were identified in the regenerated taste buds. Nerve fibers and nerve terminals were also found in the taste buds. Conclusions: It was clarified that taste buds containing taste cells and nerve endings do regenerate in the FP of patients with recovered taste function.


1990 ◽  
Vol 213 ◽  
Author(s):  
Seung-Hyuk Kang ◽  
Seung-Jun Jeon ◽  
Hu-Chul Lee

ABSTRACTIn an effort to seek a way to toughen NiAL intermetallic compounds, deformation characteristics of copper containing NiAL martensite were studied by electron microscopy. Martensite plates with fine {111} <112> internal twins were well developed across the grains in as extruded alloys. Initial linear work hardening up to 7 % strain was observed and the migration of twin interface and martensite plate interfaces were suggested as major deformation modes at this stage. Increase in strain hardening rate after 7 % strain was associated with the development of dislocation structure and growth of new strain induced twins within the internal twin free martensite plates.


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