PB-01Reliable Method for Obtaining Serial Ultrathin Sections in Transmission Electron Microscopy

Microscopy ◽  
2017 ◽  
Vol 66 (suppl_1) ◽  
pp. i33-i33
Author(s):  
Masashi Yamaguchi ◽  
Shigeo Kita ◽  
Setsuo Maruta ◽  
Hiroji Chibana
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Serial Ultrathin Sections

Magnesium and sulfur in mast cell and basophil granules of lower vertebrates in relation with histamine

1992 ◽  
Vol 50 (2) ◽  
pp. 1596-1597
Author(s):  
Kenichi Takaya
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Mast Cell ◽  
Mast Cells ◽  
Tree Frog ◽  
X Ray ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Fresh Frozen ◽  
Ultrathin Sections

Mast cell and basophil granules of the vertebrate contain heparin or related sulfated proteoglycans. Histamine is also present in mammalian mast cells and basophils. However, no histamine is detected in mast cell granules of the amphibian or fish, while it is shown in those of reptiles and birds A quantitative x-ray microanalysis of mast cell granules of fresh frozen dried ultrathin sections of the tongue of Wistar rats and tree frogs disclosed high concentrations of sulfur in rat mast cell granules and those of sulfur and magnesium in the tree frog granules. Their concentrations in tree frog mast cell granules were closely correlated (r=0.94).Fresh frozen dried ultrathin sections and fresh air-dried prints of the tree frog tongue and spleen and young red-eared turtle (ca. 6 g) spleen and heart blood were examined by a quantitative energy-dispersive x-ray microanalysis (X-650, Kevex-7000) for the element constituents of the granules of mast cells and basophils. The specimens were observed by transmission electron microscopy (TEM) (80-200 kV) and followed by scanning transmission electron microscopy (STEM) under an analytical electron microscope (X-650) at an acceleration voltage of 40 kV and a specimen current of 0.2 nA. A spot analysis was performed in a STEM mode for 100 s at a specimen current of 2 nA on the mast cell and basophil granules and other areas of the cells. Histamine was examined by the o-phthalaldehyde method.

Postero-Lateral Glands of Temnocephala Minor Haswell, 1888 (Platyhelminthes: Temnocephalida)

1996 ◽  
Vol 44 (1) ◽  
pp. 69
Author(s):  
LRG Cannon ◽  
NA Warson
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Lateral Margin ◽  
Freshwater Crayfish ◽  
Cherax Destructor ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Whole Mounts

Temnocephala minor Haswell, 1888 lives ectosymbiotically on the surface of the freshwater crayfish Cherax destructor in the Murray-Darling drainages of Australia. Some glands open on the postero-lateral margin and, being moderately refractory to many stains, can be overlooked in whole mounts and sections, and were, in fact, missed by Haswell. Observations were made on living worms with intra vitam dyes, and on whole mounts, wax sections and ultrathin sections using transmission electron microscopy (TEM) to characterise the secretion from these glands and ascertain its mode of manufacture. The function of the glands remains unknown although it appears non-adhesive.

The Technology in Sample Preparation for Transmission Electron Microscopy and Ultrastructure Observation of Lung Tissue in Rats with Experimental Silicosis

2014 ◽  
Vol 875-877 ◽  
pp. 695-698
Author(s):  
Qian Li ◽  
Li Juan Zhang ◽  
Fang Yang ◽  
Wen Li Zhang ◽  
Hong Xu
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Sample Preparation ◽  
Success Rate ◽  
Cutting Speed ◽  
Ultrastructural Changes ◽  
Histological Changes ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Perfusion Fixation

ts hard to get ideal ultrathin sections because of the adamant SiO2 dust in silicosis, after perfusion fixation methods and strict control of the cutting speed, improving the success rate of the Silicosis tissue TEM sample preparation of ultrathin sections,so we can more clearly and accurately observed ultrastructural changes of silicosis,and it also can offer morphological basis for research the silicosis organizations function histological changes.

Sperm morphology of Batrachyla (Anura: Leptodactylidae)

1989 ◽  
Vol 10 (2) ◽  
pp. 141-149 ◽  
Author(s):  
Boris Jorquera ◽  
Orlando Garrido ◽  
Emilio Pugin
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Phylogenetic Relationships ◽  
Sperm Morphology ◽  
Mature Sperm ◽  
Transmission Electron ◽  
Ultrathin Sections

AbstractThe structure of immature and mature sperm of the three species of Batrachyla were compared by using smears for light microscopy and ultrathin sections for transmission electron microscopy. Minor differences in length and some ultrastructural details support the notion that B. antartandica and B, taeniata are more closely related to each other than to B. leptopus. Comparisons of the sperm of Batrachyla with those of other anurans suggest the sperm morphology may be correlated with broad phylogenetic relationships as well as mode of fertilization.

Ultrathin sectioning and staining artifacts in transmission electron microscopy specimen preparation

1991 ◽  
Vol 49 ◽  
pp. 340-341
Author(s):  
Karen L. Klomparens
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Specimen Preparation ◽  
Transmission Electron Microscopy Specimen ◽  
Excessive Heat ◽  
Number Of Factors ◽  
Transmission Electron ◽  
Scientific Principles ◽  
Ultrathin Sections ◽  
Attention To Detail

Ultrathin sectioning followed by staining of sections are the final two steps in the preparation procedure for transmission electron microscope (TEM) specimens. While each requires an understanding of the scientific principles involved, these steps, perhaps more than any others, also require meticulous attention to detail and, for ultrathin sectioning, considerable skill and practice. Even with a properly fixed and infiltrated specimen in a well-polymerized block, there are a number of factors which can contribute to artifacts in ultrathin sections. Most can be corrected or at least minimized assuming that the ultramicrotome environment is well-situated; i.e., free of vibration, excessive heat, humidity, and air currents.

Adsorption Staining Methd For Ultrathin Frozen Sections

1980 ◽  
Vol 38 ◽  
pp. 760-763
Author(s):  
K. T. Tokuyasu
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Negative Staining ◽  
Positive Staining ◽  
Frozen Sections ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Immunocytochemical Studies ◽  
Ultrathin Frozen Sections

The successful routine use of cryoultramicrotomy to examine ultrathin sections by transmission electron microscopy requires the application of suitable staining to delineate the ultrastructure. While negative staining is quite effective for certain purposes1, 2, positive staining is more appropriate for immunocytochemical studies because it does not obscure the immunolabels.

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