Identification of Milk and Cheese Intake Biomarkers in Healthy Adults Reveals High Interindividual Variability of Lewis System–Related Oligosaccharides

ABSTRACT Background The use of biomarkers of food intake (BFIs) in blood and urine has shown great promise for assessing dietary intake and complementing traditional dietary assessment tools whose use is prone to misreporting. Objective Untargeted LC-MS metabolomics was applied to identify candidate BFIs for assessing the intake of milk and cheese and to explore the metabolic response to the ingestion of these foods. Methods A randomized controlled crossover study was conducted in healthy adults [5 women, 6 men; age: 23.6 ± 5.0 y; BMI (kg/m2): 22.1 ± 1.7]. After a single isocaloric intake of milk (600 mL), cheese (100 g), or soy-based drink (600 mL), serum and urine samples were collected postprandially up to 6 h and after fasting after 24 h. Untargeted metabolomics was conducted using LC-MS. Discriminant metabolites were selected in serum by multivariate statistical analysis, and their mass distribution and postprandial kinetics were compared. Results Serum metabolites discriminant for cheese intake had a significantly lower mass distribution than metabolites characterizing milk intake (P = 4.1 × 10−4). Candidate BFIs for milk or cheese included saccharides, a hydroxy acid, amino acids, amino acid derivatives, and dipeptides. Two serum oligosaccharides, blood group H disaccharide (BGH) and Lewis A trisaccharide (LeA), specifically reflected milk intake but with high interindividual variability. The 2 oligosaccharides showed related but opposing trends: subjects showing an increase in either oligosaccharide did not show any increase in the other oligosaccharide. This result was confirmed in urine. Conclusions New candidate BFIs for milk or cheese could be identified in healthy adults, most of which were related to protein metabolism. The increase in serum of LeA and BGH after cow-milk intake in adults calls for further investigations considering the beneficial health effects on newborns of such oligosaccharides in maternal milk. The trial is registered at clinicaltrials.gov as NCT02705560.

Download Full-text

Cultural Relevance of a Fruit and Vegetable Food Frequency Questionnaire

Canadian Journal of Dietetic Practice and Research ◽

10.3148/66.4.2005.231 ◽

2005 ◽

Vol 66 (4) ◽

pp. 231-236 ◽

Cited By ~ 3

Author(s):

Judy Paisley ◽

Marlene Greenberg ◽

Jess Haines

Keyword(s):

Food Frequency Questionnaire ◽

Dietary Assessment ◽

Cultural Relevance ◽

Assessment Tools ◽

Comparative Approach ◽

Fruit And Vegetable ◽

Food Frequency ◽

Research And Practice ◽

Vegetable Food ◽

Nutrition Research

Purpose: Canada’s multicultural population poses challenges for culturally competent nutrition research and practice. In this qualitative study, the cultural relevance of a widely used semiquantitative fruit and vegetable food frequency questionnaire (FFQ) was examined among convenience samples of adults from Toronto’s Cantonese-, Mandarin-, Portuguese-, and Vietnamesespeaking communities. Methods: Eighty-nine participants were recruited through community-based organizations, programs, and advertisements to participate in semi-structured interviews moderated in their native language. Data from the interviews were translated into English and transcribed for analysis using the constant comparative approach. Results: Four main themes emerged from the analysis: the cultural relevance of the foods listed on the FFQ, words with multiple meanings, the need for culturally appropriate portionsize prompts, and the telephone survey as a Western concept. Conclusions: This research highlights the importance of investing resources to develop culturally relevant dietary assessment tools that ensure dietary assessment accuracy and, more important, reduce ethnocentric biases in food and nutrition research and practice. The transferability of findings must be established through further research.

Download Full-text

Saliva and serum testosterone following oral testosterone undecanoate administration in normal and hypogonadal men

Acta Endocrinologica ◽

10.1530/acta.0.1020456 ◽

1983 ◽

Vol 102 (3) ◽

pp. 456-462 ◽

Cited By ~ 79

Author(s):

Th. Schürmeyer ◽

E. J. Wickings ◽

C. W. Freischem ◽

E. Nieschlag

Keyword(s):

Interindividual Variability ◽

Serum Testosterone ◽

Serum Samples ◽

Additional Increase ◽

Testosterone Undecanoate ◽

Absorption Pattern ◽

High Interindividual Variability ◽

Normal Men ◽

Hydrolysis Of ◽

Testosterone Ester

Abstract. Since saliva testosterone reflects the testosterone fraction available to target tissues the therapeutic effectiveness of orally administered testosterone undecanoate was assessed by measuring testosterone in serum and saliva. Matched saliva and serum samples were obtained from 12 normal men and 8 hypogonadal men before and at hourly intervals after the oral administration of 120 mg testosterone undecanoate. The test was repeated in 3 men after they had taken 40 mg testosterone undecanoate twice daily for 4 to 5 weeks. Following testosterone undecanoate administration serum and saliva testosterone always showed parallel increases. However, the absorption curves showed a high interindividual variability in the time when maximum concentrations were reached, as well as in the maximum levels themselves. The increases in serum and saliva testosterone were similar in normal and hypogonadal men. In normal men basal levels were reached 4 h after the maximum had occurred, while in hypogonadal men testosterone levels were not different from basal levels 2 h after the maximum. The study shows that testosterone undecanoate is well absorbed from the gut and releases significantly elevated amounts of testosterone which is available to target tissues. As the absorption pattern was always parallel in both fluids, hydrolysis of the circulating testosterone ester by the tissue ifself seems to effect no additional increase of testosterone in the tissue.

Download Full-text

Gender and microbiota determine hepatic bile acid and metabolic response to a single fast-food meal in healthy adults

10.1055/s-0040-1722026 ◽

2021 ◽

Author(s):

A Figge ◽

S Sydor ◽

C Wenning ◽

P Manka ◽

S Assmuth ◽

...

Keyword(s):

Bile Acid ◽

Fast Food ◽

Metabolic Response ◽

Healthy Adults ◽

Hepatic Bile

Download Full-text

Comparison of a web-based food record tool and a food-frequency questionnaire and objective validation using the doubly labelled water technique in a Swedish middle-aged population

Journal of Nutritional Science ◽

10.1017/jns.2016.29 ◽

2016 ◽

Vol 5 ◽

Cited By ~ 25

Author(s):

Sanna Nybacka ◽

Heléne Bertéus Forslund ◽

Elisabet Wirfält ◽

Ingrid Larsson ◽

Ulrika Ericson ◽

...

Keyword(s):

Energy Intake ◽

Large Scale ◽

Dietary Assessment ◽

Assessment Tools ◽

Future Research ◽

Food Record ◽

Frequency Method ◽

Doubly Labelled Water ◽

Web Based ◽

Food Frequency

AbstractTwo web-based dietary assessment tools have been developed for use in large-scale studies: the Riksmaten method (4-d food record) and MiniMeal-Q (food-frequency method). The aim of the present study was to examine the ability of these methods to capture energy intake against objectively measured total energy expenditure (TEE) with the doubly labelled water technique (TEEDLW), and to compare reported energy and macronutrient intake. This study was conducted within the pilot study of the Swedish CArdioPulmonary bioImage Study (SCAPIS), which included 1111 randomly selected men and women aged 50–64 years from the Gothenburg general population. Of these, 200 were enrolled in the SCAPIS diet substudy. TEEDLW was measured in a subsample (n 40). Compared with TEEDLW, both methods underestimated energy intake: −2·5 (sd 2·9) MJ with the Riksmaten method; −2·3 (sd 3·6) MJ with MiniMeal-Q. Mean reporting accuracy was 80 and 82 %, respectively. The correlation between reported energy intake and TEEDLW was r 0·4 for the Riksmaten method (P < 0·05) and r 0·28 (non-significant) for MiniMeal-Q. Women reported similar average intake of energy and macronutrients in both methods whereas men reported higher intakes with the Riksmaten method. Energy-adjusted correlations ranged from 0·14 (polyunsaturated fat) to 0·77 (alcohol). Bland–Altman plots showed acceptable agreement for energy and energy-adjusted protein and carbohydrate intake, whereas the agreement for fat intake was poorer. According to energy intake data, both methods displayed similar precision on energy intake reporting. However, MiniMeal-Q was less successful in ranking individuals than the Riksmaten method. The development of methods to achieve limited under-reporting is a major challenge for future research.

Download Full-text

Measuring energy, macro and micronutrient intake in UK children and adolescents: a comparison of validated dietary assessment tools

BMC Nutrition ◽

10.1186/s40795-019-0312-9 ◽

2019 ◽

Vol 5 (1) ◽

Cited By ~ 1

Author(s):

Linda A. Bush ◽

Jayne Hutchinson ◽

Jozef Hooson ◽

Marisol Warthon-Medina ◽

Neil Hancock ◽

...

Keyword(s):

Systematic Review ◽

Children And Adolescents ◽

Validation Studies ◽

Dietary Assessment ◽

Assessment Tools ◽

Food Diary ◽

Doubly Labelled Water ◽

Validation Data ◽

Review Of Reviews ◽

The Uk

Abstract Background Measuring dietary intake in children and adolescents can be challenging due to misreporting, difficulties in establishing portion size and reliance on recording dietary data via proxy reporters. The aim of this review was to present results from a recent systematic review of reviews reporting and comparing validated dietary assessment tools used in younger populations in the UK. Methods Validation data for dietary assessment tools used in younger populations (≤18 years) were extracted and summarised using results from a systematic review of reviews of validated dietary assessment tools. Mean differences and Bland-Altman limits of agreement (LOA) between the test and reference tool were extracted or calculated and compared for energy, macronutrients and micronutrients. Results Seventeen studies which reported validation of 14 dietary assessment tools (DATs) were identified with relevant nutrition information. The most commonly validated nutrients were energy, carbohydrate, protein, fat, calcium, iron, folate and vitamin C. There were no validated DATs reporting assessment of zinc, iodine or selenium intake. The most frequently used reference method was the weighed food diary, followed by doubly labelled water and 24 h recall. Summary plots were created to facilitate comparison between tools. On average, the test tools reported higher mean intakes than the reference methods with some studies consistently reporting wide LOA. Out of the 14 DATs, absolute values for LOA and mean difference were obtained for 11 DATs for EI. From the 24 validation results assessing EI, 16 (67%) reported higher mean intakes than the reference. Of the seven (29%) validation studies using doubly labelled water (DLW) as the reference, results for the test DATs were not substantially better or worse than those using other reference measures. Further information on the studies from this review is available on the www.nutritools.org website. Conclusions Validated dietary assessment tools for use with children and adolescents in the UK have been identified and compared. Whilst tools are generally validated for macronutrient intakes, micronutrients are poorly evaluated. Validation studies that include estimates of zinc, selenium, dietary fibre, sugars and sodium are needed.

Download Full-text

High interindividual variability in urinary fosfomycin concentrations in healthy female volunteers – authors' response

Clinical Microbiology and Infection ◽

10.1016/j.cmi.2018.10.017 ◽

2019 ◽

Vol 25 (2) ◽

pp. 256-257

Author(s):

R.A. Wijma ◽

B.C.P. Koch ◽

T. van Gelder ◽

J.W. Mouton

Keyword(s):

Interindividual Variability ◽

Healthy Female ◽

High Interindividual Variability

Download Full-text

Effect of Manual Data Cleaning on Nutrient Intakes using the Automated Self-Administered 24-Hour Dietary Assessment Tool (ASA24)

Current Developments in Nutrition ◽

10.1093/cdn/nzab005 ◽

2021 ◽

Author(s):

Yasmine Y Bouzid ◽

Joanne E Arsenault ◽

Ellen L Bonnel ◽

Eduardo Cervantes ◽

Annie Kan ◽

...

Keyword(s):

Energy Intake ◽

Assessment Tool ◽

Data Cleaning ◽

Dietary Assessment ◽

Assessment Tools ◽

Energy Requirements ◽

Nutrient Intakes ◽

Significant Difference ◽

Before And After ◽

Total Fat

Abstract Background Automated dietary assessment tools such as ASA24® are useful for collecting 24-hour recall data in large-scale studies. Modifications made during manual data cleaning may affect nutrient intakes. Objectives We evaluated the effects of modifications made during manual data cleaning on nutrients intakes of interest: energy, carbohydrate, total fat, protein, and fiber. Methods Differences in mean intake before and after data cleaning modifications for all recalls and average intakes per subject were analyzed by paired t-tests. Chi-squared test was used to determine whether unsupervised recalls had more open-ended text responses that required modification than supervised recalls. We characterized food types of text response modifications. Correlations between predictive energy requirements, measured total energy expenditure (TEE), and mean energy intake from raw and modified data were examined. Results After excluding 11 recalls with invalidating technical errors, 1499 valid recalls completed by 393 subjects were included in this analysis. We found significant differences before and after modifications for energy, carbohydrate, total fat, and protein intakes for all recalls (p < 0.05). Limiting to modified recalls, there were significant differences for all nutrients of interest, including fiber (p < 0.02). There was not a significantly greater proportion of text responses requiring modification for home compared to supervised recalls (p = 0.271). Predicted energy requirements correlated highly with TEE. There was no significant difference in correlation of mean energy intake with TEE for modified compared to raw data. Mean intake for individual subjects was significantly different for energy, protein, and fat intakes following cleaning modifications (p < 0.001). Conclusions Manual modifications can change mean nutrient intakes for an entire cohort and individuals. However, modifications did not significantly affect correlation of energy intake with predictive requirements and measured expenditure. Investigators can consider their research question and nutrients of interest when deciding to make cleaning modifications.

Download Full-text