scholarly journals S-RNase complexes and pollen rejection

2003 ◽  
Vol 54 (380) ◽  
pp. 123-130 ◽  
Author(s):  
F. Cruz-Garcia ◽  
C. N. Hancock ◽  
B. McClure
Keyword(s):  
Pollen Rejection

scholarly journals Two Loci Contribute Epistastically to Heterospecific Pollen Rejection, a Postmating Isolating Barrier Between Species

2017 ◽  
Vol 7 (7) ◽  
pp. 2151-2159 ◽  
Author(s):  
Jennafer A P Hamlin ◽  
Natasha A Sherman ◽  
Leonie C Moyle
Keyword(s):  
Female Choice ◽  
Genetic Basis ◽  
Reproductive Tract ◽  
Interspecific Cross ◽  
Solanum Species ◽  
Female Reproductive Tract ◽  
Solanum Pennellii ◽  
Heterospecific Pollen ◽  
Male Gametes ◽  
Pollen Rejection

Abstract Recognition and rejection of heterospecific male gametes occurs in a broad range of taxa, although the complexity of mechanisms underlying these components of postmating cryptic female choice is poorly understood. In plants, the arena for postmating interactions is the female reproductive tract (pistil), within which heterospecific pollen tube growth can be arrested via active molecular recognition and rejection. Unilateral incompatibility (UI) is one such postmating barrier in which pollen arrest occurs in only one direction of an interspecific cross. We investigated the genetic basis of pistil-side UI between Solanum species, with the specific goal of understanding the role and magnitude of epistasis between UI QTL. Using heterospecific introgression lines (ILs) between Solanum pennellii and S. lycopersicum, we assessed the individual and pairwise effects of three chromosomal regions (ui1.1, ui3.1, and ui12.1) previously associated with interspecific UI among Solanum species. Specifically, we generated double introgression (‘pyramided’) genotypes that combined ui12.1 with each of ui1.1 and ui3.1, and assessed the strength of UI pollen rejection in the pyramided lines, compared to single introgression genotypes. We found that none of the three QTL individually showed UI rejection phenotypes, but lines combining ui3.1 and ui12.1 showed significant pistil-side pollen rejection. Furthermore, double ILs (DILs) that combined different chromosomal regions overlapping ui3.1 differed significantly in their rate of UI, consistent with at least two genetic factors on chromosome three contributing quantitatively to interspecific pollen rejection. Together, our data indicate that loci on both chromosomes 3 and 12 are jointly required for the expression of UI between S. pennellii and S. lycopersicum, suggesting that coordinated molecular interactions among a relatively few loci underlie the expression of this postmating prezygotic barrier. In addition, in conjunction with previous data, at least one of these loci appears to also contribute to conspecific self-incompatibility (SI), consistent with a partially shared genetic basis between inter- and intraspecific mechanisms of postmating prezygotic female choice.

scholarly journals The ARC1 E3 Ligase Gene Is Frequently Deleted in Self-Compatible Brassicaceae Species and Has a Conserved Role in Arabidopsis lyrata Self-Pollen Rejection

2012 ◽  
Vol 24 (11) ◽  
pp. 4607-4620 ◽  
Author(s):  
Emily Indriolo ◽  
Pirashaanthy Tharmapalan ◽  
Stephen I. Wright ◽  
Daphne R. Goring
Keyword(s):  
E3 Ligase ◽  
Arabidopsis Lyrata ◽  
Pollen Rejection ◽  
Brassicaceae Species

scholarly journals A small asparagine-rich protein required for S-allele-specific pollen rejection in Nicotiana

1999 ◽  
Vol 96 (23) ◽  
pp. 13548-13553 ◽  
Author(s):  
B. McClure ◽  
B. Mou ◽  
S. Canevascini ◽  
R. Bernatzky
Keyword(s):  
Pollen Rejection ◽  
Allele Specific ◽  
S Allele

scholarly journals A Cysteine-Rich Protein, SpDIR1L, Implicated in S-RNase-Independent Pollen Rejection in the Tomato (Solanum Section Lycopersicon) Clade

2021 ◽  
Vol 22 (23) ◽  
pp. 13067
Author(s):  
Juan Vicente Muñoz-Sanz ◽  
Alejandro Tovar-Méndez ◽  
Lu Lu ◽  
Ru Dai ◽  
Bruce McClure
Keyword(s):  
Expression Profiles ◽  
Pollen Tubes ◽  
Upstream Region ◽  
Protein Accumulation ◽  
Reproductive Barriers ◽  
Solanum Pennellii ◽  
Unilateral Incompatibility ◽  
Pollen Rejection ◽  
Cysteine Rich Protein ◽  
Solanum Sect

Tomato clade species (Solanum sect. Lycopersicon) display multiple interspecific reproductive barriers (IRBs). Some IRBs conform to the SI x SC rule, which describes unilateral incompatibility (UI) where pollen from SC species is rejected on SI species’ pistils, but reciprocal pollinations are successful. However, SC x SC UI also exists, offering opportunities to identify factors that contribute to S-RNase-independent IRBs. For instance, SC Solanum pennellii LA0716 pistils only permit SC Solanum lycopersicum pollen tubes to penetrate to the top third of the pistil, while S. pennellii pollen penetrates to S. lycopersicum ovaries. We identified candidate S. pennellii LA0716 pistil barrier genes based on expression profiles and published results. CRISPR/Cas9 mutants were created in eight candidate genes, and mutants were assessed for changes in S. lycopersicum pollen tube growth. Mutants in a gene designated Defective in Induced Resistance 1-like (SpDIR1L), which encodes a small cysteine-rich protein, permitted S. lycopersicum pollen tubes to grow to the bottom third of the style. We show that SpDIR1L protein accumulation correlates with IRB strength and that species with weak or no IRBs toward S. lycopersicum pollen share a 150 bp deletion in the upstream region of SpDIR1L. These results suggest that SpDIR1L contributes to an S-RNase-independent IRB.

scholarly journals Intraspecific genetic variation underlying postmating reproductive barriers between species in the wild tomato clade (Solanum sect. Lycopersicon)

2019 ◽  
Author(s):  
Cathleen P Jewell ◽  
Simo Zhang ◽  
Matthew J. S. Gibson ◽  
Alejandro Tovar-Méndez ◽  
Bruce McClure ◽  
...  
Keyword(s):  
Intraspecific Variation ◽  
Interspecific Cross ◽  
Female Reproductive Tract ◽  
Reproductive Barriers ◽  
Solanum Pennellii ◽  
Wild Tomato ◽  
Genetic Components ◽  
Standing Variation ◽  
Pollen Rejection ◽  
In The Wild

AbstractA goal of speciation genetics is to understand how the genetic components underlying interspecific reproductive barriers originate within species. Unilateral incompatibility (UI) is a postmating prezygotic barrier in which pollen rejection in the female reproductive tract (style) occurs in only one direction of an interspecific cross. Natural variation in the strength of UI has been observed among populations within species in the wild tomato clade. In some cases, molecular loci underlying self-incompatibility (SI) are associated with this variation in UI, but the mechanistic connection between these intra- and inter-specific pollen rejection behaviors is poorly understood in most instances. We generated an F2 population between SI and SC genotypes of a single species, Solanum pennellii, to examine the genetic basis of intraspecific variation in the strength of UI against other species, and to determine whether loci underlying SI are genetically associated with this variation. We found that F2 individuals vary in the rate at which UI rejection occurs. One large effect QTL detected for this trait co-localized with the SI-determining S-locus. Moreover, individuals that expressed S-RNase—the S-locus protein involved in SI pollen rejection—in their styles had much more rapid UI responses compared to those without S-RNase protein. Our analysis shows that intraspecific variation at mate choice loci—in this case at loci that prevent self-fertilization—can contribute to variation in the strength of interspecific isolation, including postmating prezygotic barriers. Understanding the nature of such standing variation can provide insight into the accumulation of these barriers between diverging lineages.

scholarly journals Mechanism of self/nonself-discrimination in Brassica self-incompatibility

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Kohji Murase ◽  
Yoshitaka Moriwaki ◽  
Tomoyuki Mori ◽  
Xiao Liu ◽  
Chiho Masaka ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Specific Interaction ◽  
Md Simulations ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
Free Energies ◽  
Detailed Mechanism ◽  
Pollen Rejection ◽  
Cross Fertilization ◽  
Binding Free Energies

Abstract Self-incompatibility (SI) is a breeding system that promotes cross-fertilization. In Brassica, pollen rejection is induced by a haplotype-specific interaction between pistil determinant SRK (S receptor kinase) and pollen determinant SP11 (S-locus Protein 11, also named SCR) from the S-locus. Although the structure of the B. rapa S9-SRK ectodomain (eSRK) and S9-SP11 complex has been determined, it remains unclear how SRK discriminates self- and nonself-SP11. Here, we uncover the detailed mechanism of self/nonself-discrimination in Brassica SI by determining the S8-eSRK–S8-SP11 crystal structure and performing molecular dynamics (MD) simulations. Comprehensive binding analysis of eSRK and SP11 structures reveals that the binding free energies are most stable for cognate eSRK–SP11 combinations. Residue-based contribution analysis suggests that the modes of eSRK–SP11 interactions differ between intra- and inter-subgroup (a group of phylogenetically neighboring haplotypes) combinations. Our data establish a model of self/nonself-discrimination in Brassica SI.

scholarly journals The S –locus and unilateral incompatibility

2003 ◽  
Vol 358 (1434) ◽  
pp. 1133-1140 ◽  
Author(s):  
C. Nathan Hancock ◽  
Katsuhiko Kondo ◽  
Brian Beecher ◽  
Bruce McClure
Keyword(s):  
Control Mechanisms ◽  
S Locus ◽  
Pollen Rejection ◽  
Stigma Surface ◽  
Allele Specific ◽  
Genetic Mechanisms ◽  
Active Research ◽  
Compatible Pollen ◽  
Identification And Characterization

Plants have many ways to regulate the type of pollen that arrives on the stigma surface. Once there, further control mechanisms regulate compatibility. The latter controls are largely based on biochemical interactions that support compatible pollination and prevent incompatible matings. S–RNase–based self–incompatibility (SI) systems are the most phylogenetically widespread mechanisms for controlling pollination. Studies of Nicotiana establish a firm link between SI and unilateral interspecific incompatibility. Although implicated in both inter– and intraspecific compatibility, S–RNase operates through at least three distinct genetic mechanisms that differ in their dependence on non–S–RNase factors. Identification and characterization of these non–S–RNase factors is currently an area of active research. Searching for genetic and biochemical interactions with S–RNase can identify candidate non–S–RNase factors. HT–protein is one factor that is required for S –allele–specific pollen rejection in the Solanaceae. Major style arabinogalactan proteins such as TTS interact biochemically with S–RNase. These glycoproteins are known to interact with compatible pollen tubes and have long been suggested as possible recognition molecules. Their binding to S–RNase implies a link between stylar systems for compatibility and incompatibility. Thus, genetic and biochemical studies suggest a highly networked picture of pollen–pistil interactions.

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