scholarly journals Transcriptome sequencing and metabolite analysis reveals the role of delphinidin metabolism in flower colour in grape hyacinth

2014 ◽  
Vol 65 (12) ◽  
pp. 3157-3164 ◽  
Author(s):  
Qian Lou ◽  
Yali Liu ◽  
Yinyan Qi ◽  
Shuzhen Jiao ◽  
Feifei Tian ◽  
...  
Keyword(s):  
Transcriptome Sequencing ◽  
Flower Colour ◽  
Metabolite Analysis ◽  
Grape Hyacinth

scholarly journals Small RNA and transcriptome sequencing reveal the role of miR-199a-3p in inflammatory processes in cystic fibrosis airways

2018 ◽  
Vol 245 (4) ◽  
pp. 410-420 ◽  
Author(s):  
Pauline Bardin ◽  
Emmeline Marchal-Duval ◽  
Florence Sonneville ◽  
Sabine Blouquit-Laye ◽  
Nathalie Rousselet ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Small Rna ◽  
Transcriptome Sequencing ◽  
Inflammatory Processes

scholarly journals Cloning and Functional Characterization of Dihydroflavonol 4-Reductase Gene Involved in Anthocyanidin Biosynthesis of Grape Hyacinth

2019 ◽  
Vol 20 (19) ◽  
pp. 4743 ◽  
Author(s):  
Hongli Liu ◽  
Qian Lou ◽  
Junren Ma ◽  
Beibei Su ◽  
Zhuangzhuang Gao ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Flower Colour ◽  
Flower Color ◽  
Color Variation ◽  
Blue Flower ◽  
Blue Color ◽  
Color Development ◽  
High Preference ◽  
Grape Hyacinth

Grape hyacinth (Muscari spp.) is a popular ornamental plant with bulbous flowers noted for their rich blue color. Muscari species have been thought to accumulate delphinidin and cyanidin rather than pelargonidin-type anthocyanins because their dihydroflavonol 4-reductase (DFR) does not efficiently reduce dihydrokaempferol. In our study, we clone a novel DFR gene from blue flowers of Muscari. aucheri. Quantitative real-time PCR (qRT-PCR) and anthocyanin analysis showed that the expression pattern of MaDFR had strong correlations with the accumulation of delphinidin, relatively weak correlations with cyanidin, and no correations with pelargonidin. However, in vitro enzymatic analysis revealed that the MaDFR enzyme can reduce all the three types of dihydroflavonols (dihydrokaempferol, dihydroquercetin, and dihydromyricetin), although it most preferred dihydromyricetin as a substrate to produce leucodelphinidin, the precursor of blue-hued delphinidin. This indicated that there may be other functional genes responsible for the loss of red pelargonidin-based pigments in Muscari. To further verify the substrate-specific selection domains of MaDFR, an assay of amino acid substitutions was conducted. The activity of MaDFR was not affected whenever the N135 or E146 site was mutated. However, when both of them were mutated, the catalytic activity of MaDFR was lost completely. The results suggest that both the N135 and E146 sites are essential for the activity of MaDFR. Additionally, the heterologous expression of MaDFR in tobacco (Nicotiana tabacum) resulted in increasing anthocyanin accumulation, leading to a darker flower color, which suggested that MaDFR was involved in color development in flowers. In summary, MaDFR has a high preference for dihydromyricetin, and it could be a powerful candidate gene for genetic engineering for blue flower colour modification. Our results also make a valuable contribution to understanding the basis of color variation in the genus Muscari.

Gut Microbial Activity, Implications for Health and Disease: The Potential Role of Metabolite Analysis

2012 ◽  
Vol 11 (12) ◽  
pp. 5573-5585 ◽  
Author(s):  
Edna. P. Nyangale ◽  
Donald. S. Mottram ◽  
Glenn. R. Gibson
Keyword(s):  
Microbial Activity ◽  
Potential Role ◽  
Metabolite Analysis ◽  
Health And Disease

scholarly journals Visibility and attractiveness of Fritillaria (Liliaceae) flowers to potential pollinators

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Katarzyna Roguz ◽  
Laurence Hill ◽  
Sebastian Koethe ◽  
Klaus Lunau ◽  
Agata Roguz ◽  
...  
Keyword(s):  
Food Reward ◽  
Potential Role ◽  
Flower Colour ◽  
Floral Traits ◽  
Nectar Guides ◽  
Flower Colours ◽  
Visual Attraction ◽  
Floral Characters ◽  
Plant Pollinator

AbstractVisual floral characters play an important role in shaping plant-pollinator interactions. The genus Fritillaria L. (Liliaceae), comprising approximately 140 species, is described as displaying a remarkable variety of flower colours and sizes. Despite this variation in visual floral traits of fritillaries, little is known about the potential role of these features in shaping plant-pollinator interactions. Here, we seek to clarify the role of visual attraction in species offering a robust food reward for pollinators early in the spring, which is the case for Fritillaria. We also searched for potential tendencies in the evolution of floral traits crucial for plant-pollinator communication. The generality of species with green and purple flowers may indicate an influence of environmental factors other than pollinators. The flowers of the studied species seem to be visible but not very visually attractive to potential pollinators. The food rewards are hidden within the nodding perianth, and both traits are conserved among fritillaries. Additionally, visual floral traits are not good predictors of nectar properties. When in the flowers, pollinators are navigated by nectar guides in the form of contrasting nectary area colouration. Flower colour does not serve as a phenotypic filter against illegitimate pollinators—red and orange bird-pollinated fritillaries are visible to bees.

scholarly journals Genetics of flower colour in pink flowered “Rosea” and white flowers “Alba” in periwinkle Catharanthus roseus (L) G. Don

2021 ◽  
Vol 14 (3) ◽  
pp. 166-174
Author(s):  
Awad Hamza Abdelmageed ◽  
Mohamed Elkheir Abdelrahman ◽  
Hatil Hashim Alkamali
Keyword(s):  
Catharanthus Roseus ◽  
Flower Colour ◽  
Green Leaf ◽  
Single Pair ◽  
Leaf Lamina ◽  
Eye Color ◽  
Number Of Genes ◽  
Dark Green ◽  
Dark Green Leaf

Genetics of flower Colour in winka Catharanthus roseus (L) G. Don were in vestigate by inheritance two types (strains) of plants with different flowers colour were used in this study,pink corolla, and strong violet-purple eye color, and strong pink stem, and dark green leaf lamina (P), and White corolla, and yellow and greenish eye, and strong pink stem, and yellow and green leaf lamina (W) as parents, to determine the number of genes involved. This study was conducted at Horticulture Administration, Ministry of Agriculture, Kassala State, Sudan during for three years the period: Jan 2016 to Oct. 2020. First the two parents were covered to ensure self-pollination. Reciprocal cross has been carried out between the two inbred parents. The study showed that a single pair of genes is probably involved in flower colour and that gene for pink corolla, and strong violet-purple eye color, and strong pink stem, and dark green leaf lamina (P) is incompletely dominant over that for White corolla, and yellow and greenish eye, and strong pink stem, and yellow and green leaf lamina (W). The reciprocal crosses gave the same results indicating no role of cytoplasmic genes in the inheritance of these colors.

scholarly journals Transcriptome Sequencing and Metabolism Analysis Reveals the role of Cyanidin Metabolism in Dark-red Onion (Allium cepa L.) Bulbs

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Chunsha Zhang ◽  
Xiaojie Li ◽  
Zongxiang Zhan ◽  
Linjiao Cao ◽  
Aisong Zeng ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Transcriptome Sequencing ◽  
Allium Cepa L ◽  
Metabolism Analysis
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