scholarly journals Simple Detection of Hepatitis B Virus in Using Loop-Mediated Isothermal Amplification Method

2019 ◽  
Vol 184 (7-8) ◽  
pp. e275-e280 ◽  
Author(s):  
Hua-Wei Chen ◽  
Tatyana Belinskaya ◽  
Zhiwen Zhang ◽  
Wei-Mei Ching
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Eastern Mediterranean ◽  
Lamp Assay ◽  
Heat Treatment Condition ◽  
Isothermal Amplification ◽  
Blood Component ◽  
Easy Method ◽  
Loop Mediated Isothermal Amplification ◽  
B Virus

Abstract Introduction US Military and civilian personnel regularly deploy to regions that are endemic for the Hepatitis B virus (HBV), including the Western Pacific, Africa, Eastern Mediterranean, Southeast Asia, and Europe. When patients have life-threatening injuries that require any blood component that is not immediately available, they are typically transfused with locally collected fresh whole blood from a walking blood bank. Currently, there is no simple and easy method for sensitively screening fresh blood in deployed theaters of conflict. Materials and methods In order to fill the gap, we have developed a loop-mediated isothermal amplification (LAMP) assay to detect the presence of HBV in blood products. The primers were designed to target the gene of the pre-Surface/Surface antigen region of HBV. The amplification reaction mixture was incubated at 60°C for 60 min. The amplicon can be detected by a handheld fluorescence tube scanner or an immune-chromatography test strip. Results We were able to detect down to 10 copies of viral DNA by LAMP reaction for HBV DNA extracted from HBV-positive plasma. We also identified the optimal heat treatment condition (125°C for 10 min) for plasma specimens without requiring DNA extraction for the LAMP assay. The sensitivity of the assay was evaluated with polymerase chain reaction (PCR) confirmed HBV-positive samples. Using LAMP, we detected HBV in 107 out of 127 (84%) samples. Conclusion This LAMP assay has the potential to be used in resource-limited settings to improve the safety of locally collected blood in endemic regions.

scholarly journals Closed tube loop-mediated isothermal amplification assay for rapid detection of hepatitis B virus in human blood

Heliyon ◽  
2018 ◽  
Vol 4 (3) ◽  
pp. e00561 ◽  
Author(s):  
Nguyen Bao Quoc ◽  
Nguyen Doan Nguyen Phuong ◽  
Nguyen Ngoc Bao Chau ◽  
Do Thi Phuong Linh
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Human Blood ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
B Virus ◽  
Closed Tube ◽  
Amplification Assay

scholarly journals Development of a portable electrochemical loop mediated isothermal amplification (LAMP) device for detection of hepatitis B virus

RSC Advances ◽  
2018 ◽  
Vol 8 (61) ◽  
pp. 34954-34959 ◽  
Author(s):  
Nileththi Yasendra Jayanath ◽  
Loc Thai Nguyen ◽  
Thu Thi Vu ◽  
Lam Dai Tran
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
B Virus ◽  
Prototype Device ◽  
Set Up

A novel prototype device using LAMP and electrochemical drop cell set-up for rapid detection of hepatitis B virus.

scholarly journals Nanoparticle-Based Lateral Flow Biosensors Integrated With Loop-Mediated Isothermal Amplification for the Rapid and Visual Diagnosis of Hepatitis B Virus in Clinical Application

2021 ◽  
Vol 9 ◽  
Author(s):  
Xu Chen ◽  
Shoshi Wang ◽  
Yan Tan ◽  
Junfei Huang ◽  
Xingui Yang ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Cost Saving ◽  
Clinical Application ◽  
Limit Of Detection ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
B Virus

Hepatitis B virus (HBV) infection remains one of the major public health issues worldwide. Developing a rapid, sensitive, specific, easy-to-operate, and cost-saving approach for the diagnosis of HBV is essential for its therapy and prevention. Here, we first devised a novel approach, termed “loop-mediated isothermal amplification integrated with a nanoparticle-based lateral flow biosensor (LAMP-LFB),” for the detection of HBV in clinical application. The results indicated that a set of LAMP primers based on the S gene were valid for the establishment of HBV-LAMP-LFB. The optimal HBV-LAMP can be carried out at a constant temperature of 65°C for 40 min. The whole detection process, including HBV genomic DNA preparation (∼10 min), LAMP (40 min), and LFB reading (within 2 min), can be accomplished within 60 min. The limit of detection of the HBV-LAMP-LFB assay was 7.5 IU per test. The specificity of this assay was one hundred percent, and there was no cross-reactivity with other pathogens. Hence, these results indicated that the HBV-LAMP-LFB assay established in the current study is a sensitive, rapid, specific, visual, simple, and cost-saving method for the screening of HBV agents. More importantly, the HBV-LAMP-LFB has remarkable potential to develop a point-of-care testing in clinical application, especially in resource-scarce regions.

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