scholarly journals Evaluation of manual and image analysis quantification of DNA damage in the alkaline comet assay

Mutagenesis ◽  
1997 ◽  
Vol 12 (4) ◽  
pp. 209-214 ◽  
Author(s):  
P.J. McCarthy ◽  
S.F. Sweetman ◽  
P.G. McKenna ◽  
V.J. McKelvey-Martin
Keyword(s):  
Dna Damage ◽  
Image Analysis ◽  
Comet Assay ◽  
Alkaline Comet Assay

Dimeric NGF Mimetic Attenuates Hyperglycaemia and DNA Damage in Mice with Streptozotocin-Induced Early-Stage Diabetes

2020 ◽  
Vol 20 (3) ◽  
pp. 453-463
Author(s):  
Svetlana Yagubova ◽  
Aliy Zhanataev ◽  
Rita Ostrovskaya ◽  
Еlena Anisina ◽  
Тatiana Gudasheva ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Early Stage ◽  
Alkaline Comet Assay ◽  
Β Cells ◽  
Ghost Cells ◽  
Low Weight

Background: NGF deficiency is one of the reasons for reduced β-cells survival in diabetes. Our previous experiments revealed the ability of low-weight NGF mimetic, GK-2, to reduce hyperglycaemia in a model of advanced diabetes. The increase in DNA damage in advanced diabetes was repeatedly reported, while there were no data about DNA damage in the initial diabetes. Aim: The study aimed to establish whether DNA damage occurs in initial diabetes and whether GK-2 is able to overcome the damage. Methods: The early-stage diabetes was modelled in Balb/c mice by streptozotocin (STZ) (130 mg/kg, i.p.). GK-2 was administered at a dose of 0.5 mg/kg, i.p., subchronically. The evaluation of DNA damage was performed using the alkaline comet assay; the percentage of DNA in the tail (%TDNA) and the percentage of the atypical DNA comets (“ghost cells”) were determined. Results: STZ at this subthreshold dose produced a slight increase in glycemia and MDA. Meanwhile, pronounced DNA damage was observed, concerning mostly the percentage of “ghost cells” in the pancreas, the liver and kidneys. GK-2 attenuated the degree of hyperglycaemia and reduced the % of “ghost cells” and %TDNA in all the organs examined; this effect continued after discontinuation of the therapy. Conclusion: Early-stage diabetes is accompanied by DNA damage, manifested by the increase of “ghost cells” percentage. The severity of these changes significantly exceeds the degree of hyperglycaemia and MDA accumulation. GK-2 exerts an antihyperglycaemic effect and attenuates the degree of DNA damage. Our results indicate that the comet assay is a highly informative method for search of antidiabetic medicines.

scholarly journals Analysis of DNA damage in cells excreted in urine of cervical cancer patients using alkaline comet assay

2014 ◽  
Vol 7 (Suppl 1) ◽  
pp. P12
Author(s):  
Avani Patel ◽  
Mihir Shah ◽  
Pinaki Patel ◽  
Trupti Patel
Keyword(s):  
Cervical Cancer ◽  
Dna Damage ◽  
Comet Assay ◽  
Cancer Patients ◽  
Alkaline Comet Assay ◽  
In Cells

Monitoring of DNA damage in environmentally boron exposed people in Turkey by the alkaline comet assay

2007 ◽  
Vol 172 ◽  
pp. S72
Author(s):  
Ulku Undeger ◽  
Mehmet Korkmaz ◽  
Sevtap Aydin ◽  
Nursen Basaran
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Alkaline Comet Assay

Sperm DNA damage output parameters measured by the alkaline Comet assay and their importance

Andrologia ◽  
2016 ◽  
Vol 49 (2) ◽  
pp. e12608 ◽  
Author(s):  
L. Simon ◽  
K. I. Aston ◽  
B. R. Emery ◽  
J. Hotaling ◽  
D. T. Carrell
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Alkaline Comet Assay ◽  
Sperm Dna Damage ◽  
Sperm Dna

DNA damage and repair after exposure to sevoflurane in vivo, evaluated in Swiss albino mice by the alkaline comet assay and micronucleus test

2010 ◽  
Vol 51 (1) ◽  
pp. 79-86 ◽  
Author(s):  
G. Brozovic ◽  
N. Orsolic ◽  
R. Rozgaj ◽  
V. Kasuba ◽  
F. Knezevic ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Micronucleus Test ◽  
Alkaline Comet Assay ◽  
Dna Damage And Repair ◽  
Swiss Albino Mice ◽  
Albino Mice

DNA damage in lymphocytes of Indian rickshaw pullers as measured by the alkaline comet assay

2006 ◽  
Vol 47 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Alok Kumar Pandey ◽  
Mahima Bajpayee ◽  
Devendra Parmar ◽  
Subodh K. Rastogi ◽  
Neeraj Mathur ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Alkaline Comet Assay
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