scholarly journals Use of complementary DNA oligomers to probetrpleader transcript secondary structures involved in transcription pausing and termination

1984 ◽  
Vol 12 (7) ◽  
pp. 3295-3302 ◽  
Author(s):  
Robert Fisher ◽  
Charles Yanofsky
Keyword(s):  
Secondary Structures ◽  
Dna Oligomers ◽  
Complementary Dna

Base sequence- and T m-dependent DNA oligomer separation by open tubular capillary columns carrying complementary DNA oligomers as probes

2007 ◽  
Vol 388 (4) ◽  
pp. 919-928 ◽  
Author(s):  
Kamakshaiah Charyulu Devarayapalli ◽  
Seung Pil Pack ◽  
Nagendra Kumar Kamisetty ◽  
Mitsuru Nonogawa ◽  
Seiya Watanabe ◽  
...  
Keyword(s):  
Capillary Columns ◽  
Base Sequence ◽  
Dna Oligomers ◽  
Complementary Dna ◽  
Dna Oligomer Separation

Accelerated Chemical Reactions for Lab-on-a-Chip Applications Using Electrowetting-Induced Droplet Self-Oscillations

2006 ◽  
Vol 915 ◽  
Author(s):  
Joanna Aizenberg ◽  
Tom Krupenkin ◽  
Paul Kolodner
Keyword(s):  
Chemical Reactions ◽  
Light Emission ◽  
Lab On A Chip ◽  
Experimental Investigations ◽  
Dna Oligomers ◽  
Complementary Dna ◽  
Rapid Mixing ◽  
Biochemical Assays ◽  
Dna Strands ◽  
Viable Method

AbstractThis paper summarizes the results of experimental investigations of the feasibility of applying electrowetting-induced droplet self-oscillations to induce rapid mixing of small quantities of liquids. The concept was tested using video microscopy to monitor the mixing of passive colored dyes, of spatially-separated reactants that change color upon reaction, and of fluorescent DNA oligomers whose light emission vanishes upon hybridization with appropriately-functionalized complementary DNA strands. Droplet self-oscillation was found to increase the rate of mixing by factors ranging from 15 to 100 as compared with the rate of passive diffusion in undisturbed droplets. This demonstrates that self-oscillation-induced mixing is a viable method for substantially enhancing the speed of chemical reactions in general, and biochemical assays in particular, when performed in small volumes of liquids.

Role of the potential secondary structures in phage g4 origin of complementary dna strand synthesis

Gene ◽  
1988 ◽  
Vol 71 (2) ◽  
pp. 323-330 ◽  
Author(s):  
Sakai Hiroshi ◽  
Hiasa Hiroshi ◽  
Iwamoto Keiji ◽  
Horimoto Taizo ◽  
Komano Tohru ◽  
...  
Keyword(s):  
Secondary Structures ◽  
Complementary Dna ◽  
Dna Strand

Distinct functional contributions of three potential secondary structures in the phage G4 origin of complementary DNA strand synthesis

Gene ◽  
1989 ◽  
Vol 84 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Hiroshi Hiasa ◽  
Katsunori Tanaka ◽  
Hiroshi Sakai ◽  
Kanako Yoshida ◽  
Yoichi Honda ◽  
...  
Keyword(s):  
Secondary Structures ◽  
Complementary Dna ◽  
Dna Strand

Acyclic analogs of nucleosides based on tris(hydroxymethyl)phosphine oxide: synthesis and incorporation into short DNA oligomers

2015 ◽  
Vol 21 (5) ◽  
pp. 303-314 ◽  
Author(s):  
Barbara Nawrot ◽  
Olga Michalak ◽  
Barbara Mikołajczyk ◽  
Wojciech J. Stec
Keyword(s):  
Phosphine Oxide ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
Hydroxyl Groups ◽  
Dna Oligomers ◽  
Phase Synthesis ◽  
Complementary Dna ◽  
Dna Analogs ◽  
Dna Strands ◽  
Synthesis Protocol

AbstractTris-(hydroxymethyl)phosphine oxide (THPO) to a certain extent resembles a part of 2′-deoxyribofuranose, although it exists in an acyclic form only and the oxygen atom at the THPO phosphorus center provides additional hydration site or acceptor of hydrogen bonds. After proper protection of hydroxyl groups, THPO was functionalized with nucleobases and converted into phosphoramidite monomers suitable for incorporation into growing oligonucleotide chains within the solid phase synthesis protocol. The resultant THPO-DNA analogs show reduced affinity to complementary DNA strands, and are resistant towards snake venom and calf spleen exonucleases.

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