Differential expression of genes encoding the hypusine-containing translation initiation factor, elF-5A, in tobacco

Breast cancer affects women at relatively high frequency (1). We mined published microarray datasets (2, 3) to determine in an unbiased fashion and at the systems level genes most differentially expressed in the primary tumors of patients with breast cancer. We report here significant differential expression of the gene encoding eukaryotic translation initiation factor 4E-binding protein 2, EIF4EBP2, when comparing primary tumors of the breast to the tissue of origin, the normal breast. EIF4EBP2 was also differentially expressed in lymph node metastasis in human breast cancer. EIF4EBP2 mRNA was present at significantly lower quantities in tumors of the breast as compared to normal breast tissue. Analysis of human survival data revealed that expression of EIF4EBP2 in primary tumors of the breast was correlated with recurrence-free survival in patients with luminal B and HER2+ subtype cancer, demonstrating a relationship between primary tumor expression of a differentially expressed gene and patient survival outcomes influenced by PAM50 molecular subtype. EIF4EBP2 may be of relevance to initiation, maintenance or progression of cancers of the female breast.

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Differential expression of genes encoding tight junction proteins in colorectal cancer: frequent dysregulation of claudin-1, -8 and -12

International Journal of Colorectal Disease ◽

10.1007/s00384-006-0197-3 ◽

2006 ◽

Vol 22 (6) ◽

pp. 651-659 ◽

Cited By ~ 55

Author(s):

J. Gröne ◽

B. Weber ◽

E. Staub ◽

M. Heinze ◽

I. Klaman ◽

...

Keyword(s):

Colorectal Cancer ◽

Tight Junction ◽

Differential Expression ◽

Tight Junction Proteins ◽

Expression Of Genes ◽

Genes Encoding ◽

Differential Expression Of Genes ◽

Junction Proteins

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Crystal structure of the MIF4G domain of the Trypanosoma cruzi translation initiation factor EIF4G5

Acta Crystallographica Section F Structural Biology Communications ◽

10.1107/s2053230x19015061 ◽

2019 ◽

Vol 75 (12) ◽

pp. 738-743

Author(s):

Lucca Pietro Camillo dos Santos ◽

Bruno Moisés de Matos ◽

Brenda Cecilia de Maman Ribeiro ◽

Nilson Ivo Tonin Zanchin ◽

Beatriz Gomes Guimarães

Keyword(s):

Crystal Structure ◽

Translation Initiation ◽

Specific Interaction ◽

Three Dimensional ◽

Translation Initiation Factor ◽

Initiation Factor ◽

Dimensional Structure ◽

Initiation Factors ◽

Genes Encoding ◽

Rna Complexes

Kinetoplastida, a class of early-diverging eukaryotes that includes pathogenic Trypanosoma and Leishmania species, display key differences in their translation machinery compared with multicellular eukaryotes. One of these differences involves a larger number of genes encoding eIF4E and eIF4G homologs and the interaction pattern between the translation initiation factors. eIF4G is a scaffold protein which interacts with the mRNA cap-binding factor eIF4E, the poly(A)-binding protein, the RNA helicase eIF4A and the eIF3 complex. It contains the so-called middle domain of eIF4G (MIF4G), a multipurpose adaptor involved in different protein–protein and protein–RNA complexes. Here, the crystal structure of the MIF4G domain of T. cruzi EIF4G5 is described at 2.4 Å resolution, which is the first three-dimensional structure of a trypanosomatid MIF4G domain to be reported. Structural comparison with IF4G homologs from other eukaryotes and other MIF4G-containing proteins reveals differences that may account for the specific interaction mechanisms of MIF4G despite its highly conserved overall fold.

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