scholarly journals Marked Improvement of PAC and BAC Cloning Is Achieved Using Electroelution of Pulsed-Field Gel-Separated Partial Digests of Genomic DNA

1997 ◽  
Vol 25 (19) ◽  
pp. 3959-3961 ◽  
Author(s):  
S. J. Strong ◽  
Y. Ohta ◽  
G. W. Litman ◽  
C. T. Amemiya
Keyword(s):  
Genomic Dna ◽  
Marked Improvement ◽  
Pulsed Field ◽  
Bac Cloning

scholarly journals Molecular epidemiology of Salmonella enteritidis phage type 1b and 6a isolates in Portugal

2003 ◽  
Vol 131 (1) ◽  
pp. 607-612 ◽  
Author(s):  
A. R. SOARES ◽  
J. MACHADO
Keyword(s):  
Molecular Epidemiology ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Genomic Dna ◽  
Salmonella Enteritidis ◽  
Phage Type ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Main Pattern

Salmonella enterica serotype Enteritidis is an important serovar comprising 76% of salmonella isolates in Portugal in 2001.For better understand the epidemiology of salmonellosis, a total of 47 isolates of S. Enteritidis phage type (PT) 1b and 6a were analysed by pulsed-field gel electrophoresis (PFGE) and genomic DNA was subjected to macro restriction with XbaI. For PT1b isolates, only three different patterns were observed, and PT6a showed a total of 10 digestion patterns. Curiously, the main pattern among PT1b isolates seams quite similar to main pattern of PT6a isolates, but when the two patterns were analysed with Bionumerics, we observed that they exhibited some differences. It was concluded that, in 2001, there was one predominant pattern for PT1b and PT6a and, possibly, we were in presence of clonal strains that exists all over the country.

scholarly journals Direct observation of the gene organization of the complement C4 and 21-hydroxylase loci by pulsed field gel electrophoresis.

1989 ◽  
Vol 169 (5) ◽  
pp. 1803-1818 ◽  
Author(s):  
I Dunham ◽  
C A Sargent ◽  
R L Dawkins ◽  
R D Campbell
Keyword(s):  
Direct Observation ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Family Studies ◽  
Gene Organization ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Probes ◽  
Complement C4 ◽  
Pulsed Field ◽  
Definition Of

Pulsed field gel electrophoresis and enzymes that cut genomic DNA infrequently have been used to define large RFLPs at the human C4 loci. With the enzymes BssH II or Sac II, and C4 or 21-hydroxylase DNA probes, it has been possible to observe directly the number of C4 genes present on a haplotype, and also whether the C4 genes are long (6-7-kb intron present) or short (6-7-kb intron absent). Haplotypes that have either two long C4 genes or one long and one short C4 gene generate BssH II fragments of approximately 115 or approximately 105 kb, respectively. Haplotypes that have either a single long or a single short C4 gene generate BssH II fragments of approximately 80 or approximately 70 kb, respectively. This technique has been used to analyze the DNA isolated from PBMC and allows the complete definition of the C4 gene organization of an individual without the need for family studies.

scholarly journals DNA restriction patterns produced by pulsed-field gel electrophoresis in Moraxella catarrhalis isolated from different geographical areas

1999 ◽  
Vol 122 (3) ◽  
pp. 417-422 ◽  
Author(s):  
G. MARTINEZ ◽  
K. AHMED ◽  
C. H. ZHENG ◽  
K. WATANABE ◽  
K. OISHI ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Moraxella Catarrhalis ◽  
Genomic Dna ◽  
Respiratory Infections ◽  
Pulsed Field Gel Electrophoresis ◽  
Dynamic Changes ◽  
Restriction Patterns ◽  
Pulsed Field ◽  
Time Period ◽  
Dna Restriction

Pulsed field gel electrophoresis (PFGE) of the genomic DNA of Moraxella catarrhalis was done in 172 strains isolated from sputum of patients with respiratory infections in Nagasaki (130 strains), Europe (14 strains), Thailand (6 strains), Uganda (3 strains), Bangladesh (5 strains) and Kuwait (14 strains). Restriction endonuclease with SmaI generated 4–16 DNA fragments ranging from 1000 kb to 24·25 kb and was classified into 31 major groups. It was found that there were wide variations of DNA restriction patterns of strains isolated from the same and different geographical areas. DNA restriction patterns of strains isolated in Nagasaki during the last 12 years showed dynamic changes of the predominant strains in each time period. We conclude from this study that PFGE is a suitable method to document interstrain variation in M. catarrhalis.

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