STEM-23. LncRNA HOTAIR SPONGES miR301a-3p TO PROMOTE GLIOMA’S PROLIFERATION AND INVASION THROUGH THE ACTIVATION OF FosL1

2021 ◽  
Vol 23 (Supplement_6) ◽  
pp. vi26-vi26
Author(s):  
Pendelton King ◽  
Alyssa Guo ◽  
Jingwei Wan ◽  
Mingli Liu
Keyword(s):  
Human Cancer ◽  
Glioma Cells ◽  
Specific Expression ◽  
Data Set ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Lncrna Hotair ◽  
Proliferation And Invasion ◽  
A172 Glioma Cells

Abstract BACKGROUND Non-coding RNAs, including LncRNAs, function in regulating glioblastomas’ numerous oncogenic phenotypes, such as invasiveness and stemness of glioblastoma stem cells. These LncRNAs contain long non-encoding RNA transcripts >200 nucleotides in length, many of which show cell type-specific expression. In this project, we investigated how TRPM7, a promoter for glioma’s proliferation and invasion, regulates LncRNA and contributes to glioma tumorigenesis. METHODS 1) Total RNA, from either with A172 glioma cells or A172 glioma cells with TRPM7 knocked out (A172 KO), were extracted and then parallelly subjected to Human Cancer Pathway Finder, RT2 LncRNA PCR Array. 2) We then analyzed the prognostic role of LncRNAs using a publicly available bioinformatics data set (www.Oncolanc.org). 3) We examined the effects of TRPM7-regulated LncRNA, and its downstream molecules miR301a-3p and FosL1 oncogene, on the proliferation and invasion of glioma cells in A172 and PDX-L12 cells by either inhibition or activation of the above molecules. RESULTS 1) Data analysis from RT2 data resulted in a list of 10 downregulated and 7 upregulated LncRNAs whose transcripts are statistically significant with fold changes greater than 2.0 by TRPM7 knockout. 2) The results showed that TRPM7 functions as a positive regulator of LncRNA, HOTAIR, which is an unfavorable prognostic factor of 7-year overall survival in glioma patients. 3) In addition, we found that LncRNA HOTAIR sponges miR-301a-3p to promote proliferation, invasion, and stemness of glioma through upregulating the oncogene FosL1. 4) Furthermore, we identified that FosL1 is a novel prognostic marker of glioma patients. CONCLUSION Our results demonstrated the role of TRPM7-regulated LncRNA HOTAIR as a miRNA sponge in glioma, which shed new light on LncRNA-directed therapeutics in glioma.

The role of CpG methylation in cell type-specific expression of the aquaporin-5 gene

2007 ◽  
Vol 353 (4) ◽  
pp. 1017-1022 ◽  
Author(s):  
Johji Nomura ◽  
Akinori Hisatsune ◽  
Takeshi Miyata ◽  
Yoichiro Isohama
Keyword(s):  
Cpg Methylation ◽  
Cell Type ◽  
Aquaporin 5 ◽  
Specific Expression ◽  
Cell Type Specific Expression ◽  
Cell Type Specific

scholarly journals Conditional ablation and conditional rescue models for Casq2 elucidate the role of development and of cell-type specific expression of Casq2 in the CPVT2 phenotype

2018 ◽  
Vol 27 (9) ◽  
pp. 1533-1544 ◽  
Author(s):  
Daniel J Flores ◽  
ThuyVy Duong ◽  
Luke O Brandenberger ◽  
Apratim Mitra ◽  
Aditya Shirali ◽  
...  
Keyword(s):  
Cell Type ◽  
Specific Expression ◽  
Cell Type Specific Expression ◽  
Cell Type Specific

scholarly journals Nostopeptolide plays a governing role during cellular differentiation of the symbiotic cyanobacteriumNostoc punctiforme

2015 ◽  
Vol 112 (6) ◽  
pp. 1862-1867 ◽  
Author(s):  
Anton Liaimer ◽  
Eric J. N. Helfrich ◽  
Katrin Hinrichs ◽  
Arthur Guljamow ◽  
Keishi Ishida ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cellular Differentiation ◽  
Polyketide Synthase ◽  
Maldi Imaging ◽  
Free Living ◽  
Specific Expression ◽  
Living State ◽  
Cell Type Specific Expression ◽  
Cell Type Specific

Nostoc punctiformeis a versatile cyanobacterium that can live either independently or in symbiosis with plants from distinct taxa. Chemical cues from plants andN. punctiformewere shown to stimulate or repress, respectively, the differentiation of infectious motile filaments known as hormogonia. We have used a polyketide synthase mutant that accumulates an elevated amount of hormogonia as a tool to understand the effect of secondary metabolites on cellular differentiation ofN. punctiforme. Applying MALDI imaging to illustrate the reprogramming of the secondary metabolome, nostopeptolides were identified as the predominant difference in thepks2−mutant secretome. Subsequent differentiation assays and visualization of cell-type-specific expression of nostopeptolides via a transcriptional reporter strain provided evidence for a multifaceted role of nostopeptolides, either as an autogenic hormogonium-repressing factor or as a chemoattractant, depending on its extracellular concentration. Although nostopeptolide is constitutively expressed in the free-living state, secreted levels dynamically change before, during, and after the hormogonium differentiation phase. The metabolite was found to be strictly down-regulated in symbiosis withGunnera manicataandBlasia pusilla, whereas other metabolites are up-regulated, as demonstrated via MALDI imaging, suggesting plants modulate the fine-balanced cross-talk network of secondary metabolites withinN. punctiforme.

scholarly journals Deletion of the Clock Gene Period2 (Per2) in Glial Cells Alters Mood-Related Behavior in Mice

2020 ◽  
Author(s):  
Tomaz Martini ◽  
Jürgen A. Ripperger ◽  
Jimmy Stalin ◽  
Andrej Kores ◽  
Michael Stumpe ◽  
...  
Keyword(s):  
Glial Cells ◽  
Clock Gene ◽  
Clock Genes ◽  
Specific Expression ◽  
Adeno Associated Virus ◽  
Resistant Phenotype ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Physiological Pathways

AbstractThe circadian clock regulates many biochemical and physiological pathways, and lack of clock genes, such as Period (Per) 2, do not only affect circadian activity rhythms, but can also modulate food-anticipatory and mood-related behaviors. However, it is not known how cell-type specific expression of Per2 contributes to these behaviors. In this study, we find that Per2 in glial cells is important for balancing mood-related behaviors. Genetic and adeno-associated virus-mediated deletion of Per2 in glial cells of mice leads to a depression-resistant phenotype, as manifested in reduced despair and anxiety. This is paralleled by an increase of the GABA transporter 3 (Gat3) mRNA and a reduction of glutamate levels in the nucleus accumbens (NAc). Exclusive deletion of Per2 in glia of the NAc reduced despair, but had no influence on anxiety. Our data provide strong evidence for an important role of glial Per2 in regulating mood-related behavior.

scholarly journals Targeting Human Long Noncoding Transcripts by Endoribonuclease-Prepared siRNAs

2015 ◽  
Vol 20 (8) ◽  
pp. 1018-1026 ◽  
Author(s):  
Mirko Theis ◽  
Maciej Paszkowski-Rogacz ◽  
Ina Weisswange ◽  
Debojyoti Chakraborty ◽  
Frank Buchholz
Keyword(s):  
Cell Lines ◽  
Human Cancer ◽  
Cost Effective ◽  
Specific Expression ◽  
Protein Coding ◽  
Human Cancer Cell Lines ◽  
Localization Analysis ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Novel Transcripts

Broad sequencing enterprises such as the FANTOM or ENCODE projects have substantially extended our knowledge of the human transcriptome. They have revealed that a large portion of genomic DNA is actively transcribed and have identified a plethora of novel transcripts. Many newly identified transcripts belong to the class of long noncoding RNAs (lncRNAs), which range from a few hundred bases to multiple kilobases in length and harbor no protein-coding potential. Although the biological activity of some lncRNAs is understood, the functions of most lncRNAs remain elusive. Tools that allow rapid and cost-effective access to functional data of lncRNAs are therefore essential. Here, we describe the construction and validation of an endoribonuclease-prepared siRNA (esiRNA) library designed to target 1779 individual human lncRNAs by RNA interference. We present a compendium of lncRNA expression data for 11 human cancer cell lines. Furthermore, we show that the resource is suitable for combined knockdown and localization analysis. We discuss challenges in sequence annotation of lncRNAs with respect to their often low and cell type–specific expression and specify esiRNAs that are suitable for targeting lncRNAs in commonly used human cell lines.

scholarly journals Deletion of the clock gene Period2 (Per2) in glial cells alters mood-related behavior in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tomaz Martini ◽  
Jürgen A. Ripperger ◽  
Jimmy Stalin ◽  
Andrej Kores ◽  
Michael Stumpe ◽  
...  
Keyword(s):  
Glial Cells ◽  
Clock Genes ◽  
Circadian Activity ◽  
Specific Expression ◽  
Adeno Associated Virus ◽  
Knock Out ◽  
Cell Type Specific Expression ◽  
Cell Type Specific ◽  
Physiological Pathways

AbstractThe circadian clock regulates many biochemical and physiological pathways, and lack of clock genes, such as Period (Per) 2, affects not only circadian activity rhythms, but can also modulate feeding and mood-related behaviors. However, it is not known how cell-type specific expression of Per2 contributes to these behaviors. In this study, we find that Per2 in glial cells is important for balancing mood-related behaviors, without affecting circadian activity parameters. Genetic and adeno-associated virus-mediated deletion of Per2 in glial cells of mice leads to reduced despair and anxiety. This is paralleled by an increase of the GABA transporter 2 (Gat2/Slc6a13) and Dopamine receptor D3 (Drd3) mRNA, and a reduction of glutamate levels in the nucleus accumbens (NAc). Interestingly, neuronal Per2 knock-out also reduces despair, but does not influence anxiety. The change in mood-related behavior is not a result of a defective molecular clock, as glial Bmal1 deletion has no effect on neither despair nor anxiety. Exclusive deletion of Per2 in glia of the NAc reduced despair, but had no influence on anxiety. Our data provide strong evidence for an important role of glial Per2 in regulating mood-related behavior.

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