Living Cells, Lifeless Molecules

2021 ◽  
pp. 11-21
Author(s):  
Franklin M. Harold

Cells are composed of molecules that are lifeless but special, because most of them occur in nature only in the context of life. They are essential to all the workings of life, and no one single class holds life’s secret: life is an emergent property of the collective of molecules, assembled into the elaborate structures called cells. Cells come in great profusion, but all are variations on just two patterns of organization: prokaryotes, small and relatively simple microbes, both Bacteria and Archaea; and eukaryotes (Eukarya), the larger and more complex cells that make up all animals, plants, and fungi. The molecules of life, for all their diversity, again fall mainly into just a handful of categories. The bulk of living matter consists of proteins, nucleic acids, carbohydrates and lipids. Biomolecules belong to chemistry, but their functions in the process of living place them in the realm of biology.

2009 ◽  
Vol 187 (5) ◽  
pp. 589-596 ◽  
Author(s):  
Karmella A. Haynes ◽  
Pamela A. Silver

Synthetic biology aims to engineer novel cellular functions by assembling well-characterized molecular parts (i.e., nucleic acids and proteins) into biological “devices” that exhibit predictable behavior. Recently, efforts in eukaryotic synthetic biology have sprung from foundational work in bacteria. Designing synthetic circuits to operate reliably in the context of differentiating and morphologically complex cells presents unique challenges and opportunities for progress in the field. This review surveys recent advances in eukaryotic synthetic biology and describes how synthetic systems can be linked to natural cellular processes in order to manipulate cell behavior and to foster new discoveries in cell biology research.


2019 ◽  
Vol 10 (24) ◽  
pp. 6113-6119 ◽  
Author(s):  
Xiaotong Shen ◽  
Ying Zhang ◽  
Jianghui Sun ◽  
Hua Lu ◽  
Jin Ouyang ◽  
...  

Biodegradable nanosyringes were prepared to achieve efficient loading, delivery and release of biomolecules (enzymes, nucleic acids, and even silencing RNA (siRNA)) into living cells for amplification-based dual-detection of cancer biomarkers and gene therapy for cancer.


RSC Advances ◽  
2017 ◽  
Vol 7 (42) ◽  
pp. 26006-26018 ◽  
Author(s):  
W. Andrzejewska ◽  
M. Wilkowska ◽  
M. Chrabąszczewska ◽  
M. Kozak

Dicationic (also known as gemini or dimeric) bis-alkylimidazolium surfactants belong to a group of non-viral transfection systems proposed for the successful introduction of different types of nucleic acids (i.e., siRNA, DNA oligomers, and plasmid DNA) into living cells.


2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Stefania Croci ◽  
Alex Manicardi ◽  
Sara Rubagotti ◽  
Martina Bonacini ◽  
Michele Iori ◽  
...  

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3483
Author(s):  
Peng Nie ◽  
Yanfen Bai ◽  
Hui Mei

DNA, the fundamental genetic polymer of all living organisms on Earth, can be chemically modified to embrace novel functions that do not exist in nature. The key chemical and structural parameters for genetic information storage, heredity, and evolution have been elucidated, and many xenobiotic nucleic acids (XNAs) with non-canonical structures are developed as alternative genetic materials in vitro. However, it is still particularly challenging to replace DNAs with XNAs in living cells. This review outlines some recent studies in which the storage and propagation of genetic information are achieved in vivo by expanding genetic systems with XNAs.


2014 ◽  
Vol 6 (3) ◽  
pp. 1454-1461 ◽  
Author(s):  
Evgeny K. Apartsin ◽  
Marina Yu. Buyanova ◽  
Darya S. Novopashina ◽  
Elena I. Ryabchikova ◽  
Anton V. Filatov ◽  
...  

2019 ◽  
Vol 131 (34) ◽  
pp. 11889-11893
Author(s):  
Ying Zhou ◽  
Gang Wang ◽  
Pengchong Wang ◽  
Zeyao Li ◽  
Tieqiang Yue ◽  
...  
Keyword(s):  

2019 ◽  
Vol 72-73 ◽  
pp. S44
Author(s):  
S. Croci ◽  
A. Manicardi ◽  
C. Salvarani ◽  
A. Versari ◽  
R. Corradini ◽  
...  

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