Turner's syndrome patients lack tight junctions between uterine epithelial cells

Freeze-fracture cytochemistry with digitonin has been used to examine the cholesterol content of the plasma membrane of uterine epithelial cells during the early stages of pregnancy in the rat. Lesions caused by digitonin complexing with cholesterol were seen on both lateral and apical portions of the membrane but tight junctions and desmosomes were lesion-free. Compared with day 1 of pregnancy, lesions on the apical plasma membrane were much more extensive and some were of different morphology on day 6 - the day of blastocyst implantation. We consider mechanisms of lesion formation and interpret the results to indicate a higher content and perhaps a different organization of cholesterol in the apical plasma membrane on day 6 of pregnancy. We also suggest how this increase may occur.

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Trophoblast Cell Membrane Interactions at Implantation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100068540 ◽

1970 ◽

Vol 28 ◽

pp. 310-311

Author(s):

A. C. Enders

Keyword(s):

Epithelial Cells ◽

Cell Membrane ◽

Membrane Fusion ◽

Trophoblast Cell ◽

Membrane Interactions ◽

Uterine Epithelial Cells ◽

Functional Complex ◽

Cytotrophoblast Cells ◽

Complex Relationships ◽

Syncytial Trophoblast

The alteration in membrane relationships seen at implantation include 1) interaction between cytotrophoblast cells to form syncytial trophoblast and addition to the syncytium by subsequent fusion of cytotrophoblast cells, 2) formation of a wide variety of functional complex relationships by trophoblast with uterine epithelial cells in the process of invasion of the endometrium, and 3) in the case of the rabbit, fusion of some uterine epithelial cells with the trophoblast.Formation of syncytium is apparently a membrane fusion phenomenon in which rapid confluence of cytoplasm often results in isolation of residual membrane within masses of syncytial trophoblast. Often the last areas of membrane to disappear are those including a desmosome where the cell membranes are apparently held apart from fusion.

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Scanning and Transmission Electron Microscopy of the Endometrium in Women with Ovarian Dysgenesis (Turner'S Syndrome)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090725 ◽

1976 ◽

Vol 34 ◽

pp. 148-149

Author(s):

A. González-Angulo ◽

S. Armendares-Sagrera ◽

I. Ruíz de Chávez ◽

H. Marquez-Monter ◽

R. Aznar

Keyword(s):

Surface Epithelium ◽

Secretory Cells ◽

Turner's Syndrome ◽

Ciliated Cells ◽

Turner’S Syndrome ◽

Exogenous Hormone ◽

Ovarian Dysgenesis ◽

Transmission Electron ◽

Normal Women ◽

Microscopy Examination

It is a well documented fact that endometrial hyperplasia and adenocarcinoma may develop in women with Turner's syndrome who had received unopposed estrogen treatment (1), as well as in normal women under contraceptive medication with the sequential regime (2). The purpose of the present study was to characterize the possible changes in surface and glandular epithelium in these women who were treated with a sequential regime for a period of between three and eight years. The aim was to find organelle modifications which may lead to the understanding of the biology of an endometrium under exogenous hormone stimulation. Light microscopy examination of endometrial biopsies of nine patients disclosed a proliferative pattern; in two of these, there was focal hyperplasia. With the scanning electron microscope the surface epithelium in all biopsies showed secretory cells with microvilli alternating with non secretory ciliated cells. Regardless of the day of the cycle all biopsies disclosed a large number of secretory cells rich in microvilli (fig.l) with long and slender projections some of which were branching (fig. 2).

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ON THE FREQUENCY OF MALFORMATIONS IN THE DIFFERENT FORMS OF TURNER'S SYNDROME

Acta Endocrinologica ◽

10.1530/acta.0.077s048 ◽

1974 ◽

Vol 77 (1_Suppl) ◽

pp. S48 ◽

Cited By ~ 5

Author(s):

F. Majewski ◽

J. R. Bierich ◽

M. Barz ◽

W. F. Haberlandt ◽

M. Stoeckenius

Keyword(s):

Turner's Syndrome ◽

Turner’S Syndrome

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EVALUATION OF THE HYPOTHALAMIC-PITUITARY-GONADAL AXIS BY LHRH IN CHILDREN AND ADOLESCENTS WITH KLINEFELTER'S AND TURNER'S SYNDROME

Acta Endocrinologica ◽

10.1530/acta.0.077s026 ◽

1974 ◽

Vol 77 (1_Suppl) ◽

pp. S26 ◽

Cited By ~ 1

Author(s):

R. Illig ◽

M. Tolksdorf ◽

G. Mürset ◽

A. Prader

Keyword(s):

Children And Adolescents ◽

Turner's Syndrome ◽

Turner’S Syndrome

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Growth and development in girls with Turner's syndrome during early therapy with low doses of estradiol

Acta Endocrinologica ◽

10.1530/acta.0.112s157 ◽

1986 ◽

Vol 113 (4_Suppl) ◽

pp. S157-S163 ◽

Cited By ~ 8

Author(s):

K.W. KASTRUP ◽

_ _

Keyword(s):

Growth Velocity ◽

Final Height ◽

Bone Age ◽

Growth Spurt ◽

First Year ◽

Turner's Syndrome ◽

Turner’S Syndrome ◽

Early Therapy ◽

Group I ◽

Group Ii

Abstract Early therapy with a low dose of estrogen (estradiol-17β) was given to 33 girls with Turner's syndrome (T.s.) for a period of 4 years. The dose (0.25-2 mg/day) was adjusted every 3 months to maintain plasma estradiol in the normal concentration range for bone age. Growth velocity was compared with that of untreated girls with T.s. All girls were above age 10 years. Bone age was below 10 years in 11 girls (group I) and above 10 years in 22 girls (group II). Growth velocity in the first year of treatment in group I 7.5 ± 1.3 cm (SD) with mean SD score (SDS) of +4.3 and in group II 4.9 ± 1.3 with mean SDS of +3.5. Growth velocity decreased in the following years to 1.6 ± 1.0 cm, SDS -1.44 in group I and 0.9 ± 0.6cm, SDS -2.34 in group II during the fourth year. Withdrawal bleeding occurred in 16 girls of group II after the mean of 23 (range 15-33) months and in 3 girls of group I after 15 to 51 months of treatment. The treatment did not cause an inappropriate acceleration of pubertal development. Breast development appeared in most girls by 3 months of treatment. Pubic hair appeared by 12 months of treatment in group I; it was present in most girls in group II at start of treatment. Final height is known for 12 girls of group II; it was 144.2 ± 4.5 cm. The final height as predicted at the start of therapy was 142.2 ± 5.3 cm. Bone age advanced in the first year of treatment by 2 years. Early treatment with small doses of estrogens induces a growth spurt and normalizes the events of puberty. This will presumably decrease the psychological risks associated with abnormally delayed development.

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A highly sensitive sandwich enzyme immunoassay of urinary growth hormone in children with short stature, Turner's syndrome, and simple obesity

Acta Endocrinologica ◽

10.1530/acta.0.1210513 ◽

1989 ◽

Vol 121 (4) ◽

pp. 513-519 ◽

Cited By ~ 6

Author(s):

Hiroshi Tomita ◽

Masamichi Ogawa ◽

Takashi Kamijo ◽

Osamu Mori ◽

Eiji Ishikawa ◽

...

Keyword(s):

Short Stature ◽

Enzyme Immunoassay ◽

Gh Deficiency ◽

Urine Samples ◽

Turner's Syndrome ◽

Turner’S Syndrome ◽

Highly Sensitive ◽

Significant Difference ◽

Simple Obesity ◽

Sandwich Enzyme Immunoassay

Abstract. GH values were determined by a highly sensitive sandwich enzyme immunoassay in the 1st morning and/or 24-h accumulated urine samples in 94 children (short stature 70, including 14 with complete GH deficiency, 9 with partial GH deficiency, and 47 with GH-normal short stature; Turner's syndrome, 10, and simple obesity, 14). GH values were also determined in the 2nd to 4th urine samples taken on the same day together with the 1st morning urine in 5 of them. GH values in the 1st morning urine correlated significantly with those of the 24-h urine and with serum peak and mean GH values during nocturnal sleep as a physiological GH secretion test. The 2nd to 4th urines had lower GH concentrations than the 1st morning urine. The GH value of the 1st morning urine in complete GH deficiency was significantly lower than those in GH-normal short stature, partial GH deficiency and Turner's syndrome. However, no significant difference was detected in urinary GH values between complete GH deficiency and simple obesity. We conclude that 1st morning urinary GH estimation may be useful for differentiation of complete GH deficiency from other causes of short stature, but may be difficult for the distinction between complete GH deficiency and obesity with normal GH secretory ability.

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