Purpose. Estimation of ecological adaptability of hybrid (F3-5) populations of alfalfa for fodder and seed productivity against the background of increased soil acidity in different years of grass stand by determining its components – regression coefficient and stability variance. Methods. Field (conducting phenological observations and accounting), laboratory (accounting for seed productivity), mathematical and statistical (objective evaluation of the obtained experimental data). Results. The research was conducted in 2013-2020 in the fields of the Institute of Feed Research and Agriculture of Podillya of NAAS. 37 hybrid populations of F3-5 created with participation of alfalfa seed samples (Sinyukha (UJ0700134, Ukraine); Regina (UJ0700031, Ukraine); Yaroslavna (UJ0700225, Ukraine); Vika (Denmark); Mega (UJ0700365, Sweden), Grilys (Sweden) and the variable Gidrune (UJ0700699, Lithuania). Assessment of the ecological adaptability of hybrid (F3-5) populations of alfalfa, created on the basis of collection samples (Medicago sativa L., M. varia L.) of different ecological and geographical origin against the background of the increased soil acidity. The obtained values of stability variance confirm the difficulty of selection of alfalfa to increase the level of seed productivity compared to fodder, which are often positively affected by opposite hydrothermal conditions. Conclusions. Based on the results of the assessment of the ecological adaptability of hybrid (F3- 5) populations of alfalfa in conditions of increased soil acidity, the following combinations with a relatively high response to improved growing conditions, characterized by a potentially high level of fodder (Mega / Regina, Vika / Regina, Zhidrune / Regina, Zhidrune / Sinyukha, Mega / Grilys, Yaroslavna / Vika, Yaroslavna / Zhidrune, Grilys / Zhidrune, Vika / Mega) and seed productivity (Regina / Mega, Regina / Zhidrune, Sinyukha / Mega, Sinyukha / Yaroslavna, Sinyukha / Zhidrune, Grilys Mega, Grilys / Regina, Mega / Yaroslavna, Zhidrune / Vika, Mega / Zhidrune) are identified.