AtNOT1 Is a Novel Regulator of Gene Expression during Pollen Development

2019 ◽  
Vol 61 (4) ◽  
pp. 712-721 ◽  
Author(s):  
Kazuki Motomura ◽  
Toshihiro Arae ◽  
Haruka Araki-Uramoto ◽  
Yuya Suzuki ◽  
Hidenori Takeuchi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pollen Development ◽  
Male Gametophyte ◽  
Regulation Of Gene Expression ◽  
Specific Protein ◽  
Negative Regulation ◽  
Limited Information ◽  
Phenotypic Analysis ◽  
Pollen Stage ◽  
Mutant Pollen

Abstract Development of pollen, the male gametophyte of flowering plants, is tightly controlled by dynamic changes in gene expression. Recent research to clarify the molecular aspects of pollen development has revealed the involvement of several transcription factors in the induction of gene expression. However, limited information is available about the factors involved in the negative regulation of gene expression to eliminate unnecessary transcripts during pollen development. In this study, we revealed that AtNOT1 is an essential protein for proper pollen development and germination capacity. AtNOT1 is a scaffold protein of the AtCCR4–NOT complex, which includes multiple components related to mRNA turnover control in Arabidopsis. Phenotypic analysis using atnot1 heterozygote mutant pollen showed that the mature mutant pollen failed to germinate and also revealed abnormal localization of nuclei and a specific protein at the tricellular pollen stage. Furthermore, transcriptome analysis of atnot1 heterozygote mutant pollen showed that the downregulation of a large number of transcripts, along with the upregulation of specific transcripts required for pollen tube germination by AtNOT1 during late microgametogenesis, is important for proper pollen development and germination. Overall, our findings provide new insights into the negative regulation of gene expression during pollen development, by showing the severely defective phonotype of atnot1 heterozygote mutant pollen.

Positive and negative regulation of gene expression by insulin and glucagon: The model of L-type pyruvate kinase gene

Biochimie ◽  
1991 ◽  
Vol 73 (1) ◽  
pp. 41-45 ◽  
Author(s):  
M. Raymondjean ◽  
S. Voulont ◽  
M. Cognet ◽  
J.F. Decaux ◽  
N. Puzenat ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pyruvate Kinase ◽  
Regulation Of Gene Expression ◽  
Negative Regulation ◽  
Kinase Gene

The 3′ flanking region of the human ABO histo-blood group gene is involved in negative regulation of gene expression

2011 ◽  
Vol 13 (1) ◽  
pp. 22-29 ◽  
Author(s):  
Rie Sano ◽  
Tamiko Nakajima ◽  
Keiko Takahashi ◽  
Rieko Kubo ◽  
Shin Yazawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Blood Group ◽  
Regulation Of Gene Expression ◽  
Negative Regulation ◽  
Flanking Region

Ultrastructural characterization of male sterile33 (ms33) mutant in Arabidopsis affected in pollen desiccation and maturation

2001 ◽  
Vol 79 (1) ◽  
pp. 118-129 ◽  
Author(s):  
Houman Fei ◽  
Vipen K Sawhney
Keyword(s):  
Pollen Development ◽  
Ultrastructural Level ◽  
Mitotic Division ◽  
Wild Type ◽  
Pollen Maturation ◽  
Ultrastructural Characterization ◽  
In The Wild ◽  
Pollen Stage ◽  
Mutant Pollen

The MS33 gene in Arabidopsis is required for stamen filament growth and for pollen maturation. The objective of this study was to characterize the effects of ms33 mutation on pollen development at the ultrastructural level. There were no differences between the wild type and ms33 mutant pollen development before the first mitotic division of microspores. At the bicellular pollen stage, the first signs of abnormalities were observed in the ms33 tapetum, which started to degenerate early and released osmiophilic material in the anther locule. In ms33 pollen, the endintine was thicker, and exintine thinner, than in the wild type, and the mutant pollen had large vacuoles. Later in development, the mutant pollen underwent second mitosis and produced two normal-looking sperm cells; however, the intine was precociously formed, and there were abnormalities in tryphine deposition on the pollen wall, in the size of vacuoles, and in the formation of lipid bodies in the vegetative cell cytoplasm. Based on these observations it is suggested that mutation in the MS33 gene interferes with intine formation and tryphine deposition, both of which negatively affect pollen desiccation resulting in large, highly vacuolate pollen that are nonviable.Key words: Arabidopsis, male sterility, mutant, pollen, tapetum, ultrastructure.

Negative regulation of gene expression by TGF-?

1992 ◽  
Vol 32 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Lynn M. Matrisian ◽  
Gail L. Ganser ◽  
Lawrence D. Kerr ◽  
Ron W. Pelton ◽  
Lauren D. Wood
Keyword(s):  
Gene Expression ◽  
Regulation Of Gene Expression ◽  
Negative Regulation

scholarly journals Comparison of gene expression patterns of Kappaphycus alvarezii (Rhodophyta, Solieriaceae) under different light wavelengths and CO2 enrichment

2017 ◽  
Author(s):  
Thien Vun Yee ◽  
Kenneth Francis Rodrigues ◽  
Clemente Michael Wong Vui Ling ◽  
Wilson Yong Thau Lym
Keyword(s):  
Gene Expression ◽  
Carbon Fixation ◽  
Kappaphycus Alvarezii ◽  
Expression Patterns ◽  
Regulation Of Gene Expression ◽  
Light Regulation ◽  
Molecular Evidence ◽  
Limited Information ◽  
Metabolic Pattern ◽  
Regulated Gene Expression

AbstractTranscriptomes associated with the process of photosynthesis and carbon fixation have offered insights into the mechanism of gene regulation in terrestrial plants, however limited information is available as far as macroalgae are concerned. Intertidal red alga, Kappaphycus alvarezii is exposed to different wavelengths of light in their lives as light quantity and quality changes at different depths in seawater. This investigation aims to study the underlying mechanisms associated with photosynthesis and carbon fixation under specific light qualities and CO2 enrichment. Light regulation of gene expression has not been previously described for red algae. By using next generation sequencing, transcriptome profiling of K. alvarezii generated 76,871 qualified transcripts with a mean length of 979bp and a N50 length of 1,707bp and 55.83% transcripts were annotated on the basis of function. Blue, green and red light all have demonstrated roles in modulating light responses, such as changes in gene expression. Here we analysed the effects of light regulation on four selected photosynthesis aspects (light-harvesting complex, phycobilisomes, photosystems and photoreceptors). We observed that light-regulated gene expression in this species is not a single light response and different light qualities are transduced to regulate the same metabolic pattern. The carbon fixation pathway was analysed and key genes encoding enzymes involved in the carbon fixation pathway such as ppc, pepc, prk, pgk, ppdk, provided that unequivocal molecular evidence that most of the C3 and C4 pathway genes were actively transcribed in K. alvarezii. In addition to this the CO2 induced transcriptome suggested the possibility of shifting carbon metabolism pathway after acclimation to increased level of CO2. Impact of CO2 enrichment on the cultures has provided new insight into the response to rising CO2.

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