scholarly journals Engineering a de novo designed coiled-coil heterodimerization domain for the rapid detection, purification and characterization of recombinantly expressed peptides and proteins

1997 ◽  
Vol 10 (3) ◽  
pp. 299-299 ◽  
Author(s):  
B. Tripet ◽  
L. Yu ◽  
D. L. Bautista ◽  
W. Y. Wong ◽  
R. T. Irvin ◽  
...  
Keyword(s):  
Rapid Detection ◽  
De Novo ◽  
Coiled Coil ◽  
Purification And Characterization

Advanced approaches for the characterization of a de novo designed antiparallel coiled coil peptide

2005 ◽  
Vol 3 (7) ◽  
pp. 1189 ◽  
Author(s):  
Kevin Pagel ◽  
Karsten Seeger ◽  
Bettina Seiwert ◽  
Alessandra VillaCurrent address: J. W. Goethe ◽  
Alan E. Mark ◽  
...  
Keyword(s):  
De Novo ◽  
Coiled Coil ◽  
Antiparallel Coiled Coil

De novo design, synthesis and characterization of membrane-active peptides

2001 ◽  
Vol 29 (4) ◽  
pp. 559-564 ◽  
Author(s):  
J. D. Lear ◽  
H. Gratkowski ◽  
W. F. DeGrado
Keyword(s):  
De Novo ◽  
Coiled Coil ◽  
Water Soluble ◽  
Free Energies ◽  
Design Synthesis ◽  
Active Peptides ◽  
Hydrophobic Peptides ◽  
Level Of Understanding ◽  
Simple Sequence

Our current level of understanding of membrane-protein folding is primitive, but it is beginning to advance. Previously [Choma, Gratkowski, Lear and DeGrado (2000) Nat. Struct. Biol. 7, 161–166], we described studies of the association in detergent micelles of short, simple-sequence hydrophobic peptides modified from the sequence of the water-soluble, homodimeric coiled-coil GCN4-P1 peptide using the principle that the interiors of membrane proteins are similar to those of water-soluble proteins. Here, we discuss more quantitative aspects of the association equilibrium and compare the free energies of association of a number of mutant peptides designed to explore specific features responsible for the association.

scholarly journals Detection of antibodies neutralizing historical and emerging SARS-CoV-2 strains using a thermodynamically coupled de novo biosensor system

2021 ◽  
Author(s):  
Jason Z Zhang ◽  
Hsien-Wei Yeh ◽  
Alexandra C Walls ◽  
Basile IM Wicky ◽  
Kaiti Sprouse ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Neutralizing Antibodies ◽  
De Novo ◽  
Wild Type ◽  
Serum Samples ◽  
Biosensor System ◽  
Coupling Approach ◽  
Thermodynamic Coupling ◽  
Traditional Competition

With global vaccination efforts against SARS-CoV-2 underway, there is a need for rapid quantification methods for neutralizing antibodies elicited by vaccination and characterization of their strain dependence. Here, we describe a designed protein biosensor that enables sensitive and rapid detection of neutralizing antibodies against wild type and variant SARS-CoV-2 in serum samples. More generally, our thermodynamic coupling approach can better distinguish sample to sample differences in analyte binding affinity and abundance than traditional competition based assays.

Sign in / Sign up

Export Citation Format

Share Document