scholarly journals Genetic and Phenotypic Correlations Between Antibody Responses to Escherichia coli, Infectious Bursa Disease Virus (IBDV), and Newcastle Disease Virus (NDV), in Broiler Lines Selected on Antibody Response to Escherichia coli

2002 ◽  
Vol 81 (3) ◽  
pp. 302-308 ◽  
Author(s):  
R. Yunis ◽  
A. Ben-David ◽  
E.D. Heller ◽  
A. Cahaner
Keyword(s):  
Escherichia Coli ◽  
Antibody Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Antibody Responses ◽  
Phenotypic Correlations ◽  
Genetic And Phenotypic Correlations

scholarly journals A Recombinant Newcastle Disease Virus (NDV) Expressing VP2 Protein of Infectious Bursal Disease Virus (IBDV) Protects against NDV and IBDV

2004 ◽  
Vol 78 (18) ◽  
pp. 10054-10063 ◽  
Author(s):  
Zhuhui Huang ◽  
Subbiah Elankumaran ◽  
Abdul S. Yunus ◽  
Siba K. Samal
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Virus ◽  
Infectious Bursal Disease Virus ◽  
Antibody Responses ◽  
Infectious Bursal Disease ◽  
Vp2 Protein ◽  
Bursal Disease ◽  
Recombinant Newcastle Disease Virus

ABSTRACT Infectious bursal disease virus (IBDV) causes a highly immunosuppressive disease in chickens. Currently available, live IBDV vaccines can lead to generation of variant viruses. We have developed an alternative vaccine that will not create variant IBDV. By using the reverse genetics approach, we devised a recombinant Newcastle disease virus (NDV) vector from a commonly used vaccine strain LaSota to express the host-protective immunogen VP2 of a variant IBDV strain GLS-5. The gene encoding the VP2 protein of the IBDV was inserted into the most 3′-proximal locus of a full-length NDV cDNA for high-level expression. We successfully recovered the recombinant virus, rLaSota/VP2. The rLaSota/VP2 was genetically stable, at least up to 12 serial passages in chicken embryos, and was shown to express the VP2 protein. The VP2 protein was not incorporated into the virions of recombinant virus. Recombinant rLaSota/VP2 replicated to a titer similar to that of parental NDV strain LaSota in chicken embryos and cell cultures. To assess protective efficacy of the rLaSota/VP2, 2-day-old specific-pathogen-free chickens were vaccinated with the recombinant virus and challenged with a highly virulent NDV strain Texas GB or IBDV variant strain GLS-5 at 3 weeks postvaccination. Vaccination with rLaSota/VP2 generated antibody responses against both NDV and IBDV and provided 90% protection against NDV and IBDV. Booster immunization induced higher levels of antibody responses against both NDV and IBDV and conferred complete protection against both viruses. These results indicate that the recombinant NDV can be used as a vaccine vector for other avian pathogens.

scholarly journals Evaluation of Rapid Detection Kit against Avian Influenza A Virus and H5 Subtype for Field Sample

2017 ◽  
Vol 21 (1) ◽  
pp. 48 ◽  
Author(s):  
Michael Haryadi Wibowo ◽  
Tri Untari ◽  
Sidna Artanto ◽  
Krisdiana Putri ◽  
Surya Amanu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Avian Influenza ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Sensitivity And Specificity ◽  
Newcastle Disease ◽  
Rapid Test ◽  
Specificity Test ◽  
Test Kit ◽  
Reaction Test

Avian influenza virus is poultry viral disease, which causes high economic losses. Various efforts have been made to control the disease. One effort is required screening fast and precise diagnostic test. This study was aimed to determine the potential of rapid test kit of AIV/H5 Anigen Rapid Test for the detection of AI virus types A and subtype H5 in the field. Some tests were carried out, e.g.: the potential test, cross-reaction test, sensitivity and specificity test. Potency test was done to evaluate potential of detection limits of the kit, by having the test of serial dilution of AI virus positive control. Cross-reaction test was done to detect antigens other than AI virus H5N1, e.g.:  IB virus of Massachuset strain, IBV strain 4-91, Newcastle Disease virus, and Escherichia coli. Sensitivity and specificity test were applied to the filed samples which clinically and laboratory were confirmed as H5N1 positive. To confirm the result of rapid test was then being done by reverse transcriptase polymerase chain reaction. Based on these results it can be concluded that, Anigen Kit AIV/H5 Ag Rapid Test can detect antigen-containing samples having AI virus HA titer up to 26of type A virus, and up to 25 for subtype H5 virus. Anigen Kit AIV/H5 Ag Rapid Test showed no cross-reactions with Infectious Bronchitis virus, Newcastle Disease virus, and Escherichia coli. Anigen A Rapid Test Kit AIV Ag showed a sensitivity of 50% and specificity of 100%, while Anigen Ag Rapid Test Kit AIV/H5 showed a sensitivity of 25% and specificity is 100%.

Sign in / Sign up

Export Citation Format

Share Document