scholarly journals Effects of prolonged oral administration of aflatoxin B1 and fumonisin B1 in broiler chickens

2005 ◽  
Vol 84 (12) ◽  
pp. 1835-1840 ◽  
Author(s):  
M. Del Bianchi ◽  
C.A.F. Oliveira ◽  
R. Albuquerque ◽  
J.L. Guerra ◽  
B. Correa
Keyword(s):  
Oral Administration ◽  
Aflatoxin B1 ◽  
Broiler Chickens ◽  
Fumonisin B1

scholarly journals Toxicokinetics of Hydrolyzed Fumonisin B1 after Single Oral or Intravenous Bolus to Broiler Chickens Fed a Control or a Fumonisins-Contaminated Diet

Toxins ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 413 ◽  
Author(s):  
Gunther Antonissen ◽  
Siegrid De Baere ◽  
Barbara Novak ◽  
Dian Schatzmayr ◽  
Danica den Hollander ◽  
...  
Keyword(s):  
Oral Administration ◽  
Broiler Chickens ◽  
Clinical Symptoms ◽  
Control Diet ◽  
Plasma Concentrations ◽  
Fumonisin B1 ◽  
Ultra Performance Liquid Chromatography ◽  
Limit Of Quantification ◽  
Liquid Chromatography Tandem Mass ◽  
Hydrolyzed Fumonisin

The toxicokinetics (TK) of hydrolyzed fumonisin B1 (HFB1) were evaluated in 16 broiler chickens after being fed either a control or a fumonisins-contaminated diet (10.8 mg fumonisin B1, 3.3 mg B2 and 1.5 mg B3/kg feed) for two weeks, followed by a single oral (PO) or intravenous (IV) dose of 1.25 mg/kg bodyweight (BW) of HFB1. Fumonisin B1 (FB1), its partially hydrolyzed metabolites pHFB1a and pHFB1b, and fully hydrolyzed metabolite HFB1, were determined in chicken plasma using a validated ultra-performance liquid chromatography–tandem mass spectrometry method. None of the broiler chicken showed clinical symptoms of fumonisins (FBs) or HFB1 toxicity during the trial, nor was an aberration in body weight observed between the animals fed the FBs-contaminated diet and those fed the control diet. HFB1 was shown to follow a two-compartmental pharmacokinetic model with first order elimination in broiler chickens after IV administration. Toxicokinetic parameters of HFB1 demonstrated a total body clearance of 16.39 L/kg·h and an intercompartmental flow of 8.34 L/kg·h. Low levels of FB1 and traces of pHFB1b were found in plasma of chickens fed the FBs-contaminated diet. Due to plasma concentrations being under the limit of quantification (LOQ) after oral administration of HFB1, no toxicokinetic modelling could be performed in broiler chickens after oral administration of HFB1. Moreover, no phase II metabolites, nor N-acyl-metabolites of HFB1 could be detected in this study.

Effects of oral administration of aflatoxin B1 and fumonisin B1 in rabbits (Oryctolagus cuniculus)

2007 ◽  
Vol 170 (3) ◽  
pp. 201-208 ◽  
Author(s):  
R.B. Orsi ◽  
C.A.F. Oliveira ◽  
P. Dilkin ◽  
J.G. Xavier ◽  
G.M. Direito ◽  
...  
Keyword(s):  
Oral Administration ◽  
Aflatoxin B1 ◽  
Oryctolagus Cuniculus ◽  
Fumonisin B1

In vitro studies of the effects of aflatoxin B1 and fumonisin B1 on trypsin-like and collagenase-like activity from the hepatopancreas of white shrimp (Litopenaeus vannamei)

Aquaculture ◽  
2005 ◽  
Vol 250 (1-2) ◽  
pp. 399-410 ◽  
Author(s):  
Armando Burgos-Hernández ◽  
Sergio I. Farias ◽  
Wilfrido Torres-Arreola ◽  
Josafat M. Ezquerra-Brauer
Keyword(s):  
Aflatoxin B1 ◽  
Litopenaeus Vannamei ◽  
Fumonisin B1 ◽  
In Vitro Studies ◽  
White Shrimp

A lateral flow assay for simultaneous detection of Deoxynivalenol, Fumonisin B1 and Aflatoxin B1

Toxicon ◽  
2018 ◽  
Vol 156 ◽  
pp. 23-27 ◽  
Author(s):  
Songcheng Yu ◽  
Leiliang He ◽  
Fei Yu ◽  
Lie Liu ◽  
Chenling Qu ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Lateral Flow ◽  
Lateral Flow Assay

scholarly journals Experimental determination of the pharmacokinetic properties of trimethoprim and sulfamethoxazole combination in the blood serum of broiler chickens

2021 ◽  
Author(s):  
K Putecova ◽  
K Nedbalcova ◽  
I Bartejsova ◽  
M Zouharova ◽  
K Matiaskova ◽  
...  
Keyword(s):  
Oral Administration ◽  
Broiler Chickens ◽  
Animal Health ◽  
Pharmacokinetic Parameters ◽  
Serum Samples ◽  
Commercial Preparation ◽  
Single Oral Administration ◽  
Thermo Fisher Scientific ◽  
Medicinal Substances

A rapid, simple and highly efficient analytical method for the targeted determination of trimethoprim and sulfamethoxazole in serum samples has been developed and used to measure the pharmacokinetic curve of these medicinal substances after administration to chicken broilers. The pharmacokinetics properties of trimethoprim and sulfamethoxazole were investigated in clinically healthy broiler chickens after the single oral administration of the commercial preparation Methoxasol (Eurovet Animal Health, B.V., The Netherlands) at a dose of 0.275 ml/kg b.w. After a single dose drug administration, the chickens were sacrificed by decapitation under general anaesthesia by Isoflurin 1 000 mg/g (Vetpharma AH, Spain) and the blood was collected at precisely defined intervals: 15, 30, 45, 60, 90, 120, 180, 360 and 720 min after the administration. The serum concentrations of amoxicillin were determined using Q Exactive tandem mass spectrometer (Thermo Fisher Scientific, USA) in conjunction with liquid chromatography. The detected pharmacokinetic parameters of trimethoprim after the oral administration were C<sub>max</sub> = 2.1 ± 1.0 µg/ml; T<sub>max</sub> = 1.5 h; t<sub>½</sub> = 0.88 h; k<sub>el</sub> = 0.009 3 ± 0.001 1 1/h; AUC<sub>t</sub> = 2.901 ± 1.4 µg.h/ml; AUC<sub>∞</sub> = 2.907 ± 1.5 µg.h/ml; V<sub>d</sub> = 2.632 l/kg; Cl = 2.7 l/h. The pharmacokinetic parameters of sulfamethoxazole after the oral administration were C<sub>max</sub> = 47.1 ± 15.3 µg/ml; T<sub>max</sub> = 1 h; t<sub>½</sub> = 1.92 h; k<sub>el</sub> = 0.004 6 ± 0.000 3 1/h; AUC<sub>t</sub> = 89.676 ± 26.9 µg.h/ml; AUC<sub>∞</sub> = 94.612 ± 28.4 µg.h/ml; V<sub>d</sub> = 0.584 l/kg; Cl = 0.21 l/h. To the best of our knowledge, this is the first pharmacokinetic study of the combination of sulfamethoxazole and trimethoprim in broiler chickens.

scholarly journals Antibody Microarray Immunoassay for Simultaneous Quantification of Multiple Mycotoxins in Corn Samples

Toxins ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 415 ◽  
Author(s):  
Xian Zhang ◽  
Zuohuan Wang ◽  
Yun Fang ◽  
Renjie Sun ◽  
Tong Cao ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Goodness Of Fit ◽  
Limit Of Detection ◽  
Simultaneous Detection ◽  
Fumonisin B1 ◽  
Quantitative Detection ◽  
Antibody Microarray ◽  
Test Kit ◽  
Microarray Immunoassay

We developed and tested a prototype of an antibody microarray immunoassay for simultaneous quantitative detection of four typical mycotoxins (aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1) in corn samples. The test kit consisted of a nitrocellulose membrane layered with immobilized monoclonal antibodies against mycotoxins. During the assay, the mycotoxin-protein conjugates were biotinylated. The signal detection was enhanced by a combination of the biotin-streptavidin system and enhanced chemiluminescence (ECL). This improved the sensitivity of the assay. Under the optimized conditions, four calibration curves with goodness of fit (R2 > 0.98) were plotted. The results showed that the detection limits for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 were 0.21, 0.19, 0.09, and 0.24 ng/mL, with detection ranges of 0.47–55.69, 0.48–127.11, 0.22–31.36, and 0.56–92.57 ng/mL, respectively. The limit of detection (LOD) of this antibody microarray for aflatoxin B1, ochratoxin A, zearalenone, and fumonisin B1 in corn was 5.25, 4.75, 2.25, and 6 μg/kg, respectively. The recovery rates from the spiked samples were between 79.2% and 113.4%, with coefficient of variation <10%. The results of the analysis of commercial samples for mycotoxins using this new assay and the liquid chromatography-tandem mass spectrometry (LC-MS/MS) were comparable and in good agreement. This assay could also be modified for the simultaneous detection of other multiple mycotoxins, as well as low-weight analytes, hazardous to human health.

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