scholarly journals Mitogen-activated Protein Kinase Kinase Kinase 1 Protects against Nickel-induced Acute Lung Injury

2008 ◽  
Vol 104 (2) ◽  
pp. 405-411 ◽  
Author(s):  
Maureen Mongan ◽  
Zongqing Tan ◽  
Liang Chen ◽  
Zhimin Peng ◽  
Maggie Dietsch ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Protein Kinase ◽  
Lung Injury ◽  
Mitogen Activated Protein Kinase ◽  
Mitogen Activated Protein ◽  
Protein Kinase Kinase

scholarly journals Mitogen-activated protein kinase phosphatase 2, MKP-2, regulates early inflammation in acute lung injury

2012 ◽  
Vol 303 (3) ◽  
pp. L251-L258 ◽  
Author(s):  
Timothy T. Cornell ◽  
Andrew Fleszar ◽  
Walker McHugh ◽  
Neal B. Blatt ◽  
Ann Marie Le Vine ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Protein Kinase ◽  
Lung Injury ◽  
Neutrophil Infiltration ◽  
Mitogen Activated Protein Kinase ◽  
Molecular Response ◽  
Proinflammatory Response ◽  
Mapk Pathways ◽  
Mitogen Activated Protein

Acute lung injury (ALI) is mediated by an early proinflammatory response resulting from either a direct or indirect insult to the lung mediating neutrophil infiltration and consequent disruption of the alveolar capillary membrane ultimately leading to refractory hypoxemia. The mitogen-activated protein kinase (MAPK) pathways are a key component of the molecular response activated by those insults triggering the proinflammatory response in ALI. The MAPK pathways are counterbalanced by a set of dual-specific phosphatases (DUSP) that deactivate the kinases by removing phosphate groups from tyrosine or threonine residues. We have previously shown that one DUSP, MKP-2, regulates the MAPK pathway in a model of sepsis-induced inflammation; however, the role of MKP-2 in modulating the inflammatory response in ALI has not been previously investigated. We utilized both MKP-2-null (MKP-2−/−) mice and MKP-2 knockdown in a murine macrophage cell line to elucidate the role of MKP-2 in regulating inflammation during ALI. Our data demonstrated attenuated proinflammatory cytokine production as well as decreased neutrophil infiltration in the lungs of MKP-2−/− mice following direct, intratracheal LPS. Importantly, when challenged with a viable pathogen, this decrease in neutrophil infiltration did not impact the ability of MKP-2−/− mice to clear either gram-positive or gram-negative bacteria. Furthermore, MKP-2 knockdown led to an attenuated proinflammatory response and was associated with an increase in phosphorylation of ERK and induction of a related DUSP, MKP-1. These data suggest that altering MKP-2 activity may have therapeutic potential to reduce lung inflammation in ALI without impacting pathogen clearance.

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