scholarly journals Determination of inter- and intra-species genetic relationships among six Eucalyptus species based on inter-simple sequence repeats (ISSR)

2005 ◽  
Vol 25 (10) ◽  
pp. 1295-1302 ◽  
Author(s):  
T. Balasaravanan ◽  
P. Chezhian ◽  
R. Kamalakannan ◽  
M. Ghosh ◽  
R. Yasodha ◽  
...  
Keyword(s):  
Simple Sequence Repeats ◽  
Genetic Relationships ◽  
Eucalyptus Species ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

Genetic relationships of sesame germplasm collection as revealed by inter-simple sequence repeats

2002 ◽  
Vol 121 (3) ◽  
pp. 259-262 ◽  
Author(s):  
D. H. KIM ◽  
G. ZUR ◽  
Y. DANIN-POLEG ◽  
S. W. LEE ◽  
K. B. SHIM ◽  
...  
Keyword(s):  
Simple Sequence Repeats ◽  
Genetic Relationships ◽  
Germplasm Collection ◽  
Inter Simple Sequence Repeats ◽  
Simple Sequence

scholarly journals Use of Inter-Simple Sequence Repeats and Amplified Fragment Length Polymorphisms to Analyze Genetic Relationships Among Small Grain-Infecting Species of Ustilago

2003 ◽  
Vol 93 (2) ◽  
pp. 167-175 ◽  
Author(s):  
J. G. Menzies ◽  
G. Bakkeren ◽  
F. Matheson ◽  
J. D. Procunier ◽  
S. Woods
Keyword(s):  
Fragment Length ◽  
Simple Sequence Repeats ◽  
Genetic Relationships ◽  
Molecular Techniques ◽  
Smut Fungi ◽  
Inter Simple Sequence Repeats ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Dna Profiles ◽  
Simple Sequence

In the smut fungi, few features are available for use as taxonomic criteria (spore size, shape, morphology, germination type, and host range). DNA-based molecular techniques are useful in expanding the traits considered in determining relationships among these fungi. We examined the phylogenetic relationships among seven species of Ustilago (U. avenae, U. bullata, U. hordei, U. kolleri, U. nigra, U. nuda, and U. tritici) using inter-simple sequence repeats (ISSRs) and amplified fragment length polymorphisms (AFLPs) to compare their DNA profiles. Fifty-four isolates of different Ustilago spp. were analyzed using ISSR primers, and 16 isolates of Ustilago were studied using AFLP primers. The variability among isolates within species was low for all species except U. bullata. The isolates of U. bullata, U. nuda, and U. tritici were well separated and our data supports their speciation. U. avenae and U. kolleri isolates did not separate from each other and there was little variability between these species. U. hordei and U. nigra isolates also showed little variability between species, but the isolates from each species grouped together. Our data suggest that U. avenae and U. kolleri are monophyletic and should be considered one species, as should U. hordei and U. nigra.

scholarly journals Lemons: Diversity and Relationships with Selected Citrus Genotypes as Measured with Nuclear Genome Markers

2001 ◽  
Vol 126 (3) ◽  
pp. 309-317 ◽  
Author(s):  
O. Gulsen ◽  
M.L. Roose
Keyword(s):  
Simple Sequence Repeats ◽  
Phylogenetic Trees ◽  
Nuclear Genome ◽  
Group Method ◽  
Arithmetic Average ◽  
Pair Group ◽  
Inter Simple Sequence Repeats ◽  
Ssr Loci ◽  
High Level ◽  
Simple Sequence

Inter-simple sequence repeats (ISSR), simple sequence repeats (SSR) and isozymes were used to measure genetic diversity and phylogenetic relationships among 95 Citrus L. accessions including 57 lemons [C. limon (L.) Burm. f.], related taxa, and three proposed ancestral species, C. maxima (Burm.) Merrill (pummelo), C. medica L. (citron), and C. reticulata Blanco (mandarin). The ancestry of lemons and several other suspected hybrids was also studied. Five isozyme and five SSR loci revealed relatively little variation among most lemons, but a high level of variation among the relatively distant Citrus taxa. Eight ISSR primers amplified a total of 103 polymorphic fragments among the 83 accessions. Similarity matrices were calculated and phylogenetic trees derived using unweighted pair-group method, arithmetic average cluster analysis. All lemons, rough lemons, and sweet lemons, as well as some other suspected hybrids, clustered with citrons. Most lemons (68%) had nearly identical marker phenotypes, suggesting they originated from a single clonal parent via a series of mutations. Citrons contributed the largest part of the lemon genome and a major part of the genomes of rough lemons, sweet lemons, and sweet limes. Bands that characterize C. reticulata and C. maxima were detected in lemons, suggesting that these taxa also contributed to the pedigree of lemon.

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