Genome size variation in Deschampsia cespitosa sensu lato (Poaceae) in Eurasia

The grass Deschampsia cespitosa is a variable taxon out of which many varieties, subspecies and endemic species have been separated. In this paper, the variation in genome size (GS) and ploidy of this grass including several of its subspecies and two related species in Eurasia was investigated by flow cytometric (FCM) measurements. GS and ploidy data were also related to specific environments and reproduction mode. Ploidy levels found by FCM were confirmed by chromosome counts of diploid (2n = 28) and tetraploid (2n = 52) samples. Seminiferous (seed bearing) D. cespitosa was mainly diploid (GS between 3.754 and 5.438 pg/1C). GS variation in diploids showed a geographic pattern with a significant difference (H = 41,441, P < 0.001) between European (median = 4.377 pg) and Asian (median = 4.881 pg) accessions. Genome size (1C) in tetraploids ranged from 7.9426 to 9.0399 pg. Tetraploid seminiferous D. cespitosa was found mostly in disturbed habitats in western and southern Europe, while tetraploids in Asia were registered in wet Arctic habitats. Genome size (1C between 8.3278 and 8.8603 pg) of the pseudoviviparous plants (spikelets produce plantlets asexually) of wet habitats in central and northern Europe indicated tetraploidy. A putative triploid (GS 6.6817 pg) was detected in Iceland. Summing up, we found a high variation in GS on the geographic scale with significant regional differences in diploid D. cespitosa. Among the tetraploids, the asexually reproducing plants were bound to specific habitats, while the seminiferous plants showed a habitat preference similar to the diploids.

The genus Deschampsia and the epithet “ alpina”

The epithet “alpina” has been recurrently used in the genus Deschampsia to name plants located in northern regions of Europe, Asia and North America, as a species (Deschampsia alpina (L.) Roem. &amp; Schult.), but also in infraspecific categories (Deschampsia cespitosa subsp. alpina Tzvel. and Deschampsia cespitosa var. alpina Schur.). The morphological and molecular available evidence suggests the existence of a single species, Deschampsia cespitosa (L.) P. Beauv., in which individuals belonging to the same morphological gradient have received different names in different taxonomic categories throughout its wide distribution range. An evaluation of the available names indicates that all uses of the epithet “alpina” are illegitimate. A new combination is proposed at the infraspecific level as Deschampsia cespitosa subsp. neoalpina Chiapella, Xue &amp; Greimler.

Vascular plants of Victoria Island (Northwest Territories and Nunavut, Canada): a specimen-based study of an Arctic flora

Victoria Island in Canada’s western Arctic is the eighth largest island in the world and the second largest in Canada. Here, we report the results of a floristic study of vascular plant diversity of Victoria Island. The study is based on a specimen-based dataset comprising 7031 unique collections from the island, including some 2870 new collections gathered between 2008 and 2019 by the authors and nearly 1000 specimens variously gathered by N. Polunin (in 1947), M. Oldenburg (1940s–1950s) and S. Edlund (1980s) that, until recently, were part of the unprocessed backlog of the National Herbarium of Canada and unavailable to researchers. Results are presented in an annotated checklist, including keys and distribution maps for all taxa, citation of specimens, comments on taxonomy, distribution and the history of documentation of taxa across the island, and photographs for a subset of taxa. The vascular plant flora of Victoria Island comprises 38 families, 108 genera, 272 species, and 17 additional taxa. Of the 289 taxa known on the island, 237 are recorded from the Northwest Territories portion of the island and 277 from the Nunavut part. Thirty-nine taxa are known on the island from a single collection, seven from two collections and three from three collections. Twenty-one taxa in eight families are newly recorded for the flora of Victoria Island: Artemisia tilesii, Senecio lugens, Taraxacum scopulorum (Asteraceae); Crucihimalaya bursifolia, Draba fladnizensis, D. juvenilis, D. pilosa, D. simmonsii (Brassicaceae); Carex bigelowii subsp. bigelowii, Eriophorum russeolum subsp. albidum (Cyperaceae); Anthoxanthum monticola subsp. monticola, Bromus pumpellianus, Deschampsia cespitosa subsp. cespitosa, D. sukatschewii, Festuca rubra subsp. rubra, Lolium perenne, Poa pratensis subsp. pratensis (Poaceae); Stuckenia filiformis (Potamogetonaceae); Potentilla × prostrata (Rosaceae); Galium aparine (Rubiaceae); and Salix ovalifolia var. ovalifolia (Salicaceae). Eight of these are new to the flora of the Canadian Arctic Archipelago: Senecio lugens, Draba juvenilis, D. pilosa, Anthoxanthum monticola subsp. monticola, Bromus pumpellianus, Deschampsia cespitosa subsp. cespitosa, Poa pratensis subsp. pratensis and Salix ovalifolia var. ovalifolia. One of these, Galium aparine, is newly recorded for the flora of Nunavut. Four first records for Victoria Island are introduced plants discovered in Cambridge Bay in 2017: three grasses (Festuca rubra subsp. rubra, Lolium perenne, and Poa pratensis subsp. pratensis) and Galium aparine. One taxon, Juncus arcticus subsp. arcticus, is newly recorded from the Northwest Territories. Of the general areas on Victoria Island that have been botanically explored the most, the greatest diversity of vascular plants is recorded in Ulukhaktok (194 taxa) and the next most diverse area is Cambridge Bay (183 taxa). The floristic data presented here represent a new baseline on which continued exploration of the vascular flora of Victoria Island – particularly the numerous areas of the island that remain unexplored or poorly explored botanically – will build.

Deschampsia cespitosa subsp. parviflora (Poaceae) - an overlooked woodland grass

Deschampsia cespitosa (L.) P. Beauv. subsp. parviflora (Thuill.) Dumort was, to British and Irish botanists, a little known taxon prior to1988, and current distribution mapping shows a marked geographical recording bias. It is confirmed as being primarily a woodland taxon, at low altitudes; modal mean altitude 50-75 m AOD, with 97% of locations at ≤300 m AOD. A combination of woodland or shaded habitat, bright green narrow leaves, and delicate panicle, with small spikelets, is suggestive of subsp. parviflora. In combination, degree of leaf blade scabridity on the adaxial surface, appearance of papillae on flat surfaces of adaxial ridges of the leaf, and spikelet length, provide the most reliable means of distinguishing this subspecies from subsp. cespitosa. There is no reason to suspect that subsp. parviflora is increasing, rather it had previously been overlooked.

The Plastid Genome of Deschampsia cespitosa (Poaceae)

Plastid genome analysis of non-model organisms provides valuable information for basic research e.g., molecular evolutionary genomics, phylogeny and phylogeography. Deschampsia cespitosa is the most widespread species of the genus and it is a common grass that is found across Eurasia and North America. Scattered populations in regions of appropriate ecological conditions are also found in Australia, New Zealand and southern South America, where it is sympatric with D. antarctica. We analyzed the plastid genome of a sample of Deschampsia cespitosa of the Austrian Alps using high-throughput sequencing. The plastid (cp) genome shows the typical quadripartite structure with a length of 135,340 bp, comprising a large single-copy (LSC) region of 79,992 bp, a small single-copy (SSC) region of 12,572 bp and two inverted repeats (IR) regions of 21,388 bp each. It contains 115 genes, including 85 protein-coding genes, four ribosomal RNA genes and 30 transfer RNA genes. The GC content (%), number of repeats and microsatellites, RNA editing sites and codon usage were highly similar to those of D. antarctica. The results of this present study highlight the extremely conserved nature of the cp genome in this group, since the comparison involved individuals separated by about 13,000 km, from the Alps to Antarctica.

Chelator production by Deschampsia cespitosa (L.) Beauv. in adaptive Ni/Cu hyper-tolerance derived from fields in the Sudbury region and lab assessment

Plants possess a complex network of mechanisms to utilize and, if necessary, detoxify metals. Plants utilize constitutive basal tolerance mechanisms to maintain appropriate internal metal levels under normal conditions. However, adaptive hyper-tolerance mechanisms are used in order to tolerate excess metal exposure. The production of metal binding chelators could be one way to convey these tolerances. Chelator production of field and greenhouse-derived materials was investigated to determine any multi-metal hyper-tolerances in different populations of the grass Deschampsia cespitosa (L.) Beauv. Plant tissue was collected from metal-contaminated mine sites, and from specimens grown in metal exposure hydroponic experiments. The chelator metabolites from these samples were simultaneously analyzed using HPLC-tandem mass spectrometry. In the hydroponic grown grass, histidine was produced at high concentrations solely in the hyper-tolerant populations during metal exposure. In all of the populations, the responses of chelators were metal-specific, where levels of nicotianamine were at high concentrations during Ni exposure, and levels of phytochelatins were high during Cu exposure. Moreover, a similar pattern of chelator production was seen in the root specimens collected from mine sites contaminated with Ni and (or) Cu. Histidine was the strongest Ni chelator involved in adaptive hyper-tolerance, while constitutive basal tolerance to Ni and Cu was observed via the responses of nicotianamine and phytochelatin, respectively.

The Influence of Plant Dominants on the Associated Species Abundance in Wet Tall-Herb Meadow Plant Communities

Plant interactions in wet tall-herb meadow plant communities were described through dominant and edificator species identification. Five dominant species were identified: Alopecurus pratensis, Filipendula ulmaria, Deschampsia cespitosa, Anthriscus sylvestris, and Angelica sylvestris. The effects of species were studied using ANOVA and correlation analyses. Not all dominants were recognised as edificators. Edificators (Alopecurus pratensis, Filipendula ulmaria, Deschampsia cespitosa, Angelica sylvestris) had a negative effect on the various abundance indicators of associated species: percent cover, number, phytomass, and height. The edificator effects differed significantly in their level and duration and depended on the biomorphs of dominants. The perennial species Alopecurus pratensis, Filipendula ulmaria, and Deschampsia cespitosa were strong constant edificators. Angelica sylvestris, a short-lived monocarpic from the Apiaceae, is a weak seasonal edificator, while Anthriscus sylvestris is not an edificator. Analysis of the life strategies of species showed that competitors are not always edificators (Anthriscus sylvestris), whereas stress-tolerant may show edificator properties (Deschampsia cespitosa). The associated plant species often show positive interactions. Most of the associated species are stress-tolerant and have not an edificator effect.