An Open Reading Frame Downstream ofRhizobium meliloti nodQ1Shows Nucleotide Sequence Similarity to anAgrobacterium tumefaciensInsertion Sequence

1994 ◽  
Vol 7 (1) ◽  
pp. 151 ◽  
Author(s):  
Julie Schwedock
Keyword(s):  
Nucleotide Sequence ◽  
Sequence Similarity ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Nucleotide Sequence Similarity ◽  
Ofrhizobium Meliloti

scholarly journals Complete Genome Sequence of a Novel Human Papillomavirus Isolated from Oral Rinse

2017 ◽  
Vol 5 (45) ◽  
Author(s):  
Zigui Chen ◽  
Martin C. S. Wong ◽  
Po Yee Wong ◽  
Wendy C. S. Ho ◽  
Miaoyin Liang ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Nucleotide Sequence ◽  
Complete Genome Sequence ◽  
Sequence Similarity ◽  
Species Group ◽  
Open Reading Frame ◽  
Male Resident ◽  
Reading Frame ◽  
Oral Rinse ◽  
Chinese Male

ABSTRACT A novel human papillomavirus (HPV TG550) isolated from the oral rinse of a Chinese male resident was fully characterized. The L1 open reading frame of HPV TG550 shares 82.5% nucleotide sequence similarity with its closest relative, HPV166, and clusters within the species group Gammapapillomavirus 19.

scholarly journals Cloning, Nucleotide Sequence, and Expression of the Gene Encoding the Bacteriocin Boticin B from Clostridium botulinum Strain 213B

2000 ◽  
Vol 66 (12) ◽  
pp. 5480-5483 ◽  
Author(s):  
Sean S. Dineen ◽  
Marite Bradshaw ◽  
Eric A. Johnson
Keyword(s):  
Amino Acids ◽  
Nucleotide Sequence ◽  
Clostridium Botulinum ◽  
Shuttle Vector ◽  
Open Reading Frame ◽  
Gene Encoding ◽  
Reading Frame ◽  
Heat Stable ◽  
Group I

ABSTRACT Boticin B is a heat-stable bacteriocin produced byClostridium botulinum strain 213B that has inhibitory activity against various strains of C. botulinum and related clostridia. The gene encoding the bacteriocin was localized to a 3.0-kb HindIII fragment of an 18.8-kb plasmid, cloned, and sequenced. DNA sequencing revealed the boticin B structural gene,btcB, to be an open reading frame encoding 50 amino acids. A C. botulinum strain 62A transconjugant containing theHindIII fragment inserted into a clostridial shuttle vector expressed boticin B, although at much lower levels than those observed in C. botulinum 213B. To our knowledge, this is the first demonstration and characterization of a bacteriocin from toxigenic group I C. botulinum.

Development of a Self-Cloning System for Actinomadura verrucosospora and Identification of Polyketide Synthase Genes Essential for Production of the Angucyclic Antibiotic Pradimicin

1999 ◽  
Vol 65 (6) ◽  
pp. 2703-2709 ◽  
Author(s):  
Tohru Dairi ◽  
Yoshimitsu Hamano ◽  
Tamotsu Furumai ◽  
Toshikazu Oki
Keyword(s):  
Nucleotide Sequence ◽  
Polyketide Synthase ◽  
Selectable Marker ◽  
Plasmid Vector ◽  
Open Reading Frame ◽  
Antibiotic Biosynthesis ◽  
Nucleotide Sequence Analysis ◽  
Reading Frame ◽  
Cloning System ◽  
Open Reading Frame 1

ABSTRACT A self-cloning system for Actinomadura verrucosospora, a producer of the angucyclic antibiotic pradimicin A (PRM A), has been developed. The system is based on reproducible and reliable protoplasting and regeneration conditions for A. verrucosospora and a novel plasmid vector that consists of a replicon from a newly found Actinomadura plasmid and a selectable marker cloned from the Actinomadurastrain. The system has an efficiency of more than 105CFU/microgram of DNA. Using this system, we have cloned and identified the polyketide synthase (PKS) genes essential for PRM A biosynthesis from A. verrucosospora. Nucleotide sequence analysis of the 3.5-kb SalI-SphI fragment showed that ketosynthase subunits (open reading frame 1 [ORF1] and ORF2) of the essential PKS genes have strong similarities (59 to 89%) to those for angucyclic antibiotic biosynthesis.

scholarly journals Identification of a Pneumococcal Glycosidase That Modifies O-Linked Glycans

2009 ◽  
Vol 77 (4) ◽  
pp. 1389-1396 ◽  
Author(s):  
Carolyn Marion ◽  
Dominique H. Limoli ◽  
Gregory S. Bobulsky ◽  
Jessica L. Abraham ◽  
Amanda M. Burnaugh ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Bifidobacterium Longum ◽  
Airway Epithelial Cells ◽  
Open Reading Frame ◽  
Glycan Structure ◽  
Dependent Manner ◽  
Upper Respiratory Tract ◽  
Reading Frame ◽  
Human Airway ◽  
Published Evidence

ABSTRACT Colonization of the airway by Streptococcus pneumoniae is typically asymptomatic; however, progression of bacteria beyond the oronasopharynx can cause diseases including otitis media and pneumonia. The mechanisms by which S. pneumoniae establishes and maintains colonization remain poorly understood. Both N-linked and O-linked glycans are abundant in the airway. Our previous research demonstrated that S. pneumoniae can sequentially deglycosylate N-linked glycans and suggested that this modification of sugar structures may aid in colonization. There is published evidence that S. pneumoniae expresses a secreted O-glycosidase that cleaves galactose β1-3 N-acetylgalactosamine (Galβ1-3GalNAc) from core-1 O-linked glycans; however, the biological function of this enzyme has not previously been determined. We established that the activity is not secreted but is instead surface associated in a sortase-dependent manner. Genome analysis revealed an open reading frame predicted to encode a sortase-dependent surface protein with sequence similarity to the O-glycosidase of Bifidobacterium longum. Deletion of this pneumococcal open reading frame confirmed that this gene encodes an O-glycosidase. Experiments using a model glycoconjugate demonstrated that this O-glycosidase, together with the neuraminidase NanA, is required for S. pneumoniae to cleave sialylated core-1 O-linked glycans. The ability of the O-glycosidase mutant to cleave this glycan structure was restored by both genetic complementation and the addition of O-glycosidase. The mutant showed a reduction in adherence to human airway epithelial cells and a significantly decreased ability to colonize the upper respiratory tract, suggesting that cleavage of core-1 O-linked glycans enhances the ability of S. pneumoniae to colonize the human airway.

Isolation of a RT-PCR fragment from human colon and sheep rumen RNA with nucleotide sequence similarity to human and rat urea transporter isoforms

1998 ◽  
Vol 26 (2) ◽  
pp. S122-S122 ◽  
Author(s):  
ARMIN RITZHAUPT ◽  
I. STUART WOOD ◽  
ALLAN A. JACKSON ◽  
BRENDAN J. MORAN ◽  
SORAYA P. SHIRAZI-BEECHEY
Keyword(s):  
Nucleotide Sequence ◽  
Sequence Similarity ◽  
Human Colon ◽  
Urea Transporter ◽  
Rt Pcr ◽  
Nucleotide Sequence Similarity ◽  
Sheep Rumen
Sign in / Sign up

Export Citation Format

Share Document