scholarly journals Complete Genome Sequence of a Novel Human Papillomavirus Isolated from Oral Rinse

2017 ◽  
Vol 5 (45) ◽  
Author(s):  
Zigui Chen ◽  
Martin C. S. Wong ◽  
Po Yee Wong ◽  
Wendy C. S. Ho ◽  
Miaoyin Liang ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Nucleotide Sequence ◽  
Complete Genome Sequence ◽  
Sequence Similarity ◽  
Species Group ◽  
Open Reading Frame ◽  
Male Resident ◽  
Reading Frame ◽  
Oral Rinse ◽  
Chinese Male

ABSTRACT A novel human papillomavirus (HPV TG550) isolated from the oral rinse of a Chinese male resident was fully characterized. The L1 open reading frame of HPV TG550 shares 82.5% nucleotide sequence similarity with its closest relative, HPV166, and clusters within the species group Gammapapillomavirus 19.

scholarly journals Complete Genome Sequence of a Novel Human Gammapapillomavirus Isolated from Skin

2017 ◽  
Vol 5 (34) ◽  
Author(s):  
Rosario N. Brancaccio ◽  
Alexis Robitaille ◽  
Sankhadeep Dutta ◽  
Dana E. Rollison ◽  
Nicole Fischer ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Next Generation Sequencing ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Open Reading Frame ◽  
Next Generation ◽  
Degenerate Pcr ◽  
Reading Frame ◽  
Generation Sequencing

ABSTRACT A novel human papillomavirus (HPV ICB1) was fully characterized from a skin swab by using a sensitive degenerate PCR protocol combined with next-generation sequencing. The L1 open reading frame of HPV ICB1 shares 70.54% nucleotide homology with its closest relative, HPV164, and thus constitutes a novel human gammapapillomavirus.

scholarly journals Complete Genome Sequence of a Novel Human Betapapillomavirus Isolated from a Skin Sample

2017 ◽  
Vol 5 (13) ◽  
Author(s):  
Sankhadeep Dutta ◽  
Alexis Robitaille ◽  
Dana E. Rollison ◽  
Massimo Tommasino ◽  
Tarik Gheit
Keyword(s):  
Human Papillomavirus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genetic Characterization ◽  
Open Reading Frame ◽  
Skin Sample ◽  
Reading Frame ◽  
Beta 2

ABSTRACT We report the genetic characterization of a new papillomavirus (HPV_MTS1) isolated and fully cloned from a skin swab. The L1 open reading frame of HPV_MTS1 was 85% identical to its closest human papillomavirus (HPV) type 80, which belongs to the species beta-2 of the genus Betapapillomavirus, hence qualifying it as a new HPV type.

scholarly journals Complete Genome Sequence of a Novel Human Gammapapillomavirus Isolated from a Cervical Swab in Luxembourg

2018 ◽  
Vol 6 (11) ◽  
Author(s):  
Ardashel Latsuzbaia ◽  
Marc Arbyn ◽  
Sankhadeep Dutta ◽  
Marc Fischer ◽  
Tarik Gheit ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Next Generation Sequencing ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Cervical Swab ◽  
Generation Sequencing ◽  
Swab Specimen

ABSTRACT A novel human papillomavirus genotype was detected in a cervical swab specimen by next-generation sequencing after rolling circular amplification. It was fully cloned and characterized. The L1 open reading frame showed 77% nucleotide similarity with the closest genotype, HPV101, belonging to the gamma-6 species.

scholarly journals Cloning, Nucleotide Sequence, and Expression of the Gene Encoding the Bacteriocin Boticin B from Clostridium botulinum Strain 213B

2000 ◽  
Vol 66 (12) ◽  
pp. 5480-5483 ◽  
Author(s):  
Sean S. Dineen ◽  
Marite Bradshaw ◽  
Eric A. Johnson
Keyword(s):  
Amino Acids ◽  
Nucleotide Sequence ◽  
Clostridium Botulinum ◽  
Shuttle Vector ◽  
Open Reading Frame ◽  
Gene Encoding ◽  
Reading Frame ◽  
Heat Stable ◽  
Group I

ABSTRACT Boticin B is a heat-stable bacteriocin produced byClostridium botulinum strain 213B that has inhibitory activity against various strains of C. botulinum and related clostridia. The gene encoding the bacteriocin was localized to a 3.0-kb HindIII fragment of an 18.8-kb plasmid, cloned, and sequenced. DNA sequencing revealed the boticin B structural gene,btcB, to be an open reading frame encoding 50 amino acids. A C. botulinum strain 62A transconjugant containing theHindIII fragment inserted into a clostridial shuttle vector expressed boticin B, although at much lower levels than those observed in C. botulinum 213B. To our knowledge, this is the first demonstration and characterization of a bacteriocin from toxigenic group I C. botulinum.

Development of a Self-Cloning System for Actinomadura verrucosospora and Identification of Polyketide Synthase Genes Essential for Production of the Angucyclic Antibiotic Pradimicin

1999 ◽  
Vol 65 (6) ◽  
pp. 2703-2709 ◽  
Author(s):  
Tohru Dairi ◽  
Yoshimitsu Hamano ◽  
Tamotsu Furumai ◽  
Toshikazu Oki
Keyword(s):  
Nucleotide Sequence ◽  
Polyketide Synthase ◽  
Selectable Marker ◽  
Plasmid Vector ◽  
Open Reading Frame ◽  
Antibiotic Biosynthesis ◽  
Nucleotide Sequence Analysis ◽  
Reading Frame ◽  
Cloning System ◽  
Open Reading Frame 1

ABSTRACT A self-cloning system for Actinomadura verrucosospora, a producer of the angucyclic antibiotic pradimicin A (PRM A), has been developed. The system is based on reproducible and reliable protoplasting and regeneration conditions for A. verrucosospora and a novel plasmid vector that consists of a replicon from a newly found Actinomadura plasmid and a selectable marker cloned from the Actinomadurastrain. The system has an efficiency of more than 105CFU/microgram of DNA. Using this system, we have cloned and identified the polyketide synthase (PKS) genes essential for PRM A biosynthesis from A. verrucosospora. Nucleotide sequence analysis of the 3.5-kb SalI-SphI fragment showed that ketosynthase subunits (open reading frame 1 [ORF1] and ORF2) of the essential PKS genes have strong similarities (59 to 89%) to those for angucyclic antibiotic biosynthesis.

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