Grapevine Fanleaf Nepovirus P38 Putative Movement Protein Is Located on TubulesIn Vivo

AbstractNucleotide sequence of full-length cDNA ofpeanut stunt virus(PSV) Mi strain RNA3 was determined and compared with those of PSV-ER and -J (subgroup I) and PSV-W (subgroup II), strains ofcucumber mosaic virus(CMV) andtomato aspermy virus(TAV). PSV-Mi RNA3 consists of 2170 nt and has two open reading frames, encoding a putative movement protein (3a protein) and a coat protein (CP). PSV-Mi RNA3 is 77.7% and 78.5% identical to those of PSV-ER and -J, whereas it shares 76.6% identity with PSV-W. Nucleotide identity of3aandcpgenes between PSV strains Mi and ER, J and W was 78.3–79.3% and 74.4–77.8%, respectively. Amino acid identity of 3a and CP between PSV-Mi and -ER, -J and -W was 73.9–77.4% and 64.8–77.5%, respectively. RNA3 of PSV-Mi (GenBank accession no. AY775057) had a varied intercistronic and 5′-untranslated region compared with those of PSV strains ER, J and W. Results indicate that PSV-Mi represents a new PSV subgroup from China, designated as subgroup III.

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Pineapple bacilliform CO virus: Diversity, Detection, Distribution, and Transmission

Plant Disease ◽

10.1094/pdis-08-11-0718-re ◽

2012 ◽

Vol 96 (12) ◽

pp. 1798-1804 ◽

Cited By ~ 13

Author(s):

D. M. Sether ◽

M. J. Melzer ◽

W. B. Borth ◽

J. S. Hu

Keyword(s):

Reverse Transcriptase ◽

Movement Protein ◽

Rnase H ◽

Open Reading Frames ◽

Ananas Comosus ◽

Genomic Variants ◽

Virus Diversity ◽

Putative Movement Protein ◽

Dysmicoccus Neobrevipes ◽

Reading Frames

Members of the genus Badnavirus (family Caulimovirdae) have been identified in dicots and monocots worldwide. The genome of a pineapple badnavirus, designated Pineapple bacilliform CO virus-HI1 (PBCOV-HI1), and nine genomic variants (A through H) were isolated and sequenced from pineapple, Ananas comosus, in Hawaii. The 7,451-nucleotide genome of PBCOV-HI1 possesses three open reading frames (ORFs) encoding putative proteins of 20 (ORF1), 15 (ORF2), and 211 (ORF3) kDa. ORF3 encodes a polyprotein that includes a putative movement protein and viral aspartyl proteinase, reverse transcriptase, and RNase H regions. Three distinct groups of putative endogenous pineapple pararetroviral sequences and Metaviridae-like retrotransposons encoding long terminal repeat, reverse-transcriptase, RNase H, and integrase regions were also identified from the pineapple genome. Detection assays were developed to distinguish PBCOV-HI1 and genomic variants, putative endogenous pararetrovirus sequences, and Ananas Metaviridae sequences also identified in pineapple. PBCOV-HI1 incidences in two commercially grown pineapple hybrids, PRI 73-114 and PRI 73-50, was 34 to 68%. PBCOV-HI1 was transmitted by gray pineapple mealybugs, Dysmicoccus neobrevipes, to pineapple.

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In VivoDetection, RNA-Binding Properties and Characterization of the RNA-Binding Domain of the p7 Putative Movement Protein from Carnation Mottle Carmovirus (CarMV)

Virology ◽

10.1006/viro.1998.9596 ◽

1999 ◽

Vol 255 (2) ◽

pp. 354-365 ◽

Cited By ~ 48

Author(s):

Jose F. Marcos ◽

Marçal Vilar ◽

Enrique Pérez-Payá ◽

Vicente Pallás

Keyword(s):

Rna Binding ◽

Movement Protein ◽

Binding Domain ◽

Binding Properties ◽

Properties And Characterization ◽

Putative Movement Protein ◽

Rna Binding Domain

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Subcellular localization and in vivo identification of the putative movement protein of olive latent virus 2.

Journal of General Virology ◽

10.1099/0022-1317-80-5-1103 ◽

1999 ◽

Vol 80 (5) ◽

pp. 1103-1109 ◽

Cited By ~ 27

Author(s):

F Grieco ◽

M A Castellano ◽

G P Di Sansebastiano ◽

G Maggipinto ◽

J M Neuhaus ◽

...

Keyword(s):

Subcellular Localization ◽

Movement Protein ◽

Latent Virus ◽

Putative Movement Protein

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Serological Detection of Grapevine Virus A Using Antiserum to a Nonstructural Protein, the Putative Movement Protein

Phytopathology ◽

10.1094/phyto.1997.87.10.1041 ◽

1997 ◽

Vol 87 (10) ◽

pp. 1041-1045 ◽

Cited By ~ 29

Author(s):

E. Rubinson ◽

N. Galiakparov ◽

S. Radian ◽

I. Sela ◽

E. Tanne ◽

...

Keyword(s):

Movement Protein ◽

Early Stage ◽

Nonstructural Protein ◽

Effective Means ◽

Immunoblot Analysis ◽

Enzyme Linked Immunosorbent Assay ◽

Grapevine Virus ◽

Serological Detection ◽

Grapevine Virus A ◽

Putative Movement Protein

Grapevine virus A (GVA) is implicated in the etiology of the rugose wood disease. The coat protein (CP) and the putative movement protein (MP) genes of GVA were cloned and expressed in Escherichia coli and used to produce antisera. Both the CP and the MP were detected with their corresponding antisera in GVA-infected Nicotiana benthamiana. The MP was first detected at an early stage of the infection, 6 to 12 h after inoculation, and the CP was detected 2 to 3 days after inoculation. The CP and MP were detected by immunoblot analysis in rugose wood-affected grapevines. The MP could be detected in GVA-infected grapevines that tested negative for CP, both with CP antiserum and with a commercially available enzyme-linked immunosorbent assay kit. The study shows that detection of the nonstructural MP may be an effective means for serological detection of GVA infection in grapevines.

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In SituLocalization of the Putative Movement Protein (pr17) from Potato Leafroll Luteovirus (PLRV) in Infected and Transgenic Potato Plants

Virology ◽

10.1006/viro.1997.8679 ◽

1997 ◽

Vol 235 (2) ◽

pp. 311-322 ◽

Cited By ~ 53

Author(s):

J. Schmitz ◽

C. Stussi-Garaud ◽

E. Tacke ◽

D. Prüfer ◽

W. Rohde ◽

...

Keyword(s):

Movement Protein ◽

Transgenic Potato ◽

Potato Plants ◽

Transgenic Potato Plants ◽

Putative Movement Protein ◽

Potato Leafroll Luteovirus

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Two Novel Negative-Sense RNA Viruses Infecting Grapevine Are Members of a Newly Proposed Genus within the Family Phenuiviridae

Viruses ◽

10.3390/v11080685 ◽

2019 ◽

Vol 11 (8) ◽

pp. 685 ◽

Cited By ~ 7

Author(s):

Alfredo Diaz-Lara ◽

Beatriz Navarro ◽

Francesco Di Serio ◽

Kristian Stevens ◽

Min Sook Hwang ◽

...

Keyword(s):

Nucleocapsid Protein ◽

High Throughput Sequencing ◽

Movement Protein ◽

Rna Viruses ◽

Rt Pcr ◽

Rna Dependent Rna Polymerase ◽

Sequence Identity ◽

The Family ◽

Putative Movement Protein ◽

Negative Sense

Two novel negative-stranded (ns)RNA viruses were identified by high throughput sequencing in grapevine. The genomes of both viruses, named grapevine Muscat rose virus (GMRV) and grapevine Garan dmak virus (GGDV), comprise three segments with each containing a unique gene. Based on sequence identity and presence of typical domains/motifs, the proteins encoded by the two viruses were predicted to be: RNA-dependent RNA polymerase (RdRp), nucleocapsid protein (NP), and putative movement protein (MP). These proteins showed the highest identities with orthologs in the recently discovered apple rubbery wood viruses 1 and 2, members of a tentative genus (Rubodvirus) within the family Phenuiviridae. The three segments of GMRV and GGDV share almost identical sequences at their 5′ and 3′ termini, which are also complementary to each other and may form a panhandle structure. Phylogenetics based on RdRp, NP and MP placed GMRV and GGDV in the same cluster with rubodviruses. Grapevine collections were screened for the presence of both novel viruses via RT-PCR, identifying infected plants. GMRV and GGDV were successfully graft-transmitted, thus, they are the first nsRNA viruses identified and transmitted in grapevine. Lastly, different evolutionary scenarios of nsRNA viruses are discussed.

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