Pc4, a putative movement protein of Rice stripe virus, interacts with a type I DnaJ protein and a small Hsp of rice

Virus Genes ◽  
2009 ◽  
Vol 38 (2) ◽  
pp. 320-327 ◽  
Author(s):  
Lianming Lu ◽  
Zhenguo Du ◽  
Meiling Qin ◽  
Ping Wang ◽  
Hanhong Lan ◽  
...  
Keyword(s):  
Movement Protein ◽  
Rice Stripe Virus ◽  
Type I ◽  
Dnaj Protein ◽  
Small Hsp ◽  
Putative Movement Protein

scholarly journals NSvc4 Encoded by Rice Stripe Virus Targets Host Chloroplasts to Suppress Chloroplast-Mediated Defense

Viruses ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 36
Author(s):  
Zongdi Li ◽  
Chenyang Li ◽  
Shuai Fu ◽  
Yu Liu ◽  
Yi Xu ◽  
...  
Keyword(s):  
Nicotiana Benthamiana ◽  
Actin Filaments ◽  
Movement Protein ◽  
Infectious Clone ◽  
Rice Stripe Virus ◽  
Potato Virus X ◽  
Host Factors ◽  
Ros Production ◽  
Rsv Infection ◽  
Detailed Function

Our previous research found that NSvc4, the movement protein of rice stripe virus (RSV), could localize to the actin filaments, endoplasmic reticulum, plasmodesmata, and chloroplast, but the roles of NSvc4 played in the chloroplast were opaque. Here, we confirm the accumulation of NSvc4 in the chloroplasts and the N-terminal 1–73 amino acids of NSvc4 are sufficient to localize to chloroplasts. We provide evidence to show that chloroplast-localized NSvc4 can impair the chloroplast-mediated immunity. Expressing NSvc4 in Nicotiana benthamiana leaves results in the decreased expression of defense-related genes NbPR1, NbPR2, and NbWRKY12 and the inhibition of chloroplast-derived ROS production. In addition, generation of an infectious clone of potato virus X (PVX) carrying NSvc4 facilitates PVX infection in N. benthamiana plants. Moreover, we identify two chloroplast-related host factors, named NbGAPDH-A and NbPsbQ1, both of which can interact with NSvc4. Knockdown of NbGAPDH-A or NbPsbQ1 can both promote RSV infection. Our results decipher a detailed function of NSvc4 in the chloroplast.

RNA3 nucleotide sequence analyses ofpeanut stunt virusMi strain

2005 ◽  
Vol 2 (1) ◽  
pp. 33-38
Author(s):  
Xu Ze-Yong ◽  
Yan Li-Ying ◽  
Chen Kun-Rong ◽  
Marcel Prins
Keyword(s):  
Nucleotide Sequence ◽  
Movement Protein ◽  
Amino Acid Identity ◽  
Open Reading Frames ◽  
Sequence Analyses ◽  
Tomato Aspermy Virus ◽  
Subgroup Ii ◽  
Putative Movement Protein ◽  
Peanut Stunt Virus ◽  
Reading Frames

AbstractNucleotide sequence of full-length cDNA ofpeanut stunt virus(PSV) Mi strain RNA3 was determined and compared with those of PSV-ER and -J (subgroup I) and PSV-W (subgroup II), strains ofcucumber mosaic virus(CMV) andtomato aspermy virus(TAV). PSV-Mi RNA3 consists of 2170 nt and has two open reading frames, encoding a putative movement protein (3a protein) and a coat protein (CP). PSV-Mi RNA3 is 77.7% and 78.5% identical to those of PSV-ER and -J, whereas it shares 76.6% identity with PSV-W. Nucleotide identity of3aandcpgenes between PSV strains Mi and ER, J and W was 78.3–79.3% and 74.4–77.8%, respectively. Amino acid identity of 3a and CP between PSV-Mi and -ER, -J and -W was 73.9–77.4% and 64.8–77.5%, respectively. RNA3 of PSV-Mi (GenBank accession no. AY775057) had a varied intercistronic and 5′-untranslated region compared with those of PSV strains ER, J and W. Results indicate that PSV-Mi represents a new PSV subgroup from China, designated as subgroup III.

scholarly journals Pineapple bacilliform CO virus: Diversity, Detection, Distribution, and Transmission

Plant Disease ◽  
2012 ◽  
Vol 96 (12) ◽  
pp. 1798-1804 ◽  
Author(s):  
D. M. Sether ◽  
M. J. Melzer ◽  
W. B. Borth ◽  
J. S. Hu
Keyword(s):  
Reverse Transcriptase ◽  
Movement Protein ◽  
Rnase H ◽  
Open Reading Frames ◽  
Ananas Comosus ◽  
Genomic Variants ◽  
Virus Diversity ◽  
Putative Movement Protein ◽  
Dysmicoccus Neobrevipes ◽  
Reading Frames

Members of the genus Badnavirus (family Caulimovirdae) have been identified in dicots and monocots worldwide. The genome of a pineapple badnavirus, designated Pineapple bacilliform CO virus-HI1 (PBCOV-HI1), and nine genomic variants (A through H) were isolated and sequenced from pineapple, Ananas comosus, in Hawaii. The 7,451-nucleotide genome of PBCOV-HI1 possesses three open reading frames (ORFs) encoding putative proteins of 20 (ORF1), 15 (ORF2), and 211 (ORF3) kDa. ORF3 encodes a polyprotein that includes a putative movement protein and viral aspartyl proteinase, reverse transcriptase, and RNase H regions. Three distinct groups of putative endogenous pineapple pararetroviral sequences and Metaviridae-like retrotransposons encoding long terminal repeat, reverse-transcriptase, RNase H, and integrase regions were also identified from the pineapple genome. Detection assays were developed to distinguish PBCOV-HI1 and genomic variants, putative endogenous pararetrovirus sequences, and Ananas Metaviridae sequences also identified in pineapple. PBCOV-HI1 incidences in two commercially grown pineapple hybrids, PRI 73-114 and PRI 73-50, was 34 to 68%. PBCOV-HI1 was transmitted by gray pineapple mealybugs, Dysmicoccus neobrevipes, to pineapple.

scholarly journals Subcellular localization and in vivo identification of the putative movement protein of olive latent virus 2.

1999 ◽  
Vol 80 (5) ◽  
pp. 1103-1109 ◽  
Author(s):  
F Grieco ◽  
M A Castellano ◽  
G P Di Sansebastiano ◽  
G Maggipinto ◽  
J M Neuhaus ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Movement Protein ◽  
Latent Virus ◽  
Putative Movement Protein

A transmembrane domain determines the localization of rice stripe virus pc4 to plasmodesmata and is essential for its function as a movement protein

2014 ◽  
Vol 183 ◽  
pp. 112-116 ◽  
Author(s):  
Lingling Rong ◽  
Yuwen Lu ◽  
Lin Lin ◽  
Hongying Zheng ◽  
Fei Yan ◽  
...  
Keyword(s):  
Transmembrane Domain ◽  
Movement Protein ◽  
Rice Stripe Virus

scholarly journals Serological Detection of Grapevine Virus A Using Antiserum to a Nonstructural Protein, the Putative Movement Protein

1997 ◽  
Vol 87 (10) ◽  
pp. 1041-1045 ◽  
Author(s):  
E. Rubinson ◽  
N. Galiakparov ◽  
S. Radian ◽  
I. Sela ◽  
E. Tanne ◽  
...  
Keyword(s):  
Movement Protein ◽  
Early Stage ◽  
Nonstructural Protein ◽  
Effective Means ◽  
Immunoblot Analysis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Grapevine Virus ◽  
Serological Detection ◽  
Grapevine Virus A ◽  
Putative Movement Protein

Grapevine virus A (GVA) is implicated in the etiology of the rugose wood disease. The coat protein (CP) and the putative movement protein (MP) genes of GVA were cloned and expressed in Escherichia coli and used to produce antisera. Both the CP and the MP were detected with their corresponding antisera in GVA-infected Nicotiana benthamiana. The MP was first detected at an early stage of the infection, 6 to 12 h after inoculation, and the CP was detected 2 to 3 days after inoculation. The CP and MP were detected by immunoblot analysis in rugose wood-affected grapevines. The MP could be detected in GVA-infected grapevines that tested negative for CP, both with CP antiserum and with a commercially available enzyme-linked immunosorbent assay kit. The study shows that detection of the nonstructural MP may be an effective means for serological detection of GVA infection in grapevines.

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