scholarly journals Transcription of a Gene Encoding a Lectinlike Glycoprotein Is Induced in Root Cells Harboring Arbuscular Mycorrhizal Fungi in Pisum sativum

1999 ◽  
Vol 12 (9) ◽  
pp. 785-791 ◽  
Author(s):  
Raffaella Balestrini ◽  
Silvia Perotto ◽  
Elena Gasverde ◽  
Preeti Dahiya ◽  
Lise-Lotte Guldmann ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Mycorrhizal Fungi ◽  
Root Nodules ◽  
Arbuscular Mycorrhizal ◽  
Pcr Primers ◽  
Root Cells ◽  
Hybridization Probes ◽  
Mycorrhizal Roots ◽  
Pea Roots

The gene PsNlec1, which encodes a lectinlike glycoprotein, is strongly expressed in pea nodule tissue. Using gene-specific polymerase chain reaction (PCR) primers, in situ hybridization probes, and specific antisera derived from the PsNlec1 sequence, we investigated gene expression associated with the arbuscular mycorrhizal (AM) symbiosis of pea roots. With the use of reverse transcription (RT)-PCR and cold in situ hybridization, strong expression of the transcript was demonstrated not only in root nodules but also in mycorrhizal roots when the cells were colonized by the endomycorrhizal fungus Glomus versiforme. No transcript was detectable in uninfected pea roots. With an antiserum raised against PsNLEC-1 polypeptide, a single antigenic band (25 kDa) was observed following gel electrophoresis of extracts from mycorrhizal roots. However, the amount of antigen was apparently too low to be detected by immunogold localization in tissue sections of pea mycorrhizal roots.

scholarly journals OligoMinerApp: a web-server application for the design of genome-scale oligonucleotide in situ hybridization probes through the flexible OligoMiner environment

2020 ◽  
Vol 48 (W1) ◽  
pp. W332-W339
Author(s):  
Marco Passaro ◽  
Martina Martinovic ◽  
Valeria Bevilacqua ◽  
Elliot A Hershberg ◽  
Grazisa Rossetti ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Technology Development ◽  
Supervised Machine Learning ◽  
Web Based ◽  
Hybridization Probes ◽  
Cutting Out ◽  
Novel Method ◽  
Genome Scale ◽  
Server Application

Abstract Fluorescence in situ hybridization (FISH) is a powerful single-cell technique that harnesses nucleic acid base pairing to detect the abundance and positioning of cellular RNA and DNA molecules in fixed samples. Recent technology development has paved the way to the construction of FISH probes entirely from synthetic oligonucleotides (oligos), allowing the optimization of thermodynamic properties together with the opportunity to design probes against any sequenced genome. However, comparatively little progress has been made in the development of computational tools to facilitate the oligos design, and even less has been done to extend their accessibility. OligoMiner is an open-source and modular pipeline written in Python that introduces a novel method of assessing probe specificity that employs supervised machine learning to predict probe binding specificity from genome-scale sequence alignment information. However, its use is restricted to only those people who are confident with command line interfaces because it lacks a Graphical User Interface (GUI), potentially cutting out many researchers from this technology. Here, we present OligoMinerApp (http://oligominerapp.org), a web-based application that aims to extend the OligoMiner framework through the implementation of a smart and easy-to-use GUI and the introduction of new functionalities specially designed to make effective probe mining available to everyone.

scholarly journals Rhizobia associated with neotropical tree Centrolobium tomentosum used in riparian restoration

2008 ◽  
Vol 54 (No. 11) ◽  
pp. 498-508 ◽  
Author(s):  
M.C. Pagano
Keyword(s):  
Mycorrhizal Fungi ◽  
Random Amplified Polymorphic Dna ◽  
Arbuscular Mycorrhizal ◽  
Antibiotics Resistance ◽  
Fast Growing ◽  
Nursery Culture ◽  
Mixed Inoculum ◽  
Neotropical Tree ◽  
Molecular Relationships

<I>Centrolobium tomentosum</I> is a tropical legume tree indicated for functional and structural restoration of riparian areas. This study was conducted to characterize the rhizobia isolated from nodules of <I>C. tomentosum in situ</I> and to determine their capacity of renodulation, in an experimental area of land rehabilitation in the Rio Doce valley. Nodulation potential to inoculation with 2 selected fast-growing <I>Rhizobium</I> strains separately and a mixed inoculum of arbuscular mycorrhizal fungi was evaluated by the use of antibiotics resistance. Flood disturbance were observed not to affect renodulation by fast-growing strains. DNA fingerprinting RAPD (random amplified polymorphic DNA) and lipopolysaccharides (LPS) profiles were used to examine molecular relationships among field isolates, inoculants and reference strains. Maximal renodulation was exhibited by strain BHCBAb1 after 24 months after transplantation. <I>Centrolobium tomentosum</I> forms symbiosis with fast- and slow-growing <I>Rhizobium</I> strains, and it is suggested that their nursery culture could be improved by inoculation of selected strain under low nitrogen-input conditions.

scholarly journals Angiosperm Gymnostoma trees produce root nodules colonized by arbuscular mycorrhizal fungi related to Glomus

2001 ◽  
Vol 149 (1) ◽  
pp. 115-125 ◽  
Author(s):  
E. Duhoux ◽  
G. Rinaudo ◽  
H. G. Diem ◽  
F. Auguy ◽  
D. Fernandez ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Root Nodules ◽  
Arbuscular Mycorrhizal

Spatial monitoring of gene activity in extraradical and intraradical developmental stages of arbuscular mycorrhizal fungi by direct fluorescent in situ RT-PCR

2008 ◽  
Vol 45 (8) ◽  
pp. 1155-1165 ◽  
Author(s):  
Pascale M.A. Seddas ◽  
Christine Arnould ◽  
Marie Tollot ◽  
Cecilia M. Arias ◽  
Vivienne Gianinazzi-Pearson
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Developmental Stages ◽  
Arbuscular Mycorrhizal ◽  
Gene Activity ◽  
Rt Pcr
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