Xiphinemaspp. Associated with Tomato Ringspot Virus Infection of Pennsylvania Fruit Crops

Tissue samples from 15 woody and herbaceous plant species, including white ash (Fraxinusamericana L.), were collected monthly during the spring and summer of 1985 and indexed by ELISA for tomato ringspot virus. The virus was detected in 12 of 23 white ash indexed, most frequently in the roots. Of 88 white ash seedlings inoculated with purified tomato ringspot virus, the virus was detected by ELISA in 7 seedlings after two cycles of growth and dormancy, and in only 1 seedling after one cycle of growth and dormancy postinoculation. The virus was also detected in Fragariavirginiana Mill., Prunusvirginiana L., Rubusoccidentalis L., R. strigosus Michx., Solidagocanadensis L., Trifoliumpratense L., T. repens L., and Daucuscarota L. growing within 50 m of infected ash.

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Nucleotide sequence of tomato ringspot virus RNA1

Journal of General Virology ◽

10.1099/0022-1317-76-2-465 ◽

1995 ◽

Vol 76 (2) ◽

pp. 465-473 ◽

Cited By ~ 44

Author(s):

M. E. Rott ◽

A. Gilchrist ◽

L. Lee ◽

D. Rochon

Keyword(s):

Nucleotide Sequence ◽

Ringspot Virus ◽

Tomato Ringspot Virus

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Grapevine yellow speckle — a newly recognized graft-transmissible disease of Vitis

Australian Journal of Agricultural Research ◽

10.1071/ar9720447 ◽

1972 ◽

Vol 23 (3) ◽

pp. 447 ◽

Cited By ~ 15

Author(s):

RH Taylor ◽

RC Woodham

Keyword(s):

Seed Transmission ◽

Ringspot Virus ◽

Herbaceous Plants ◽

Grapevine Fanleaf Virus ◽

The Past ◽

Tomato Ringspot Virus ◽

Wide Range ◽

Heat Therapy ◽

Transmissible Disease ◽

Leaf Symptoms

Evidence is presented to show that many grapevines showing leaf symptoms which, particularly when intense, cannot be distinguished from those caused by strains of grapevine fanleaf virus (GFV) or tomato ringspot virus (TRSV) are infected with a previously undescribed disease for which the name grapevine yellow speckle (GYS) is proposed. The causal agent of the disease has not been isolated, but it is graft-transmissible from infected to healthy grapevines. It has not been transmitted by sap inoculation to herbaceous plants. The disease has been detected in some grapevine cultivars grown in Australia for many years, and also in several other cultivars and rootstocks imported from California over the past 10 years. There is no evidence of natural spread or of seed transmission. Attempts to eliminate GYS from plants by heat therapy and propagation of tip cuttings have been unsuccessful. Mataro and LN33, the best indicators for GYS at present, are not entirely satisfactory and there is a need to find an indicator which will detect GYS in a wide range of environments. The disease has not been previously detected, probably because of its similarity to GFV and TRSV and its peculiarity in expressing symptoms only in some environments.

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A Survey for Nine Major Viruses of Grapevines in Tennessee Vineyards

Plant Health Progress ◽

10.1094/php-03-20-0018-rs ◽

2020 ◽

Vol 21 (3) ◽

pp. 157-161

Author(s):

Nourolah Soltani ◽

Rongbin Hu ◽

Darrell D. Hensley ◽

David L. Lockwood ◽

Keith. L. Perry ◽

...

Keyword(s):

First Record ◽

Ringspot Virus ◽

Rt Pcr ◽

Grapevine Fanleaf Virus ◽

Arabis Mosaic Virus ◽

Growing Seasons ◽

Tomato Ringspot Virus ◽

The Status ◽

Diagnostic Approaches ◽

Das Elisa

Despite the significance of grape production to the fruit industry in Tennessee (TN), no published information has been available on viruses affecting grapevines in the state. Hence, a survey was conducted during the 2016 and 2017 growing seasons to determine the status of nine major viruses of grapevines in TN vineyards by taking advantage of classical serological assays and confirmatory nucleic acid-based diagnostic approaches. A total of 349 samples from 23 grapevine cultivars mostly displaying viral-like symptoms were collected from 23 commercial vineyards. All samples were assayed by DAS-ELISA for arabis mosaic virus (ArMV), grapevine leafroll-associated virus (GLRaV)-1, GLRaV-2, GLRaV-3, GLRaV-4, grapevine fanleaf virus (GFLV), tobacco ringspot virus (TRSV), and tomato ringspot virus (ToRSV). Selected serologically positive samples were also tested by RT-PCR, followed by Sanger sequencing of the generated amplicons. Additionally, 19 grapevines displaying symptoms characteristic of grapevine red blotch virus (GRBV) were also assayed by PCR followed by confirmatory sequencing-based methods. Collectively, these assays verifiably detected GLRaV-1, GLRaV-2, GLRaV-3, ToRSV, and GRBV in TN vineyards. This is the first record of the presence of these viruses in TN vineyards. ArMV, GLRaV-4, GFLV, and TRSV were not detected. The majority of samples tested positive for a single virus, whereas mixed infections with more than one virus were detected in 37% of samples.

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