scholarly journals Potyvirus Complexes in Sweetpotato: Occurrence in Australia, Serological and Molecular Resolution, and Analysis of the Sweet potato virus 2 (SPV2) Component

Plant Disease ◽  
2006 ◽  
Vol 90 (9) ◽  
pp. 1120-1128 ◽  
Author(s):  
Fred Tairo ◽  
Roger A. C. Jones ◽  
Jari P. T. Valkonen
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Restriction Analysis ◽  
Degenerate Primers ◽  
Protein Encoding ◽  
Tentative Member ◽  
Sweet Potato Virus ◽  
Polymerase Chain ◽  
Primer Polymerase Chain Reaction ◽  
First Time

A survey for viruses in sweetpotato revealed the presence of Sweet potato virus 2 (SPV2; synonymous to Sweet potato virus Y and Ipomoea vein mosaic virus), a tentative member of the genus Potyvirus, for the first time in Australia. The SPV2-infected sweetpotato plants were also infected with strains RC and/or C of Sweet potato feathery mottle virus (SPFMV; genus Potyvirus). Five SPV2 and SPFMV isolates from Australia were sequence-characterized at the 3′ - proximal end (ca. 1.8 kb) of the genome. A simple and sensitive diagnostic procedure was devised to readily differentiate SPV2 and the two strains of SPFMV from sweetpotato plants that contained these viruses in complexes. The method involved reverse transcription with oligoT25 primer, polymerase chain reaction using a combination of degenerate primers, and restriction analysis of the 1.8-kb amplification products with HindIII and PvuII endonucleases. The Nproximal 543 nucleotides of the SPV2 coat protein-encoding sequence of the Australian isolates and 14 other isolates from Asia, Africa, Europe, and North America were subjected to phylogenetic analysis. The Australian SPV2 isolates formed a separate clade that was closest to a clade containing two North American isolates.

scholarly journals Species and genetic variability of sweet potato viruses in China

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Yongjiang Wang ◽  
Yanhong Qin ◽  
Shuang Wang ◽  
Desheng Zhang ◽  
Yuting Tian ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Sweet Potato ◽  
Potato Virus ◽  
Full Length ◽  
Genomic Sequences ◽  
Potato Leaf ◽  
Potato Viruses ◽  
Sweet Potato Virus ◽  
First Time ◽  
Leaf Curl

AbstractChina is the world’s largest producer of sweet potato (Ipomoea batatas (L.) Lam.). Considering that there are numerous sweet potato-producing regions in China and sweet potato is a vegetatively propagated crop, the genetic diversity of sweet potato viruses could be high in the country. However, studies on species and genetic variabilities of sweet potato viruses in China are limited, making it difficult to prevent and control viral diseases in this crop. During 2014–2019, sweet potato samples with viral disease-like symptoms were randomly collected from sweet potato fields in 25 provinces in China. Twenty-one virus species, including 12 DNA and 9 RNA viruses, were identified in the samples using next-generation sequencing, polymerase chain reaction and rolling-circle amplification methods. One novel sweepovirus species, Sweet potato leaf curl Hubei virus (SPLCHbV), was identified. Two species, Sweet potato collusive virus and Tobacco mosaic virus, were identified for the first time in sweet potato in China. Full-length or nearly full-length genomic sequences of 111 isolates belonging to 18 viral species were obtained. Genome sequence comparisons of potyvirus isolates obtained in this study indicate that the genome of sweet potato virus 2 is highly conserved, whereas the other four potyviruses, sweet potato feathery mottle virus, sweet potato virus G, sweet potato latent virus and sweet potato virus C, exhibited a high genetic variability. The similarities among the 40 sweepovirus genomic sequences obtained from eight sweepovirus species are 67.0–99.8%. The eight sweepoviruses include 14 strains, of which 4 novel strains were identified from SPLCHbV and 1 from sweet potato leaf curl Guangxi virus. Five sweet potato chlorotic stunt virus (SPCSV) isolates obtained belong to the WA strain, and the genome sequences of SPCSV are highly conserved. Together, this study for the first time comprehensively reports the variability of sweet potato viruses in China.

Control strategies for sweet potato virus disease in Africa

2004 ◽  
Vol 100 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Richard W. Gibson ◽  
Valentine Aritua ◽  
Emmanuel Byamukama ◽  
Isaac Mpembe ◽  
James Kayongo
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Virus Disease ◽  
Control Strategies ◽  
Potato Virus Disease ◽  
Sweet Potato Virus ◽  
Sweet Potato Virus Disease

scholarly journals Occurrence and Molecular Characterization of Recombinant Potato virus YNTN Isolates from Sulawesi, Indonesia

Plant Disease ◽  
2016 ◽  
Vol 100 (2) ◽  
pp. 269-275 ◽  
Author(s):  
Mohamad Chikh-Ali ◽  
Nilsa A. Bosque-Pérez ◽  
Dalton Vander Pol ◽  
Dantje Sembel ◽  
Alexander V. Karasev
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Cultivar ◽  
Whole Genome ◽  
Chain Reaction ◽  
Recombinant Type ◽  
Whole Genomes ◽  
Polymerase Chain ◽  
First Time

The importance of potato has increased dramatically in Indonesia over the last three decades. During this period, ‘Granola’, a potato cultivar originally from Germany, has become the most common cultivar for fresh consumption in Indonesia. In August 2014, a survey was conducted in Sulawesi, where potato fields cultivated with Granola and its selection, ‘Super John’, were sampled for Potato virus Y (PVY) presence. PVY was found in Sulawesi for the first time. Samples determined to be positive for PVY were subsequently typed to strain using reverse-transcription polymerase chain reaction assays. All PVY isolates sampled were identified as PVYNTN recombinants, with three recombination junctions in P3, VPg, and CP regions of the genome. Three local PVY isolates were subjected to whole-genome sequencing and subsequent sequence analysis. The whole genomes of the Indonesian PVYNTN isolates I-6, I-16, and I-17 were found to be closely related to the European PVYNTN-A. This recombinant type was shown previously to cause potato tuber necrotic ringspot disease (PTNRD) in susceptible potato cultivars. The dependence of potato farmers on mostly a single cultivar, Granola, may have given a competitive advantage to PVYNTN over other PVY strains, resulting in the predominance of the PVYNTN recombinant. The dominance of PVYNTN in Sulawesi, and possibly in Indonesia as a whole, represents a potential risk to any newly introduced potato cultivar to the country, especially cultivars susceptible to PTNRD.

scholarly journals The Role of Aphid Abundance, Species Diversity, and Virus Titer in the Spread of Sweetpotato Potyviruses in Louisiana and Mississippi

Plant Disease ◽  
2013 ◽  
Vol 97 (1) ◽  
pp. 53-61 ◽  
Author(s):  
E. N. Wosula ◽  
J. A. Davis ◽  
C. A. Clark ◽  
T. P. Smith ◽  
R. A. Arancibia ◽  
...  
Keyword(s):  
Species Diversity ◽  
Virus Infection ◽  
Sweet Potato ◽  
Potato Virus ◽  
Virus Titer ◽  
The United States ◽  
Aphid Species ◽  
Sentinel Plants ◽  
Sweet Potato Virus ◽  
Aphid Abundance

Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG), and Sweet potato virus 2 (SPV2) are sweetpotato (Ipomoea batatas) potyviruses nonpersistently transmitted by aphids. Our objective was to determine how aphid abundance, aphid species diversity, and virus titers relate to the spread of SPFMV, SPVG, and SPV2 in Louisiana and Mississippi sweetpotato fields. The most abundant aphid species were Aphis gossypii, Myzus persicae, Rhopalosiphum padi, and Therioaphis trifolii. Aphids were captured during the entire crop cycle but virus infection of sentinel plants occurred mainly during the months of June to August. SPFMV was more commonly detected than SPVG or SPV2 in sentinel plants. Virus titers for SPFMV were higher in samples beginning in late June. Because significant aphid populations were present during April to June when virus titers were low in sweetpotato and there was very little virus infection of sentinel plants, low virus titers may have limited aphid acquisition and transmission opportunities. This is the first study to comprehensively examine aphid transmission of potyviruses in sweetpotato crops in the United States and includes the first report of R. maidis and R. padi as vectors of SPFMV, though they were less efficient than A. gossypii or M. persicae.

scholarly journals Identification of Sweet potato virus G isolated from sweet potato in Japan

2009 ◽  
Vol 75 (2) ◽  
pp. 102-108 ◽  
Author(s):  
S. YAMASAKI ◽  
J. SAKAI ◽  
S. KAMISOYAMA ◽  
K. HANADA
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Sweet Potato Virus

scholarly journals Evaluation of Selected Zambian Popular Sweet Potato Genotypes for Response to Sweet Potato Virus Disease

2020 ◽  
pp. 44-51
Author(s):  
Joseph Banda ◽  
Patrick Chiza Chikoti ◽  
Langa Tembo
Keyword(s):  
Sweet Potato ◽  
Potato Virus ◽  
Virus Disease ◽  
Beta Carotene ◽  
Yield Reduction ◽  
Tuber Weight ◽  
Carotene Content ◽  
Potato Virus Disease ◽  
Sweet Potato Virus ◽  
Sweet Potato Virus Disease

Aim: The objective of this study was to determine the effect of sweet potato virus disease (SPVD) on the beta carotene content, tuber weight and vine weight of selected popular sweet potato genotypes. Study Design: The experiment was laid as a randomized complete block design (RCBD) with three replications. Place and Duration of Study: The experiment was conducted for two cropping seasons (2015/16 and 2016/17) at the Zambia Agriculture Research Institute in Chilanga district of Zambia. Methodology: The uninfected (control) genotypes of Kanga, Chiwoko and Chingovwa were evaluated alongside their SPVD infected genotypes. Genotypic infection was confirmed using molecular approaches, and data was collected at harvest on beta carotene content, tuber weight and vine weight. Results: The results showed that SPVD affects the yield and beta carotene content of sweet potato. Significant differences (P< .001) for yield performance and beta carotene were observed. The yield reduction in percentage across seasons for all genotypes between the uninfected and infected genotypes ranged from 77% to 79% and 67% to 76% for tuber weight and vine weight respectively. Only Chiwoko exhibited higher levels of beta carotene among the genotypes. However, the SPVD infected Chiwoko genotype compared to the uninfected treatment produced mean beta carotene content of 39.1 µg/g and 91.5 µg/g respectively. Conclusion: SPVD reduces the tuber weight, vine weight and beta carotene content in infected sweet potato genotypes.

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