Species and genetic variability of sweet potato viruses in China

AbstractChina is the world’s largest producer of sweet potato (Ipomoea batatas (L.) Lam.). Considering that there are numerous sweet potato-producing regions in China and sweet potato is a vegetatively propagated crop, the genetic diversity of sweet potato viruses could be high in the country. However, studies on species and genetic variabilities of sweet potato viruses in China are limited, making it difficult to prevent and control viral diseases in this crop. During 2014–2019, sweet potato samples with viral disease-like symptoms were randomly collected from sweet potato fields in 25 provinces in China. Twenty-one virus species, including 12 DNA and 9 RNA viruses, were identified in the samples using next-generation sequencing, polymerase chain reaction and rolling-circle amplification methods. One novel sweepovirus species, Sweet potato leaf curl Hubei virus (SPLCHbV), was identified. Two species, Sweet potato collusive virus and Tobacco mosaic virus, were identified for the first time in sweet potato in China. Full-length or nearly full-length genomic sequences of 111 isolates belonging to 18 viral species were obtained. Genome sequence comparisons of potyvirus isolates obtained in this study indicate that the genome of sweet potato virus 2 is highly conserved, whereas the other four potyviruses, sweet potato feathery mottle virus, sweet potato virus G, sweet potato latent virus and sweet potato virus C, exhibited a high genetic variability. The similarities among the 40 sweepovirus genomic sequences obtained from eight sweepovirus species are 67.0–99.8%. The eight sweepoviruses include 14 strains, of which 4 novel strains were identified from SPLCHbV and 1 from sweet potato leaf curl Guangxi virus. Five sweet potato chlorotic stunt virus (SPCSV) isolates obtained belong to the WA strain, and the genome sequences of SPCSV are highly conserved. Together, this study for the first time comprehensively reports the variability of sweet potato viruses in China.

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Occurrence and genetic variability of partial coat protein gene of Sweet potato leaf curl virus (SPLCV) in Kenya

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2017.15969 ◽

2017 ◽

Vol 16 (45) ◽

pp. 2112-2120 ◽

Cited By ~ 2

Author(s):

S. Maina ◽

D. W. Miano ◽

E. Mbogo ◽

J. O. Amimo ◽

J. Irungu ◽

...

Keyword(s):

Coat Protein ◽

Genetic Variability ◽

Sweet Potato ◽

Coat Protein Gene ◽

Protein Gene ◽

Potato Leaf ◽

Leaf Curl Virus ◽

Leaf Curl

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Infeksi Alami Pepper Yellow Leaf Curl Virus dan Sweet potato virus C Pada Ubi Jalar di Malang, Jawa Timur

Jurnal Fitopatologi Indonesia ◽

10.14692/jfi.15.6.248-254 ◽

2019 ◽

Vol 15 (6) ◽

pp. 248-254

Author(s):

Tri Asmira Damayanti ◽

Anastasya Hondo ◽

Yusmani Prayogo

Keyword(s):

Sweet Potato ◽

Potato Virus ◽

Yellow Leaf ◽

Sweet Potato Virus ◽

Virus C ◽

Leaf Curl Virus ◽

Leaf Curl

Gejala tulang daun kuning (vein yellowing) dan malformasi daun yang diduga disebabkan oleh virus ditemukan pada ubi jalar IR Melati di daerah Kendalpayak, Malang, Jawa Timur. Amplifikasi DNA/cDNA menggunakan primer universal Begomovirus, Potyvirus, dan Cucumovirus menunjukkan positif teramplifikasi DNA dengan primer universal Begomovirus, dan Potyvirus, namun negatif dengan primer universal Cucumovirus. Berdasarkan runutan sikuen nukleotida, gejala tulang daun kuning dan malformasi daun disebabkan oleh infeksi ganda Pepper yellow leaf curl virus (PYLCV) dan Sweet potato virus C (SPVC). Analisis identitas DNA dengan perangkat lunak BioEdit menunjukkan homologi paling tinggi sebesar 98.5% terhadap PYLCV isolat cabai dari Bangli Bali, dan sebesar 98% terhadap SPVC dengan isolat ubi jalar asal Jepang dan Amerika Serikat. Laporan ini merupakan temuan baru infeksi alami PYLCV dan SPVC pada ubi jalar di Indonesia.

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First Report of Sweet potato golden vein associated virus Infecting Sweet Potato in Korea

Plant Disease ◽

10.1094/pdis-02-14-0123-pdn ◽

2014 ◽

Vol 98 (8) ◽

pp. 1163-1163

Author(s):

E.-J. Kil ◽

J. Kim ◽

H.-S. Byun ◽

H.-R. Kwak ◽

M.-K. Kim ◽

...

Keyword(s):

Sweet Potato ◽

Potato Virus ◽

Leaf Roll ◽

The United States ◽

Viral Gene ◽

Sweet Potatoes ◽

Potato Leaf ◽

Viral Dna ◽

First Report ◽

Sweet Potato Virus

Sweet potato (Ipomoea batatas) is one of the most important crops in eastern Asia, including Korea. Consumption of sweet potato is increasing gradually because of its growing reputation as a health food. Recently, outbreaks of viruses infecting sweet potatoes have increased all over the world, probably because sweet potatoes are produced via vegetative propagation (1,2). In Korea, most sweet potatoes in fields have been infected by a begomovirus, Sweet potato leaf curl virus (SPLCV), and other viruses such as Sweet potato feathery mottle virus, Sweet potato virus G, and Sweet potato latent virus (3). Many countries have monitored sweet potato virus infections in fields as well as in germplasm collections to select virus-free stocks. In 2013, 20 sweet potato plants showing leaf roll symptoms in Muan, South Korea, were collected and analyzed. Total DNA was isolated from sweet potato leaves (Viral Gene-spin Viral DNA/RNA Extraction Kit, iNtRON Biotechnology, Seongnam, Korea) and viral DNA was amplified by rolling circle amplification (RCA, TempliPhi Amplification Kit, GE Healthcare Life Sciences, Uppsala, Sweden) following the manufacturer's instructions. Amplicons were digested by restriction enzyme SacI (TaKaRa Bio, Shiga, Japan) and products were run on a 1.5% agarose gel. A 2.8-kb DNA fragment was purified from a gel, ligated into a pGEM-T easy vector (Promega, Madison, WI), and sequenced (Macrogen, Seoul, Korea). Based on a BLAST search, most of the sequences (36/38) were identified as SPLCV, but two independent clones 2,824 nt in length from sweet potato cv. Sincheonmi were similar to Sweet potato golden vein associated virus (SPGVaV) isolate US:MS:1B-3 (94.38%, GenBank Accession No. HQ333143). The complete genome sequence of the SPGVaV-Korea isolate contained six ORFs, as expected for a typical monopartite begomovirus. The sequence was deposited in GenBank under accession number KF803170. SPGVaV is a whitefly (Bemisia tabaci)-transmitted virus (genus Begomovirus, family Geminiviridae). A phylogenetic analysis that included other begomoviruses that infect sweet potato showed SPGVaV-Korea to segregate with other SPGVaV isolates. SPGVaV has previously only been reported in Brazil and the United States (1). This is the first report of SPGVaV in sweet potato outside of the Americas. References: (1) L. C. Albuquerque et al. Virol. J. 9:241, 2012. (2) E. Choi et al. Acta Virol. 56:187, 2012. (3) H. R. Kwak et al. Plant Pathol. J. 22:239, 2006.

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Potyvirus Complexes in Sweetpotato: Occurrence in Australia, Serological and Molecular Resolution, and Analysis of the Sweet potato virus 2 (SPV2) Component

Plant Disease ◽

10.1094/pd-90-1120 ◽

2006 ◽

Vol 90 (9) ◽

pp. 1120-1128 ◽

Cited By ~ 33

Author(s):

Fred Tairo ◽

Roger A. C. Jones ◽

Jari P. T. Valkonen

Keyword(s):

Sweet Potato ◽

Potato Virus ◽

Restriction Analysis ◽

Degenerate Primers ◽

Protein Encoding ◽

Tentative Member ◽

Sweet Potato Virus ◽

Polymerase Chain ◽

Primer Polymerase Chain Reaction ◽

First Time

A survey for viruses in sweetpotato revealed the presence of Sweet potato virus 2 (SPV2; synonymous to Sweet potato virus Y and Ipomoea vein mosaic virus), a tentative member of the genus Potyvirus, for the first time in Australia. The SPV2-infected sweetpotato plants were also infected with strains RC and/or C of Sweet potato feathery mottle virus (SPFMV; genus Potyvirus). Five SPV2 and SPFMV isolates from Australia were sequence-characterized at the 3′ - proximal end (ca. 1.8 kb) of the genome. A simple and sensitive diagnostic procedure was devised to readily differentiate SPV2 and the two strains of SPFMV from sweetpotato plants that contained these viruses in complexes. The method involved reverse transcription with oligoT25 primer, polymerase chain reaction using a combination of degenerate primers, and restriction analysis of the 1.8-kb amplification products with HindIII and PvuII endonucleases. The Nproximal 543 nucleotides of the SPV2 coat protein-encoding sequence of the Australian isolates and 14 other isolates from Asia, Africa, Europe, and North America were subjected to phylogenetic analysis. The Australian SPV2 isolates formed a separate clade that was closest to a clade containing two North American isolates.

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Diversity of Sweepoviruses Infecting Sweet Potato in China

Plant Disease ◽

10.1094/pdis-04-17-0524-re ◽

2017 ◽

Vol 101 (12) ◽

pp. 2098-2103 ◽

Cited By ~ 4

Author(s):

Qili Liu ◽

Yongjiang Wang ◽

Zhenchen Zhang ◽

Hui Lv ◽

Qi Qiao ◽

...

Keyword(s):

Sweet Potato ◽

Open Reading Frames ◽

Genomic Sequences ◽

Potato Leaf ◽

The Family ◽

Different Strains ◽

Leaf Curl Virus ◽

Reading Frames ◽

Recombination Breakpoints ◽

Leaf Curl

Sweepoviruses (a group of begomoviruses that infect plants in the family Convolvulaceae) have monopartite genomes that consist of a circular, single-stranded DNA molecule. Seventy-three complete genomic sequences of sweepoviruses were characterized from the sweet potato samples collected in China. Eight sweepovirus species, including two novel species with proposed names of Sweet potato leaf curl China virus 2 and Sweet potato leaf curl Sichuan virus 2, were identified among these samples. One species, Sweet potato leaf curl Canary virus, was first identified in China. Among the 13 identified strains of Chinese sweepoviruses, 4 were newly discovered. Sweet potato leaf curl virus had the highest frequency (53.4%) of occurrence in the sweet potato samples from China. The similarities among the 73 sweepovirus genomic sequences were between 77.6 and 100.0%. Multiple recombination events were identified, and 16 recombinant sequences were determined. Recombination was observed between different species and between different strains of the same species. Recombination breakpoints were mainly localized on the intergenic region and in three open reading frames (AC1, AV1, and AV2). This study is the first comprehensive report on the genetic diversity of sweepoviruses in China.

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Large-Scale Seedling Grow-Out Experiments Do Not Support Seed Transmission of Sweet Potato Leaf Curl Virus in Sweet Potato

Plants ◽

10.3390/plants10010139 ◽

2021 ◽

Vol 10 (1) ◽

pp. 139

Author(s):

Sharon A. Andreason ◽

Omotola G. Olaniyi ◽

Andrea C. Gilliard ◽

Phillip A. Wadl ◽

Livy H. Williams ◽

...

Keyword(s):

Sweet Potato ◽

Large Scale ◽

Seed Transmission ◽

Potato Production ◽

Potato Leaf ◽

Vector Transmission ◽

Leaf Curl Virus ◽

Seed Coats ◽

Potato Seedlings ◽

Leaf Curl

Sweet potato leaf curl virus (SPLCV) threatens global sweet potato production. SPLCV is transmitted by Bemisia tabaci or via infected vegetative planting materials; however, SPLCV was suggested to be seed transmissible, which is a characteristic that is disputed for geminiviruses. The objective of this study was to revisit the validity of seed transmission of SPLCV in sweet potato. Using large-scale grow-out of sweet potato seedlings from SPLCV-contaminated seeds over 4 consecutive years, approximately 23,034 sweet potato seedlings of 118 genotype entries were evaluated. All seedlings germinating in a greenhouse under insect-proof conditions or in a growth chamber were free of SPLCV; however, a few seedlings grown in an open bench greenhouse lacking insect exclusion tested positive for SPLCV. Inspection of these seedlings revealed that B. tabaci had infiltrated the greenhouse. Therefore, transmission experiments were conducted using B. tabaci MEAM1, demonstrating successful vector transmission of SPLCV to sweet potato. Additionally, tests on contaminated seed coats and germinating cotyledons demonstrated that SPLCV contaminated a high percentage of seed coats collected from infected maternal plants, but SPLCV was never detected in emerging cotyledons. Based on the results of grow-out experiments, seed coat and cotyledon tests, and vector transmission experiments, we conclude that SPLCV is not seed transmitted in sweet potato.

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Control strategies for sweet potato virus disease in Africa

Virus Research ◽

10.1016/j.virusres.2003.12.023 ◽

2004 ◽

Vol 100 (1) ◽

pp. 115-122 ◽

Cited By ~ 29

Author(s):

Richard W. Gibson ◽

Valentine Aritua ◽

Emmanuel Byamukama ◽

Isaac Mpembe ◽

James Kayongo

Keyword(s):

Sweet Potato ◽

Potato Virus ◽

Virus Disease ◽

Control Strategies ◽

Potato Virus Disease ◽

Sweet Potato Virus ◽

Sweet Potato Virus Disease

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C4 Protein of Sweet Potato Leaf Curl Virus Regulates Brassinosteroid Signaling Pathway through Interaction with AtBIN2 and Affects Male Fertility in Arabidopsis

Frontiers in Plant Science ◽

10.3389/fpls.2017.01689 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 14

Author(s):

Huiping Bi ◽

Weijuan Fan ◽

Peng Zhang

Keyword(s):

Signaling Pathway ◽

Sweet Potato ◽

Male Fertility ◽

Potato Leaf ◽

Brassinosteroid Signaling ◽

Leaf Curl Virus ◽

Leaf Curl

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An Improved Multiplex IC-RT-PCR Assay Distinguishes Nine Strains of Potato virus Y

Plant Disease ◽

10.1094/pdis-02-13-0161-sr ◽

2013 ◽

Vol 97 (10) ◽

pp. 1370-1374 ◽

Cited By ~ 31

Author(s):

Mohamad Chikh-Ali ◽

Stewart M. Gray ◽

Alexander V. Karasev

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Rna Extraction ◽

Leaf Tissue ◽

Rt Pcr ◽

Pcr Assay ◽

Potato Leaf ◽

Extraction Step ◽

First Time ◽

Virus Testing

A multiplex reverse-transcription polymerase chain reaction (RT-PCR) assay was previously developed to identify a group of Potato virus Y (PVY) isolates with unusual recombinant structures (e.g., PVYNTN-NW and SYR-III) and to differentiate them from other PVY strains. In the present study, the efficiency of this multiplex RT-PCR assay was validated and extended considerably to include five additional strains and strain groups not tested before. To make the multiplex RT-PCR assay more applicable and suitable for routine virus testing and typing, it was modified by replacing the conventional RNA extraction step with the immunocapture (IC) procedure. The results obtained using well-characterized reference isolates revealed, for the first time, that this multiplex RT-PCR assay is an accurate and robust method to identify and differentiate the nine PVY strains reported to date, including PVYO (both PVYO and PVYO-O5), PVYN, PVYNA-N, PVYNTN, PVYZ, PVYE, PVY-NE11, PVYN-Wi, and PVYN:O, which is not possible by any of the previously reported RT-PCR procedures. This would make the IC-RT-PCR procedure presented here a method of choice to identify PVY strains and assess the strain composition of PVY in a given area. The IC-RT-PCR protocol was successfully applied to typing PVY isolates in potato leaf tissue collected in the field.

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