Extreme resistance to potato virus A in potato cultivar Barbara is independently mediated by Ra and Rysto

Potato virus A (PVA) and potato virus Y (PVY) are two common members of Potyvirus genus infecting potato crops worldwide. Host resistance offers an economical and effective means for the control and/or management of these viruses. In this study, 20 potato clones were screened for their resistance against PVA and PVY by mechanical and/or graft inoculation assay, and were explored for the relationship between extreme resistance genes Ra and Ry by the detection of molecular markers linked respectively to Ryadg, Rysto, and Rychc. Six clones, including Barbara, Jizhangshu 8, Longshu 7, Longshu 8, M6, and Solara, were found to be extremely resistant to both PVA and PVY; three clones (AC142, Eshu 3, and Shepody) were deemed to be extremely resistant to PVA but susceptible to PVY. To further reveal the inheritance of the extreme resistance (ER) against PVA, a tetraploid F1 population of Barbara × F58050 (susceptible to both PVY and PVA) and a tetraploid BC1 population of BF145 (a PVA-resistant but PVY-susceptible progeny of Barbara × F58050) × F58050 were obtained, and phenotyping of the F1 and BC1 population by graft-inoculation with PVA showed segregation ratios of 3:1 and 1:1 (R:S), respectively. These results suggested that two independent loci control ER against PVA in Barbara: one confers ER to both PVA and PVY, and the other confers ER to PVA only. The deduced genotype of Barbara is RyryryryRararara.

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Resistance to potato virus A and potato virus Y in potato cultivars grown in Finland

Agricultural and Food Science ◽

10.23986/afsci.72730 ◽

1996 ◽

Vol 5 (1) ◽

pp. 57-62 ◽

Cited By ~ 4

Author(s):

Jari P. T. Valkonen ◽

Jukka P. Palohuhta

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Phenotypic Expression ◽

Leaf Tissue ◽

Systemic Infection ◽

Potato Cultivars ◽

Extreme Resistance ◽

Hypersensitive Resistance ◽

Potato Virus A ◽

Das Elisa

Phenotypic expression of resistance to potato Y potyvirus (PVY) and potato A potyvirus (PVA) was tested in 24 potato cultivars and an advanced breeding line using graft-inoculation under controlled conditions in the glasshouse. Resistance phenotypes were determined based on symptom expression and systemic infection detected with DAS-ELISA. Tubers were harvested from the PVA-inoculated plants and tested for PVA with ELISA. Sixteen potato cultivars expressed hypersensitive resistance (HR) to the strain group YO of PVY. Ute expressed extreme resistance (ER) to PVY (strain groups YO and YN) and PVA, and eight cultivars (Amazone, Bintje, Fambo, Posmo, Record, Rosamunda, Saturna and Van Gogh) expressed ER to PVA. These cultivars produced no PVA-infected tubers (tubers of Record were not tested). Matilda and Nicola expressed HR to PVA. The tubers of graft-inoculated Matilda produced no PVA-infected shoots, whereas shoots from Nicola tubers developed necrosis and severe mosaic symptoms and were PVA-infected based on results from ELISA. Comparison with purified PVA antigen (using ELISA) indicated that the secondarily infected shoots of the 14 PVA-susceptible cultivars contained 206-804 ng of PVA antigen per gram of leaf tissue.

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Mutations in Potato virus Y Genome-Linked Protein Determine Virulence Toward Recessive Resistances in Capsicum annuum and Lycopersicon hirsutum

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2004.17.3.322 ◽

2004 ◽

Vol 17 (3) ◽

pp. 322-329 ◽

Cited By ~ 145

Author(s):

Benoît Moury ◽

Caroline Morel ◽

Elisabeth Johansen ◽

Laurent Guilbaud ◽

Sylvie Souche ◽

...

Keyword(s):

Amino Acid ◽

Capsicum Annuum ◽

Potato Virus ◽

Potato Virus Y ◽

Cdna Clones ◽

Lycopersicon Hirsutum ◽

Potato Virus A ◽

Single Nucleotide Change ◽

Protein Encoding

The recessive resistance genes pot-1 and pvr2 in Lycopersicon hirsutum and Capsicum annuum, respectively, control Potato virus Y (PVY) accumulation in the inoculated leaves. Infectious cDNA molecules from two PVY isolates differing in their virulence toward these resistances were obtained using two different strategies. Chimeras constructed with these cDNA clones showed that a single nucleotide change corresponding to an amino acid substitution (Arg119His) in the central part of the viral protein genome-linked (VPg) was involved in virulence toward the pot-1 resistance. On the other hand, 15 nucleotide changes corresponding to five putative amino acid differences in the same region of the VPg affected virulence toward the pvr21 and pvr22 resistances. Substitution models identified six and five codons within the central and C terminal parts of the VPg for PVY and for the related potyvirus Potato virus A, respectively, which undergo positive selection. This suggests that the role of the VPg-encoding region is determined by the protein and not by the viral RNA apart from its protein-encoding capacity.

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Inheritance of extreme resistance to potato virus Y in potato

Potato Research ◽

10.1007/bf02357933 ◽

1995 ◽

Vol 38 (2) ◽

pp. 199-210 ◽

Cited By ~ 4

Author(s):

Bogdan Flis

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Extreme Resistance

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Occurrence and Molecular Characterization of Recombinant Potato virus YNTN Isolates from Sulawesi, Indonesia

Plant Disease ◽

10.1094/pdis-07-15-0817-re ◽

2016 ◽

Vol 100 (2) ◽

pp. 269-275 ◽

Cited By ~ 11

Author(s):

Mohamad Chikh-Ali ◽

Nilsa A. Bosque-Pérez ◽

Dalton Vander Pol ◽

Dantje Sembel ◽

Alexander V. Karasev

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Potato Cultivar ◽

Whole Genome ◽

Chain Reaction ◽

Recombinant Type ◽

Whole Genomes ◽

Polymerase Chain ◽

First Time

The importance of potato has increased dramatically in Indonesia over the last three decades. During this period, ‘Granola’, a potato cultivar originally from Germany, has become the most common cultivar for fresh consumption in Indonesia. In August 2014, a survey was conducted in Sulawesi, where potato fields cultivated with Granola and its selection, ‘Super John’, were sampled for Potato virus Y (PVY) presence. PVY was found in Sulawesi for the first time. Samples determined to be positive for PVY were subsequently typed to strain using reverse-transcription polymerase chain reaction assays. All PVY isolates sampled were identified as PVYNTN recombinants, with three recombination junctions in P3, VPg, and CP regions of the genome. Three local PVY isolates were subjected to whole-genome sequencing and subsequent sequence analysis. The whole genomes of the Indonesian PVYNTN isolates I-6, I-16, and I-17 were found to be closely related to the European PVYNTN-A. This recombinant type was shown previously to cause potato tuber necrotic ringspot disease (PTNRD) in susceptible potato cultivars. The dependence of potato farmers on mostly a single cultivar, Granola, may have given a competitive advantage to PVYNTN over other PVY strains, resulting in the predominance of the PVYNTN recombinant. The dominance of PVYNTN in Sulawesi, and possibly in Indonesia as a whole, represents a potential risk to any newly introduced potato cultivar to the country, especially cultivars susceptible to PTNRD.

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