scholarly journals First Report of Meloidogyne marylandi Infecting Bermudagrass in Florida

Plant Disease ◽  
2012 ◽  
Vol 96 (10) ◽  
pp. 1583-1583 ◽  
Author(s):  
N. S. Sekora ◽  
W. T. Crow ◽  
T. Mekete

Root-knot nematodes (Meloidogyne spp.) are common parasites attacking turfgrasses in the United States, but the species of these nematodes is typically unresolved unless targeted surveys are performed (3). Using morphometric analysis and an RFLP method (3), an investigation of a golf course green in Florida with a history of infestation by root-knot nematodes was conducted to identify the species present. This ‘Tifdwarf’ bermudagrass (Cynodon dactylon × C. transvaalensis) putting green at the University of Florida Research Unit in Citra, FL, exhibited irregular patches of declining turf. Turf roots in these symptomatic areas had galled root tips with adhering egg masses, characteristic of infection from Meloidogyne spp. Mean populations of 5,149 ± 708 Meloidogyne second stage juveniles per 100 cm3 of soil were extracted from the rhizosphere of these symptomatic plants. Morphological measurements from 20 of these juveniles were slightly less than those published previously for M. marylandi, but were still distinct enough to discriminate them from M. graminis, which commonly infects bermudagrass in Florida (3). Body length averaged 396.1 ± 4.9 (376.7 to 420.0) μm with a mean width of 16.3 ± 0.5 (13.3 to 18.3) μm, stylet lengths were 11.2 ± 0.7 (6.7 to 12.3) μm, tail lengths averaged 54.7 ± 1.9 (47.5 to 65.0) μm with the hyaline region of the tails 9.9 ± 0.7 (8.3 to 14.2) μm. Mature females extracted from symptomatic root tissue lacked a posterior cone-like protuberance of the vulva typical of M. graminis. DNA was extracted from 15 single juveniles using a NaOH digestion method (2). The mitochondrial DNA region was amplified with PCR using the primers C2F3/1108 5′-GGTCAATGTTCAGAAATTTGTGG-3′ and 5′-TACCTTTGACCAATCACGCT-3′ (3). This resulted in a DNA fragment 520 bp in length, which upon digestion with SspI restriction enzyme produced four bands 148, 103, 91, and 67 bp in length, similar to what has been reported for M. marylandi (3). The PCR products were purified with a QIAquick PCR purification kit (QIAGEN, Valencia, CA) and sequenced at the University of Florida, Cancer Research and Genetics Institute. Sequences were compared with those in GenBank by means of BLAST search. The comparison showed a sequence similarity of 98% with M. marylandi (GenBank Accession No. JN241918.1). Although M. marylandi has been reported on bermudagrass in many areas of the United States and other places throughout the world (1,3,4), to our knowledge, this is the first detection of this nematode in Florida. Further studies will be conducted to determine the prevalence, incidence, severity of damage caused by M. marylandi, and determine a possible mode of dispersal on turfgrasses. References: (1) A. M. Golden. J. Nematol. 21:453, 1989. (2) J. Hübschen et al. Euro. J. Plant Pathol. 110:779, 2004. (3) M. A. McClure et al. Plant Dis. 96:635, 2012. (4) Y. Oka et al. Nematol. 5:727, 2003.

2009 ◽  
Vol 71 (3) ◽  
pp. 206-209 ◽  
Author(s):  
Jeffrey E. Hill ◽  
Kathy Heym Kilgore ◽  
Deborah B. Pouder ◽  
James F. F. Powell ◽  
Craig A. Watson ◽  
...  

Author(s):  
Kira Nagoshi ◽  
Zareen Zaidi ◽  
Ashleigh Wright ◽  
Carolyn Stalvey

Purpose: Peer-assisted learning (PAL) promotes the development of communication, facilitates improvements in clinical skills, and is a way to provide feedback to learners. We utilized PAL as a conceptual framework to explore the feasibility of peer-assisted feedback (PAF) to improve note-writing skills without requiring faculty time. The aim was to assess whether PAL was a successful method to provide feedback on the United States Medical Licensing Exams (USMLE)-style clinical skills exam notes by using student feedback on a survey in the United States.Methods: The University of Florida College of Medicine administers clinical skills examination (CSEs) that include USMLE-like note-writing. PAL, in which students support the learning of their peers, was utilized as an alternative to faculty feedback. Second-year (MS2) and third-year (MS3) medical students taking CSEs participated in faculty-run note-grading sessions immediately after testing, which included explanations of grading rubrics and the feedback process. Students graded an anonymized peer’s notes. The graded material was then forwarded anonymously to its student author to review. Students were surveyed on their perceived ability to provide feedback and the benefits derived from PAF using a Likert scale (1–6) and open-ended comments during the 2017–2018 academic year.Results: Students felt generally positively about the activity, with mean scores for items related to educational value of 4.49 for MS2s and 5.11 for MS3s (out of 6). MS3s perceived peer feedback as constructive, felt that evaluating each other’s notes was beneficial, and felt that the exercise would improve their future notes. While still positive, MS2 students gave lower scores than the MS3 students.Conclusion: PAF was a successful method of providing feedback on student CSE notes, especially for MS3s. MS2s commented that although they learned during the process, they might be more invested in improving their note-writing as they approach their own USMLE exam.


Plant Disease ◽  
1998 ◽  
Vol 82 (10) ◽  
pp. 1172-1172 ◽  
Author(s):  
T. A. Kucharek ◽  
D. E. Purcifull ◽  
R. G. Christie ◽  
K. D. Perkins

Since 1995, severe epidemics of cucumber mosaic virus (CMV) have occurred in select fields of tobacco (Nicotiana tabacum) and pepper (Capsicum annuum) in three counties in northern Florida. Yield losses greater than 50% have occurred in both crops. Baker and Zettler (1) identified the presence of CMV in one plant of tropical spiderwort (Commelina benghalensis) in an organic garden on the campus of the University of Florida 10 years ago. In addition, they infected tropical spiderwort and Asiatic dayflower (Commelina communis) with isolates of CMV. Since 1995, in one area of northern Alachua County, Asiatic dayflower has been found in abundance in and around some fields and found to be infected with CMV. Prior to this time, CMV had not been known to be epidemic in any crop in northern Florida. Also, commelinaceous weeds did not occur in such abundance in northern Florida. In Hamilton County, an epidemic of CMV occurred in one field of tobacco in 1997. Tropical spiderwort with viral-like symptoms was growing abundantly in that field. The symptoms in this weed included chlorotic ringspots and chevron-like line patterns. Light microscopy, with Azure A stain, revealed the presence of typical inclusions of CMV in pepper, tobacco, tropical spiderwort, and Asiatic dayflower. Symptomatic samples of the tobacco and the tropical spiderwort reacted in an immunodiffusion test with antiserum to a winged bean isolate of CMV (2). Extracts from tropical spiderwort (isolate 3603) were rubbed on squash. This isolate was thereafter maintained in squash (Cucurbita pepo cvs. Prelude II or Early Prolific Straightneck). Infected plants of both of these cultivars developed strong mosaic symptoms and were stunted. After passage through squash, the 3603 isolate induced mosaic in tobacco (cv. Burley 21). Some plants of the squash cultivars Destiny III and Liberator III, which have transgenic, coat protein-mediated resistance to CMV, developed restricted symptoms after inoculation with this isolate. CMV was recovered by back inoculation from symptomatic plants of these cultivars. Symptomless plants of tropical spiderwort transplanted from the field developed chlorotic ringspots and chevron-like line patterns following inoculation in the greenhouse with isolate 3603. Back inoculations to squash followed by immunodiffusion assays confirmed the presence of CMV in the inoculated tropical spiderwort plants but CMV was not detected in noninoculated control plants. This is the first report of tropical spiderwort being infected with CMV in a commercial situation in the United States. Because commelinaceous plants are well known to be excellent hosts of CMV (1), we believe that the increased presence of perennial, commelinaceous weeds is a factor contributing to the epidemics of CMV in northern Florida. References: (1) C. A. Baker and F. W. Zettler. Plant Dis. 72:513, 1988. (2) C. A. Ku-wite and D. E. Purcifull. Plant Dis. 66:1071, 1982.


1996 ◽  
Vol 1 (1) ◽  
pp. 3-24 ◽  
Author(s):  
Alan Rodger

This article is the revised text of the first W A Wilson Memorial Lecture, given in the Playfair Library, Old College, in the University of Edinburgh, on 17 May 1995. It considers various visions of Scots law as a whole, arguing that it is now a system based as much upon case law and precedent as upon principle, and that its departure from the Civilian tradition in the nineteenth century was part of a general European trend. An additional factor shaping the attitudes of Scots lawyers from the later nineteenth century on was a tendency to see themselves as part of a larger Englishspeaking family of lawyers within the British Empire and the United States of America.


2015 ◽  
Vol 36-37 (1) ◽  
pp. 163-183
Author(s):  
Paul Taylor

John Rae, a Scottish antiquarian collector and spirit merchant, played a highly prominent role in the local natural history societies and exhibitions of nineteenth-century Aberdeen. While he modestly described his collection of archaeological lithics and other artefacts, principally drawn from Aberdeenshire but including some items from as far afield as the United States, as a mere ‘routh o’ auld nick-nackets' (abundance of old knick-knacks), a contemporary singled it out as ‘the best known in private hands' (Daily Free Press 4/5/91). After Rae's death, Glasgow Museums, National Museums Scotland, the University of Aberdeen Museum and the Pitt Rivers Museum in Oxford, as well as numerous individual private collectors, purchased items from the collection. Making use of historical and archive materials to explore the individual biography of Rae and his collection, this article examines how Rae's collecting and other antiquarian activities represent and mirror wider developments in both the ‘amateur’ antiquarianism carried out by Rae and his fellow collectors for reasons of self-improvement and moral education, and the ‘professional’ antiquarianism of the museums which purchased his artefacts. Considered in its wider nineteenth-century context, this is a representative case study of the early development of archaeology in the wider intellectual, scientific and social context of the era.


2019 ◽  
Author(s):  
Ani Eblighatian

The paper is an off-shoot of the author's PhD project on lamps from Roman Syria (at the University of Geneva in Switzerland), centered mainly on the collection preserved at the Art Museum of Princeton University in the United States. One of the outcomes of the research is a review of parallels from archaeological sites and museum collections and despite the incomplete documentation i most cases, much new insight could be gleaned, for the author's doctoral research and for other issues related to lychnological studies. The present paper collects the data on oil lamps from byzantine layers excavated in 1932–1939 at Antioch-on-the-Orontes and at sites in its vicinity (published only in part so far) and considers the finds in their archaeological context.


1993 ◽  
Vol 67 (1) ◽  
pp. 151-151
Author(s):  
R. William Orr ◽  
Richard H. Fluegeman

In 1990 (Fluegeman and Orr) the writers published a short study on known North American cyclocystoids. This enigmatic group is best represented in the United States Devonian by only two specimens, both illustrated in the 1990 report. Previously, the Cortland, New York, specimen initially described by Heaslip (1969) was housed at State University College at Cortland, New York, and the Logansport, Indiana, specimen was housed at Ball State University, Muncie, Indiana. Both institutions recognize the importance of permanently placing these rare specimens in a proper paleontologic repository with other cyclocystoids. Therefore, these two specimens have been transferred to the curated paleontologic collection at the University of Cincinnati Geological Museum where they can be readily studied by future workers in association with a good assemblage of Ordovician specimens of the Cyclocystoidea.


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