scholarly journals First Report of Clover Yellow Vein Virus in Crotalaria micans in Hawaii

Plant Disease ◽  
2020 ◽  
Vol 104 (12) ◽  
pp. 3276-3276
Author(s):  
J. Dahan ◽  
G. E. Orellana ◽  
X. Feng ◽  
A. T. Kong ◽  
R. T. Hamasaki ◽  
...  
Keyword(s):  
Yellow Vein ◽  
First Report ◽  
Clover Yellow Vein Virus

scholarly journals First report of clover yellow vein virus on orchid (Dendrobium sp.) in South Korea

Plant Disease ◽  
2021 ◽  
Author(s):  
Ju-Yeon Yoon ◽  
In Sook Cho ◽  
Bong Nam Chung ◽  
Seung-Kook Choi
Keyword(s):  
South Korea ◽  
Complete Genome ◽  
High Throughput Sequencing ◽  
Yellow Vein ◽  
Nucleotide Identity ◽  
Virus Species ◽  
Total Rna ◽  
First Report ◽  
Three Samples ◽  
Clover Yellow Vein Virus

Orchid is one of the most popular and commercially important cultivated flowers in the world. Among many orchid species, Dendrobium species are popular cut flowers and potted plants in South Korea. In March 2019, 10 Dendrobium orchid plants in a greenhouse in Daegu, South Korea showed large chlorotic blotches, mosaic and mottle symptoms. One leaf each from the 10 symptomatic orchid plants by leaf dip-preparations and transmission electron microscopy (JEM-1400; JEOL Inc., Tokyo, Japan) after leaf dip-preparations (Brenner and Horne 1959; Richert-Pöggeler et al. 2019). Typical potyvirus-like particles of flexuous and filamentous shape and ∼ 760 × 15 nm length/width were observed in all tested samples. The presence of potyvirus was confirmed by serological detection with a commercially available ImmunoStrip® for potyvirus group (Agdia, Elkhart, USA). In contrast, a negative result was obtained for a virus-free Dendrobium plant by the serological test. The two most common viruses in orchids, namely odontoglossum ringspot virus (ORSV) and cymbidium mosaic virus (CymMV) in all Dendrobium samples were not detected in any samples by an ImmunoStrip® for ORSV and CymMV (Agdia, Elkhart, USA). To determine the species of the virus, total RNA was extracted from all 10 ImmunoStrip®-positive samples using the RNeasy plant mini kit (Qiagen, Hilden, Germany). Subsequently, reverse transcription-PCR (RT-PCR) products (~1,625 bp) were amplified using potyvirus- specific primer pair (Gibbs and Mackenzie, 1997) and sequenced by the Sanger method at Macrogen (Seoul, South Korea). Sequencing results showed 100% nucleotide identity among 10 samples. Thus, one sequence was chosen for identification of virus species using sequence comparison. BLASTn analysis showed that the nucleotide sequence and its deduced amino acid sequence of the amplicon shared 95.4-98.7% and 96.2-99.6% identity to multiple clover yellow vein virus (ClYVV) sequences (e.g., accession no. AB011819) in GenBank. To further confirm the presence of ClYVV and determine if other viral agents were present in the samples, total RNA from three of the 10 symptomatic plants was depleted of ribosomal RNAs and subjected to high-throughput sequencing (HTS) analysis on a HiSeq 4000 platform (Macrogen Inc., Seoul, South Korea). A total of 3,764,432, 4,203,881, and 4,139,775 of 150-bp paired-end clean reads were obtained for the three samples. After de novo assembly of the reads with Trinity (Haas et al. 2013), 5, 6 and 7 contigs were obtained and searched with BLASTn against NCBI viral refseq database. Eighteen contigs from all three samples sized at 2,176-9,432 nt exhibited 94.0-97.9% nucleotide identity with the complete genome sequences of other ClYVV isolates (e.g., accession no. AB011819) deposited in Genbank; no other viruses were identified by HTS. The complete genome sequence (9,585 nucleotides in length) of ClYVV Dendrobium isolate (ClYVV-Den) was determined using ClYVV-specific primers (Takahashi et al., 1997) and the sequence of CIYVV-Den was deposited to GenBank (Accession no. LC506604). Together, these results support that symptomatic Dendrobium orchids were infected with ClYVV-Den in this study. ClYVV has been previously reported affecting Calanthe orchids in Japan (Inouye et al., 1988; Ikegami et al., 1995). Our results suggest that ClYVV may be detrimental to the production of Dendrobium orchids or commercial ornamental crops in South Korea. To our knowledge, this is the first report of ClYVV in Dendrobium sp. in South Korea.

scholarly journals First Report of Clover yellow vein virus in Grain Legumes in Spain

Plant Disease ◽  
2009 ◽  
Vol 93 (1) ◽  
pp. 106-106 ◽  
Author(s):  
V. Ortiz ◽  
S. Castro ◽  
J. Romero
Keyword(s):  
Broad Bean ◽  
Grain Legumes ◽  
Genetic Distances ◽  
Yellow Vein ◽  
Yellow Mosaic Virus ◽  
Economic Losses ◽  
Rt Pcr ◽  
Sequence Alignments ◽  
First Report ◽  
Clover Yellow Vein Virus

From 1999 to 2002, field surveys were conducted in the legume-growing areas of Spain including Ávila, Badajoz, Cádiz, Córdoba, León, Málaga, Murcia, Salamanca, and Zamora provinces. Leaf tissue from 35 asymptomatic and 224 virus symptomatic plants was sampled and analyzed by indirect-ELISA with a specific monoclonal antibody against the potyvirus group (Adgia, Elkhart, IN). All symptomatic plants of bean (Phaseolus vulgaris L.), broad bean (Vicia faba L.), lentils (Lens culinaris L.), and chickpea (Cicer arietinum L.) were positive for potyvirus infection. Identification as Bean yellow mosaic virus (BYMV) was obtained by double-antibody sandwich (DAS)-ELISA with a polyclonal antiserum (Loewe Biochemica Gmbh, Sauerlach, Germany). To analyze the genetic variability of BYMV Spanish isolates, 33 Spanish isolates were selected at random from our BYMV collection, and extracts from these plants were used with primers 1985 (5′-gagagaatgatacacatactgaa-3′) and 1984 (5′-caaggtgagtggacaatgatgg-3′) to amplify by immunocapture (IC)-reverse transcription (RT)-PCR a 524-nt fragment of the BYMV genome that includes the C-terminal 417 nt of the coat protein and 107 nt from the 3′ untranslated region. The IC-RT-PCR products were cloned into pGEM-T easy vector (Promega, Madison, WI) and a minimum of three clones from each PCR amplification were sequenced. BLAST analysis showed that the sequences of 30 samples were 96 to 98% identical to BYMV, but three samples (GenBank Accession Nos. EU860364–66) from bean, broad bean, and lentils had a high (98%) identity with Clover yellow vein virus (ClYVV). Sequence alignments of the ClYVV Spanish isolates and 14 ClYVV isolates from the GenBank (Accession Nos. AB03308, AB004545, AB011819, AF185959, AF203536, D86044, S77521, D95538–94) were obtained using the Clustal X software. Genetic distances were estimated using the Kimura two-parameter method. Within-population and between-population nucleotide diversities were estimated from the genetic distances (2). ClYVV sequences were phylogenetically separated into two clades: one with the three isolates from Japan (Accession Nos. D89542, D89543, and D89544) and the other with the remaining isolates. Molecular clustering coincides with biology and serological variations of strains 1 and 2 (3). Phylogenetic distances were independent of geographic origin, host, or time of sampling. The nucleotide diversity value among populations (0.18) was higher than within the subpopulations (0.017 and 0.029). dNS/dS in the ClYVV population was 0.031 (<1) and we can conclude that negative selection is occurring in the gene in study and that the population of ClYVV present in Spain is homogenous. In Spain, ClYVV was reported infecting borage (Borago officinalis L.) (1). To our knowledge, this is the first report of natural infection of bean, broad bean, and lentils with ClYVV in Spain. ClYVV might cause important economic losses in grain legumes since it causes an important viral disease of legumes worldwide. References: (1) M. Luis-Arteaga et al. Plant Pathol. 45:38, 1996. (2) M. Nei and T. Gojobori. Mol. Biol. Evol. 3:418, 1986. (3) T. Sasaya et al. Virology 87:1014, 1987.

scholarly journals Outbreak of Clover yellow vein virus in a Bean Field in Colusa County, California

Plant Disease ◽  
2001 ◽  
Vol 85 (4) ◽  
pp. 444-444 ◽  
Author(s):  
R. Crnov ◽  
R. L. Gilbertson
Keyword(s):  
Common Bean ◽  
Virus Disease ◽  
Sodium Dodecyl ◽  
Yellow Vein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Systemic Necrosis ◽  
First Report ◽  
Bean Plants ◽  
Clover Yellow Vein Virus

In 1999, a severe outbreak (i.e., 100% infection) of a virus disease was observed in a single field of common bean in Colusa County, CA. The symptoms included a yellow mosaic, leaf epinasty and, in some plants, a systemic necrosis. This field was adjacent to a clover field that had been harvested early in the development of the bean plants. A preliminary serological test (enzyme-linked immunosorbent assay, ELISA) suggested that the virus infecting these bean plants was Peanut mottle virus (PeMoV). This would represent the first report of this virus in California. A range of common bean cultivars (Black Turtle Soup, Topcrop, California Early Light Red Kidney, and Sutter Pink) were inoculated with sap prepared from symptomatic leaves collected from this field. Symptoms developing on these plants ranged from systemic necrosis (cvs. Sutter Pink and Black Turtle Soup) to strong yellow green mosaic and leaf distortion (cvs. Topcrop and California Early Light Red Kidney). Furthermore, inoculated primary leaves of cv. Topcrop failed to develop local lesions, which is characteristic of PeMoV. ELISAs on all symptomatic plants with antisera against PeMoV, BYMV, BCMV, and BCMNV as well as reverse transcription polymerase chain reaction (RT-PCR) analysis with primer pairs specific for PeMoV, BYMV, BCMV, and BCMNV were negative. To further investigate the nature of this virus, a minipurification method was used to purify virions from symptomatic leaves of all four cultivars. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis of purified virions from these cultivars revealed a 32-kDa band consistent with infection by a potyvirus. Transmission electron microscopy analysis of these preparations revealed the presence of potyvirus-like flexous rods (approximately 750 nm long and 10 nm wide). We next designed a primer pair specific for the coat protein gene of Clover yellow vein virus (ClYVV) and RT-PCR with these primers resulted in the amplification of a 630-bp DNA fragment from four isolates of the unknown potyvirus. No fragments were amplified from an uninfected control. The PCR-amplified fragments were direct-sequenced, and sequence comparisons revealed that the sequences of all four isolates were 95% identical to that of ClYVV (Genbank accession number D89541). Subsequently, a ClYVV antiserum was obtained from Simon Scott (Department of Plant Pathology, Clemson University), and ELISAs performed on leaves infected with all four isolates were positive. Finally, to assess whether the virus was seed-transmitted, seed harvested from this field was planted in a greenhouse (two lots of 400 seed each). None of the plants from these seeds developed virus symptoms, suggesting that the virus was not seed-transmitted. Together, these results indicate that the virus disease outbreak in this bean field was caused by ClYVV rather than PeMoV. The inoculum source for the virus was probably the adjacent clover field. This is the first report of ClYVV infecting common bean in California.

First report of leaf curling and yellowing caused by ageratum yellow vein virus in Phaseolus vulgaris in Okinawa Prefecture, Japan

2020 ◽  
Vol 86 (3) ◽  
pp. 227-232
Author(s):  
Yasuhiro Tomitaka ◽  
Ayako Yamaguchi ◽  
Sayumi Tanaka ◽  
Shinji Kawano ◽  
Yuna Tamayose ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Yellow Vein ◽  
First Report ◽  
Leaf Curling ◽  
Ageratum Yellow Vein Virus ◽  
Okinawa Prefecture

scholarly journals First report of ageratum yellow vein virus infecting tomato in Vietnam

2019 ◽  
Vol 101 (4) ◽  
pp. 1267-1267
Author(s):  
Hoseong Choi ◽  
Yeonhwa Jo ◽  
Phu-Tri Tran ◽  
Kook-Hyung Kim
Keyword(s):  
Yellow Vein ◽  
First Report ◽  
Ageratum Yellow Vein Virus

scholarly journals First Report of Ligustrum Virus A on Syringa reticulata var. mandshurica (Oleaceae) with a New Yellow Vein and Malformed Needle-Shaped Leaf Disease in China

Plant Disease ◽  
2018 ◽  
Vol 102 (10) ◽  
pp. 2053 ◽  
Author(s):  
T. Han ◽  
C. X. Yang ◽  
J. J. Fu ◽  
Q. S. Hou ◽  
S. Gang ◽  
...  
Keyword(s):  
Yellow Vein ◽  
First Report ◽  
Leaf Disease

scholarly journals The central and C-terminal domains of VPg of Clover yellow vein virus are important for VPg–HCPro and VPg–VPg interactions

2003 ◽  
Vol 84 (10) ◽  
pp. 2861-2869 ◽  
Author(s):  
Ma. Leonora M. Yambao ◽  
Chikara Masuta ◽  
Kenji Nakahara ◽  
Ichiro Uyeda
Keyword(s):  
Amino Acids ◽  
Western Blot ◽  
Yellow Vein ◽  
Transcription Activator ◽  
Yeast Two Hybrid ◽  
Yeast Two Hybrid System ◽  
Clover Yellow Vein Virus ◽  
Two Hybrid ◽  
Far Western Blot

Interactions between the major proteins of Clover yellow vein virus (ClYVV) were investigated using a GAL4 transcription activator-based yeast two-hybrid system (YTHS). Self-interactions manifested by VPg and HCPro and an interaction between NIb and NIaPro were observed in ClYVV. In addition, a strong HCPro–VPg interaction was detected by both YTHS and by in vitro far-Western blot analysis in ClYVV. A potyvirus HCPro–VPg interaction has not been reported previously. Using YTHS, domains in ClYVV for the VPg self-interaction and the HCPro–VPg interaction were mapped. The VPg C-terminal region (38 amino acids) was important for the VPg–VPg interaction and the central 19 amino acids were needed for the HCPro–VPg interaction.

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