scholarly journals Wheat: A New Natural Host for the Mal De Río Cuarto Virus in the Endemic Disease Area, Río Cuarto, Córdoba Province, Argentina

Plant Disease ◽  
1998 ◽  
Vol 82 (2) ◽  
pp. 149-152 ◽  
Author(s):  
P. E. Rodriguez Pardina ◽  
M. P. Giménez Pecci ◽  
I. G. Laguna ◽  
E. Dagoberto ◽  
G. Truol
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Wheat Crop ◽  
Enzyme Linked Immunosorbent Assay ◽  
Disease Area ◽  
Random Samples ◽  
Delphacodes Kuscheli ◽  
Preferred Species ◽  
First Time ◽  
Das Elisa

The fijivirus known as “Mal de Río Cuarto” that affects corn is endemic to the area of Río Cuarto, Cordoba Province, Argentina. One of the preferred species for the development of its vector, the insect Delphacodes kuscheli Fennah, is wheat. In this area, wheat plants with deformed leaves, spikes and spikelets, shortened internodes, leaves with serrated borders, and sterile spikelets were detected, suggesting the possibility that Mal de Río Cuarto Virus could also be infecting this crop. Samples originating in Río Cuarto, Sampacho, and La Carlota (Córdoba Province) that showed symptoms, were analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), polyacrylamide gel electrophoresis, and electron microscopy, confirming, for the first time, the occurrence of the disease in wheat. The frequency of the disease was assessed in random samples from 14 wheat plots located in the Department of Río Cuarto (Córdoba Province). The samples were analyzed using the DASELISA immunoenzymatic technique, and the disease was detected in the majority of the fields assessed, with levels of incidence that ranged between 2.5 and 24%. We must be aware of the presence of this virus in the wheat crop, where it appears to play a double role in the epidemiology of the disease, acting both as a virus reservoir and as a preferred host for the development of populations of the vector virus, D. kuscheli.

scholarly journals Variations of Leek yellow stripe virus Concentration in Garlic and Its Incidence in Argentina

Plant Disease ◽  
2002 ◽  
Vol 86 (10) ◽  
pp. 1085-1088 ◽  
Author(s):  
Vilma C. Conci ◽  
Pablo Lunello ◽  
Diana Buraschi ◽  
Rusell R. Italia ◽  
Sergio F. Nome
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Concentration ◽  
Santa Cruz ◽  
Leek Yellow Stripe Virus ◽  
Random Samples ◽  
Crop Cycle ◽  
White Type ◽  
Yellow Stripe ◽  
Das Elisa

The purpose of this work was to determine variations in titer of Leek yellow stripe virus (LYSV) throughout the crop cycle and bulb storage, and to evaluate the incidence of infected plants in the main garlic-production regions of Argentina. One hundred plants with LYSV from each of five cultivars were analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) in six different vegetative stages in White- and Red-type garlic cultivars and seven stages in cv. Rosado Paraguayo, throughout the year. In two White-type garlic cultivars, LYSV showed peaks of viral concentration in May, at the beginning of the crop cycle, and in November, just before harvest. In two Red-type garlic selections, an increase was detected in November (period of bulbing). The highest virus titers for these four garlic cultivars were detected in devernalized clove. In Rosado Paraguayo, the peak virus concentration occurred in September prior to harvesting. In a survey at 14 different localities in Argentina, 3,066 random samples were analyzed. LYSV was found in 80 to 98% of the plants from all regions, except in Santa Cruz, where 34% of plants were infected. The importance of this study is that it allows us to recommend the most suitable moment of the year to make the analysis with DAS-ELISA.

scholarly journals Antibody produced against isolated Rh(D) polypeptide reacts with other Rh-related antigens

Blood ◽  
1988 ◽  
Vol 72 (5) ◽  
pp. 1622-1626
Author(s):  
K Suyama ◽  
J Goldstein
Keyword(s):  
Gel Electrophoresis ◽  
High Titer ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rabbit Antibody ◽  
Immune Precipitation ◽  
D Cells ◽  
D Antigen ◽  
Related Proteins

Rh(D) antigen-containing polypeptide was prepared by immune precipitation of intact cDE/cDE erythrocytes by using a high-titer preparation of polyclonal anti-D. when isolated Rh(D) polypeptide was administered to rabbits, antibody was produced that was unresponsive toward Rh-positive and -negative cells but reacted strongly with the immunogen in enzyme-linked immunosorbent assay-type immunobinding and Western blot immunostaining assays. Rabbit antibody also immunostains isolated Rh(c) polypeptide as well as the Rh antigen-containing components of sodium dodecyl sulfate-polyacrylamide gel electrophoresis- separated membrane proteins from Rh(D)-positive (cDE/cDE,CDe/CDe), Rh(D)-negative (cde/cde,Cde/Cde), and -D-/-D- cells. It does not react with any membrane protein from Rh-null regulator type cells, thus indicating a specificity for Rh-related proteins. We have also been able to demonstrate that polyclonal and monoclonal anti-D preparations that do not immunostain isolated Rh(D) polypeptide will react with it in our immunobinding assay.

scholarly journals Allozyme pattern for new record of Craspedacusta sowerbii in Serbia

2004 ◽  
pp. 15-19 ◽  
Author(s):  
Jasmina Ludoski ◽  
Vesna Milankov ◽  
Predrag Radisic
Keyword(s):  
Genetic Variability ◽  
Natural Population ◽  
Gel Electrophoresis ◽  
New Record ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Serbia And Montenegro ◽  
Enzyme Loci ◽  
First Time

Cosmopolitan freshwater jellyfish Craspedacusta sowerbii L a n k e s t e r 1880 (Cnidaria, Hydrozoa) was recorded for the first time in the lake Velika peskara near Zrenjanin (Serbia and Montenegro) in summer 1998. A natural population of C. sowerbii from the lake Velika peskara was analyzed for genetic variability at 9 enzyme loci (Gpi, Hk, Idh-1, Idh-2, Me, Mdh-1 Mdh-2, Pgm and Sod) by polyacrylamide gel electrophoresis. A zymogram indicated that population was monomorphic at all analyzed loci.

Using Cell-Based Activity Assays In Support Of Carryover Calculations In Multi-Product Facilities

2021 ◽  
Vol 27 (4) ◽  
Author(s):  
Gabriella del Hierro ◽  
Emily Holz ◽  
Ed Contreras ◽  
Pauline Che ◽  
Shelley Elvington ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Immunosorbent Assay ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Analytical Techniques ◽  
Polyacrylamide Gel ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Cell ◽  
Therapeutic Antibodies

The calculation of cleaning carryover limits in multi-product facilities can be based on the inactivity of molecules after exposure to cleaning conditions if the inactivation of active molecules can be demonstrated. The demonstration of inactivation has been addressed in several publications that have shown degradation and/or denaturation using different analytical techniques such as sodium dodecyl sulfate-polyacrylamide gel electrophoresis and enzyme-linked immunosorbent assay, which directly or indirectly demonstrate that the product residue is no longer active. In this paper, authors expand the assay options by demonstrating the use of molecule-specific cell-based activity assays, which provide a “catch all” measurement of sample bioactivity, to assess the inactivation of therapeutic antibodies after exposure to cleaning conditions.

PAP, a pancreatic secretory protein induced during acute pancreatitis, is expressed in rat intestine

1993 ◽  
Vol 265 (4) ◽  
pp. G611-G618 ◽  
Author(s):  
J. L. Iovanna ◽  
V. Keim ◽  
A. Bosshard ◽  
B. Orelle ◽  
J. M. Frigerio ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Secretory Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rat Intestine ◽  
Mrna Concentration ◽  
Initial Content ◽  
Pancreatitis Associated Protein

The pancreatitis-associated protein (PAP) is a lectin-related secretory protein present in small amounts in the rat pancreas and rapidly overexpressed during the acute phase of pancreatitis. We demonstrate in this report that PAP is also expressed in rat intestine. A cDNA library from rat jejunum was probed with pancreatic PAP cDNA. The inserts of the selected recombinant clones corresponded to a transcript whose nucleotide sequence was identical to that of pancreatic PAP mRNA. The transcript was detected in duodenum, jejunum, ileum, and colon. A protein with same molecular mass (16 kDa) and pI (8.2) as pancreatic PAP was actually immunodetected in ileum homogenate after separation by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Intestinal PAP was immunolocalized to the epithelial cells of the lower part of the villi. The protein accounted respectively for 0.02, 0.05, and 0.1% of soluble proteins in duodenum, jejunum, and ileum homogenates, as measured by enzyme-linked immunosorbent assay, and could not be detected in stomach and colon. Influence of fasting and feeding on PAP mRNA concentration was analyzed in ileum. Concentration decreased by 81 and 94% after animals were fasted for 24 and 48 h, respectively. Feeding restored the initial content within 6 h. On the other hand, intestinal PAP mRNA concentration was not altered during acute pancreatitis

scholarly journals Identification of the Wheat Curl Mite as the Vector of the High Plains Virus of Corn and Wheat

Plant Disease ◽  
1997 ◽  
Vol 81 (10) ◽  
pp. 1161-1166 ◽  
Author(s):  
Dallas L. Seifers ◽  
Tom L. Harvey ◽  
T. J. Martin ◽  
Stanley G. Jensen
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Triticum Aestivum L ◽  
Enzyme Linked Immunosorbent Assay ◽  
High Plains ◽  
Serological Relationship ◽  
Hordeum Vulgare L ◽  
Wheat Curl Mite ◽  
Aceria Tosichella

Wheat with virus-like symptoms (extracts containing a 33-kDa protein in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, negative in enzyme-linked immunosorbent assay to wheat streak mosaic virus, and not infectious in a backassay to other wheat) reacted positively to antiserum made against a protein purified from symptomatic corn infected with the High Plains virus (HPV), indicating a serological relationship between the corn and wheat pathogens. The wheat curl mite (WCM, Aceria tosichella Keifer) was identified as the vector of the virus and caused persistent infection of barley (Hordeum vulgare L.) and wheat (Triticum aestivum L.) in greenhouse experiments. The HPV was recovered in the field from naturally infected wheat where the number of HPV-infected plants decreased with increasing distance from the WCM source in volunteer wheat.

Sign in / Sign up

Export Citation Format

Share Document