scholarly journals Variations of Leek yellow stripe virus Concentration in Garlic and Its Incidence in Argentina

Plant Disease ◽  
2002 ◽  
Vol 86 (10) ◽  
pp. 1085-1088 ◽  
Author(s):  
Vilma C. Conci ◽  
Pablo Lunello ◽  
Diana Buraschi ◽  
Rusell R. Italia ◽  
Sergio F. Nome
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Concentration ◽  
Santa Cruz ◽  
Leek Yellow Stripe Virus ◽  
Random Samples ◽  
Crop Cycle ◽  
White Type ◽  
Yellow Stripe ◽  
Das Elisa

The purpose of this work was to determine variations in titer of Leek yellow stripe virus (LYSV) throughout the crop cycle and bulb storage, and to evaluate the incidence of infected plants in the main garlic-production regions of Argentina. One hundred plants with LYSV from each of five cultivars were analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) in six different vegetative stages in White- and Red-type garlic cultivars and seven stages in cv. Rosado Paraguayo, throughout the year. In two White-type garlic cultivars, LYSV showed peaks of viral concentration in May, at the beginning of the crop cycle, and in November, just before harvest. In two Red-type garlic selections, an increase was detected in November (period of bulbing). The highest virus titers for these four garlic cultivars were detected in devernalized clove. In Rosado Paraguayo, the peak virus concentration occurred in September prior to harvesting. In a survey at 14 different localities in Argentina, 3,066 random samples were analyzed. LYSV was found in 80 to 98% of the plants from all regions, except in Santa Cruz, where 34% of plants were infected. The importance of this study is that it allows us to recommend the most suitable moment of the year to make the analysis with DAS-ELISA.

scholarly journals Changes in the Concentration of an Allexivirus During the Crop Cycle of Two Garlic Cultivars

Plant Disease ◽  
2006 ◽  
Vol 90 (10) ◽  
pp. 1293-1296 ◽  
Author(s):  
Eva E. Cafrune ◽  
Mónica Balzarini ◽  
Vilma C. Conci
Keyword(s):  
Virus Titer ◽  
Virus Detection ◽  
Coat Protein Sequence ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Concentration ◽  
Accession Number ◽  
Crop Cycle ◽  
Garlic Virus ◽  
Sampling Times ◽  
Das Elisa

Garlic can be infected by a number of viruses, including allexiviruses. The coat protein sequence of an Allexivirus was detected in Argentina and deposited in the EMBL database as Garlic mite-borne filamentous virus (accession number X98991); it has high homology with Garlic virus A (GarV-A). For reliable virus detection, plants should be sampled when virus titer is high to reduce the risk of identifying infected plants as healthy. The objective of this study was to describe fluctuations in the concentration of this Argentine isolate of GarV-A in two garlic cultivars, Morado-INTA and Nieve-INTA, throughout the crop cycle using the double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Over a 2-year period, for both cultivars, virus concentration was assessed in samples from the tips section of the youngest leaves of GarV-A-infected plants, and from basal sections of both dormant and devernalized cloves of stored bulbs of Morado-INTA. The concentration of GarV-A varied during the crop cycle, but peaked at the beginning and again at the end of the crop cycle. Virus concentration was slightly higher in devernalized cloves compared with dormant cloves of Morado-INTA. No correlation between virus concentration and mean air temperature was observed. The results of this study recommend sampling times at the beginning of the crop cycle at 64 to 81 days after planting, and towards the end of the crop cycle to evaluate for the presence of GarV-A by DAS-ELISA.

scholarly journals Insiden Penyakit Virus Tular Umbi pada Tigabelas Varietas Bawang Merah Asal Jawa Barat dan Jawa Tengah

2016 ◽  
Vol 21 (2) ◽  
pp. 164
Author(s):  
Neni Gunaeni ◽  
Astri W Wulandari ◽  
Ati Srie Duriat ◽  
Agus Muharam
Keyword(s):  
Immunosorbent Assay ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Diseases ◽  
Double Antibody ◽  
Yellow Dwarf Virus ◽  
Yellow Stripe ◽  
Das Elisa

Penyakit virus tular umbi merupakan salah satu kendala dalam meningkatkan produksi bawang merah. Hal ini disebabkan oleh virus yang infeksinya bersifat sistemik. Apabila partikel virus berada dalam jaringan benih umbi, maka akan sulit untuk dikendalikan dan dapat membawa masalah baru pada pertanaman berikutnya. Penelitian bertujuan mengetahui insiden penyakit virus tular umbi pada 13 varietas bawang merah yang berasal dari Jawa Barat dan Jawa Tengah. Penelitian dilaksanakan di Rancaekek (elevasi 650 m dpl.) dan di Laboratorium Balai Penelitian Tanaman Sayuran Lembang (elevasi 1.250 m dpl.), sejak bulan Agustus sampai November 2004. Perlakuan terdiri atas 13 varietas bawang merah, yaitu: Lodra, Sumenep, Batu, Merah Maja, Merah Cigugur, Ciniru, Bima, Bima Curut, Bima Timor, Bima Arjuna, Kuning Tablet, Kuning Gombong, dan Philipina. Rancangan yang digunakan ialah acak kelompok dengan tiga ulangan. Hasil penelitian menunjukkan bahwa (1) insiden penyakit virus tular umbi pada masing-masing varietas bawang merah asal Jawa Barat dan Jawa Tengah berturut-turut yaitu varietas Lodra 84,67%, Sumenep 82,56-100%, Batu 39,86-78,67%, Merah Maja 95,25%, Merah Cigugur 100%, Ciniru 66,27%, Bima Curut 78,57%, Bima 100%, Bima Timor 57,98%, Bima Arjuna 47,96%, Kuning Tablet 57,48%, Kuning Gombong 97,92%, dan Philipina 97,92 %, (2) gejala infeksi virus pada daun umumnya berupa  klorosis, mosaik bergaris kuning vertikal terputus-putus, garis-garis hijau vertikal, dan ukuran daun menjadi kecil, (3) gejala-gejala tersebut bereaksi positif dengan OYDV(onion yellow dwarf virus) dan SYSV (shallot yellow stripe virus) berdasarkan uji DAS-ELISA (double antibody sandwich-enzyme linked immunosorbent assay). Informasi mengenai insiden virus tular umbi pada bawang merah ini sangat penting dalam rangka mengembangkan metode perbenihan bawang merah bebas virus. <br /><br />Virus disease is one of major problems in increasing shallots production, because its infection has a systemic character. If it is already in shallots bulb tissues, the virus is difficult to be controlled and will cause new problems to the next planting. The experiment was aimed to determine incidence of bulb-borne virus diseases on  thirteen varieties of shallots (Allium cepa var. ascalonicum) originated from West  and Central Java. The experiment was carried out at Indonesian Vegetable Research Institute Lembang (1,250 m asl.) and Rancaekek (650 m asl.), from  August to November 2004. The shallot varieties tested were Lodra, Sumenep, Batu, Merah Maja, Merah Cigugur, Ciniru, Bima, Bima Curut, Bima Timor, Bima Arjuna, Kuning Tablet, Kuning Gombong, and Philipina. A randomized complete block design with three replications were used in this experiment. The results of the experiment showed that  (1) incidence of virus diseases in shallots bulb on variety Lodra was 84.67%, Sumenep 82.56-100%, Batu 39.86-78.67%, Merah Maja 95.25%, Merah Cigugur 100%, Ciniru 66.27%, Bima Curut 78.57%, Bima 100%, Bima Timor 57.98%, Bima Arjuna 47.96%, Kuning Tablet 57.48%, Kuning Gombong 97.92%,  and Philipina 97.92 %, (2) the virus symptoms exhibited on infected shallots were  yellow stripe mosaic, chlorosis,  green stripe leaf,  and leaves became small, and (3) the symptoms were associated with OYDV (onion yellow dwarf virus) and SYSV (shallots yellow stripe virus) base on DAS-ELISA (double antibody sandwich-enzyme linked immunosorbent assay). Information on the incidence of viral diseases on shallots bulb is very important to develop the production technology of virus-free shallots bulb.<br /><br /><br />

scholarly journals Distribution of viruses in the shallot germplasm collection of the Czech Republic – Short Communication

2017 ◽  
Vol 44 (No. 1) ◽  
pp. 49-52 ◽  
Author(s):  
Kateřina Smékalová ◽  
Helena Stavělíková ◽  
Karel Dušek
Keyword(s):  
Germplasm Collection ◽  
Latent Virus ◽  
Onion Yellow Dwarf Virus ◽  
The Czech Republic ◽  
Leek Yellow Stripe Virus ◽  
Garlic Common Latent Virus ◽  
Shallot Latent Virus ◽  
Yellow Dwarf Virus ◽  
Yellow Stripe ◽  
Das Elisa

The Czech collection of shallot (Allium cepa var. ascalonicum) genetic resources (122 accessions) was surveyed for the presence of four different viruses, i.e. Onion yellow dwarf virus, Leek yellow stripe virus, Garlic common latent virus (GCLV), and Shallot latent virus, by DAS-ELISA. The shallot seems to be resistant against GCLV because none of the tested plants was infested by this virus. Other three viruses were found with an incidence ranging from 53% to 93% for genotypes and 48% to 87% for plants. Most of the tested shallot genotypes were simultaneously infected with two or three viruses. These results were compared with neighbouring collection of garlic where all four viruses were found widespread with an incidence ranging from 65% to 83% for genotypes and 39% to 61% for plants.  

scholarly journals Decision Strategies for Absorbance Readings from an Enzyme-Linked Immunosorbent Assay—A Case Study about Testing Genotypes of Sugar Beet (Beta vulgaris L.) for Resistance against Beet Necrotic Yellow Vein Virus (BNYVV)

Agriculture ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 956
Author(s):  
Thomas M. Lange ◽  
Martin Wutke ◽  
Lisa Bertram ◽  
Harald Keunecke ◽  
Friedrich Kopisch-Obuch ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Beta Vulgaris ◽  
Immunosorbent Assay ◽  
Area Under The Curve ◽  
Bayes Factors ◽  
Yellow Vein ◽  
Small Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Concentration ◽  
Alternative Approach

The Beet necrotic yellow vein virus (BNYVV) causes rhizomania in sugar beet (Beta vulgaris L.), which is one of the most destructive diseases in sugar beet worldwide. In breeding projects towards resistance against BNYVV, the enzyme-linked immunosorbent assay (ELISA) is used to determine the virus concentration in plant roots and, thus, the resistance levels of genotypes. Here, we present a simulation study to generate 10,000 small samples from the estimated density functions of ELISA values from susceptible and resistant sugar beet genotypes. We apply receiver operating characteristic (ROC) analysis to these samples to optimise the cutoff values for sample sizes from two to eight and determine the false positive rates (FPR), true positive rates (TPR), and area under the curve (AUC). We present, furthermore, an alternative approach based upon Bayes factors to improve the decision procedure. The Bayesian approach has proven to be superior to the simple cutoff approach. The presented results could help evaluate or improve existing breeding programs and help design future selection procedures based upon ELISA. An R-script for the classification of sample data based upon Bayes factors is provided.

scholarly journals Wheat: A New Natural Host for the Mal De Río Cuarto Virus in the Endemic Disease Area, Río Cuarto, Córdoba Province, Argentina

Plant Disease ◽  
1998 ◽  
Vol 82 (2) ◽  
pp. 149-152 ◽  
Author(s):  
P. E. Rodriguez Pardina ◽  
M. P. Giménez Pecci ◽  
I. G. Laguna ◽  
E. Dagoberto ◽  
G. Truol
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Wheat Crop ◽  
Enzyme Linked Immunosorbent Assay ◽  
Disease Area ◽  
Random Samples ◽  
Delphacodes Kuscheli ◽  
Preferred Species ◽  
First Time ◽  
Das Elisa

The fijivirus known as “Mal de Río Cuarto” that affects corn is endemic to the area of Río Cuarto, Cordoba Province, Argentina. One of the preferred species for the development of its vector, the insect Delphacodes kuscheli Fennah, is wheat. In this area, wheat plants with deformed leaves, spikes and spikelets, shortened internodes, leaves with serrated borders, and sterile spikelets were detected, suggesting the possibility that Mal de Río Cuarto Virus could also be infecting this crop. Samples originating in Río Cuarto, Sampacho, and La Carlota (Córdoba Province) that showed symptoms, were analyzed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), polyacrylamide gel electrophoresis, and electron microscopy, confirming, for the first time, the occurrence of the disease in wheat. The frequency of the disease was assessed in random samples from 14 wheat plots located in the Department of Río Cuarto (Córdoba Province). The samples were analyzed using the DASELISA immunoenzymatic technique, and the disease was detected in the majority of the fields assessed, with levels of incidence that ranged between 2.5 and 24%. We must be aware of the presence of this virus in the wheat crop, where it appears to play a double role in the epidemiology of the disease, acting both as a virus reservoir and as a preferred host for the development of populations of the vector virus, D. kuscheli.

scholarly journals EFICIÊNCIA NA DETECÇÃO DOS VÍRUS PLRV, PVX E PVY EM TECIDOS VEGETAIS DE GEMA APICAL DORMENTE MAIS ESTOLÃO, BROTOS E FOLHAS DA BATATA, PELO MÉTODO SOROLÓGICO ENZYME-LINKED IMMUNOSORBENT ASSAY-ELISA

2000 ◽  
Vol 1 (1) ◽  
pp. 80 ◽  
Author(s):  
Milton Vasconcelos GUEDES
Keyword(s):  
Solanum Tuberosum ◽  
Immunosorbent Assay ◽  
Solanum Tuberosum L ◽  
Enzyme Linked Immunosorbent Assay ◽  
De Se ◽  
Das Elisa

A propagação vegetativa da batata (Solanum tuberosum L.) é a causa da degenerescência de variedades uma vez que o tubérculo-semente utilizado no plantio pode perpetuar os vírus incidentes na planta-mãe. Daí a necessidade de renovação constante de estoques de batata-semente, bem como, de se obter técnicas eficientes de diagnóstico. Os objetivos gerais deste trabalho foram (1) comparar a eficiência na detecção dos vírus PLRV, PVX e PVY por meio do teste sorológico DAS-ELISA em tecidos vegetais de diferentes origens:- da gema apical + estolão, brotos e folhas, após quebra natural de dormência, das variedades Baraka, Contenda e Elvira; (2) verificar a confiabilidade da detecção de vírus em tecidos vegetais dormentes ou récem-brotados, visando reduzir o tempo de obtenção dos resultados em relação ao uso de folhas. Os objetivos específicos foram: avaliar a incidência dos diferentes vírus em amostras de campos de batata-consumo, na segunda geração de multiplicação; comparar os resultados obtidos com o equipamento de leitura de ELISA e os da leitura visual. A homogeneidade das variâncias dos tratamentos foi testada pelo teste de Bartlett e a comparação das médias dos tratamentos foi feita utilizando-se o teste de Tukey, ambos ao nível de 5% de probabilidade. Os resultados foram analisados utilizando-se o programa estatístico MSTATC, versão 2.11. A incidência média das diferentes viroses nas amostras das variedades Baraka, Contenda e Elvira, foi maior para PVY e PLRV, e muito baixa para PVX. O PVY foi o vírus de maior incidência nas três variedades: 14,1% em Baraka, 10,9% em Elvira e 2,9% em Contenda. O PLRV ocorreu com incidência de 3,6% na variedade Elvira, 2,3% em Baraka e 0,7% em Contenda. O PVX foi detectado em níveis inferiores a 0,5% nas três variedades; a variedade Contenda foi a que apresentou os menores índices médios de incidência para os três vírus, quando comparados às demais variedades estudadas; o teste DAS-ELISA pode ser utilizado na detecção dos vírus PLRV e PVX, tanto em gema apical dormente + estolão, como em brotos ou folhas, após quebra natural de dormência nas variedades Baraka, Contenda e Elvira; a eficiência do teste DAS ELISA pode variar de acordo com a origem do tecido vegetal utilizado; o vírus PVY, não pode ser detectado com segurança na gema apical dormente + estolão, para as três variedades testadas. Tendo em vista os resultados apresentados pode-se concluir que a detecção das viroses em brotos foi mais eficiente para todas as variedades estudadas antecipando-se os resultados e proporcionando um ganho de no mínimo 30 dias.

scholarly journals First Report of Leek yellow stripe virus in Garlic in the State of Guanajuato, Mexico

Plant Disease ◽  
2006 ◽  
Vol 90 (11) ◽  
pp. 1458-1458 ◽  
Author(s):  
L. Pérez-Moreno ◽  
Z. Córdova-Rosales ◽  
E. Barboza-Corona ◽  
Rafael Ramírez-Malagón ◽  
J. Ramírez-Lúa ◽  
...  
Keyword(s):  
Coat Protein ◽  
Blot Analysis ◽  
The State ◽  
Latent Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
First Report ◽  
Leek Yellow Stripe Virus ◽  
Virus Complex ◽  
Yellow Stripe ◽  
Rna Blot Analysis

Garlic (Allium sativum L.) can be affected by a virus complex (1) consisting of two potyviruses, Onion yellow dwarf virus (OYDV) and Leek yellow stripe virus (LYSV), and two carlaviruses, Garlic common latent virus (GCLV) and Shallot latent virus (SLV) (1). To identify the components of the virus complex that could be present in garlic plants in Guanajuato State, which is the second largest garlic producer in the country and where presumptive viral symptoms were initially observed in December 2004, a survey was carried out in six locations: San Miguel de Allende and San Luis de la Paz in northern Guanajuato; Irapuato and Villagrán in the central region; and Salamanca and Valle de Santiago in the southern part of the state. Enzyme-linked immunosorbent assay (ELISA) was carried out to detect LYSV, OYDV, GCLV, and SLV in 195 garlic leaf samples collected during January 2005 from plants with leaf yellow stripe, mosaic, enation, deformation, or dwarfism symptoms. A set of primers, previously reported and specific to the coat protein cistron of LYSV (1), were synthesized and used in a reverse transcriptase-polymerase chain reaction (RT-PCR). The amplified product (1,020 nucleotides) was cloned into plasmid pGEM T-Easy (Promega, Madison, WI) and sequenced (Gen-bank Accession No. DQ841554). Sequence analysis showed that the cloned DNA fragment shared 97% similarity with the coat protein cistron of LYSV isolate no. 3 from Okinawa (GenBank Accession No. AB194632). The fragment was then radioactively labelled and used as a probe in the RNA blot analysis of all samples to confirm the ELISA results of LYSV. Of the 195 samples, 64 tested positive by RNA blot analysis. Forty-one of these were also positive by ELISA for LYSV. Preliminary, positive ELISA results were also obtained for OYDV, GCLV and SLV. To our knowledge, this is the first report of LYSV in the State of Guanajuato and in Mexico. The correct identification of viruses present in garlic will help to use the appropriate strategies to reduce viral incidence in this garlic-producing region. Reference: (1) T. V. M. Fajardo et al. Fitopatol. Bras. 26:619, 2001.

scholarly journals Distribución viral en plantas de cebolla (Allium cepa L.) asintomáticas

2018 ◽  
Vol 3 (7) ◽  
pp. 1425-1434 ◽  
Author(s):  
Rodolfo Velásquez-Valle ◽  
Manuel Reveles-Hernández ◽  
Mario Domingo Amador-Ramírez
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Onion Yellow Dwarf Virus ◽  
Leek Yellow Stripe Virus ◽  
Tomato Spotted Wilt ◽  
Allium Cepa L ◽  
Garlic Common Latent Virus ◽  
Yellow Dwarf Virus ◽  
Yellow Stripe ◽  
Wilt Virus ◽  
Das Elisa

Se estudió por medio de DAS- ELISA la distribución del Onion yellow dwarf virus (OYDV, virus del enanismo amarillo de la cebolla), Garlic common latent virus (GarCLV, virus latente común del ajo), Shallot latent v irus (SLV, virus latente del chalote), Leek yellow stripe virus (LYSV: virus de la franja amarilla del puerro), Tobacco etch virus (TEV, virus del jaspeado del tabaco) y Tomato spotted wilt virus (TSWV, virus de la marchitez manchada del jitomate) en hojas, psdeudotallo e inflorescencia de plantas de cebolla asintomáticas colectadas en diferentes épocas de 2010 en tres localidades del estado de Zacatecas, México. Se registró la presencia de esos virus en los órganos mencionados aunque resultó frecuente detectar infecciones mezcladas en hojas individuales donde las interacciones más comunes involucraban dos, tres y cuatro virus. No se encontró una tendencia que relacione la presencia viral con los órganos vegetales analizados o con la edad de las hojas; sin embargo, las infecciones con un solo virus parecen ser menos frecuentes en las hojas de edad intermedia.

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