scholarly journals First Report of Zucchini yellow mosaic virus in Argentina

Plant Disease ◽  
2000 ◽  
Vol 84 (3) ◽  
pp. 371-371 ◽  
Author(s):  
O. Gracia
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Disease Incidence ◽  
Sodium Dodecyl ◽  
Zucchini Yellow Mosaic Virus ◽  
Chenopodium Quinoa ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Mechanical Transmission ◽  
Chenopodium Amaranticolor

In August 1996, a severe viral disease occurred in squash produced in the subtropical Province of Salta, Argentina. Plants of Cucurbita pepo L. (zucchini) and Cucurbita maxima L. ‘Any’ were affected. Approximately 50% of the plants showed prominent yellow mosaic, necrosis, and foliar distortion. Most of the fruits on infected plants were small, with scattered glossy yellow knobs over a green background, and some showed additional fruit malformation. A potyvirus was isolated from infected plants by mechanical transmission. Filamentous particles were associated with symptomatic inoculated squash and cucumber plants in leaf-dip preparations with an electron microscope (Elmisckop I, Siemens, Germany). The particles were flexuous rods ≈755 nm long. The host range of the isolated virus was mostly limited to the cucurbits (systemic infection) but also included Gomphrena globosa (local and systemic symptoms) and Chenopodium quinoa and Chenopodium amaranticolor, which showed only local chlorotic lesions. Twenty species in the Compositae, Leguminosae, and Solanaceae were not infected (2). In agar double-diffusion tests with sodium dodecyl sulfate (SDS)-degraded virus particles, no reactions were observed with Papaya ringspot virus, Watermelon mosaic virus (WMV), and WMV-Mo antisera, but a strong precipitin line was obtained with the antiserum prepared by Purcifull (1) for isolate 1119 of Zucchini yellow mosaic virus (ZYMV). Isolate 1119 and our isolate appeared serologically indistinguishable in SDS immunodiffusion tests. Both gave fused precipitin bands without spur formation. The results (field symptoms, transmissibility, host range, particle morphology, and serology) lead to the conclusion that ZYMV is present in Argentina. Since 1996, outbreaks have occurred every year in Salta, devastating squash crops. In 1998, ZYMV also was found in the provinces of Mendoza and San Juan, infecting zucchini squash and melons (Cucumis melo L.). A survey of crops indicates that disease incidence and severity were lower than in Salta. References: (1) D. E. Purcifull et al. Plant Dis. 68:230, 1984. (2) H. L. Wang. Plant Dis. 76:530, 1992.

scholarly journals WATERMELON MOSAIC VIRUS OF PUMPKIN (Cucurbita maxima) FROM SULAWESI: IDENTIFICATION, TRANSMISSION, AND HOST RANGE

2016 ◽  
Vol 3 (1) ◽  
pp. 33
Author(s):  
Wasmo Wakmana ◽  
M.S. Kontong ◽  
D.S. Teakle ◽  
D.M. Persley
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Disease Transmission ◽  
Zucchini Yellow Mosaic Virus ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Cucurbita Maxima ◽  
Mechanical Inoculation ◽  
Water Melon

A mosaic disease of pumpkin (Cucurbita maxima) was spread widely in Sulawesi. Since the virus had not yet been identified, a study was conducted to identify the disease through mechanical inoculation, aphid vector transmission, host range, and electron microscopic test. Crude sap of infected pumpkin leaf samples was rubbed on the cotyledons of healthy pumpkin seedlings for mechanical inoculation. For insect transmission, five infective aphids were infected per seedling. Seedlings of eleven different species were inoculated mechanically for host range test. Clarified sap was examined under the electron microscope. Seeds of two pumpkin fruits from two different infected plants were planted and observed for disease transmission up to one-month old seedlings. The mosaic disease was transmitted mechanically from crude sap of different leaf samples to healthy pumpkin seedlings showing mosaic symptoms. The virus also infected eight cucurbits, i.e., cucumber (Cucumis sativus), green melon (Cucumis melo), orange/rock melon (C. melo), zucchini (Cucurbita pepo), pumpkin (Cucurbita maxima), water melon (Citrulus vulgaris), Bennicosa hispida, and blewah (Cucurbita sp.). Aphids  transmitted the disease from one to other pumpkin seedlings. The virus was not transmitted by seed. The mosaic disease of pumpkin at Maros, South Sulawesi, was associated with flexious particles of approximately 750 nm length, possibly a potyvirus, such as water melon mosaic virus rather than papaya ringspot virus or zucchini yellow mosaic virus.

scholarly journals Cayaponia tibiricae: New Host of Zucchini Yellow Mosaic Virus in Brazil

Plant Disease ◽  
1999 ◽  
Vol 83 (5) ◽  
pp. 486-486 ◽  
Author(s):  
V. A. Yuki ◽  
J. A. M. Rezende ◽  
E. W. Kitajima ◽  
P. A. V. Barroso ◽  
H. Kuniyuki ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Electron Microscopic Examination ◽  
Yellow Mosaic Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Secondary Forests ◽  
Electron Microscopic ◽  
Chenopodium Amaranticolor ◽  
New Host

Cayaponia tibiricae Cogn. (CT) is a wild Cucurbitaceae species found in secondary forests in the State of São Paulo, Brazil. The species has indefinite growth and bears oblong dark green fruits, 15 to 20 mm long (1,2). CT plants showing yellow mosaic symptoms were found in Atibaia County. Extracts from symptomatic plants were rub inoculated to zucchini squash (Cucurbita pepo L.) and Chenopodium amaranticolor Coste & Reyn. Zucchini squash plants developed severe yellow mosaic with intense leaf malformation, while C. amaranticolor showed necrotic local lesions. Extracts from naturally infected CT, zucchini squash, and C. amaranticolor were tested by plate trapped antigen-enzyme-linked immunosorbent assay (PTA-ELISA) with antisera against papaya ringspot virus type W (PRSV-W), zucchini yellow mosaic virus (ZYMV), zucchini lethal chlorosis virus (ZLCV), watermelon mosaic virus 2 (WMV-2), and cucumber mosaic virus (CMV). All samples were positive in PTA-ELISA only with ZYMV antiserum. Also, in Western blot (immunoblot) assay, ZYMV antiserum labeled a protein of approximately 36 kDa. Electron microscopic examination of ultrathin sections from infected CT tissue revealed the presence of pinwheel inclusions typical of potyvirus (type 1) infection in the cytoplasm of the cell. CT seedlings were susceptible to mechanical inoculation with the ZYMV isolated from this species. This is the first report of CT as a natural host for ZYMV in Brazil. References: (1) A. Cogniaux. Flora Brasiliensis 6:1, 1878. (2) M. Pio Corrêa. 1926. Diccionário das plantas úteis do Brasil e das exóticas cultivadas. Vol 1. Ministério da Agricultura, Rio de Janeiro, Brazil.

scholarly journals WATERMELON MOSAIC VIRUS OF PUMPKIN (Cucurbita maxima) FROM SULAWESI: IDENTIFICATION, TRANSMISSION, AND HOST RANGE

2016 ◽  
Vol 3 (1) ◽  
pp. 33 ◽  
Author(s):  
Wasmo Wakmana ◽  
M.S. Kontong ◽  
D.S. Teakle ◽  
D.M. Persley
Keyword(s):  
Host Range ◽  
Mosaic Virus ◽  
Disease Transmission ◽  
Zucchini Yellow Mosaic Virus ◽  
Mosaic Disease ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Cucurbita Maxima ◽  
Mechanical Inoculation ◽  
Water Melon

A mosaic disease of pumpkin (Cucurbita maxima) was spread widely in Sulawesi. Since the virus had not yet been identified, a study was conducted to identify the disease through mechanical inoculation, aphid vector transmission, host range, and electron microscopic test. Crude sap of infected pumpkin leaf samples was rubbed on the cotyledons of healthy pumpkin seedlings for mechanical inoculation. For insect transmission, five infective aphids were infected per seedling. Seedlings of eleven different species were inoculated mechanically for host range test. Clarified sap was examined under the electron microscope. Seeds of two pumpkin fruits from two different infected plants were planted and observed for disease transmission up to one-month old seedlings. The mosaic disease was transmitted mechanically from crude sap of different leaf samples to healthy pumpkin seedlings showing mosaic symptoms. The virus also infected eight cucurbits, i.e., cucumber (Cucumis sativus), green melon (Cucumis melo), orange/rock melon (C. melo), zucchini (Cucurbita pepo), pumpkin (Cucurbita maxima), water melon (Citrulus vulgaris), Bennicosa hispida, and blewah (Cucurbita sp.). Aphids  transmitted the disease from one to other pumpkin seedlings. The virus was not transmitted by seed. The mosaic disease of pumpkin at Maros, South Sulawesi, was associated with flexious particles of approximately 750 nm length, possibly a potyvirus, such as water melon mosaic virus rather than papaya ringspot virus or zucchini yellow mosaic virus.

scholarly journals Transgenic Resistance to Virus Diseases in Squash and Cantaloupe

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 766F-766
Author(s):  
George H. Clough ◽  
Philip B. Hamm
Keyword(s):  
Transgenic Plants ◽  
Mosaic Virus ◽  
Disease Incidence ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Field Virus ◽  
Elisa Testing ◽  
Transgenic Lines ◽  
High Virus

Three transgenic yellow crookneck squash (Cucurbita pepo var. melopepo) and five transgenic cantaloupe (Cucumis melo, Reticulatus group) lines were field-tested in 1993 and 1994, respectively, for resistance to Zucchini Yellow Mosaic Virus and Watermelon Mosaic Virus II. During both years, non-transgenic plants were inoculated with virus before transplanting to provide a high virus threat to the transgenic plants. Before and after transplanting, serological (ELISA) testing was used to obtain baseline information on transformed plants and to confirm field virus infection. In both years, plant disease development was rated weekly; yield was assessed during 1993. Disease progression, yield, and end-of-season ELISA indicated a significant reduction in frequency of disease incidence in the transgenic lines. Total squash yields did not differ between the transformed and unchanged lines, but the transgenic lines yielded more marketable fruit than the non-transgenic line.

scholarly journals First Report of Zucchini yellow mosaic virus, Watermelon mosaic virus, and Cucumber mosaic virus in Bottlegourd (Lagenaria siceraria) in Serbia

Plant Disease ◽  
2006 ◽  
Vol 90 (3) ◽  
pp. 380-380 ◽  
Author(s):  
N. Dukić ◽  
B. Krstić ◽  
I. Vico ◽  
J. Berenji ◽  
B. Duduk
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Mechanical Transmission ◽  
Lagenaria Siceraria ◽  
Indicator Plants ◽  
First Report ◽  
Das Elisa

During a cucurbit disease survey in August 2004, severe symptoms resembling those caused by viruses were observed on bottlegourd (Lagenaria siceraria (Molina) Standl.) in the Vojvodina region of Serbia. Symptoms included stunting, mosaic, green veinbanding, blistering, yellowing, chlorotic spots, leaf deformation, and fruit distortion. Leaf samples from 25 symptomatic plants were collected from two localities for virus identification using mechanical transmission and serological testing. Crude sap extract from leaf samples was mechanically inoculated onto bottlegourd and pumpkin (Cucurbita pepo) under greenhouse conditions. Field-collected bottlegourd and inoculated plants were tested using double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA). Positive reactions were obtained on collected and inoculated plants with polyclonal antiserum (Loewe Biochemica, Sauerlach, Germany) to Zucchini yellow mosaic virus(ZYMV) in 23 samples, with antiserum to Watermelon mosaic virus (WMV) in eight samples, and with antiserum to Cucumber mosaic virus (CMV) in seven samples. Each of the three viruses was detected in single as well as in mixed infections with the other two viruses. Biological characterization of viruses detected in single infections was done on the following indicator plants: Chenopodium amaranticolor, C. quinoa, Cucumis sativus, Cucumis melo, Citrullus lanatus, Nicotiana glutinosa, and N. tabacum cv. Samsun. The symptoms observed on indicator plants for each isolate corresponded to the results of DAS-ELISA (2,3). All three viruses are known to be important pathogens of cucurbit plants and were previously reported in pumpkin in Serbia (1). To our knowledge, this is the first report of ZYMV, WMV, and CMV in bottlegourd in Serbia. References: (1) N. Dukić et al. J. Agric. Sci. 47:149, 2002. (2) D. E. Lesemann et al. Phytopathol. Z. 108:304, 1983. (3) H. Rahimian and K. Izadpanah. Phytopathol. Z. 92:305, 1978.

scholarly journals First Report of Zucchini yellow mosaic virus in Cucumber in Poland

Plant Disease ◽  
2003 ◽  
Vol 87 (11) ◽  
pp. 1399-1399 ◽  
Author(s):  
H. Pospieszny ◽  
M. Cajza ◽  
R. Plewa
Keyword(s):  
Mosaic Virus ◽  
Cucurbita Pepo ◽  
Dna Analysis ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Chenopodium Quinoa ◽  
Yellow Mosaic Virus ◽  
Chenopodium Amaranticolor ◽  
Cycle Sequencing ◽  
First Report

In June 2002, mosaic and interveinal chlorosis were observed on two cucumber plants (Cucumis sativus) grown in one commercial greenhouse in the western region of Poland. Electron microscopic examination of leaf-dip preparations from infected plants showed flexuous filamentous virus particles typical of potyviruses (720 to 750 nm long). Chenopodium amaranticolor, Chenopodium quinoa, Citrullus lanatus, C. melo, C. sativus, Cucurbita maxima, Cucurbita pepo, Cucurbita pepo cv. Giromontiina, Cucurbita pepo cv. Patissoniana, Nicotiana benthamiana, and N. tabacum were mechanically inoculated with sap from symptomatic cucumber leaves. The virus caused local chlorotic lesions on Chenopodium amaranticolor and Chenopodium quinoa and systemic infection in all tested cucurbits but it did not infect tobacco plants. Reverse transcription-polymerase chain reaction (RT-PCR) amplification of the 3′ end of the genomic RNA was done by using P9502 as a downstream primer and degenerate CPUP as an upstream primer to amplify a highly conserved region of the potyviral coat protein (1). The PCR products were directly sequenced with the CEQ DTCS dye terminator cycle sequencing kit (Beckman Coulter, Inc., Fullerton, CA), and the analysis of dideoxy terminated fragments was conducted by capillary electrophoresis using a CEQ 2000 DNA Analysis System (Beckman Coulter, Inc.). The obtained 684 nt sequence (GenBank Accession No. AY347476) was almost identical with sequences of Zucchini yellow mosaic virus (ZYMV) isolates from Austria (GenBank Accession Nos. AJ420012-AJ420019 and AJ420027) and Hungary (GenBank Accession Nos. AJ459954 and AJ459955). The above suggested that the Polish isolate of ZYMV belonged to the Central European branch of the phylogenetic tree (2). To our knowledge, this is the first report of ZYMV in Poland. References: (1) R. A. A. van der Vlugt et al. Phytopathology 89:148, 1999. (2) I. Tobias and L. Palkovics. Pest Manage. Sci. 59:493, 2003.

scholarly journals High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 53
Author(s):  
Vivek Khanal ◽  
Harrington Wells ◽  
Akhtar Ali
Keyword(s):  
Phylogenetic Analysis ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
High Prevalence ◽  
The Us ◽  
Close Relationship ◽  
The Status ◽  
Low Incidence

Field information about viruses infecting crops is fundamental for understanding the severity of the effects they cause in plants. To determine the status of cucurbit viruses, surveys were conducted for three consecutive years (2016–2018) in different agricultural districts of Oklahoma. A total of 1331 leaf samples from >90 fields were randomly collected from both symptomatic and asymptomatic cucurbit plants across 11 counties. All samples were tested with the dot-immunobinding assay (DIBA) against the antisera of 10 known viruses. Samples infected with papaya ringspot virus (PRSV-W), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), and cucurbit aphid-borne-yellows virus (CABYV) were also tested by RT-PCR. Of the 10 viruses, PRSV-W was the most widespread, with an overall prevalence of 59.1%, present in all 11 counties, followed by ZYMV (27.6%), in 10 counties, and WMV (20.7%), in seven counties, while the remaining viruses were present sporadically with low incidence. Approximately 42% of the infected samples were positive, with more than one virus indicating a high proportion of mixed infections. CABYV was detected for the first time in Oklahoma, and the phylogenetic analysis of the first complete genome sequence of a CABYV isolate (BL-4) from the US showed a close relationship with Asian isolates.

scholarly journals Cucurbit aphid-borne yellows virus Is Prevalent in Field-Grown Cucurbit Crops of Southeastern Spain

Plant Disease ◽  
2007 ◽  
Vol 91 (3) ◽  
pp. 232-238 ◽  
Author(s):  
M. A. Kassem ◽  
R. N. Sempere ◽  
M. Juárez ◽  
M. A. Aranda ◽  
V. Truniger
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Rflp Analysis ◽  
Yellow Mosaic Virus ◽  
Multiple Infections ◽  
Watermelon Mosaic Virus ◽  
Limited Information ◽  
Growing Seasons ◽  
Beet Pseudo Yellows Virus ◽  
The Impact

Despite the importance of field-grown cucurbits in Spain, only limited information is available about the impact of disease on their production. During the 2003 and 2004 growing seasons, systematic surveys were carried out in open field melon (Cucumis melo) and squash (Cucurbita pepo) crops of Murcia Province (Spain). The fields were chosen with no previous information regarding their sanitation status, and samples were taken from plants showing viruslike symptoms. Samples were analyzed using molecular hybridization to detect Beet pseudo-yellows virus (BPYV), Cucurbit aphid-borne yellows virus (CABYV), Cucumber mosaic virus (CMV), Cucumber vein yellowing virus (CVYV), Cucurbit yellow stunting disorder virus (CYSDV), Melon necrotic spot virus (MNSV), Papaya ringspot virus (PRSV), Watermelon mosaic virus (WMV), and Zucchini yellow mosaic virus (ZYMV). We collected 924 samples from 48 field plots. Out of these, almost 90% were infected by at least one of the viruses considered, usually CABYV, which was present in 83 and 66% of the melon and squash samples, respectively. In the case of melon, CYSDV, BPYV, and WMV followed CABYV in relative importance, with frequencies of around 20 to 30%, while in squash, CVYV and BPYY showed frequencies between 28 and 21%. The number of multiple infections was very high, 66 and 56% of the infected samples of melon and squash, respectively, being afflicted. CABYV was present in all multiple infections. The high incidence of CABYV in single and multiple infections suggests that this virus may well become an important threat for cucurbit crops in the region. Restriction fragment length polymorphism (RFLP) analysis revealed that CABYV isolates can be grouped into two genetic types, both of which seemed to be present during the 2003 epidemic episode, but only one of the types was found in 2004.

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