scholarly journals Resistance Genes in the rp1 Region of Maize Effective Against Puccinia sorghi Virulent on the Rp1-D Gene in North America

Plant Disease ◽  
2001 ◽  
Vol 85 (2) ◽  
pp. 165-168 ◽  
Author(s):  
Jerald K. Pataky ◽  
Molly C. Pate ◽  
Scot H. Hulbert
Keyword(s):  
New York ◽  
North America ◽  
Resistance Genes ◽  
North American ◽  
Rust Resistance ◽  
Sweet Corn ◽  
Necrotic Tissue ◽  
Puccinia Sorghi ◽  
Rust Resistance Genes

Resistance in sweet corn conferred by the Rp1-D gene has controlled common rust, caused by Puccinia sorghi, in North American corn for nearly 15 years. Eleven isolates of P. sorghi virulent on corn with the Rp1-D gene were collected from Rp-resistant corn in 1999 from Wiscon-sin, Illinois, New York, and Minnesota. Isolates were increased on susceptible sweet corn. Urediniospores of nine isolates were bulked. Reactions of individual Rp genes in the rp1 region and reactions of linked combinations of Rp genes in the rp1 region (i.e., compound rust resistance genes) were evaluated against the bulked population of P. sorghi in several greenhouse trials. Reactions of individual and compound Rp genes also were evaluated against individual isolates of P. sorghi. Each trial contained at least two replicates of several lines with Rp genes and one susceptible check. Five to 10 two-leaved seedlings per line were inoculated at least twice with a suspension of urediniospores. Ten days after inoculation, rust reactions were rated:+ = sporulating uredinia, - = no sporulating uredinia, and I = chlorotic or necrotic tissue surrounding small uredinia. Four single genes, Rp1-E, Rp-G, Rp1-I, and Rp1-K, and eight compound genes, Rp1-JFC, Rp1-JC, Rp-GI, Rp-G5, Rp-GDJ, Rp-G5JD, Rp-G5JC, and Rp-GFJ, conferred resistance. Additional characterization of virulence in North American populations of P. sorghi that are avirulent against Rp1-D is necessary to determine if these genes will be as widely effective as the Rp1-D gene has been. Two subpopulations of P. sorghi were detected from the bulked population after it was sequentially cultured for at least five cycles on seedlings with Rp1-C or with Rp1-J. The subpopulation cultured on Rp1-J was avirulent on lines with Rp1-C/L/N, Rp1-B, and Rp1-M; whereas the subpopulation cultured on Rp1-C was virulent on lines with each of these genes. Both subpopulations were virulent on lines with Rp1-D.

scholarly journals Puccinia sorghi Virulent on Sweet Corn with the Rp1-D Gene in Southern France

Plant Disease ◽  
2001 ◽  
Vol 85 (5) ◽  
pp. 560-560
Author(s):  
J. K. Pataky ◽  
D. C. Plaisted ◽  
D. Scholten ◽  
H. F. de Durand
Keyword(s):  
North America ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Sweet Corn ◽  
Corn Hybrids ◽  
Puccinia Sorghi ◽  
First Time ◽  
Different Sources ◽  
Rust Resistance Genes

The Rp1-D gene, which conveys a chlorotic-fleck resistant reaction to Puccinia sorghi, effectively controlled common rust on sweet corn in North America for nearly 15 years. Biotypes of P. sorghi virulent on plants with the Rp1-D gene were widespread in North America for the first time in 1999 and again in 2000 (1,2). Many Rp-resistant sweet corn hybrids that are developed and grown in North America also are grown in Europe, including France where virulence against the Rp1-D gene has not been reported previously. In September 2000, uredinia of common rust were observed on and collected from sweet corn hybrids with the Rp1-D gene in commercial fields and hybrid trials in the Landes and Pyrénées Atlantiques departments of the Aquitaine region of southwestern France. Severity of rust generally was below 5% on these plants except for a few hybrids for which severity was about 20 to 30%. Common rust was not observed on hybrids with the Rp-G gene. Urediniospores were increased as a bulk population on the susceptible sweet corn hybrid Sterling in a greenhouse. Plants with each of 10 single Rp genes (Rp1-A, Rp1-C, Rp1-D, Rp1-E, Rp1-F, Rp1-I, Rp1-K, Rp1-L, Rp1-N, and Rp-G) or each of six compound rust resistance genes (Rp1-D5, Rp1-JC, Rp1-JFC, Rp-GDJ, Rp-GFJ, and Rp-G5JC) were assayed for reactions to this population of P. sorghi. Two to six different sources of seed of each single Rp gene and two different sources of seed of each compound rust resistance gene were replicates with a single pot of 6 to 18 plants grown from a specific seed source. Plants were inoculated three times on successive days by placing 2 or 3 ml of a urediniospore suspension in the whorl of two- to four-leaved seedlings. Reactions were rated 10 days after the last inoculation. Plants without symptoms or with chlorotic-fleck resistant reactions were inoculated again and rated 10 days later. Uredinia did not form on plants with compound rust resistance genes. Plants with the genes Rp1-E, Rp1-I, Rp1-K, and Rp-G also were resistant although a few, very small uredinia (i.e., type-1 uredinia) were observed on a few plants. Plants with the genes Rp1-A, Rp1-C, Rp1-D, Rp1-F, Rp1-L, and Rp1-N were fully susceptible. This pattern of virulence is the same as that observed during the past two years in North American populations of P. sorghi virulent against Rp1-D. Rp-resistance currently available in most sweet corn hybrids will not be effective in France if these virulent biotypes become prevalent. References: (1) J. K. Pataky et al. Plant Dis. 85:165, 2001. (2) M. C. Pate et al. Plant Dis. 84:1154, 2000.

scholarly journals Puccinia sorghi in Sinaloa, Mexico Virulent on Corn with the Rp1-D Gene

Plant Disease ◽  
2000 ◽  
Vol 84 (7) ◽  
pp. 810-810 ◽  
Author(s):  
J. K. Pataky ◽  
T. A. Natti ◽  
E. B. Snyder ◽  
C. J. Kurowski
Keyword(s):  
United States ◽  
New York ◽  
North America ◽  
Rust Resistance ◽  
Sweet Corn ◽  
The United States ◽  
Inbred Lines ◽  
Corn Hybrids ◽  
Midwestern United States ◽  
Puccinia Sorghi

Several different Rp genes in corn condition chlorotic fleck resistant reactions to Puccinia sorghi. Rp-resistance has been used successfully for the past 15 years to control common rust on sweet corn in North America. Most, but not all, Rp-resistant sweet corn hybrids carry the Rp1-D gene. In August and September 1999, isolates of P. sorghi were collected from Rp-resistant sweet corn grown in Illinois, Wisconsin, Minnesota, Michigan, and New York. This was the first widespread occurrence in North America of P. sorghi virulent on corn with Rp1-D (2). The origin of this population of P. sorghi with a virulence phenotype new to North America is not known. Since many believe Mexico is the source of common rust inocula for the midwestern United States, it is important to discover if this virulence occurs in Mexico. Forty-one Rp-resistant and nine susceptible sweet corn hybrids were planted 8 December 1999 in a nursery near Los Mochis, Mexico, in the state of Sinalao. Rp-resistant hybrids had been effective against common rust in Los Mochis nurseries prior to 1999. Each hybrid was in a single row of about 30 plants. The Los Mochis nursery also included two replicate rows of sweet corn or field corn inbred lines with one of 17 different single Rp-genes or one of 11 different compound genes for rust resistance (1). Plants were exposed to local populations of P. sorghi. Reactions were rated in March 2000. Sporulating uredinia (susceptible reactions) were abundant on all sweet corn hybrids and on inbreds with Rp1-D. Susceptible reactions also were observed on other inbred lines with Rp-genes except for lines with the single genes: Rp1-E, Rp-G, Rp1-I, Rp1-K, and Rp1-L or lines with the compound rust genes: Rp1-GI, Rp1-G5, Rp1-GDJ, Rp1-GFJ, Rp1-G5JC, Rp1-G5JD, and Rp1-JFC. This pattern of virulence is similar to that of P. sorghi isolates collected in the midwestern United States in 1999. Rp-resistance currently available in most sweet corn hybrids grown in the Midwest will not be effective when this population of P. sorghi spreads from Mexico to the United States. Therefore, other sources of rust resistance need to be incorporated into sweet corn hybrids. References: (1) S. H. Hulbert, Annu. Rev. Phytopathol. 35:292, 1997. (2) J. K. Pataky and W. F. Tracy. Plant Dis. 83:1177, 1999.

Characterization of Wheat Stripe Rust Resistance Genes in Shaanmai 139

2010 ◽  
Vol 36 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Hong ZHANG ◽  
Zhi-Long REN ◽  
Yin-Gang HU ◽  
Chang-You WANG ◽  
Wan-Quan JI
Keyword(s):  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Stripe Rust Resistance ◽  
Wheat Stripe Rust ◽  
Rust Resistance Genes

scholarly journals Structural and functional analysis of genes with potential involvement in resistance to coffee leaf rust: a functional marker based approach

2019 ◽  
Author(s):  
Geleta Dugassa Barka ◽  
Eveline Teixeira Caixeta ◽  
Sávio Siqueira Ferreira ◽  
Laércio Zambolim
Keyword(s):  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Function Analysis ◽  
Bac Library ◽  
Leaf Rust Resistance ◽  
Molecular Data ◽  
Coffee Leaf Rust ◽  
Rust Resistance Genes

AbstractPhysiology-based differentiation of SH genes and Hemileia vastatrix races is the principal method employed for the characterization of coffee leaf rust resistance. Based on the gene-for-gene theory, nine major rust resistance genes (SH1-9) have been proposed. However, these genes have not been characterized at the molecular level. Consequently, the lack of molecular data regarding rust resistance genes or candidates is a major bottleneck in coffee breeding. To address this issue, we screened a BAC library with resistance gene analogs (RGAs), identified RGAs, characterized and explored for any SH related candidate genes. Herein, we report the identification and characterization of a gene (gene 11), which shares conserved sequences with other SH genes and displays a characteristic polymorphic allele conferring different resistance phenotypes. Furthermore, comparative analysis of the two RGAs belonging to CC-NBS-LRR revealed more intense diversifying selection in tomato and grape genomes than in coffee. For the first time, the present study has unveiled novel insights into the molecular nature of the SH genes, thereby opening new avenues for coffee rust resistance molecular breeding. The characterized candidate RGA is of particular importance for further biological function analysis in coffee.

scholarly journals Mapping and characterization of wheat stem rust resistance genes SrTm5 and Sr60 from Triticum monococcum

2017 ◽  
Vol 131 (3) ◽  
pp. 625-635 ◽  
Author(s):  
Shisheng Chen ◽  
Yan Guo ◽  
Jordan Briggs ◽  
Felix Dubach ◽  
Shiaoman Chao ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Stem Rust Resistance ◽  
Triticum Monococcum ◽  
Wheat Stem Rust ◽  
Stem Rust Resistance Genes ◽  
Rust Resistance Genes

scholarly journals Discovery and characterization of two new stem rust resistance genes in Aegilops sharonensis

2017 ◽  
Vol 130 (6) ◽  
pp. 1207-1222 ◽  
Author(s):  
Guotai Yu ◽  
Nicolas Champouret ◽  
Burkhard Steuernagel ◽  
Pablo D. Olivera ◽  
Jamie Simmons ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Stem Rust Resistance ◽  
Aegilops Sharonensis ◽  
Stem Rust Resistance Genes ◽  
Rust Resistance Genes

scholarly journals Breeding of common wheat for leaf rust resistance using DNA markers

2020 ◽  
Author(s):  
E.R. Davoyan ◽  
◽  
L.A. Bespalova ◽  
R.O. Davoyan ◽  
E.V. Agaeva ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Common Wheat ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Selected Lines ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

The article presents the results of the characterization of 277 lines of common wheat developed in the National Center of Grain named after P.P. Lukyanenko by the presence of molecular markers linked to leaf rust resistance genes Lr9, Lr19, Lr24, Lr37, Lr26. Lines with Lr9 and Lr19 were not identified. We detected 52 lines carrying Lr24; 80 lines with Lr26; 141 lines with Lr37. Lines carrying a combination of leaf rust resistance genes were selected using molecular markers. The presence of a combination of Lr37 + Lr26 was established in 31 lines. The combination of Lr24 + Lr26 was detected in 12 lines. Line 125-15 Ms 2 carries a combination of Lr37 + Lr24. A pyramid of three genes was found in the line 144-15 Ms 2. Currently, the selected lines are widely involved in the breeding process.

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