scholarly journals Biological Control of Sclerotinia Stem Rot of Soybean with Sporidesmium sclerotivorum

Plant Disease ◽  
2002 ◽  
Vol 86 (9) ◽  
pp. 999-1004 ◽  
Author(s):  
L. E. del Rio ◽  
C. A. Martinson ◽  
X. B. Yang
Keyword(s):  
Biological Control ◽  
Block Design ◽  
Field Studies ◽  
Control Agent ◽  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
Field Crops ◽  
Randomized Complete Block Design ◽  
Experimental Plots ◽  
Commercial Field

Field studies were conducted to evaluate the effectiveness of Sporidesmium sclerotivorum to control Sclerotinia stem rot of soybean (SSR) at Ames, Humboldt, and Kanawha, IA, between 1996 and 1998. Experimental plots (3 × 3 m) were infested with S. sclerotivorum macroconidia once at a rate of 0, 2, or 20 spores per cm2 in the fall of 1995 or the spring of 1996, under two crop rotation schemes. A randomized complete block design with four replications in each location was used. Plots infested with 20 spores per cm2 had 62% less SSR (P = 0.05) than control plots at Humboldt in 1996. No differences were detected between fall and spring applications. In 1998, plots treated with either 2 or 20 spores per cm2 had 51 to 63% less SSR (P = 0.05) than control plots at Ames and Kanawha. In 1998, SSR was completely suppressed in all plots at Humboldt, while the commercial field surrounding the experimental plots had 17% SSR. S. sclerotivorum was retrieved from all infested plots at all locations 2 years after infestation with sclerotia of Sclerotinia sclerotiorum as bait. At Humboldt, S. sclerotivorum was also retrieved from control plots. Two larger plots (10 × 10 m) were infested with 20 or 100 spores per cm2 in the fall of 1996 or spring of 1997 in six commercial fields. SSR incidence, which was measured in transects up to 20 m from the infested area at 5-m intervals, was reduced 56 to 100% (P = 0.05) in four fields compared with the surrounding uninfested areas in the commercial fields. Dispersal of the control agent was evident by the fact that SSR incidence gradually increased from the edge of the infested macroplots up to about 10 m into noninoculated areas of the commercial field. This paper constitutes the first report describing the biocontrol of a disease on field crops that may be employed economically.

scholarly journals Existence of entomopathogen fungi, Beauveria bassiana as an endophyte in cacao seedlings

2015 ◽  
Vol 31 (3) ◽  
pp. 186-195
Author(s):  
Endang Sulistyowati ◽  
Febrilia Nur AINI
Keyword(s):  
Beauveria Bassiana ◽  
Plant Protection ◽  
Biological Control Agent ◽  
Block Design ◽  
Control Agent ◽  
Post Inoculation ◽  
Pod Borer ◽  
Fungal Entomopathogens ◽  
Randomized Complete Block Design ◽  
Fungal Entomopathogen

Beauveria bassiana is one of the entomopathogen fungi which is known as biological control agent of cocoa pod borer and cocoa mirids (Helopeltis spp.). Because of its effectiveness in the fields is still not consistent, so we conduct a research with the objective to know the possibility of Beauveria bassiana to be established as a endophyte. Various fungal entomopathogens have already been reported as endophytes and the various methods used to inoculate the plants with B. bassiana were partially effective. The research has been conducted in laboratory of Plant Protection, Indonesian Coffee and Cocoa Research Institute by inoculating of cocoa seeds and cocoa nursery with B. bassiana suspension.  The trial was arranged  by randomized complete block design with a factorial arrangement. The factor were spore concentration of B. bassiana (0; 2; and 4 g/ 10 l) and cocoa varieties (family of ICS 60, TSH858, and hybrid). The trial were use  four replications. The results showed that the fungal entomopathogen B. bassiana was established as an endophyte in cocoa seedling, both from cocoa seeds and nursery application. Percentage of existence of B. bassiana colonies as endophytes one month after seeds application were ICS 60 amounted to 93.3 % both on concentration treatments, while the families of TSH 858 by 80 % and 86.67 % respectively in 2 g and 4 g per 10 l of B. bassiana spores concentration treament.. The lowest percentage was in hybrids, which amounted to 66.67% and 50%. B. bassiana colonies was exixtence as an endophyte in culture from root, stem and leaves of cocoa seedling up to 5 months post inoculation. While the application on nursery by soil drenshing, leaf spraying, and stem injection , it was known that B. bassiana colonies were found in the tissues of leaves, stems, and roots until two months after application. Colonies of B. bassiana as endophytes still exsist until six weeks after nursery was planted in the field. 

scholarly journals Evaluation of experimental designs in durum wheat trials

2015 ◽  
Vol 52 (2) ◽  
pp. 105-114
Author(s):  
Anastasios Katsileros ◽  
Christos Koukouvinos
Keyword(s):  
Durum Wheat ◽  
Block Design ◽  
Field Trials ◽  
Experimental Designs ◽  
Incomplete Block ◽  
Wheat Genotypes ◽  
Randomized Complete Block Design ◽  
Experimental Plots

Abstract Variability among experimental plots may be a relevant problem in field genotype experiments, especially when a large number of entries are involved. Four field trials on 24 durum wheat genotypes were conducted in 2013/14 in order to evaluate the efficiency of Incomplete Block, Alpha and Augmented designs in comparison with the traditional Randomized Complete Block Design (RCBD). The results showed that the RCBD can be replaced by an Alpha design, which provides better control of variability among the experimental units when the number of treatments to be tested in an experiment exceeds twenty. The ranking of the genotypes across the four designs was not constant.

scholarly journals Potency of plant-derived biological nematicides on root-knot disease of tomato, Lycopersicon lycopersicum (L.) Mill

2021 ◽  
Vol 7 (1) ◽  
pp. 1-5
Author(s):  
Olabiyi T.I
Keyword(s):  
Block Design ◽  
Water Extract ◽  
Growth And Yield ◽  
Root Knot Nematode ◽  
Tomato Root ◽  
Tomato Production ◽  
University Of Technology ◽  
Randomized Complete Block Design ◽  
Lycopersicon Lycopersicum ◽  
Experimental Plots

The aim of this research was to evaluate the effectiveness of plant-derived biological nematicides in the management of root-knot nematode disease of tomato. A piece of land measuring 8 m × 54 m was ploughed and raised beds were the experimental plots. The land was divided into 4 blocks, each block comprising 12 treatments and 4 replicates with a total of 48 experimental plots were fitted into randomized complete block design (RCBD). The experiment was carried out at the Teaching and Research Farms, Faculty of Agricultural Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria (Longitude 40E and Latitude 8010N) during 2018 planting season. 20 g macerated root gall of Celosia was applied to tomato root rhizosphere (inoculation). The plant-derived biological nematicides that were assessed on root-knot nematode disease of tomato, UC8 cultivar, include the leaf water extract of neem, castor, wild sunflower, cleome, siam weed, phyllanthus, nitta, tobacco, lantana and calotropis, mixed with black soap, at rate of 9:1 v/w. The black soap and distilled water served as adjuvant and solvent respectively, and as the control experiments. The results showed that the root galling and soil population of root-knot nematode were significantly reduced with consequent enhanced growth and yield of tomato in the plots treated with plant-derived biological nematicides, compared with the tomato in the control experiments. The study has demonstrated that the use of plant-derived biological nematicides can be effectively explored in the management of root-knot nematode disease. The application of plant-derived biological nematicides is therefore recommended for sustainable management of root-knot nematode disease in tomato production.

scholarly journals Effect of Auxin Spraying and Plant Extracts on Two Cultivars of Bean (Vicia Faba L.)

2021 ◽  
Vol 923 (1) ◽  
pp. 012009
Author(s):  
Jaber Jassim Abotlasha ◽  
Falah Hasan Issa ◽  
Fouad Razzaq Al-Burki
Keyword(s):  
Plant Height ◽  
Vicia Faba ◽  
Plant Extracts ◽  
Block Design ◽  
Cropping Season ◽  
Randomized Complete Block Design ◽  
Vicia Faba L ◽  
Experimental Plots

Abstract The field-based experiment was conducted during the 2020 – 2021 cropping season to evaluate the response of two types of bean (Vicia faba L.): Luz De Otono (C1) and Aquadulce (C2 for different spray treatments namely, control (T1), 40mg IAA.L-1 (T2) and 10gm of licorice with yeast.L-1 (T3),. The treatments were assigned to experimental plots following a randomized complete block design (R.C.B.D). Each treatment replicated trice time. Means were compared based on least significant differences (LSD) at 0.05 of probability. Plant height, leaves area, chlorophyll, N and K content in leaves were recorded as parameters. Results showed that the Aquadulce variety significantly increased in most studied measurements. T3 was significant and superior compare to other treatments in plant height (68.28cm), leaves the area (51.5dcm2), chlorophyll (23.98 SPAD), N(4.623%), and K(3.120%). Results also indicated that the interaction of C2T3 had a significant increase in plant height(68.95cm), leaves the area (57.0 dcm2), chlorophyll, N(4.88%), and K(2.68%).

Biological Control of Sclerotinia Stem Rot (S. minor ) of Sunflower Using Trichoderma Species

2006 ◽  
Vol 5 (2) ◽  
pp. 228-232 ◽  
Author(s):  
J. Abdollahza ◽  
E. Mohammadi Goltape . ◽  
H. Rouhani .
Keyword(s):  
Biological Control ◽  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
Trichoderma Species

PERTUMBUHAN DAN HASIL BLEWAH (Cucumis melo var. cantalupensis L.) PADA TINGKAT PENJARANGAN BUAH DAN DOSIS PUPUK NPK BERBEDA

2021 ◽  
Vol 23 (2) ◽  
pp. 72-77
Author(s):  
Lusiana Septiriyani ◽  
Mohammad Chozin ◽  
Yulian Yulian
Keyword(s):  
Fruit Quality ◽  
Cucumis Melo ◽  
Block Design ◽  
Crop Productivity ◽  
Fertilizer Application ◽  
Fruit Thinning ◽  
Randomized Complete Block Design ◽  
Npk Fertilizer ◽  
Experimental Plots

[PRODUCTIVITY AND FRUIT QUALITY OF CANTALOUPE AT DIFFERENT RATES OF FRUIT THINNING AND NPK FERTILIZER APPLICATION]. Productivity and fruit quality of cantaloupe depend on the availability of nutrients and the number of fruits maintained. The aim of this study was to compare the productivity and fruit quality of cantaloupe at different rates of fruit thinning and NPK fertilizer application. The research was conducted in August-October 2020 on street Kapuas V, Lingkar Barat Village, Gading Cempaka District, Bengkulu city. A field experiment was arranged in a randomized complete block design (RCBD) with 3 replications for alloting two factorial treatments structure, namely rates of fruit thinning (no fruit thinning, thinned to one fruit /plant, thinned to two fruits/plant, and thinned to three fruits/plant) and rates of NPK fertilizer application (400, 600, and 800 kg/ha) on rhe experimental plots. The results showed that fruit thinnings resulted no increament in crop productivity, but the fruit quality was improved as fewer number of fruits were retained on each plant. The NPK fertilizer at 400, 600, and 800 kg/ha were not able to increase the productivity of cantaloupe  and the quality of the  produced fruit when  the application were made only at planting and 23 days after planting. 

STUDIES ON FIELD EVALUATION OF BRASSICA GERMPLASM/VARIETIES AGAINST SCLEROTINIA SCLEROTIORUM UNDER INOCULATED CONDITION

2017 ◽  
Vol 23 (1) ◽  
Author(s):  
PRAMOD KUMAR FATEHPURIA ◽  
RAJNI S. SASODE ◽  
DEVASHISH R. CHOBE ◽  
VEERENDRA GUPTA ◽  
REETI SINGH
Keyword(s):  
Plant Pathology ◽  
Sclerotinia Sclerotiorum ◽  
Block Design ◽  
Indian Mustard ◽  
Field Evaluation ◽  
Stem Rot ◽  
Sclerotinia Stem Rot ◽  
College Of Agriculture ◽  
Centre Of Origin ◽  
Sclerotinia Blight

Indian mustard, Brassica juncea belong to Brassicaceae and Centre of origin Mideterian. Sclerotinia stem rot/ blight caused by Sclerotinia sclerotiorum is one of the most important fungal diseases in rapeseed and other crops in the world. The present study was undertaken to access the forty seven germplasm/varieties to find out the resistance against S. sclerotiorum under inoculated condition. The reaction of forty-seven germplasm was evaluated in randomized complete block design with thrice replications at the experimental field, Department of Plant Pathology, College of Agriculture, Gwalior during Rabi season 2014-15 and laboratory work was done in the Department of Plant Pathology, College of Agriculture, Gwalior (M.P.). Observations for severity of stem rot on main stem were recorded after 120 days of sowing using 0 - 4 scale. (Assessment scale 0-4 where 0 = no disease (resistance), 1 = less than half stem girdled (moderate resistance), 2 = more than half stem girdled (susceptible), 3 = whole stem girdled (moderate susceptible), 4 = plant dead (highly suscepitble). Forty-seven germplasm/varieties were evaluated for their reaction against Sclerotinia blight (Sclerotinia sclerotiorum). Out of forty seven germplasm / varieties, tested two Cultivar SBG-14-5 and SBG-14- 6 gave highly resistance reactions, while twenty six varieties are found resistant SBG-14-2, SBG-14-3, SBG-14-4, SBG-14-7, SBG-14-8, SBG-14-9, SBG-14-10, SBG-14-11, SBG-14-13, SBG14-15, SBG-14-17, SBG-14-18, SBG-14-23, SBG-14-27, SBG-14-28, SBG-14-30, SBG-14-31, SBG-14-32, SBG-14-33, SBG-14-34, SBG-14-35, SBG-14-36, SBG- 14-41, SBG-14-42, NDNSR-14-76, NDNSR-14-77, twelve varieties are found susceptible SBG-14-1, SBG-14-12, SBG- 14-14, SBG-14-16, SBG-14-21, SBG-14-22, SBG-14-24, SBG-14-26, SBG-14-29, SBG-14-37, SBG-14-39, SBG-14-40 and seven varieties SBG-14-19, SBG-14-20, SBG-14-25, SBG-14-38, Varuna, Rohini and BIOYSR were found highly susceptible against Sclerotinia blight (Sclerotinia sclerotiorum).

scholarly journals MAIZE–SOYBEAN INTERCROPPING FOR SUSTAINABLE INTENSIFICATION OF CEREAL–LEGUME CROPPING SYSTEMS IN NORTHERN NIGERIA

2017 ◽  
Vol 55 (1) ◽  
pp. 73-87 ◽  
Author(s):  
ALPHA Y. KAMARA ◽  
ABDULLAHI I. TOFA ◽  
TEMITOPE ADEMULEGUN ◽  
REUBEN SOLOMON ◽  
HAULATU SHEHU ◽  
...  
Keyword(s):  
Cropping Systems ◽  
Block Design ◽  
Field Studies ◽  
Northern Nigeria ◽  
Land Equivalent Ratio ◽  
Northern Guinea Savanna ◽  
Monetary Value ◽  
Guinea Savanna ◽  
Randomized Complete Block Design ◽  
Sole Cropping

SUMMARYField studies were conducted during the 2014 and 2015 wet seasons at Zaria in the northern Guinea savanna and at Iburu in the southern Guinea savanna of Nigeria to determine the productivity of maize–soybean intercropping system. There were four treatment combinations in the experiment: sole maize; sole soybean; maize spaced at 50 cm and intercropped with soybean; and maize spaced at 65 cm and intercropped with soybean. The experiment was laid out in a randomized complete block design with three replications. The results showed that sole cropped maize and soybean generally outperformed the intercropped component crops. Land Equivalent Ratio (LER) was greater than 1 for all the intercrop treatments, indicating that it is advantageous to grow maize and soybean in association than in pure stands. Except for 2014 in Zaria, LER for intercropped maize spaced at 50 cm was higher than that for maize spaced at 65 cm. Gross Monetary Value (GMV) was generally higher for intercrops than sole crops except in Iburu in 2015 where GMV for intercropped maize spaced at 65 cm was similar to those of sole maize and soybean. Monetary Advantage Index (MAI) was positive for all intercrop treatments in both locations and years, which shows definite yield and economic advantages compared to the sole cropping systems. This suggests that farmers can intercrop soybean and maize with maize spaced at 50 cm and 65 cm.

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