The Residual and Interactive Expressions of 'Defeated' Wheat Stem Rust Resistance Genes

Wheat stem rust caused by Puccinia graminis f. sp. tritici Eriks. & E. Henn. (Pgt), is a major disease that has been effectively controlled using resistance genes. The appearance and spread of Pgt races such as Ug99, TKTTF, and TTTTF, which are virulent to most stem rust-resistant genes currently deployed in wheat breeding programs, renewed the interest in breeding cultivars resistant to wheat stem rust. It is therefore important to investigate the levels of resistance or vulnerability of wheat cultivars to Pgt races. Resistance to Pgt races 21C3CTHQM, 34MKGQM, and 34C3RTGQM was evaluated in 136 Chinese wheat cultivars at the seedling stage. A total of 124 cultivars (91.2%) were resistant to the three races. Resistance genes Sr2, Sr24, Sr25, Sr26, Sr31, and Sr38 were analyzed using molecular markers closely linked to them, and 63 of the 136 wheat cultivars carried at least one of these genes: 21, 25, and 28 wheat cultivars likely carried Sr2, Sr31, and Sr38, respectively. Cultivars “Kehan 3” and “Jimai 22” likely carried Sr25. None of the cultivars carried Sr24 or Sr26. These cultivars with known stem rust resistance genes provide valuable genetic material for breeding resistant wheat cultivars.

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Evaluation and identification of stem rust resistance genesSr2,Sr24,Sr25,Sr26,Sr31andSr38in wheat lines from Gansu Province in China

PeerJ ◽

10.7717/peerj.4146 ◽

2017 ◽

Vol 5 ◽

pp. e4146 ◽

Cited By ~ 3

Author(s):

Xiao Feng Xu ◽

Dan Dan Li ◽

Yang Liu ◽

Yue Gao ◽

Zi Yuan Wang ◽

...

Keyword(s):

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Gansu Province ◽

Wheat Cultivars ◽

Wheat Stem Rust ◽

Stem Rust Resistance Genes ◽

Wheat Lines ◽

Rust Resistance Genes

Wheat stem rust, caused byPuccinia granimisf. sp.tritici, severely affects wheat production, but it has been effectively controlled in China since the 1970s. However, the appearance and spread of wheat stem rust races Ug99 (TTKSK, virulence toSr31), TKTTF (virulence toSrTmp) and TTTTF (virulence to the cultivars carryingSr9eandSr13) have received attention. It is important to clarify the effectiveness of resistance genes in a timely manner, especially for the purpose of using new resistance genes in wheat cultivars for durable-resistance. However, little is known about the stem rust resistance genes present in widely used wheat cultivars from Gansu. This study aimed to determine the resistance level at the seedling stage of the main wheat cultivars in Gansu Province. A secondary objective was to assess the prevalence ofSr2,Sr24,Sr25,Sr26,Sr31, andSr38using molecular markers. The results of the present study indicated that 38 (50.7%) wheat varieties displayed resistance to all the tested races ofPuccinia graminisf. sp.tritici.The molecular marker analysis showed that 13 out of 75 major wheat cultivars likely carriedSr2; 25 wheat cultivars likely carriedSr31; and nine wheat cultivars likely carriedSr38. No cultivar was found to haveSr25andSr26, as expected. Surprisingly, no wheat cultivars carriedSr24. The wheat lines with known stem rust resistance genes could be used as donor parent for further breeding programs.

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Correction to: Mapping and characterization of wheat stem rust resistance genes SrTm5 and Sr60 from Triticum monococcum

Theoretical and Applied Genetics ◽

10.1007/s00122-018-3194-3 ◽

2018 ◽

Vol 131 (11) ◽

pp. 2493-2493

Author(s):

Shisheng Chen ◽

Yan Guo ◽

Jordan Briggs ◽

Felix Dubach ◽

Shiaoman Chao ◽

...

Keyword(s):

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Triticum Monococcum ◽

Wheat Stem Rust ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes

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Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm

Theoretical and Applied Genetics ◽

10.1007/s00122-005-2039-z ◽

2005 ◽

Vol 111 (3) ◽

pp. 496-504 ◽

Cited By ~ 116

Author(s):

R. Mago ◽

H. S. Bariana ◽

I. S. Dundas ◽

W. Spielmeyer ◽

G. J. Lawrence ◽

...

Keyword(s):

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Pcr Markers ◽

Wheat Stem Rust ◽

Wheat Germplasm ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes ◽

Selection Of

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Races of Puccinia graminis Identified in the United States During 2003

Plant Disease ◽

10.1094/pd-89-1125 ◽

2005 ◽

Vol 89 (10) ◽

pp. 1125-1127 ◽

Cited By ~ 28

Author(s):

Y. Jin

Keyword(s):

United States ◽

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

The United States ◽

Stem Rust Resistance ◽

Puccinia Graminis ◽

Wheat Stem Rust ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes

Stem rust of small grain cereals, caused by Puccinia graminis, is a major disease of wheat, barley, and oat. In order to effectively utilize stem rust resistance in the improvement of small grain cereals, it is necessary to monitor the virulence composition and dynamics in the stem rust population. Races of P. graminis from barberry, wheat, barley, and oat were surveyed across the United States during 2003. Aecial infections on barberry were primarily due to P. graminis f. sp. secalis, as inoculations using aeciospores failed to produce infection on wheat and oat. Race QFCS of P. graminis f. sp. tritici was the most common race identified from wheat and barley. Race QFCS has virulence on stem rust resistance genes Sr5, 8a, 9a, 9d, 9g, 10, 17, and 21 that are used for race identification. Race TTTT was identified in 2003. This race possesses virulence to all 16 stem rust resistance genes present in the wheat stem rust differentials and should be targeted in breeding for stem rust resistance. Race QFCN appeared to be a new race in the U.S. stem rust population. Races QCCJ and MCCF were identified, but at low frequencies. Seven races of P. graminis f. sp. avenae were identified from oat, and races NA-27, NA-29, and NA-67 were the predominant races. Race NA-76 was identified for the first time in the United States.

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Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26

Theoretical and Applied Genetics ◽

10.1007/s00122-009-1186-z ◽

2009 ◽

Vol 120 (4) ◽

pp. 691-697 ◽

Cited By ~ 50

Author(s):

Sixin Liu ◽

Long-Xi Yu ◽

Ravi P. Singh ◽

Yue Jin ◽

Mark E. Sorrells ◽

...

Keyword(s):

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Pcr Markers ◽

Wheat Stem Rust ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes

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Genetic Stability of Wheat Stem Rust Resistance Genes under Egyptian Field Conditions

Egyptian Journal of Phytopathology ◽

10.21608/ejp.2018.87793 ◽

2018 ◽

Vol 46 (1) ◽

pp. 215-233

Author(s):

Mohamed Abou-Zeid ◽

Walid El-Orabey ◽

Reda Omara ◽

Ahmed Mohamed ◽

Mamdouh Ashmawy

Keyword(s):

Resistance Genes ◽

Stem Rust ◽

Genetic Stability ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Field Conditions ◽

Wheat Stem Rust ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes

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Seedling Stage Resistance of Iranian Bread Wheat Germplasm to Race Ug99 of Puccinia graminis f. sp. tritici

Plant Disease ◽

10.1094/pdis-02-12-0138-re ◽

2013 ◽

Vol 97 (3) ◽

pp. 387-392 ◽

Cited By ~ 2

Author(s):

Mohsen Mohammadi ◽

Davoud Torkamaneh ◽

Mehran Patpour

Keyword(s):

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Stem Rust Resistance ◽

Puccinia Graminis ◽

Wheat Cultivars ◽

Breeding Lines ◽

Wheat Germplasm ◽

Stem Rust Resistance Genes ◽

Rust Resistance Genes

Following emergence of Ug99, the new virulent race of Puccinia graminis f. sp. tritici in Africa, a global effort for identification and utilization of new sources of Ug99-resistant germplasm has been undertaken. In this study, we conducted replicated experiments to evaluate the resistance of Iranian wheat germplasm to the TTKSK lineage of the Ug99 race of P. graminis f. sp. tritici. We also evaluated for presence of stem rust resistance genes (i.e., Sr2, Sr24, Sr26, Sr38, Sr39, Sr31, and Sr1RSAmigo) in wheat cultivars and breeding lines widely cultivated in Iran. Our phenotyping data revealed high levels of susceptibility to Ug99 in Iranian bread wheat germplasm. Our genotyping data revealed that Iranian cultivars do not carry Sr24, Sr26, or Sr1RSAmigo. Only a few salt-tolerant cultivars and breeding lines tested positively for Sr2, Sr31, Sr38, or Sr39 markers. In conclusion, the genetic basis for resistance to Ug99 in Iranian wheat cultivars was found to be vulnerable. Acquiring knowledge about existing resistance genes and haplotypes in wheat cultivars and breeding lines will help breeders, cereal pathologists, and policy makers to select and pyramid effective stem rust resistance genes.

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Stem Rust Resistance in A-Genome Diploid Relatives of Wheat

Plant Disease ◽

10.1094/pdis-04-10-0260 ◽

2011 ◽

Vol 95 (8) ◽

pp. 941-944 ◽

Cited By ~ 39

Author(s):

M. N. Rouse ◽

Y. Jin

Keyword(s):

Resistance Genes ◽

Stem Rust ◽

Rust Resistance ◽

Infection Type ◽

Genetic Resistance ◽

Wheat Breeding ◽

Stem Rust Resistance ◽

Wheat Stem Rust ◽

A Genome ◽

Stem Rust Resistance Genes

Wheat stem rust, caused by Puccinia graminis f. sp. tritici, has been effectively controlled through the use of genetic resistance. P. graminis f. sp. tritici race TTKSK (Ug99) possesses virulence to many resistance genes that have been used in wheat breeding worldwide. One strategy to aid breeders in developing resistant cultivars is to utilize resistance genes transferred from wild relatives to wheat. Stem rust resistance genes have previously been introgressed from Triticum monococcum to wheat. In order to identify additional resistance genes, we screened 1,061 accessions of T. monococcum and 205 accessions of T. urartu against race TTKSK and four additional P. graminis f. sp. tritici races: TTTTF, TRTTF, QFCSC, and MCCFC. A high frequency of the accessions (78.7% of T. monococcum and 93.0% of T. urartu) were resistant to P. graminis f. sp. tritici race TTKSK, with infection types ranging from 0 to 2+. Among these resistant accessions, 55 T. monococcum accessions (6.4% of the total) were also resistant to the other four races. Associations of resistance in T. monococcum germplasm to different races indicated the presence of genes conferring resistance to multiple races. Comparing the observed infection type patterns to the expected patterns of known genes indicated that previously uncharacterized genes for resistance to race TTKSK exist in both T. monococcum and T. urartu.

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