Rapid Magnetic Microsphere Enzyme Immunoassay for Potato Virus X and Potato Leafroll Virus

Although all three viruses are commonly found in potatoes throughout the world, this is the first report of potato viruses from Alaska to be sequenced and molecularly analyzed for comparisons with known viruses. Accepted for publication 17 January 2011. Published 9 February 2011.

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Resistance to potato leafroll virus infection and accumulation in potato cultivars, and the effects of previous infection with other viruses on expression of resistance

Australian Journal of Agricultural Research ◽

10.1071/ar9931891 ◽

1993 ◽

Vol 44 (8) ◽

pp. 1891 ◽

Cited By ~ 24

Author(s):

CR Wilson ◽

RAC Jones

Keyword(s):

Potato Virus ◽

Potato Virus X ◽

Potato Leafroll Virus ◽

Potato Cultivars ◽

Infected Leaf ◽

Potato Virus S ◽

Breeding Lines ◽

Infection Pressure ◽

Previous Infection ◽

Leafroll Virus

A selection of potato cultivars and breeding lines was evaluated for presence of resistance to infection with potato leafroll virus (PLRV) via viruliferous aphid vectors ( IR) and/or resistance to accumulation of PLRV antigen ( AR) in infected leaf tissue. Cultivars Aracy, Delcora, Omega and Spunta, and breeding lines BR63.15 and B71.240.2 carried both IR and AR , Bismark, Serrana INTA and L/T1 had alone and Delaware had AR alone. The other cultivars tested had neither. Within both the resistant and susceptible classes for AR, the level of PLRV antigen accumulation achieved varied with cultivar. Previous infection with potato virus X (PVX) or potato virus S (PVS) either alone or together did not diminish the expression of IR or AR. However, the presence of PVX sometimes significantly increased the accumulation of PLRV in susceptible cv. Desiree and this effect was most pronounced in mature leaves of older plants. In contrast, presence of PVX in susceptible cv. Desiree did not increase the numbers of plants becoming infected with PLRV. Identifying potato genotypes with and IR AR will help the Australian potato industry to select cultivars which become infected with PLRV more slowly under conditions of high infection pressure and/or are suitable for use as parental lines in breeding virus-resistant cultivars.

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Volunteer potatoes as a source of potato leafroll virus and potato virus X

American Potato Journal ◽

10.1007/bf02853471 ◽

1981 ◽

Vol 58 (11) ◽

pp. 603-609 ◽

Cited By ~ 13

Author(s):

Garrett C. Wright ◽

Guy W. Bishop

Keyword(s):

Potato Virus ◽

Potato Virus X ◽

Potato Leafroll Virus ◽

Leafroll Virus ◽

Volunteer Potatoes

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Resistência de genótipos de batata ao vírus do enrolamento da folha da batata (PLRV) e ao vírus Y (PVY)

Horticultura Brasileira ◽

10.1590/s0102-05362004000300003 ◽

2004 ◽

Vol 22 (3) ◽

pp. 521-524 ◽

Cited By ~ 4

Author(s):

Julio Daniels ◽

Arione da S. Pereira

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Rio Grande ◽

Potato Leafroll Virus ◽

Rio Grande Do Sul ◽

Leafroll Virus ◽

Das Elisa

O vírus do enrolamento da folha da batata (Potato leafroll virus, PLRV) e o vírus Y da batata (Potato virus Y, PVY) constituem as principais causas da degenerescência da batata-semente no Brasil. Com o objetivo de determinar, nas condições do Rio Grande do Sul, a resistência de campo de genótipos de batata à infecção por estes vírus, avaliaram-se, na presença de infectores, durante três plantios consecutivos de primavera, 20 cultivares e clones de batata. A detecção dos vírus foi efetuada por meio de testes sorológicos (DAS-ELISA). Pela análise de agrupamento os genótipos foram separados em três grupos para resistência ao PLRV (Elvira, Achat, Bintje, Monalisa, Monte Bonito, Panda e Araucária, resistentes; Baronesa, Asterix, Atlantic, 2CRI-1149-1-78, C-1226-35-80, Astrid, C-1714-7-94, A-1139-12-92, Macaca, Eliza e Santo Amor, suscetíveis; Catucha e Cristal, muito suscetíveis) e em quatro grupos para resistência ao PVY (Asterix, Astrid, Catucha, Cristal, Macaca, Monte Bonito, A-1139-12-92, C-1226-35-80 e C-1714-7-94, resistentes; Baronesa, Santo Amor, Monalisa, Panda e 2CRI-1149-1-78, resistentes intermediários; Bintje, Atlantic, Elvira e Araucária, suscetíveis; Achat e Eliza, muito suscetíveis).

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Multiple DNA markers for evaluation of resistance against Potato virus Y, Potato virus S and Potato leafroll virus

Czech Journal of Genetics and Plant Breeding ◽

10.17221/180/2016-cjgpb ◽

2018 ◽

Vol 54 (No. 1) ◽

pp. 30-33 ◽

Cited By ~ 2

Author(s):

M. Naderpour ◽

L. Sadeghi

Keyword(s):

Potato Virus ◽

Potato Virus Y ◽

Simultaneous Detection ◽

Potato Leafroll Virus ◽

Potato Seed ◽

Breeding Programs ◽

Potato Virus S ◽

Sequence Characterized Amplified Region ◽

Leafroll Virus ◽

Resistance To Viruses

Molecular markers within or close to genes of interest play essential roles in marker-assisted selection. PCR-based markers have been developed for numerous traits in different plant species including several genes conferring resistance to viruses in potato. In the present work, rapid and reliable approaches were developed for the simultaneous detection of Ryadg and Ry-fsto, Ns, and PLRV.1 genes conferring resistance to Potato virus Y, Potato virus S and Potato leafroll virus, respectively, on the basis of previously published and newly modified markers. The sequence characterized amplified region (SCAR) markers for Ryadg, Ns and PLRV1 and the newly modified cleaved amplified polymorphic sequences (CAPS) marker for Ry-fsto were amplified in one PCR reaction which could simply characterize Ryadg and PLRV.1 resistance. Additional digestion of amplicons with EcoRV and MfeI for genotyping the Ry-fsto and Ns resistance genes, respectively, was needed. The effectiveness of genotyping in triplex and tetraplex PCRs was tested on 35 potato varieties used for potato seed production and breeding programs.

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