Use of Reciprocal Grafting to Study Brown Stem Rot Resistance in Soybean

Resistance to brown stem rot of soybean has been identified and utilized in cultivar development and germ plasm enhancement. However, little is known about the nature of resistance to this disease. In an attempt to better understand the resistance mechanism by locating the tissue or tissues responsible for resistance, reciprocal grafts were made between brown stem rot-resistant and -susceptible genotypes including near-isogenic lines for resistance gene Rbs2. Results of brown stem rot evaluation of grafted plants indicate that brown stem rot resistance is conditioned by the root in all resistance sources evaluated.

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A LINKAGE MAP OF THE TOMATO SPOTTED WILT VIRUS RESISTANCE GENE SW-5 USING NEAR ISOGENIC LINES AND AN INTERSPECIFIC CROSS

Acta Horticulturae ◽

10.17660/actahortic.1996.431.33 ◽

1996 ◽

pp. 385-392 ◽

Cited By ~ 5

Author(s):

M.R. Stevens ◽

D.K. Heiny ◽

D.D. Rhoads ◽

P.D. Griffiths ◽

J.W. Scott

Keyword(s):

Linkage Map ◽

Resistance Gene ◽

Virus Resistance ◽

Tomato Spotted Wilt Virus ◽

Interspecific Cross ◽

Near Isogenic Lines ◽

Isogenic Lines ◽

Tomato Spotted Wilt ◽

Wilt Virus ◽

Virus Resistance Gene

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Resistance to Brown Stem Rot in Soybean Germ Plasm with Resistance to the Soybean Cyst Nematode

Plant Disease ◽

10.1094/pdis.2004.88.7.761 ◽

2004 ◽

Vol 88 (7) ◽

pp. 761-768 ◽

Cited By ~ 11

Author(s):

T. J. Hughes ◽

N. C. Kurtzweil ◽

B. W. Diers ◽

C. R. Grau

Keyword(s):

Soybean Cyst Nematode ◽

Field Experiments ◽

Germ Plasm ◽

Cyst Nematode ◽

Stem Rot ◽

Brown Stem Rot ◽

Resistant Cultivars ◽

Greenhouse Experiments ◽

Scn Resistance ◽

Pi 88788

The soybean cyst nematode (SCN) and Phialophora gregata f. sp. sojae, the causal agent of brown stem rot (BSR), are two pathogens of soybean commonly found in the same field throughout the north-central United States. Field experiments designed to study the role of SCN-resistant germ plasm in soybean production have led to data suggesting that some sources of SCN resistance also may provide resistance to BSR. Soybean germ plasm with resistance to SCN was evaluated in greenhouse and field environments for resistance to BSR development based on the percentage of host tissue symptomatic of BSR. Comparison of SCN-resistant cultivars and plant introductions (PI) to standard BSR-resistant and -susceptible checks were conducted in two greenhouse experiments using a root-dip inoculation with a single isolate of P. gregata. For both greenhouse experiments, PI 209332 was the only source of SCN resistance with resistance to BSR similar to standard BSR-resistant checks. Nine other sources of SCN resistance, including PI 88788 and Peking, expressed BSR symptom severity similar to BSR-susceptible checks. Cultivars derived from most SCN-resistant sources, including PI 209332, also were susceptible to BSR development, while four of the five cultivars derived from PI 88788 were highly resistant to BSR development. SCN-resistant cultivars derived from PI 88788, Peking, and PI 209332 were planted along with standard BSR-resistant and -susceptible checks at two field locations naturally infested with P. gregata and SCN or P. gregata alone. As in greenhouse experiments, four of the five cultivars derived from PI 88788 expressed resistance to BSR development equal to or better than standard BSR-resistant checks at both locations. In contrast, cultivars derived from PI 209332 and Peking expressed varying levels of disease development depending on field environment. Yields observed for PI 88788-derived cultivars were higher than BSR-resistant checks regardless of the presence of SCN. Data from both greenhouse and field experiments suggest that cvs. Williams and Williams 82 may contain a gene or genes for BSR resistance that require one or more modifier genes, possibly located in the genome of PI 88788, for complete expression.

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Near Isogenic Lines Confirm a Soybean Cyst Nematode Resistance Gene from PI 88788 on Linkage Group J

Crop Science ◽

10.2135/cropsci2004.0936 ◽

2004 ◽

Vol 44 (3) ◽

pp. 936 ◽

Cited By ~ 13

Author(s):

K. D. Glover ◽

D. Wang ◽

P. R. Arelli ◽

S. R. Carlson ◽

S. R. Cianzio ◽

...

Keyword(s):

Linkage Group ◽

Resistance Gene ◽

Soybean Cyst Nematode ◽

Nematode Resistance ◽

Cyst Nematode ◽

Near Isogenic Lines ◽

Isogenic Lines ◽

Nematode Resistance Gene ◽

Soybean Cyst Nematode Resistance ◽

Pi 88788

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Does phloem-based resistance to aphid feeding affect host-plant acceptance for reproduction? Parturition of the pea aphid, Acyrthosiphon pisum, on two near-isogenic lines of Medicago truncatula

Bulletin of Entomological Research ◽

10.1017/s0007485313000370 ◽

2013 ◽

Vol 103 (6) ◽

pp. 683-689 ◽

Cited By ~ 3

Author(s):

K. Jung Nam ◽

G. Powell ◽

J. Hardie

Keyword(s):

Medicago Truncatula ◽

Acyrthosiphon Pisum ◽

Resistance Mechanism ◽

Pea Aphid ◽

Host Recognition ◽

Near Isogenic Lines ◽

Electrical Penetration Graph ◽

Isogenic Lines ◽

Aphid Clone ◽

Plant Acceptance

AbstractProbing behaviour (prior to parturition) and parturition of two clones (PS01 and N116) of the pea aphid, Acyrthosiphon pisum on two genotypes (near-isogenic lines (NILs)) (Q174_5.13 and Q174_9.10) of Medicago truncatula were investigated using electrical penetration graph (EPG) coupled with simultaneous visual monitoring for parturition. Line Q174_5.13 has been reported to show a phloem-based resistance to feeding in the clone PS01 but to be susceptible to the clone N116, whereas Q174_9.10 has shown to be susceptible to both aphid clones. The time taken to first parturition by clone PS01 was similar on Q174_5.13 and Q174_9.10. Prior to parturition, no aphids on Q174_5.13 contacted phloem, but 5% of the aphids on Q174_9.10 showed phloem salivation (recognized by EPG pattern E1). No phloem contact was observed with aphid clone N116 on either NILs of Medicago before first parturition occurred, and the time taken to first larviposition was similar on Q174_5.13 and Q174_9.10. The results indicate that the initiation of parturition of the clone PS01 and N116 on both NILs does not require the phloem contact and seems unchanged by a phloem-based resistance mechanism to feeding on Medicago. This finding suggests that host recognition and decisions about parturition occur before phloem contact or ingestion, and act independently on R-gene-mediated resistance.

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Deciphering signalling network in broad spectrum Near Isogenic Lines of rice resistant to Magnaporthe oryzae

Scientific Reports ◽

10.1038/s41598-019-50990-8 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Priyanka Jain ◽

Himanshu Dubey ◽

Pankaj Kumar Singh ◽

Amolkumar U. Solanke ◽

Ashok K. Singh ◽

...

Keyword(s):

Resistance Gene ◽

Broad Spectrum ◽

Magnaporthe Oryzae ◽

Blast Resistance ◽

Plant Secondary Metabolite ◽

Common Mechanism ◽

Transcriptional Level ◽

Near Isogenic Lines ◽

Blast Resistance Gene ◽

Isogenic Lines

AbstractDisease resistance (R) genes like Pi9, Pita, Pi21, Pi54 are playing important role for broad spectrum blast resistance in rice. Development of near isogenic lines (NILs) using these type of broad spectrum genes and understanding their signalling networks is essential to cope up with highly evolving Magnaporthe oryzae strains for longer duration. Here, transcriptional-level changes were studied in three near-isogenic lines (PB1 + Pi1, PB1 + Pi9 and PB1 + Pi54) of rice resistant to blast infection, to find the loci that are unique to resistant lines developed in the background of Pusa Basmati 1 (PB1). The pathway analysis of loci, unique to resistant NILs compared to susceptible control revealed that plant secondary metabolite synthesis was the common mechanism among all NILs to counter against M. oryzae infection. Comparative transcriptome analysis helped to find out common clusters of co-expressed significant differentially expressed loci (SDEL) in both PB1 + Pi9 and PB1 + Pi54 NILs. SDELs from these clusters were involved in the synthesis and degradation of starch; synthesis and elongation of fatty acids; hydrolysis of phospholipids; synthesis of phenylpropanoid; and metabolism of ethylene and jasmonic acid. Through detailed analysis of loci specific to each resistant NIL, we identified a network of signalling pathways mediated by each blast resistance gene. The study also offers insights into transcriptomic dynamics, points to a set of important candidate genes that serve as module to regulate the changes in resistant NILs. We suggest that pyramiding of the blast resistance gene Pi9 with Pi54 will lead to maximum broad spectrum resistance to M. oryzae.

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Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines.

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.88.6.2336 ◽

1991 ◽

Vol 88 (6) ◽

pp. 2336-2340 ◽

Cited By ~ 262

Author(s):

G. B. Martin ◽

J. G. Williams ◽

S. D. Tanksley

Keyword(s):

Resistance Gene ◽

Rapid Identification ◽

Near Isogenic Lines ◽

Isogenic Lines ◽

Random Primers

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Gene Expression Patterns in Near Isogenic Lines for Wheat Rust Resistance Gene Lr34/Yr18

Phytopathology ◽

10.1094/phyto-97-9-1083 ◽

2007 ◽

Vol 97 (9) ◽

pp. 1083-1093 ◽

Cited By ~ 58

Author(s):

S. H. Hulbert ◽

J. Bai ◽

J. P. Fellers ◽

M. G. Pacheco ◽

R. L. Bowden

Keyword(s):

Leaf Rust ◽

Resistance Gene ◽

Yellow Rust ◽

Expression Patterns ◽

Defense Responses ◽

Pr Proteins ◽

Adult Plant ◽

Near Isogenic Lines ◽

Flag Leaves ◽

Isogenic Lines

The Lr34/Yr18 resistance gene provides durable, adult-plant, slow rusting resistance to leaf rust, yellow rust, and several other diseases of wheat. Flag leaves may exhibit spontaneous leaf tip necrosis and tips are more resistant than leaf bases. Despite the importance of this gene, the mechanism of resistance is unknown. Patterns of expression for 55,052 transcripts were examined by microarray analysis in mock-inoculated flag leaves of two pairs of wheat near isogenic lines for Lr34/Yr18 (Jupateco 73S/Jupateco 73R and Thatcher/Thatcher-Lr34). The Thatcher isolines were also examined for patterns of expression after inoculation with leaf rust. Mock-inoculated leaf tips of resistant plants showed up-regulation of 57 transcripts generally associated with ABA inducibility, osmotic stress, cold stress, and/or seed maturation. Several transcripts may be useful as expression markers for Lr34/Yr18. Five transcripts were also up-regulated in resistant leaf bases. The possible role of these transcripts in resistance is discussed. In mock-inoculated plants, pathogenesis-related (PR) proteins were not up-regulated in resistant flag leaves compared with that in susceptible flag leaves. In inoculated plants, the same set of PR proteins was up-regulated in both resistant and susceptible flag leaves. However, expression was often higher in resistant plants, suggesting a possible role for Lr34/Yr18 in priming of defense responses.

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